ES2868649T3 - Improved antipilling in polyester textiles through the use of a cutinase - Google Patents

Abstract

Detergent, characterized in that it contains a cutinase, the cutinase being a cutinase that has at least 70% sequence identity with the amino acid sequence indicated in SEQ ID NO: 1 over its entire length.

Description

DESCRIPTION

Improved antipilling in polyester textiles through the use of a cutinase

The present invention belongs to the field of enzyme technology, in particular to the antipilling effect of enzymes such as those used, for example, in detergents. The invention relates to a detergent containing a cutinase as defined herein. The present invention also relates to a method for cleaning textiles and to the use of the agent according to the invention to remove dirt. Furthermore, the invention is directed to the use of a cutinase to reduce the pilling and anti-graying effects in a detergent.

If items of clothing are washed multiple times, they will build up over time. This applies to all types of textiles and is undesirable for the consumer as it makes the garment look older and ugly. Pilling is the name given to the formation of balls or fluff in fabrics. These small fluff appear especially in short staple fabrics. With long, braided fibers, on the other hand, the pilling is less pronounced. These pellets are usually caused by loose fibers in the fabric or those that have come loose from the fabric. Due to their smooth surface, synthetic fibers tend to ball more than natural fibers, because synthetic fibers can detach from the fabric more quickly than rough natural fibers. In the case of woolen fabrics, these fibers "feel" mainly by mechanical friction and form small balls on the surface.

The main effect of pilling is optical. Due to the formation of pilling on the surface, fabrics look quickly worn and older than they are. On the contrary, the functionality of the material is hardly seen or is not affected at all. For example, the constant thinning of the fabric even increases breathability. Pilling is carried out particularly in mechanically stressed areas, mainly in the shoulder and waist areas. Due to the continuous thinning of the fabric, these stressed areas in particular run the risk of forming holes or even tearing.

A technical solution to reduce the pilling effect is cotton textiles. Cellulases are used in the cleaning agent to reduce the pilling effect (DE 69632910 T3). This means that cellulases are used in detergents to show an anti-pilling or anti-graying effect and thus ensure that clothes look new for longer. However, cellulases only have an effect on cotton textiles. For other textiles, such as polyester textiles, there are first approaches of comparable possibilities to reduce pilling. WO 01/92502 A1, WO 2004/059075 A1 and US 5512203 A describe the use of cutinases from Humicola insolens or Pseudomonas mendocina in detergents and cleaning agents.

It is desirable and there is a need for additional and improved solutions that reduce pilling of textiles, particularly textiles containing synthetic fibers such as polyester.

Surprisingly, the inventors of the present invention have discovered that a cutinase from Thermomonospora curvata DSM 43183 shows an anti-pilling effect in the washing process. Compared to detergents without cutinase / PET esterase, tissue paper with prepilling or new fabric has less pilling after 20 washes with a detergent containing the cutinase according to the invention.

The above-described object on which the present application is based is achieved in that a cutinase is added to detergents that has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length. The addition of the cutinase has the effect that textiles washed with the agents according to the invention, in particular polyester textiles, have significantly reduced pilling compared to a control detergent without cutinase.

In a first aspect, the present invention therefore refers to a detergent, characterized in that it contains a cutinase, where the cutinase is a cutinase that has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO : 1 over its entire length.

In a further aspect, the present invention relates to processes for cleaning textiles, characterized in that an agent according to the invention is used in at least one step of the method.

In another aspect, the present invention also relates to the use of a detergent as described herein, with particular preference liquid detergents, to remove soil.

Furthermore, a further aspect of the invention includes the use of a cutinase to reduce the pilling effects of a detergent, particularly preferably a liquid detergent, wherein the agent contains the cutinase, the cutinase being a cutinase having at least 70% sequence identity with the amino acid sequence indicated in SEQ ID NO: 1 over its entire length.

A cutinase (EC 3.1.1.74) or cutin hydrolase is an enzyme that belongs to the a / 13-hydrolases and hydrolyzes cutin. Cutinase is formed, among other things, by some phytopathogenic fungi and bacteria (through cutinase, fungi are able to break the ester bond of cutin in the cuticle of plants and thus penetrate plants).

In various preferred embodiments, the cutinase is a Thermomonospora curvata cutinase, in particular cutinase with the amino acid sequence according to SEQ ID NO: 1, or a cutinase derived therefrom, for example, by mutagenesis.

In several additional embodiments, the medium is characterized in that

(a) the cutinase can be obtained from a cutinase as defined above as the starting molecule by one or more conservative amino acid substitutions; me

(b) the cutinase can be obtained from a cutinase as defined above as the starting molecule by fragmentation, deletion, insertion or substitution mutagenesis and comprises an amino acid sequence that is at least 210, 220, 230 in length, 240, 250, 260, 270, 280 or 289 contiguous amino acids match the starting molecule.

The agents according to the invention contain the cutinase in an amount of 0.00001-1% by weight, preferably in an amount of 0.0001-0.5% by weight, particularly preferably in an amount of 0.001-0.1 % by weight based on active protein.

In various embodiments of the invention, the cutinase comprises an amino acid sequence that is at least 70%, 71%, 72%, 73%, 74%, 75%, 76% of the total length of the amino acid sequence given in the SEQ ID NO: 1, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90, 5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5% , 97%, 97.5%, 98%, 98.5% and 98.8% identical.

The identity of the amino acid or nucleic acid sequences is determined by a sequence comparison. This sequence comparison is based on the established and commonly used BLAST algorithm in the prior art (see, for example, Altschul, SF, Gish, W., Miller, W., Myers, EW & Lipman, DJ (1990) "Basic local alignment search tool. ”J. Mol. Biol. 215: 403-410, and Altschul, Stephan F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Hheng Zhang, Webb Miller, and David J. Lipman ( 1997): "Gapped BLAST and Ps I-BLAST: a new generation of protein database search programs"; Nucleic Acids Res., 25, p. 3389-3402) and basically it happens that similar nucleotide or amino acid sequences in the acid sequences nucleic or amino acid are assigned to each other. A tabular assignment of the relevant positions is called an alignment. Another algorithm available in the prior art is the FASTA algorithm. Sequence comparisons (alignments), in particular multiple sequence comparisons, are created with computer programs. Frequently used, for example, the Clustal series (see, for example, Chenna et al. (2003): Multiple sequence alignment with the Clustal series of programs. Nucleic Acid Research 31, 3497-3500), T-Coffee (compare , for example, Notredame et al. (2000): T-Coffee: A novel method for multiple sequence alignments. J. Mol. Biol. 302, 205-217) or programs based on these programs or algorithms. Sequence comparisons (alignments) are also possible with Vector NTI® Suite 10.3 software (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) with specified standard parameters, whose AlignX module for sequence comparisons is based on ClustalW.

Such a comparison also allows a statement to be made about the similarity of the compared sequences to each other. It is usually given in percent identity, that is, the proportion of identical nucleotides or amino acid residues at the same positions or positions that correspond to each other in an alignment. The broader term homology includes conserved amino acid exchanges in the case of amino acid sequences, that is, amino acids with similar chemical activity, since these generally exert similar chemical activities within the protein. Thus, the similarity of the compared sequences can also be given as percent homology or percent similarity. Identity and / or homology information can be generated on whole polypeptides or genes or only on individual areas. Thus, homologous or identical regions of different amino acid or nucleic acid sequences are defined by sequence coincidences. These areas usually have identical functions. They can be small and only contain a few nucleotides or amino acids. These small areas often have essential functions for the overall activity of the protein. Therefore, it can be useful to relate sequence matches only to individual, possibly small areas. However, unless otherwise indicated, the details of identity or homology in the present application refer to the total length of the nucleic acid or amino acid sequence specified in each case.

In a further embodiment of the invention, the cutinase is characterized in that its antipilling performance is not significantly reduced compared to that of a cutinase comprising an amino acid sequence corresponding to the amino acid sequences given in SEQ ID NO: 1, i.e. , at least 70%, 75%, 80%, 85%, 90%, 95% of the reference antipilling performance. The antipilling performance can be determined in a washing system that contains a detergent in a dose between 4.5 and 7.0 grams per liter of washing liquor as well as the cutinase, using the cutinases to be compared in the same concentration (based on active protein) and antipilling performance is determined as described herein. For example, the washing process can take place for 60 minutes at a temperature of 60 ° C and the water has a hardness between 15.5 and 16.5 ° (German hardness). The cutinase concentration in the detergent intended for this washing system is 0.00001 to 1% by weight, preferably 0.0001 to 0.5% by weight, especially preferably 0.001 to 0.1% by weight , referred to the purified active protein.

A preferred liquid detergent for such a washing system is composed as follows (all data in percent by weight): 4.4% alkylbenzenesulfonic acid, 5.6% anionic surfactants, 2.4% sodium salts of acids ^{C 12} -C ¹⁸ fatty acids, 4.4% non-ionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamer, 2% glycerin, 0.08% preservatives, 1% ethanol, 1.6% enzyme mixture (protease, amylase, cellulase, manase), rest of demineralized water. The dosage of the liquid detergent is preferably between 4.5 and 6.0 grams per liter of wash liquor, for example 4.7, 4.9 or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.

In the context of the invention, the anti-pilling performance is determined at 60 ° C using a liquid detergent as indicated above, the washing process preferably taking place for 60 minutes.

Anti-pilling performance can be tracked based on visual sampling. In this case, a group of examiners assigns to the clothes to be examined a value on a scale of 1 to 5. The value = 1 means clothes with a lot of pilling, while the value = 5 is assigned to clothes without pilling.

The use of the respective cutinase with the same activity ensures that the respective enzyme properties, eg anti-pilling performance, are compared even if there is a gap in the ratio of active substance to total protein (the activity values). In general, a low specific activity can be compensated for by adding more protein.

Proteins can be combined into groups of immunologically related proteins by reacting with a specific antiserum or antibody. Members of such a group are distinguished by the fact that they have the same antigenic determinant recognized by an antibody. Therefore, they are structurally so similar to each other that they can be recognized by an antiserum or certain antibodies. Therefore, another object of the invention are cutinases, which are characterized by having at least one and increasingly preferably two, three or four antigenic determinants coinciding with a cutinase used in an agent according to the invention. Due to their immunological similarities, such cutinases are structurally so similar to the cutinases used in the agents according to the invention that a similar function can be assumed.

Other cutinases used in the agents according to the invention may have more amino acid changes, in particular amino acid substitutions, insertions or deletions, compared to the cutinase described in SEQ ID NO: 1. Said cutinases are further developed, for example, by modification Genetically directed, i.e. by mutagenesis processes, and they are optimized for specific purposes or with respect to special properties (eg with respect to their catalytic activity, stability, etc.). Furthermore, the nucleic acids encoding the cutinases used can be introduced into recombination batches and thus used to generate completely new types of cutinases or other polypeptides.

The aim is to introduce targeted mutations such as substitutions, insertions or deletions in the known molecules in order, for example, to improve the cleaning capacity of the enzymes according to the invention. For this, in particular, the surface charges and / or the isoelectric point of the molecules and, therefore, their interactions with the substrate, can be modified. For example, the net charge of enzymes can be changed to influence substrate binding, especially for use in detergents and cleaning agents. Alternatively or in addition, one or more corresponding mutations can further increase the stability of the cutinase and thus improve its cleaning ability. Advantageous properties of individual mutations, eg individual substitutions, can complement each other. Therefore, a cutinase that has already been optimized with respect to certain properties, for example with respect to its activity and / or its anti-pilling behavior, can be further developed within the scope of the invention.

Therefore, another object of the invention is an agent containing a cutinase, which is characterized in that it can be obtained from a cutinase as described above as a starting molecule by single or multiple conservative amino acid substitutions. The term "conservative amino acid substitution" means the exchange (substitution) of an amino acid residue for another amino acid residue, this exchange does not lead to a change in polarity or charge at the position of the exchanged amino acid, for example, the exchange of one non-polar amino acid residue for another non-polar amino acid residue. Conservative amino acid substitutions within the scope of the invention include, for example: G = A = S, I = V = L = M, D = E, N = Q, K = R, Y = F, S = T, G = A = I = V = L = M = Y = F = W = P = S = T.

Alternatively or additionally, the cutinase is characterized in that it can be obtained from a cutinase contained in an agent according to the invention as a starting molecule by fragmentation, deletion, insertion or substitution mutagenesis and comprises an amino acid sequence corresponding to the starting molecule in a length of at least 210, 220, 230, 240, 250, 260, 270, 280 or 289 contiguous amino acids.

For example, it is possible to remove individual amino acids at the ends or loops of the enzyme without loss or reduction of hydrolytic activity as a result. Furthermore, such fragmentation, deletion, insertion or substitution mutagenesis can also reduce, for example, the allergenicity of the affected enzymes and thus improve their overall utility. Advantageously, the enzymes retain their hydrolytic activity even after mutagenesis, that is, their hydrolytic activity corresponds at least to that of the starting enzyme, that is, in a preferred embodiment the hydrolytic activity is at least 80, preferably at least 90% of the activity of the starting enzyme. Other substitutions can also have advantageous effects. Both individual and multiple amino acids can be exchanged for other amino acids.

All the percentages given in relation to the compositions described herein refer, unless explicitly stated otherwise, to% by weight, in each case based on the mixture in question.

In the context of the present invention, fatty acids or fatty alcohols or their derivatives, unless otherwise indicated, represent branched or unbranched carboxylic acids or alcohols or their derivatives preferably having 6 to 22 carbon atoms. In particular, oxoalcohols or their derivatives which can be obtained, for example, by ROELEN oxosynthesis, or their derivatives can also be used accordingly. Provided that alkaline earth metals are mentioned below as counter ions for monovalent anions, this means that of course the alkaline earth metal is only present in half the amount of substance as the anion, enough to balance the charge.

An object of the invention is a detergent characterized in that it contains a cutinase as defined herein.

This object of the invention includes all conceivable types of detergent, both concentrates and agents for use without dilution, for use on a commercial scale, in the washing machine or in hand washing. These include, for example, detergents for textiles, carpets or natural fibers, for which the term detergent is used. Detergents within the scope of the invention also include laundry aids that are added to the actual detergent in manual or machine washing to achieve an additional effect. Furthermore, laundry detergents in the context of the invention also include textile pre-treatment and post-treatment agents, that is, those agents with which the garment comes into contact prior to actual washing, for example, to loosen dirt. Persistent, and also the agents that are used in a step that follows the washing of the actual fabric, imparts other desirable properties to the clothing, such as a pleasant touch, no wrinkles or low static load. Fabric softeners, among others, are included in the latter medium.

The detergents according to the invention, which can be present as powdery solids, in the form of post-compacted particles, as homogeneous solutions or suspensions, can contain, in addition to the cutinase described above, all the common known ingredients in such agents, with at least one ingredient which is preferably present in the agent. The agents according to the invention may contain in particular surfactants, adjuvants, bleaching agents, in particular peroxygen compounds or bleach activators. They may also contain water-miscible organic solvents, other enzymes, sequestering agents, electrolytes, pH regulators and / or additional auxiliaries such as optical brighteners, aging inhibitors, foam regulators and dyes and fragrances and combinations thereof.

In particular, a combination of the agent according to the invention with one or more additional ingredients is advantageous, since said agent in preferred embodiments according to the invention has an improved cleaning power through the resulting synergisms. This synergism can be achieved in particular by combining the agent according to the invention with a surfactant and / or an adjuvant and / or a peroxygen compound and / or a bleach activator.

Advantageous ingredients of the agents according to the invention are described in international patent application WO2009 / 121725, which begins on page 5, penultimate paragraph and ends on page 13 after the second paragraph. Express reference is made to this disclosure and the content of the disclosure therein is incorporated into the present patent application.

These and other aspects, features, and advantages of the invention will be apparent to those skilled in the art from a study of the following detailed description and claims. Each feature of one aspect of the invention can be used in any other aspect of the invention. Furthermore, it goes without saying that the examples contained herein are intended to describe and illustrate the invention, but not to restrict it, and in particular the invention is not limited to these examples. Unless otherwise indicated, all percentages are weight percentages based on the total weight of the composition. Numeric ranges given in the "from x to y" format include the indicated values. If several preferred numerical ranges are given in this format, it is understood that all ranges resulting from the combination of the various end points are also recorded.

In addition to cutinase, the agent according to the invention may also contain a compound from the class of anionic surfactants of the formula (I)

R-SO ³ - Y ⁺ (I)

In this formula (I), R represents an unsubstituted linear or branched alkylaryl radical. Y represents a monovalent cation or the n-th part of an n-valent cation, alkali metal ions including Na ⁺ or K ⁺ are preferred herein, with Na ⁺ being extremely preferred. Other Y ⁺ cations can be selected from NH4 ⁺ , A Zn2 ⁺ , 'A Mg2 ⁺ , ' A Ca2 ⁺ , A Mn2 ⁺ and mixtures thereof.

"Alkylaryl", as used herein, refers to organic radicals consisting of an alkyl radical and an aromatic radical. Typical examples of such groups include, but are not limited to, alkylbenzene groups such as benzyl, butylbenzene groups, nonylbenzene groups, decylbenzene groups, undecylbenzene groups, dodecylbenzene groups, tridecylbenzene groups, and the like.

In various embodiments, said surfactants are selected from linear or branched alkyl benzenesulfonates of the formula A-1

Na+

Na +

(A-1),

wherein R 'and R "together contain 9 to 19, preferably 11 to 15 and in particular 11 to 13 carbon atoms. A very particularly preferred representative can be described by the formula A-1a:

In various embodiments, the compound of formula (I) is preferably the sodium salt of a linear alkyl benzenesulfonate.

In the agents according to the invention, the at least one compound of the class of anionic surfactants of the formula (I) is present in an amount of 0.001 to 30% by weight, preferably 0.001 to 10% by weight, more preferably 2 to 6% by weight, more preferably 3-5% by weight, contained in the detergent, each based on the total weight of the detergent.

In various embodiments, the agent may contain at least one other surfactant in addition to or as an alternative to the at least one compound of formula (I). Other anionic surfactants, nonionic surfactants and mixtures thereof, but also cationic, zwitterionic and amphoteric surfactants, are particularly suitable as alternative or additional surfactants.

In various embodiments, the agents according to the invention preferably contain at least one anionic surfactant, in particular an anionic surfactant of the formula R ¹ -O- (AO) ⁿ -SO ³ -X ⁺ (II).

In this formula (II), R ¹ represents a linear or branched, substituted or unsubstituted alkyl, aryl or alkylaryl radical, preferably an unsubstituted linear alkyl radical, especially preferably a fatty alcohol radical. Preferred R ¹ radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals and mixtures thereof, with representatives with an even number of carbon atoms being preferred. Particularly preferred R ¹ radicals are derived from C ¹² -C ¹⁸ fatty alcohols, for example coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl or stearyl alcohol or C ¹⁰ -C ²⁰ oxoalcohols.

AO means an ethylene oxide (EO) or propylene oxide (PO) group, preferably an ethylene oxide group. The index n represents an integer from 1 to 50, preferably from 1 to 20 and in particular from 2 to 10. With very particular preference, n represents the numbers 2, 3, 4, 5, 6, 7 or 8. X means a monovalent cation or the nth part of a n-valent cation, alkali metal ions, including Na + or K +, are preferred, with Na + being extremely preferred. Additional X + cations can be selected from NH4 +, A Zn2 +, 'A Mg2 +, ' A Ca2 +, 'A Mn2 + and mixtures thereof.

In summary, the agents in various embodiments thus contain at least one anionic surfactant selected from fatty alcohol ether sulfates of formula A-2

with k = 11 to 19, n = 2, 3, 4, 5, 6, 7 or 8. Particularly preferred representatives are Na-Ci ² -i ⁴ fatty alcohol ether sulfates with 2 EO (k = 11-13, n = 2 in formula A-2).

In various embodiments, the detergent contains at least one anionic surfactant of formula (II) in an amount of 2-10% by weight, preferably 3-8% by weight, based on the total weight of the detergent.

Other usable anionic surfactants are alkyl sulfates of the formula

R2-O-SOa- X + (III).

In this formula (III), R 2 represents a substituted or unsubstituted linear or branched alkyl radical, preferably an unsubstituted linear alkyl radical, especially preferably a fatty alcohol radical. Preferred R2 radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals, and mixtures thereof, with representatives with an even number of carbon atoms being preferred. Particularly preferred R2 radicals are derived from C ¹² -C ¹⁸ fatty alcohols, for example from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl or stearyl alcohol or from C ¹⁰ -C ²⁰ oxoalcohols. Y represents a monovalent cation or the nth part of an n-valent cation, alkali metal ions including Na + or K + are preferred, with Na + extremely preferred. Other Y + cations can be selected from NH4, A Zn2 +, A Mg2 +, A Ca2 +, A Mn2 +, and mixtures thereof.

In various embodiments, these surfactants are selected from fatty alcohol sulfates of the formula A-3

i— 4 ^ SO "Na +

h, c -hT n ro 3

(A-3)

with k = 11 to 19. Very particularly preferred representatives are Na-C ^12-14 fatty alcohol sulfates (k = 11-13 in formula A-3).

In various embodiments, the agents comprise at least one nonionic surfactant, in particular at least one fatty alcohol alkoxylate. In various embodiments, the detergents contain at least one nonionic surfactant of the formula

R3-O- (AO) m-H (IV),

where

R3 represents a linear or branched, substituted or unsubstituted alkyl radical,

AO means an ethylene oxide (EO) or propylene oxide (PO) group, m represents integers from 1 to 50. In formula (IV) above, R3 represents a linear or branched, substituted or unsubstituted alkyl radical, preferably an unsubstituted linear alkyl radical, particularly preferably a fatty alcohol radical. Preferred R2 radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals, and mixtures thereof, with representatives with an even number of carbon atoms being preferred. Particularly preferred radicals R3 are derived from C ¹² -C ¹⁸ fatty alcohols, for example coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl or stearyl alcohol or C ¹⁰ -C ²⁰ oxoalcohols.

AO means an ethylene oxide (EO) or propylene oxide (PO) group, preferably an ethylene oxide group. The index m represents an integer from 1 to 50, preferably from 1 to 20 and in particular from 2 to 10. Very particularly preferred, m represents the numbers 2, 3, 4, 5, 6, 7 or 8.

In summary, the fatty alcohol alkoxylates that are preferably used are compounds of the formula

with k = 11 to 19, m = 2, 3, 4, 5, 6, 7 or 8. Very particularly preferred representatives are the C12-18 fatty alcohols with 7 EO (k = 11-17, m = 7 in the formula (V)).

Other nonionic surfactants that may be contained in the agents described for the purposes of the present invention include, but are not limited to, alkylglycosides, alkoxylated fatty acid alkyl esters, amine oxides, fatty acid alkanolamides, mixed hydroxyl ethers, esters of sorbitan fatty acids, polyhydric fatty acid amides and alkoxylated alcohols.

Suitable amphoteric surfactants are, for example, betaines of the formula (Riii) (Riv) (Rv) N + CH ² COO ', where Riii is an alkyl radical optionally interrupted by heteroatoms or heteroatoms with 8 to 25, preferably 10 at 21 carbon atoms and Riv and Rv mean identical or different alkyl radicals with 1 to 3 carbon atoms, in particular C ¹⁰ -C ¹⁸ alkyl-dimethylcarboxymethylbetaine and C ¹¹ -C ¹⁷ -alkylamidopropyl-dimethylcarboxymethylbetaine. Suitable cationic surfactants include quaternary ammonium compounds of the formula (Rvi) (Rvii) (Rviii) (Rix) N + X-, where Rvi to Rix for four identical or different alkyl groups, in particular two radicals and X- for an anion, in particular a halide ion, for example didecyldimethylammonium chloride, alkylbenzyldidecylammonium chloride and mixtures thereof. Other suitable cationic surfactants are quaternary surfactant compounds, in particular with a sulfonium, phosphonium, iodonium or arsonium group, which are also known as antimicrobial agents. By using quaternary surfactant compounds with antimicrobial effect, the agent can be designed with antimicrobial effect or its antimicrobial effect, which may already be present due to other ingredients, can be enhanced.

In various embodiments, the total amount of surfactants based on the weight of the agent is 2 to 30% by weight, preferably 5 to 25% by weight, even more preferably 10 to 20% by weight, most preferably 14 to 18% by weight, wherein the (linear) alkylbenzenesulfonates are present in an amount of at most 0.001 to 30% by weight, preferably 0.001-10% by weight, further preferably 2-6% by weight, even more preferably 3- 5% by weight, based on the weight of the agent.

The detergents according to the invention can contain other enzymes in addition to cutinase. These can be hydrolytic enzymes or other enzymes in a concentration suitable for the effectiveness of the agent. Thus, one embodiment of the invention represents agents comprising one or more enzymes. The preferred enzymes that can be used are all the enzymes that can develop a catalytic activity in the agent according to the invention, in particular a protease, amylase, cellulase, hemicellulase, mannanase, tannase, xylanase, xanthanase, xyloglucanase, p-glucosidase, pectinase , carrageenan, perhydrolase, oxidase, oxidoreductase, or a lipase, and mixtures thereof. Advantageously, the enzymes are contained in the agent in an amount of 1 x 10.8 to 5% by weight, based on the active protein. Each enzyme is increasingly preferred in an amount of 1 x 10-7-3% by weight, 0.00001-1% by weight, 0.00005-0.5% by weight, 0.0001 to 0 1% by weight and particularly preferably 0.0001 to 0.05% by weight of agents according to the invention, based on the active protein. The enzymes particularly preferably show a synergistic cleaning capacity against certain soils or stains, that is to say, the enzymes contained in the agent composition mutually support each other in their cleaning power. Synergistic effects can occur not only between different enzymes, but also between one or more enzymes and other ingredients of the agent according to the invention.

The amylase (s) is or is preferably an α-amylase. The hemicellulase is preferably a p-glucanase, a pectinase, a pullulanase and / or a mannanase. The cellulase is preferably a mixture of cellulase or a one-component cellulase, preferably or predominantly an endoglucanase and / or a cellobiohydrolase. The oxidoreductase is preferably an oxidase, in particular a choline oxidase or a perhydrolase.

The proteases used are preferably alkaline serine proteases. They act as nonspecific endopeptidases, that is, they hydrolyze any acid amide bonds found within peptides or proteins and thus break down protein-containing dirt on items to be cleaned. Its optimum pH is usually in the clearly alkaline range.

The protein concentration can be determined using known methods, for example the BCA method (bicinchoninic acid; 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method. In this regard, the active protein concentration is determined by titrating the active sites using a suitable irreversible inhibitor (for proteases, eg, phenylmethylsulfonyl fluoride (PMSF)) and determining the residual activity (cf. M. Bender et al. al., J. Am. Chem. Soc. 88, 24 (1966), pp. 5890-5913).

In the cleaning agents described in this document, the enzymes to be used can also be packaged together with accompanying substances, for example from fermentation. In liquid formulations, the enzymes are preferably used as liquid enzyme formulation (s).

Enzymes are generally not provided in pure protein form, but rather in the form of stabilized, storable, and transportable preparations. These prefabricated preparations include, for example, solid preparations obtained by granulation, extrusion or lyophilization or, in particular in the case of liquid or gelatinous agents, solutions of the enzymes, advantageously as concentrated as possible, with little water and / or with stabilizers. or other auxiliaries.

Alternatively, the enzymes can be encapsulated for both the solid and liquid dosage form, for example, by spray drying or extrusion of the enzyme solution together with a preferably natural polymer or in capsule form, for example those in which the enzymes are enclosed in a solidified gel or in those of the core-layer type, in which an enzyme-containing core is coated with a protective layer impermeable to water, air and / or chemicals. Additional active ingredients, for example stabilizers, emulsifiers, pigments, bleaches or colorants, can also be applied in overlay layers. Said capsules are applied by methods known per se, for example by stirring or granulation by lamination or in fluid bed processes. Advantageously, such granules are low in dust, for example due to the application of polymeric film formers, and due to the coating they are stable in storage.

It is also possible to package two or more enzymes together so that a single granulate has several enzyme activities.

In various embodiments, the agent according to the invention may have one or more enzyme stabilizers. Thus, the agent according to the invention may also contain an enzyme stabilizer selected, for example, from the group consisting of sodium formate, sodium sulfate, lower aliphatic alcohols and boric acid and their esters and salts. Of course, two or more of these compounds can also be used in combination. The salts of the mentioned compounds can also be used in the form of hydrates, such as, for example, sodium sulfate decahydrate.

The term "lower aliphatic alcohols" as used herein includes monoalcohols, diols, and higher alcohols having up to 6 carbon atoms. In this context, polyols, for example glycerol, (mono) ethylene glycol, (mono) propylene glycol or sorbitol may be mentioned as belonging to the group of lower aliphatic alcohols, without the invention being limited thereto.

In addition to the at least one enzyme stabilizer selected from the above group, an agent according to the invention may also contain at least one additional stabilizer. These stabilizers are known in the state of the art.

Reversible protease inhibitors protect the enzymes contained in a detergent from proteolytic degradation by reversibly inhibiting the enzymatic activity of the proteases contained in the agent. Benzamidine hydrochloride, boronic acids or their salts or esters are frequently used as reversible protease inhibitors, including, in particular, derivatives with aromatic groups, such as ortho-, meta-para-substituted phenylboronic acids, in particular 4-formylphenylboronic acid. , or the salts or esters of the mentioned compounds. Peptide aldehydes, ie oligopeptides with a reduced C-terminus, are also used for this purpose, in particular those consisting of 2 to 50 monomers. Reversible peptide protease inhibitors include ovomucoid and leupeptin.

Other enzyme stabilizers are amino alcohols such as mono-, di-, triethanol and propanolamine and their mixtures, aliphatic carboxylic acids up to C ¹² , such as, for example, succinic acid, other dicarboxylic acids or salts of the acids mentioned. End-capped fatty acid amide alkoxylates are also suitable for this purpose. Some organic acids used as adjuvants can also stabilize an enzyme. Calcium and / or magnesium salts, such as calcium acetate, are also used for this purpose. Polyamide oligomers or polymeric compounds such as lignin, water-soluble vinyl copolymers or cellulose ethers, acrylic polymers and / or polyamides stabilize the enzyme preparation against, among other things, physical influences or fluctuations in pH. Polyamine N-oxide containing polymers act simultaneously as enzyme stabilizers and as color transfer inhibitors. Other polymeric stabilizers are linear C ⁸ -C ¹⁸ polyoxyalkylenes. The alkylpolyglycosides can also stabilize the enzymatic components of the agent according to the invention and preferably can further increase their performance. The cross-linked N-containing compounds preferably serve dual functions as soil release agents and as enzyme stabilizers. The hydrophobic nonionic polymer stabilizes in particular any cellulase it may contain.

Reducing agents and antioxidants increase the stability of enzymes against oxidative decomposition; Sulfur-containing reducing agents such as sodium sulfite and reducing sugars are common for this purpose.

In one embodiment, the compositions according to the present invention are liquid and contain water as the main solvent, that is, they are aqueous compositions. The water content of the aqueous agent according to the invention is usually 15 to 70% by weight, preferably 20 to 60% by weight. In various embodiments, the water content is greater than 5% by weight, preferably greater than 15% by weight and particularly preferably greater than 50% by weight, in each case based on the total amount of agent.

In addition, non-aqueous solvents can be added to the agent. Suitable non-aqueous solvents include mono- or polyhydric alcohols, alkanolamines, or glycol ethers, provided they are miscible with water in the specified concentration range. Solvents are preferably selected from ethanol, n-propanol, i-propanol, butanols, glycol, propanediol, butanediol, methylpropanediol, glycerol, diglycol, propyldiglycol, butyldiglycol, hexylene glycol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol ethyl ether, ethylene glycol ethyl ether, ethylene glycol ether, ethylene glycol ethyl ether propyl ether, diethylene glycol methyl ether, diethylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, propylene glycol propyl ether, dipropylene glycol monomethyl ether, dipropylene glycol monoethyl ether, methoxytrigpane glycol, butoxytriglycol-3-butoxytriglycol, butoxytriglycol-3-butoxyglycol-butoxyglycol-3-butoxyriglycol-3 , propylene-3-butoxyethoxy-2-butylene, propylene-butoxy-3-butoxy-3-butanol, propylene-3-butoxy-3-butanol, propylene-3-butoxy-2-butanol, propylene glycol propylether, propylene glycol propylether, 3-butoxyethoxy octyl ether and mixtures of these solvents.

The one or more non-aqueous solvents are normally contained in an amount of 0.1 to 10% by weight, preferably 1 to 8% by weight, based on the total composition.

In addition to the components mentioned above, the agents according to the invention may contain other ingredients that further improve the performance and / or the aesthetic properties of the detergent. These include, for example, additives to improve the drainage and drying behavior, to adjust the viscosity and / or for stabilization, as well as other auxiliaries and additives common in cleaning agents, such as UV stabilizers, perfumes, pearlescent, colorants, corrosion inhibitors, preservatives, bitter substances, organic salts, disinfectants, structuring polymers, defoamers, encapsulated ingredients (eg, encapsulated perfume), pH adjusters and additives that improve or care for the skin.

A detergent according to the invention preferably contains at least one organic and / or inorganic builder (builder), soluble in water and / or insoluble in water.

The builders that can be used in general include in particular aminocarboxylic acids and their salts, zeolites, silicates, carbonates, organic (co) builders and, when there are no ecological damages against their use, also phosphates. Preferably the agents are phosphate free.

Water-soluble organic builder substances include polycarboxylic acids, in particular citric acid and sugar acids, monomeric and polymeric aminopolycarboxylic acids, in particular methylglycinadeacetic acid, nitrilotriacetic acid and ethylenediaminetetraacetic acid as well as polyaspartic acid, polyphosphonic acid, polyphosphonic acid, polyphosphonic acid polyphosphonic acid, 1-methyl-ethoxy-1-methyl-ethoxy-1-methyl-ethane-phosphonic acid, 1-methyl-ethoxy-methylene-phospholylene phosphonic acid and 1-methyl-ethanoic acid, 1-diphosphonic acid, polymeric hydroxy compounds such as dextrin and polymeric (poly) carboxylic acids, polymeric acrylic acids, methacrylic acids, maleic acids and copolymers of these, which may also contain small amounts of polymerizable substances without carboxylic acid functionality as polymerized units. Suitable, although less preferred, compounds of this class are copolymers of acrylic acid or methacrylic acid with vinyl ethers, such as vinyl methyl ethers, vinyl esters, ethylene, propylene and styrene, in which the acid constitutes at least 50% by weight. The organic builders can be used, in particular for the production of liquid agents, in the form of aqueous solutions, preferably in the form of aqueous solutions of 30 to 50 percent by weight. All the acids mentioned are generally used in the form of their water-soluble salts, in particular their alkali metal salts.

If desired, the organic builder substances can be present in amounts of up to 40% by weight, in particular up to 25% by weight and preferably from 1% by weight to 8% by weight. Amounts close to the upper limit mentioned are preferably used in pasty or liquid agents, in particular containing water, according to the invention. Garment after-treatment agents according to the invention, such as fabric softeners, can also optionally be free of organic builders.

Alkali metal silicates and polyphosphates, preferably sodium triphosphate, are particularly suitable as water-soluble inorganic builder materials. As water-insoluble, water-dispersible inorganic builders, in particular crystalline or amorphous alkali aluminosilicates are used, if desired, in amounts of up to 50% by weight. Among these, crystalline sodium aluminosilicates are preferred as detergent, in particular zeolite A, P and optionally X. Amounts close to The upper limit mentioned are preferably used in solid particulate compositions. Particularly suitable aluminosilicates do not have particles with a grain size of more than 30 µm and preferably consist of at least 80% by weight of particles with a size of less than 10 µm.

Suitable substitutes or partial substitutes for said aluminosilicate are crystalline alkali metal silicates, which can be present alone or in mixture with amorphous silicates. The alkali silicates that can be used as builders in the agents according to the invention preferably have a molar ratio of alkali oxide to SiO ^{2 of} less than 0.95, in particular of 1: 1.1 to 1:12, and can be amorphous or crystalline . Preferred alkali silicates are sodium silicates, in particular amorphous sodium silicates, with a Na ² O: SiO ² molar ratio of 1: 2 to 1: 2.8. The crystalline silicates used alone or in mixture with amorphous silicates are preferably crystalline layered silicates of the general formula Na ² Si ^x O ^{2x 1} ■ and H ² O, in which x, the so-called modulus, is a number from 1.9 to 4 and y is a number from 0 to 20 and the preferred values for x are 2, 3 or 4. Preferred crystalline layered silicates are those in which x in the above general formula assumes the values 2 or 3. In particular, it is they prefer beta and delta sodium disilicates (Na ² Si ² O ⁵ ■ and H ² O). The virtually anhydrous crystalline alkali silicates of the above general formula, in which x is a number from 1.9 to 2.1, which are produced from amorphous alkali silicates, can also be used in agents according to the invention. In another preferred embodiment of the agents according to the invention, a crystalline layered sodium silicate with a modulus of 2 to 3 is used, which can be produced from sand and soda. Crystalline sodium silicates with a modulus in the range 1.9 to 3.5 are used in another preferred embodiment of the agents according to the invention. If alkali aluminosilicate, in particular zeolite, is also present as an additional builder, the weight ratio of aluminosilicate to silicate, based in each case on anhydrous active ingredients, is preferably 1:10 to 10: 1. In agents containing both amorphous and crystalline alkali silicates, the weight ratio of amorphous alkali silicate to crystalline alkali silicate is preferably 1: 2 to 2: 1 and in particular 1: 1 to 2: 1.

If desired, the builders are contained in the agents according to the invention preferably in amounts of up to 60% by weight, in particular from 5% by weight to 40% by weight. The clothing after-treatment agents according to the invention, such as fabric softeners, are preferably free of inorganic builders.

Polymeric thickeners in the sense of the present invention are polycarboxylates acting as polyelectrolytes, preferably acrylic acid homo- and copolymers, in particular acrylic acid copolymers, such as acrylic acid-methacrylic acid copolymers, and polysaccharides, in particular heteropolysaccharides, and other common thickening polymers. Suitable polysaccharides or heteropolysaccharides are polysaccharide gums, for example gum arabic, agar, alginates, carrageenans and their salts, guar, guaran, tragacanth, gellan, ramsan, dextran or xanthan and their derivatives, for example propoxylated guar and its mixtures. Other polysaccharide thickeners, such as starches or cellulose derivatives, can be used alternatively, but preferably in addition to a polysaccharide gum, for example starches of various origins and starch derivatives, for example hydroxyethyl starch, starch phosphate esters or starch acetates or carboxymethylcellulose or its sodium salt, methyl, ethyl, hydroxyethyl, hydroxypropyl-, hydroxypropyl-methyl- or hydroxyethyl-methylcellulose or cellulose acetate.

Acrylic acid polymers suitable as polymeric thickeners are, for example, high molecular weight acrylic acid homopolymers (INCI carbomer) crosslinked with a polyalkenyl polyether, in particular an allyl ether of sucrose, pentaerythritol or propylene, which are also called carboxyvinyl polymers.

However, particularly suitable polymeric thickeners are the following acrylic acid copolymers: (i) copolymers of two or more monomers from the group of acrylic acid, methacrylic acid and their simple esters, preferably formed with C ^1-4 alkanols (copolymer of acrylates INCI), which include, for example, the copolymers of methacrylic acid, butyl acrylate and methyl methacrylate (CAS 25035-69-2) or of butyl acrylate and methyl methacrylate (CAS 25852-37-3); (ii) high molecular weight cross-linked acrylic acid copolymers, including copolymers of C ^10-30 alkyl acrylates cross-linked with an allyl ether of sucrose or pentaerythritol with one or more monomers from the group of acrylic acid, methacrylic acid and their simple esters preferably formed with C ^1-4 alkanols, (INCI acrylates / C ^10-30 alkyl acrylate crosspolymer).

The content of the polymeric thickener is normally not more than 8% by weight, preferably between 0.1 and 7% by weight, especially preferably between 0.5 and 6% by weight, in particular between 1 and 5% by weight and most preferably between 1.5 and 4% by weight, for example between 2 and 2.5% by weight, based on the total weight of the detergent.

To stabilize the agent according to the invention, especially with a high content of surfactant, one or more dicarboxylic acids and / or their salts can be added, in particular a composition of Na salts of adipic, succinic and glutaric acids, as described can be obtained under the trade name Sokalan® DSC. They are advantageously used in amounts of 0.1 to 8% by weight, preferably 0.5 to 7% by weight, in particular 1.3 to 6% by weight and especially preferably 2 to 4% by weight, referred to the total weight of the detergent.

However, if its use can be dispensed with, the agent according to the invention is preferably free of (salts) of dicarboxylic acid.

Detergents according to the invention can be compared with reference detergents to determine the increased anti-pilling performance of the agents according to the invention. Said washing system can be composed as follows (all data in percent by weight): reference agent: 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% Na salts C ¹² -C ¹⁸ Fatty Acids (Soaps), 4.4% Nonionic Surfactants, 0.2% Phosphonates, 1.4% Citric Acid, 0.95% NaOH, 0.01% Defoamer, 2 , 0% glycerin, 0.08% preservatives, 1% ethanol, 1.6% enzyme mixture (protease, amylase, cellulase, manase), the rest demineralized water. Agent according to the invention: 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% Na C ¹² -C ¹⁸ salts of fatty acids (soaps), 4.4% non-ionic surfactants , 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamer, 2.0% glycerin, 0.08% preservatives, 1% ethanol, 1 , 6% enzyme mixture (protease, amylase, cellulase, manase), 0.009% cutinase, rest of demineralized water. The dosage of the liquid detergent is preferably between 4.5 and 6.0 grams per liter of wash liquor, for example 4.7, 4.9 or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.

The above-mentioned embodiments of the present invention include all solid, powder, liquid, gel or pasty dosage forms of agents according to the invention, which optionally may also consist of several phases and may be in compressed or compressed form. uncompressed. The agent may be in the form of a flowable powder, in particular with a bulk density of 300 g / l to 1200 g / l, in particular 500 g / l to 900 g / l or 600 g / l to 850 g / l. Solid dosage forms of the agent also include extrudates, granules, tablets, or bags. Alternatively, the agent may also be liquid, gel or pasty, for example in the form of a nonaqueous liquid detergent or a nonaqueous paste or in the form of an aqueous liquid detergent or a paste containing water. Furthermore, the agent may be in the form of a one-component system. Said means consist of a phase. Alternatively, a remedy can also consist of several phases. Therefore, such a medium is divided into several components.

Another object of the invention is a method for cleaning textiles, characterized in that a detergent according to the invention is used in at least one stage of the method.

In various embodiments, the method described above is characterized in that the detergent according to the invention is used at a temperature of 0-100 ° C, preferably 0-80 ° C, more preferably 30-70 ° C and most preferably at 40 -60 ° C.

This includes both manual and mechanical methods, with mechanical methods being preferred. Textile cleaning processes are generally characterized in that various active cleaning substances are applied to the items to be cleaned in various process steps and washed after the exposure time, or that the items to be cleaned are treated in some other way with a detergent or a solution or dilution of this agent. All conceivable washing or cleaning processes can be enriched in at least one of the process steps by using a detergent according to the invention and then represent embodiments of the present invention. All the facts, objects and embodiments that are described for the agents according to the present invention are also applicable to this object of the invention. For this reason, the disclosure is expressly referred to at this point at the appropriate point with the note that this disclosure also applies to the above processes according to the invention.

Since enzymes already naturally have a catalytic activity and also develop it in media that otherwise have no cleaning power, such as, for example, in mere buffers, a single and / or the only step of such a process may be that the The only active cleaning component is a cutinase that is contacted with the soil, preferably in a buffer solution or in water. This represents a further embodiment of this object of the invention.

Alternative embodiments of this object of the invention also represent processes for the treatment of textile raw materials or for the care of textiles, in which an agent according to the invention becomes active in at least one stage of the process. Among these, processes for textile raw materials, fibers or textiles with natural components are preferred, and very particularly for those with wool or silk.

Furthermore, the invention also covers the use of the agent described herein, for example as a detergent as described above, for the (improved) removal of soils, for example from textiles, in particular polyester textiles. Finally, the invention also relates to the use of a cutinase to reduce the pilling effects of an agent, preferably a detergent, particularly preferably a liquid detergent, the agent containing the cutinase. The cutinase can be a cutinase as defined herein. In various embodiments of use, the cutinase is in an amount of 0.00001-1% by weight, preferably in an amount of 0.0001-0.5% by weight, with particularly preferably, in an amount of 0.001-0.1% by weight, contained in the agent. In other different embodiments, the cutinase, which causes a reduction in the pilling effect, is applied to textiles, in particular to textiles that are made of polyester or comprise polyester.

All the facts, objects and embodiments that are described for the agents according to the invention and cutinase can also be applied to other subjects of the invention. Therefore, at this point, reference is expressly made to the disclosure at the appropriate point with the note that this disclosure also applies to the above method according to the invention and uses according to the invention.

Examples

Example 1: wash test

Detergent matrix used

This is a commercially available detergent matrix (without optical brighteners, perfume, or colorants) that was used for the wash test:

Dose 4.7 g / L

Wash test to determine the antipilling performance of enzymes.

In a commercially available washing machine, 20 identical tests are carried out one after another. Various polyesters and blended fabrics are used as fabrics to be evaluated, one of which is new and the other is pre-filled. After the 20 tests, the pilling reduction of the prepilled tissue and the pilling of the new tissue are visually evaluated.

Prepilled fabrics are produced by washing cycles repeated 20 times at 40 ° C in commercially available washing machines.

After each wash cycle, all clothes are tumble dried.

Washing conditions:

Water at 16 ° dH, 2.5 kg of clean laundry, normal program at 60 ° C, 46 g of detergent per machine as described above

Cutinase dose to be examined: 4 mg of active enzyme per washing machine

Claims (8)

1. Detergent, characterized in that it contains a cutinase, the cutinase being a cutinase that has at least 70% sequence identity with the amino acid sequence indicated in SEQ ID NO: 1 over its entire length. 2. Detergent according to claim 1, characterized in that (a) the cutinase can be obtained from a cutinase according to claim 1 as a starting molecule by one or more conservative amino acid substitutions; me (b) the cutinase can be obtained from a cutinase according to claim 1 as a starting molecule by fragmentation, deletion, insertion or substitution mutagenesis and comprises an amino acid sequence corresponding to the starting molecule in a length of at minus 210, 220, 230, 240, 250, 260, 270, 280 or 289 contiguous amino acids. The detergent according to one of claims 1 to 2, wherein the cutinase is contained in an amount of 0.00001-1% by weight, preferably in an amount of 0.0001-0.5% by weight, with particular preference, in an amount of 0.001-0.1% by weight in the detergent. Detergent according to one of claims 1 to 3, characterized in that (a) there is at least one additional ingredient selected from the group consisting of surfactants, conformers (enhancers), bleaches, bleach activators, water-miscible organic solvents, other enzymes, sequestering agents, electrolytes, pH regulators, optical brighteners, inhibitors graying, foam regulators, colorants and fragrances, as well as combinations thereof; and / or (b) is in solid or liquid form, preferably liquid. 5. Process for cleaning textiles, characterized in that a detergent according to one of claims 1 to 4 is used in at least one process step. 6. Use of a detergent according to one of claims 1 to 4 to remove dirt. 7. Use of a cutinase to reduce the pilling effects of a detergent, preferably a liquid detergent, wherein the detergent contains the cutinase, characterized in that the cutinase is a cutinase that has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length. 8. The use according to claim 7, wherein (a) the cutinase can be obtained from a cutinase according to claim 7 as a starting molecule by one or more conservative amino acid substitutions; me (b) the cutinase can be obtained from a cutinase according to claim 7 as a starting molecule by fragmentation, deletion, insertion or substitution mutagenesis and comprises an amino acid sequence corresponding to the original molecule in a length of at least 210, 220, 230, 240, 250, 260, 270, 280 or 289 contiguous amino acids; me (c) the cutinase is contained in an amount of 0.00001-1% by weight, preferably in an amount of 0.0001-0.5% by weight, particularly preferably, in an amount of 0.001-0.1% by weight in the detergent.