ES455435A1 - Method of obtaining and using antigen from neisseria gonorrheae and products prepared therefrom - Google Patents

Method of obtaining and using antigen from neisseria gonorrheae and products prepared therefrom

Info

Publication number
ES455435A1
ES455435A1 ES455435A ES455435A ES455435A1 ES 455435 A1 ES455435 A1 ES 455435A1 ES 455435 A ES455435 A ES 455435A ES 455435 A ES455435 A ES 455435A ES 455435 A1 ES455435 A1 ES 455435A1
Authority
ES
Spain
Prior art keywords
antigen
acid
cells
regulator
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES455435A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of ES455435A1 publication Critical patent/ES455435A1/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/12—Nitrate to nitrite reducing bacteria

Abstract

1. A process for obtaining surface or membrane-associated antigens from whole cells or culture media in which they are grown, characterized in that it comprises: a) destroying and fixing said cells; (b) separating said cells from the medium; (c) extracting antigen from said cells or culture medium with an alkaline aqueous solution of ph regulated alkali metal salt having a ph of about 6.0 to about 11.0, wherein the concentration of said ph regulator and salt is at least 0.01 mole; d) concentrating said antigen; (e) acid precipitating said concentrated antigen with an aqueous acid or an acid buffer solution having a ph of from about 1.0 to about 6.0 wherein the concentration of said acid or regulator is at least 0, 01 molar; f) solubilizing said antigen precipitated with acid with an aqueous alkaline solution of regulated ph uqe has a ph of about 6.0 to about 11.0 where the concentration of said regulator is at least 0.01 molar and, if wishes to digest the antigen solubilized with a proteolytic enzyme for about 1/4 hr to about 4hr between about 0ºC and about 45º c; and stop the enzyme digestion of the antigen. (Machine-translation by Google Translate, not legally binding)
ES455435A 1976-01-29 1977-01-28 Method of obtaining and using antigen from neisseria gonorrheae and products prepared therefrom Expired ES455435A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65367376A 1976-01-29 1976-01-29
US65367276A 1976-01-29 1976-01-29

Publications (1)

Publication Number Publication Date
ES455435A1 true ES455435A1 (en) 1978-05-01

Family

ID=27096562

Family Applications (1)

Application Number Title Priority Date Filing Date
ES455435A Expired ES455435A1 (en) 1976-01-29 1977-01-28 Method of obtaining and using antigen from neisseria gonorrheae and products prepared therefrom

Country Status (15)

Country Link
JP (1) JPS5294419A (en)
AR (1) AR214730A1 (en)
DD (1) DD130435A5 (en)
DE (1) DE2701643A1 (en)
DK (1) DK28077A (en)
ES (1) ES455435A1 (en)
FR (1) FR2339622A1 (en)
GB (1) GB1560570A (en)
GR (1) GR63690B (en)
HU (1) HU174883B (en)
IT (1) IT1086757B (en)
NL (1) NL7700479A (en)
PL (1) PL195632A1 (en)
RO (1) RO70724A (en)
SE (1) SE7700949L (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4203971A (en) * 1978-03-23 1980-05-20 Government Of The United States Neisseria gonorrhoeae vaccine
US4241045A (en) * 1978-05-15 1980-12-23 Research Corporation Purified antigen to test for Neisseria gonorrheae antibodies
US4330623A (en) * 1980-02-19 1982-05-18 Merck & Co., Inc. Process for solubilization of gonococcal antigen
WO1983003354A1 (en) * 1982-03-30 1983-10-13 Us Army Neisseria gonorrhoeae vaccine
US4851337A (en) * 1986-01-08 1989-07-25 Hygeia Sciences, Inc. Extraction of test substances
US5122449A (en) * 1988-10-07 1992-06-16 Eastman Kodak Company Use of a protease in the extraction of chlamydial, gonococcal and herpes antigens
US5399484A (en) * 1991-10-08 1995-03-21 Eastman Kodak Company Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor
GB9315769D0 (en) * 1993-07-30 1993-09-15 Kodak Ltd Method of photographic processing

Also Published As

Publication number Publication date
RO70724A (en) 1982-10-11
NL7700479A (en) 1977-08-02
AR214730A1 (en) 1979-07-31
IT1086757B (en) 1985-05-31
DD130435A5 (en) 1978-03-29
DK28077A (en) 1977-07-30
FR2339622A1 (en) 1977-08-26
SE7700949L (en) 1977-10-10
HU174883B (en) 1980-04-28
PL195632A1 (en) 1978-12-04
GR63690B (en) 1979-11-29
JPS5294419A (en) 1977-08-09
DE2701643A1 (en) 1977-08-04
GB1560570A (en) 1980-02-06

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