ES8200815A1 - Inactivating Inhibitors in Endotoxin Clottable Extracts - Google Patents

Inactivating Inhibitors in Endotoxin Clottable Extracts

Info

Publication number
ES8200815A1
ES8200815A1 ES494355A ES494355A ES8200815A1 ES 8200815 A1 ES8200815 A1 ES 8200815A1 ES 494355 A ES494355 A ES 494355A ES 494355 A ES494355 A ES 494355A ES 8200815 A1 ES8200815 A1 ES 8200815A1
Authority
ES
Spain
Prior art keywords
endotoxin
extract
clottable
extracts
inactivating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES494355A
Other languages
Spanish (es)
Other versions
ES494355A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Baxter Travenol Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Travenol Laboratories Inc filed Critical Baxter Travenol Laboratories Inc
Publication of ES494355A0 publication Critical patent/ES494355A0/en
Publication of ES8200815A1 publication Critical patent/ES8200815A1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Endotoxin clottable extracts are standardized by inactivating endogenous inhibitor by adjusting the extract pH to above 8.5, incubating and readjusting the pH to near neutrality, determining the coagulase activity of the extract and dividing the extract into endotoxin assay reaction mixture portions each providing coagulase activity at a predetermined level. In most circumstances the coagulogen concentration is also determined and, if the concentration is lower than a predetermined amount, coagulogen is added to the extract.
ES494355A 1979-09-14 1980-08-19 Inactivating Inhibitors in Endotoxin Clottable Extracts Expired ES8200815A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7557979A 1979-09-14 1979-09-14

Publications (2)

Publication Number Publication Date
ES494355A0 ES494355A0 (en) 1981-11-16
ES8200815A1 true ES8200815A1 (en) 1981-11-16

Family

ID=22126689

Family Applications (1)

Application Number Title Priority Date Filing Date
ES494355A Expired ES8200815A1 (en) 1979-09-14 1980-08-19 Inactivating Inhibitors in Endotoxin Clottable Extracts

Country Status (7)

Country Link
JP (1) JPS5642590A (en)
CA (1) CA1153714A (en)
DE (1) DE3034484A1 (en)
ES (1) ES8200815A1 (en)
FR (1) FR2465002B1 (en)
GB (1) GB2059583B (en)
IT (1) IT1212433B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5885162A (en) * 1981-11-16 1983-05-21 Seikagaku Kogyo Co Ltd Endotoxine measurement
JP3524120B2 (en) * 1992-05-08 2004-05-10 生化学工業株式会社 Pretreatment agent, pretreatment method, measurement method using pretreated sample, measurement kit, and sample determination method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4096091A (en) * 1974-07-17 1978-06-20 Baxter Travenol Laboratories, Inc. Limulus lysate having improved capacity to precipitate in the presence of low endotoxin concentrations, and reconstituting solutions therefor
US4107077A (en) * 1975-07-14 1978-08-15 Associates Of Cape Cod, Inc. Limulus lysate of improved sensitivity and preparing the same
US4038029A (en) * 1976-01-20 1977-07-26 Worthington Biochemical Corporation Limulus lysate turbidity test for pyrogens
US4038147A (en) * 1976-06-15 1977-07-26 Becton, Dickinson And Company Method for detecting endotoxins

Also Published As

Publication number Publication date
FR2465002A1 (en) 1981-03-20
FR2465002B1 (en) 1985-06-14
ES494355A0 (en) 1981-11-16
IT8024649A0 (en) 1980-09-12
GB2059583A (en) 1981-04-23
JPH0141320B2 (en) 1989-09-05
IT1212433B (en) 1989-11-22
JPS5642590A (en) 1981-04-20
DE3034484A1 (en) 1981-04-02
CA1153714A (en) 1983-09-13
GB2059583B (en) 1983-06-08

Similar Documents

Publication Publication Date Title
Sun Growth stimulation of 3T3 fibroblasts by cystatin
DE3687328D1 (en) ON-SITE DETERMINATION OF TOTAL CARBON CONTENT FOR EVALUATION OF NUTRESTONE.
NO166465C (en) PROCEDURE FOR DETERMINING THE SEQUENCE OF MONOMERS.
DK400181A (en) COMPOSITION MATERIALS CONTAINING IMMOBILIZED BIOLOGICALLY ACTIVE SPECIES PROCEDURE FOR THEIR PREPARATION AND THEIR USE
GB1448613A (en) Method of detecting immunoglobulin or antigens -or haptens-
CA2167048A1 (en) Identification of ligands by selective amplification of cells transfected with receptors
MY108526A (en) Monoclonal antibodies against human interleukin-4 and hybridomas producing the same
ES8107310A1 (en) Chromogenic compounds and their use as enzymatic substrates.
ES8801037A1 (en) Method for the quantitative determination of protein-C and an activating preparation for its implementation.
AU673478B2 (en) Assay for the determination of protein in a biological sample
DK516585A (en) SV40 EXPRESSION VECTOR CONTAINING HBXAG-AS EXPRESSION MARKS
MX162710A (en) IMPROVEMENTS IN SHUT-OFF VALVE / COMPENSATION
ES8700690A1 (en) Plant cells resistant to herbicidal glutamine synthetase inhibitors.
IT1157304B (en) DETERMINATION OF IMMUNOCHEMISTRY ENZYMES
ES8200815A1 (en) Inactivating Inhibitors in Endotoxin Clottable Extracts
Thomas et al. Differential effects of horse ATG and rabbit ATG on T cell and T cell subset levels measured by monoclonal antibodies
ES8609728A1 (en) Stabilized enzyme conjugate composition.
ATE11930T1 (en) IN VITRO METHOD FOR DETERMINING CANCER IN MAMMALIAN TISSUE.
ATE71149T1 (en) MONOCLONAL ANTIBODIES.
ES2052913T3 (en) IMMUNOCHEMICAL TEST AS TO THE STIMULATING FACTOR OF GRANULOCITOS-MACROFAGOS COLONIES.
MY103022A (en) Monoclonal antibodies recognizing l-thyroxine
EP0174026A3 (en) Nonspecific-reaction inhibitor in an immunological assay with monoclonal antibody
Jack et al. External Corrosion of Line Pipe. II. Laboratory Study of Cathodic Protection in the Presence of Sulphate-Reducing Bacteria
IT1111665B (en) METHODS AND REAGENTS FOR THE ANALYSIS OF IRON AND IRON BINDING PROTEINS
ATE41953T1 (en) INCREASED INHIBITION OF YEAST CELL GROWTH.