ES2712666T3 - Dispositivo - Google Patents
Dispositivo Download PDFInfo
- Publication number
- ES2712666T3 ES2712666T3 ES10778710T ES10778710T ES2712666T3 ES 2712666 T3 ES2712666 T3 ES 2712666T3 ES 10778710 T ES10778710 T ES 10778710T ES 10778710 T ES10778710 T ES 10778710T ES 2712666 T3 ES2712666 T3 ES 2712666T3
- Authority
- ES
- Spain
- Prior art keywords
- well
- diluent
- nucleic acid
- channel
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003321 amplification Effects 0.000 claims abstract description 83
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 83
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 75
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 72
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 72
- 239000012528 membrane Substances 0.000 claims abstract description 66
- 238000006243 chemical reaction Methods 0.000 claims abstract description 58
- 239000003085 diluting agent Substances 0.000 claims abstract description 58
- 238000003556 assay Methods 0.000 claims abstract description 13
- 238000012125 lateral flow test Methods 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims description 30
- 238000012360 testing method Methods 0.000 claims description 21
- 238000010521 absorption reaction Methods 0.000 claims description 18
- 238000000605 extraction Methods 0.000 claims description 9
- 238000001821 nucleic acid purification Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 74
- 238000001514 detection method Methods 0.000 description 21
- 238000010438 heat treatment Methods 0.000 description 18
- 238000000034 method Methods 0.000 description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 238000011109 contamination Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 239000011230 binding agent Substances 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 238000007397 LAMP assay Methods 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 239000002981 blocking agent Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 239000011324 bead Substances 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 239000004816 latex Substances 0.000 description 4
- 229920000126 latex Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000002787 reinforcement Effects 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 239000002250 absorbent Substances 0.000 description 3
- 230000002745 absorbent Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000012298 atmosphere Substances 0.000 description 3
- 230000006037 cell lysis Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- -1 polytetrafluoroethylene Polymers 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 229920001059 synthetic polymer Polymers 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000011901 isothermal amplification Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 240000006054 Agastache cana Species 0.000 description 1
- 229920002799 BoPET Polymers 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 238000006424 Flood reaction Methods 0.000 description 1
- 208000012860 Horse disease Diseases 0.000 description 1
- 235000010650 Hyssopus officinalis Nutrition 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241001148458 Taylorella equigenitalis Species 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000001745 anti-biotin effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 150000002194 fatty esters Chemical class 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004374 forensic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 229920001600 hydrophobic polymer Polymers 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 238000007826 nucleic acid assay Methods 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000010944 pre-mature reactiony Methods 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical class [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000006226 wash reagent Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0919159.4A GB0919159D0 (en) | 2009-11-02 | 2009-11-02 | Device and apparatus |
| PCT/GB2010/051832 WO2011051735A2 (en) | 2009-11-02 | 2010-11-02 | Device and apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2712666T3 true ES2712666T3 (es) | 2019-05-14 |
Family
ID=41435004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10778710T Active ES2712666T3 (es) | 2009-11-02 | 2010-11-02 | Dispositivo |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9163279B2 (https=) |
| EP (1) | EP2496351B1 (https=) |
| JP (1) | JP5934100B2 (https=) |
| CN (1) | CN102655938B (https=) |
| AU (1) | AU2010311138B2 (https=) |
| CA (1) | CA2778329C (https=) |
| ES (1) | ES2712666T3 (https=) |
| GB (1) | GB0919159D0 (https=) |
| TR (1) | TR201900390T4 (https=) |
| WO (1) | WO2011051735A2 (https=) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201014805D0 (en) | 2010-09-07 | 2010-10-20 | Multi Sense Technologies Ltd | Microfluidics based assay device |
| US9423398B2 (en) * | 2011-02-08 | 2016-08-23 | The Board Of Regents For Oklahoma State University | Apparatus and method for biologic sample rapid collection and recovery device, and convenient storage |
| US9724689B2 (en) * | 2012-11-20 | 2017-08-08 | Detectachem Llc | Colorimetric test system designed to control flow of simultaneously released chemicals to a target area |
| JP6272895B2 (ja) * | 2012-12-03 | 2018-01-31 | ザ セクレタリー オブ ステイト フォー エンバイロンメント，フード アンド ルーラル アフェアーズ | デバイスおよび装置 |
| EP2932268A4 (en) | 2012-12-17 | 2016-10-19 | Leukodx Ltd | SYSTEMS AND METHOD FOR RECOGNIZING A BIOLOGICAL CONDITION |
| US20140170678A1 (en) * | 2012-12-17 | 2014-06-19 | Leukodx Ltd. | Kits, compositions and methods for detecting a biological condition |
| CN105050720A (zh) | 2013-01-22 | 2015-11-11 | 华盛顿大学商业化中心 | 顺序递送流体体积和相关的设备、系统和方法 |
| US10946376B2 (en) | 2013-07-05 | 2021-03-16 | Thinxxs Microtechnology Ag | Carrier element for introducing a dry substance into a flow cell |
| DK2821138T4 (da) | 2013-07-05 | 2022-05-16 | Thinxxs Microtechnology Gmbh | Flydecelle med integreret tørstof |
| KR101398764B1 (ko) * | 2013-08-29 | 2014-05-27 | 강릉원주대학교산학협력단 | 입자의 이동에 의해 분석물질을 검출하는 장치 및 방법 |
| EP3060343A4 (en) * | 2013-10-22 | 2017-08-09 | Abon Biohparm (Hangzhou) Co., Ltd | A testing device for testing analytes in liquid samples |
| WO2015103326A1 (en) * | 2013-12-31 | 2015-07-09 | Canon U.S. Life Sciences, Inc. | Field deployable small format fast first result microfluidic system |
| CN106716116B (zh) * | 2014-09-23 | 2021-04-27 | 蒂尔实验室研究有限公司 | 用于微流体泪液收集和感兴趣的分析物的横向流动分析的集成的系统和方法 |
| FR3032719B1 (fr) * | 2015-02-13 | 2020-05-15 | Centre National De La Recherche Scientifique | Dispositif de diagnostic par amplification isotherme d'adn |
| SG11201708243RA (en) | 2015-04-07 | 2017-11-29 | Cell Id Pte Ltd | A dc heater |
| US10775370B2 (en) | 2015-07-17 | 2020-09-15 | Stat-Diagnostica & Innovation, S.L. | Fluidic system for performing assays |
| GB201611442D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Fluid control |
| WO2018022458A1 (en) * | 2016-07-25 | 2018-02-01 | Bio-Rad Laboratories, Inc. | Lateral flow device and method of use |
| GB201616718D0 (en) * | 2016-09-30 | 2016-11-16 | Microbiosensor Limited | Microbial sensing devices |
| GB201700340D0 (en) | 2017-01-09 | 2017-02-22 | Gsg Tech Ltd | fluid manipulation cartridge and controller mechanism |
| GB201715704D0 (en) * | 2017-09-28 | 2017-11-15 | Microbiosensor Ltd | Methods and devices |
| WO2019122788A1 (fr) * | 2017-12-21 | 2019-06-27 | Nanobiose | Puce micro-fluidique, laboratoire sur puce micro-fluidique, procédé de fabrication d'une telle puce et procédé d'analyse |
| FR3075817B1 (fr) * | 2017-12-21 | 2020-12-25 | Nanobiose | Puce micro-fluidique et laboratoire sur puce-micro-fluidique |
| FR3075823A1 (fr) * | 2017-12-21 | 2019-06-28 | Nanobiose | Procede d’analyse d’un echantillon cellulaire au moyen d’un laboratoire sur puce-micro-fluidique |
| US10046322B1 (en) * | 2018-03-22 | 2018-08-14 | Talis Biomedical Corporation | Reaction well for assay device |
| AU2019397371B2 (en) * | 2018-12-10 | 2025-09-18 | Combinati Incorporated | Microfluidic array for sample digitization |
| CN110358665A (zh) * | 2019-06-28 | 2019-10-22 | 马鞍山众慧惠生生物科技有限公司 | 箱式集成化核酸定量检测装置 |
| US11008627B2 (en) | 2019-08-15 | 2021-05-18 | Talis Biomedical Corporation | Diagnostic system |
| JP7164505B2 (ja) * | 2019-10-02 | 2022-11-01 | 積水化学工業株式会社 | マイクロ流路チップ |
| WO2021231607A1 (en) * | 2020-05-12 | 2021-11-18 | Godx, Inc. | Point of need diagnostic device and methods of use thereof |
| WO2021236850A1 (en) * | 2020-05-19 | 2021-11-25 | Mammoth Biosciences, Inc. | Devices, assays and methods for detection of nucleic acids |
| AU2021322453A1 (en) * | 2020-08-07 | 2023-03-09 | Go!Foton, Inc. | Nucleic acid extraction container and nucleic acid extraction method |
| US11567071B2 (en) | 2020-10-16 | 2023-01-31 | Fresenius Medical Care Holdings, Inc. | Devices and methods for lateral flow tests of bodily fluids |
| EP4173708A1 (de) * | 2021-10-28 | 2023-05-03 | thinXXS Microtechnology GmbH | Mikrofluidelement, insbesondere flusszelle, mit integriertem trockenreagenz |
| WO2023200703A1 (en) * | 2022-04-15 | 2023-10-19 | Siemens Healthcare Diagnostics Inc. | Creatinine lateral flow assay devices and methods of production and use thereof |
| WO2024127334A2 (en) * | 2022-12-16 | 2024-06-20 | 3M Innovative Properties Company | Sample collection and analysis system |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4857453A (en) * | 1987-04-07 | 1989-08-15 | Syntex (U.S.A.) Inc. | Immunoassay device |
| US6153425A (en) | 1995-07-13 | 2000-11-28 | Xtrana, Inc. | Self-contained device integrating nucleic acid extraction, amplification and detection |
| US20040110167A1 (en) | 1995-07-13 | 2004-06-10 | Gerdes John C. | Lateral flow system for nucleic acid detection |
| DK1179585T3 (da) * | 1997-12-24 | 2008-11-10 | Cepheid | Indretning og fremgangsmåde til lysis |
| AU2001243487A1 (en) | 2000-03-08 | 2001-09-17 | Imagene Technology, Inc | Lateral flow pcr with amplicon concentration and detection |
| US20060160078A1 (en) | 2002-07-12 | 2006-07-20 | Cardy Donald L N | Lateral flow assay device and method |
| CA2513880A1 (en) * | 2003-01-21 | 2004-08-05 | Micronics Inc. | Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing |
| CA2526409A1 (en) * | 2003-05-19 | 2004-12-02 | Brandeis University | Nucleic acid processing methods, kits and devices |
| WO2005083423A2 (en) | 2004-02-27 | 2005-09-09 | Board Of Regents, The University Of Texas System | System and method for integrating fluids and reagents in self-contained cartridges containing particle and membrane sensor elements |
| CA2562775C (en) | 2004-04-07 | 2015-08-04 | Access Bio, Inc. | Nucleic acid detection system |
| KR100705361B1 (ko) | 2004-08-21 | 2007-04-10 | 주식회사 엘지생명과학 | 모세관 유동 제어 모듈 및 이를 구비한 랩온어칩 |
| DK1843850T3 (en) | 2005-01-31 | 2015-12-07 | Realbio Technologies Ltd | LATERAL FLOW STEERING CAPILLAR DEVICE |
| KR20080027392A (ko) | 2005-07-14 | 2008-03-26 | 나노디텍 코포레이션 | 미세유체장치와 미세유체장치의 제작 및 사용방법 |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| AU2007217765A1 (en) * | 2006-02-21 | 2007-08-30 | Nanogen, Inc. | Methods and compositions for analyte detection |
| GB0604973D0 (en) | 2006-03-11 | 2006-04-19 | Central Science Lab Csl Of San | Purification method and kit |
| WO2008147382A1 (en) * | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Integrated microfluidic assay devices and methods |
| US8691592B2 (en) * | 2006-12-14 | 2014-04-08 | The Trustees Of The University Of Pennsylvania | Mechanically actuated diagnostic device |
| WO2008103824A1 (en) | 2007-02-23 | 2008-08-28 | Chinese Academy Of Inspection And Quarantine (Caiq) | Sensitivity-enhanced dot-antibody linked immunogold assay for virus detection |
| GB2453356A (en) | 2007-10-03 | 2009-04-08 | James Homrig | Assay device comprising control analytes to confirm assay completion |
| US8911938B2 (en) * | 2007-12-10 | 2014-12-16 | The Trustees Of The University Of Pennsylvania | Reaction chamber having pre-stored reagents |
| DE102007062441A1 (de) * | 2007-12-20 | 2009-06-25 | Aj Innuscreen Gmbh | Mobiles Schnelltestsystem für die Nukleinsäureanalytik |
| US8343443B2 (en) * | 2008-03-31 | 2013-01-01 | Agency For Science, Technology And Research | Fluid processing and transfer using inter-connected multi-chamber device |
| US8697007B2 (en) * | 2008-08-06 | 2014-04-15 | The Trustees Of The University Of Pennsylvania | Biodetection cassette with automated actuator |
-
2009
- 2009-11-02 GB GBGB0919159.4A patent/GB0919159D0/en not_active Ceased
-
2010
- 2010-11-02 EP EP10778710.3A patent/EP2496351B1/en active Active
- 2010-11-02 US US13/505,230 patent/US9163279B2/en active Active
- 2010-11-02 TR TR2019/00390T patent/TR201900390T4/tr unknown
- 2010-11-02 JP JP2012535940A patent/JP5934100B2/ja not_active Expired - Fee Related
- 2010-11-02 AU AU2010311138A patent/AU2010311138B2/en not_active Ceased
- 2010-11-02 WO PCT/GB2010/051832 patent/WO2011051735A2/en not_active Ceased
- 2010-11-02 CN CN201080049483.4A patent/CN102655938B/zh not_active Expired - Fee Related
- 2010-11-02 ES ES10778710T patent/ES2712666T3/es active Active
- 2010-11-02 CA CA2778329A patent/CA2778329C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011051735A3 (en) | 2011-08-18 |
| WO2011051735A2 (en) | 2011-05-05 |
| CN102655938B (zh) | 2014-09-17 |
| JP2013509173A (ja) | 2013-03-14 |
| EP2496351B1 (en) | 2019-01-02 |
| CA2778329C (en) | 2018-09-04 |
| CA2778329A1 (en) | 2011-05-05 |
| TR201900390T4 (tr) | 2019-02-21 |
| AU2010311138B2 (en) | 2014-08-28 |
| GB0919159D0 (en) | 2009-12-16 |
| JP5934100B2 (ja) | 2016-06-15 |
| EP2496351A2 (en) | 2012-09-12 |
| CN102655938A (zh) | 2012-09-05 |
| AU2010311138A1 (en) | 2012-05-03 |
| US9163279B2 (en) | 2015-10-20 |
| US20120270225A1 (en) | 2012-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2712666T3 (es) | Dispositivo | |
| US10620095B2 (en) | Apparatus and methods for detecting analytes | |
| JP6041448B2 (ja) | 試料調製デバイスおよび方法 | |
| US7871568B2 (en) | Rapid test apparatus | |
| US20230072999A1 (en) | Method and System for Sample Collection, Storage, Preparation and Detection | |
| US10006867B2 (en) | Device and apparatus | |
| US7794656B2 (en) | Device for handling and analysis of a biological sample | |
| US20050202568A1 (en) | Fluid sample analysis device with sealable sample storage reservoir | |
| US20080286831A1 (en) | Sample collector and tester | |
| CN107782575B (zh) | 一种收集和检测样本中被分析物质的系统及其方法 | |
| EP3495824A1 (en) | Analysis cell, analysis device, analysis equipment, and analysis system | |
| US20220219169A1 (en) | Device and method for detecting nucleic acids in biological samples | |
| ES2754237T3 (es) | Nuevo sistema de ensayo de PoC y procedimiento | |
| CN117242188A (zh) | 用于检测生物样品中的核酸的装置和方法 | |
| HK1198705B (en) | Apparatus and methods for detecting analytes |