ES395956A1 - Triglyceride hydrolysis and assay - Google Patents

Triglyceride hydrolysis and assay

Info

Publication number
ES395956A1
ES395956A1 ES295956A ES395956A ES395956A1 ES 395956 A1 ES395956 A1 ES 395956A1 ES 295956 A ES295956 A ES 295956A ES 395956 A ES395956 A ES 395956A ES 395956 A1 ES395956 A1 ES 395956A1
Authority
ES
Spain
Prior art keywords
ester
glycerol
assay
hydrolysis
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES295956A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Chemicals Inc
Original Assignee
Calbiochem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calbiochem Corp filed Critical Calbiochem Corp
Publication of ES395956A1 publication Critical patent/ES395956A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/04—Dairy products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y10S435/897—Streptomyces griseus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/939—Rhizopus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A procedure of analysis of an aqueous liquid containing a fatty acid glycerol ester to determine its content in said ester, in which method the ester is hydrolyzed to release all of the glycerol followed by determination of the amount of glycerol present, characterized by said procedure because it consists in carrying out said hydrolysis by adding a lipase and a protease to said liquid, whereby a substantially complete hydrolysis of said ester takes place. (Machine-translation by Google Translate, not legally binding)
ES295956A 1970-12-16 1971-10-13 Triglyceride hydrolysis and assay Expired ES395956A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9890470A 1970-12-16 1970-12-16

Publications (1)

Publication Number Publication Date
ES395956A1 true ES395956A1 (en) 1974-09-01

Family

ID=22271481

Family Applications (1)

Application Number Title Priority Date Filing Date
ES295956A Expired ES395956A1 (en) 1970-12-16 1971-10-13 Triglyceride hydrolysis and assay

Country Status (17)

Country Link
US (1) US3703591A (en)
JP (2) JPS549518B1 (en)
AU (1) AU475955B2 (en)
BE (1) BE776034A (en)
BR (1) BR7108185D0 (en)
CA (1) CA955161A (en)
CH (2) CH563404A5 (en)
CS (1) CS212729B2 (en)
DE (1) DE2162325C3 (en)
ES (1) ES395956A1 (en)
FR (1) FR2118454A5 (en)
GB (1) GB1373106A (en)
IL (1) IL38235A (en)
IT (1) IT972079B (en)
NL (1) NL180523C (en)
SE (1) SE389919B (en)
ZA (1) ZA717957B (en)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2101796A1 (en) * 1970-01-21 1971-08-05 Baxter Laboratories Inc Method for the determination of glycene in blood serum
AT324282B (en) * 1972-06-19 1975-08-25 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR DETERMINATION OF TRIGLYCERIDES
CS164231B2 (en) * 1972-09-28 1975-11-07
JPS49113695A (en) * 1973-02-27 1974-10-30
AT339505B (en) * 1974-03-14 1977-10-25 Boehringer Mannheim Gmbh ENZYMATIC ANALYSIS PROCEDURE
US3898130A (en) * 1974-03-18 1975-08-05 American Hospital Supply Corp Rapid enzymatic hydrolysis of triglycerides
CA1030436A (en) * 1974-03-20 1978-05-02 William S. Stavropoulos Method for determination of triglycerides and glycerol
US3884764A (en) * 1974-03-25 1975-05-20 Eastman Kodak Co Method and composition for blood serum cholesterol analysis
CA1056282A (en) * 1974-03-25 1979-06-12 Charles T. Goodhue Multilayer analytical elements for use in the assay of cholesterol
JPS50142090A (en) * 1974-04-30 1975-11-15
JPS5169691A (en) * 1974-06-07 1976-06-16 Iatron Lab Ketsuseichuno chuseishibosokuteiyoshaku
US4014744A (en) * 1975-01-30 1977-03-29 Miles Laboratories Inc. Processes for measuring tri-, di- and monoglycerides
US4011045A (en) * 1975-02-14 1977-03-08 Bonderman Dean P Turbidity reduction in triglyceride standards
JPS5217085A (en) * 1975-07-30 1977-02-08 Ono Pharmaceut Co Ltd Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes
US4012287A (en) * 1975-11-18 1977-03-15 Dr. Bruno Lange Gmbh Method and reagent for the quantitative analysis of triglycerides
US4045297A (en) * 1975-12-15 1977-08-30 Monsanto Company Triglycerides determination method
CA1100023A (en) * 1976-08-19 1981-04-28 Charles T. Goodhue Process and composition for the quantification of glycerol and triglycerides
DE2737286C2 (en) * 1976-08-19 1985-05-02 Eastman Kodak Co., Rochester, N.Y. Multi-layer analytical elements for the determination of triglycerides and glycerine in liquids
US4275152A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4275151A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
JPS6058746B2 (en) * 1977-09-22 1985-12-21 中外製薬株式会社 Higher fatty acid ester
US4195126A (en) * 1977-10-04 1980-03-25 The Board Of Trustees Of The University Of Alabama Albumin-dye complex for fatty acid determination
US4245041A (en) * 1977-12-07 1981-01-13 American Monitor Corporation Triglycerides assay and reagents therefor
US4241178A (en) * 1978-01-06 1980-12-23 Eastman Kodak Company Process and composition for the quantification of glycerol ATP and triglycerides
US4223090A (en) * 1978-07-13 1980-09-16 American Hospital Supply Corporation Reagents for the enzymatic determination of triglycerides
DE2831580C2 (en) * 1978-07-18 1980-09-18 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for the determination of glycerin
DE2834704A1 (en) * 1978-08-08 1980-02-21 Boehringer Mannheim Gmbh METHOD FOR THE QUANTITATIVE ENZYMATIC DETERMINATION OF ADP
US4302536A (en) * 1978-08-15 1981-11-24 Longenecker Robert W Colorimetric immunoassay process
US4264589A (en) * 1978-12-20 1981-04-28 Felts James M Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
US4178285A (en) * 1978-12-20 1979-12-11 Felts James M Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
US4394445A (en) * 1979-02-22 1983-07-19 Nix Paul T Enzymatic glyceride hydrolysis
US4259440A (en) * 1979-05-21 1981-03-31 Miles Laboratories, Inc. Hydrolysis and assay of triglycerides
US4322496A (en) * 1980-04-17 1982-03-30 Eastman Kodak Company Inhibition of lactate oxidase
US4309502A (en) * 1980-06-30 1982-01-05 Beckman Instruments, Inc. Enzymatic assay for glycerol and triglycerides and a reagent for use therein
US4338395A (en) * 1980-07-21 1982-07-06 Technicon Instruments Corporation Method for the analysis of triglycerides
EP0045031A1 (en) * 1980-07-22 1982-02-03 Baker Instruments Corporation Glycerol detection reagent and its use
US4394444A (en) * 1982-06-21 1983-07-19 Miles Laboratories, Inc. Cofactor indicator compositions
JPS59140900A (en) * 1983-01-28 1984-08-13 Toyo Jozo Co Ltd Novel method for highly sensitive, enzymatic determination
JPS59196100A (en) * 1983-04-21 1984-11-07 Amano Pharmaceut Co Ltd Glycerol determination and composition therefor
US4626511A (en) * 1985-05-13 1986-12-02 Wayne State University Composition for reducing turbidity in samples of biological fluids
US5310679A (en) * 1985-05-13 1994-05-10 Artiss Joseph D Composition for reducing turbidity in samples of biological fluids
JPWO2002027012A1 (en) * 2000-09-28 2004-06-10 アークレイ株式会社 Method for producing protein hydrolyzate
WO2003029229A1 (en) * 2001-09-28 2003-04-10 Arkray, Inc. Method of storing tetrazolium compound, stabilizer for use therein, and tetrazolium compound reagent solution stored by the method
BR0317746A (en) * 2002-12-24 2005-11-22 Stepan Co Process for determining the glycerine content of a biodiesel sample
US20100296087A1 (en) * 2007-05-31 2010-11-25 Juergen Gailer Method for assessing trace element related disorders in blood plasma
PT2857525T (en) * 2008-02-29 2019-08-05 Univ Oxford Innovation Ltd A method of determining a suitable dosage of statin.
US9817001B2 (en) 2008-05-27 2017-11-14 Boston Heart Diagnostics Corporation Methods for determining LDL cholesterol treatment
US8470541B1 (en) 2008-09-27 2013-06-25 Boston Heart Diagnostics Corporation Methods for separation and immuno-detection of biomolecules, and apparatus related thereto
EP3324184A1 (en) 2011-10-13 2018-05-23 Boston Heart Diagnostics Compositions and methods for treating and preventing coronary heart disease
US20140120559A1 (en) 2012-10-26 2014-05-01 Boston Heart Diagnostics Corporation Diabetes panel
US9828624B2 (en) 2013-07-24 2017-11-28 Boston Heart Diagnostics Corporation Driving patient compliance with therapy
CA2968221A1 (en) 2014-11-17 2016-05-26 Boston Heart Diagnostic Corporation Cardiovascular disease risk assessment

Also Published As

Publication number Publication date
DE2162325A1 (en) 1972-06-22
DE2162325B2 (en) 1980-04-24
AU475955B2 (en) 1976-09-09
NL180523B (en) 1986-10-01
JPS549518B1 (en) 1979-04-25
IT972079B (en) 1974-05-20
ZA717957B (en) 1972-08-30
IL38235A0 (en) 1972-01-27
DE2162325C3 (en) 1980-12-11
NL7117275A (en) 1972-06-20
SE389919B (en) 1976-11-22
BE776034A (en) 1972-03-16
GB1373106A (en) 1974-11-06
AU3641671A (en) 1973-06-07
FR2118454A5 (en) 1972-07-28
NL180523C (en) 1987-03-02
CA955161A (en) 1974-09-24
CS212729B2 (en) 1982-03-26
IL38235A (en) 1974-10-22
US3703591A (en) 1972-11-21
JPS53114493A (en) 1978-10-05
CH563404A5 (en) 1975-06-30
CH566003A5 (en) 1975-08-29
BR7108185D0 (en) 1973-05-31

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19870210