ES2653727T3 - Desaturasas y proceso para la producción de ácidos grasos poliinsaturados en organismos transgénicos - Google Patents

Desaturasas y proceso para la producción de ácidos grasos poliinsaturados en organismos transgénicos Download PDF

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ES2653727T3
ES2653727T3 ES13180237.3T ES13180237T ES2653727T3 ES 2653727 T3 ES2653727 T3 ES 2653727T3 ES 13180237 T ES13180237 T ES 13180237T ES 2653727 T3 ES2653727 T3 ES 2653727T3
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acid sequence
nucleic acid
production
desaturases
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Toralf Senger
Jörg BAUER
Johnathan A. Napier
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BASF Plant Science GmbH
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
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    • C12P7/6481—Phosphoglycerides
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase

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Abstract

Un polinucleótido que comprende una secuencia de ácidos nucleicos seleccionada del grupo que consiste de: (a) una secuencia de ácidos nucleicos como se muestra en la SEQ ID NO. 50 o 51; (b) una secuencia de ácidos nucleicos que codifica un polipéptido que caracteriza una secuencia de aminoácidos como se muestra en cualquiera de la SEQ ID NO. 52; (c) una secuencia de ácidos nucleicos que codifica un polipéptido con una secuencia de aminoácidos con por lo menos 75 % de identidad con la SEQ ID NO. 52, y que codifica un polipéptido con una actividad de omega-3- desaturasa; y (d) una secuencia de ácidos nucleicos obtenible u obtenida mediante la amplificación de ácido nucleico del material genético de Hyaloperonospora parasitica usando el primer par - SEQ ID NO. 53 y SEQ ID NO. 55, o - SEQ ID NO. 54 y SEQ ID NO. 56 y que codifica un polipéptido con una actividad de omega-3-desaturasa.

Description

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Tabla 4A: Alimentación de levaduras
Nombre de la muestra/ carga de ácido graso
Etapa de reacción observada Índice de conversión (%) Actividad observada Fig.
Sustrato (% de contenido de ácido graso total)
Producto (% de contenido de ácido graso total) Esperado observado
Vector vacío pYES2 / 18: 2n-9
18:2n-6 25,3 18:3n-3 0,0 - - - 4a
d15Des (Ch)/18: 2n-9
18:2n-6 6,3 18:3n-3 5,7 > 0 47,5 ∆15-des. 4b
d15Des (Cy)/18: 2n-9 ω3Des (Hp)/18: 2n-9 ω3Des (Hp)/20: 3n-6 ω3Des (Hp)/20: 4n-6
18:2n-6 18:2n-6 20:3n-6 20:4n-6 13,0 12,6 6,3 5,7 18:3n-3 18:3n-3 20:4n-3 20:5n-3 0,9 0,0 0,6 1,8 > 0 > 0 > 0 > 0 6,4 0,0 8,6 24,0 ∆15-des. -ω3-Des ω3-Des 4c
A modo de control para el ensayo para la actividad ∆ 15-desaturasa, las levaduras se transforman con el vector vacío pYES, se carga el ácido graso 18:2 n-6 y el perfil de ácidos grasos se analiza (Figura 2A). En comparación, el 5 ácido graso adicional 18:3 n-3 se puede observar en levaduras que expresan las desaturasas d15Des (Ch) y d15Des(Cy) (Figura 2B, 2C). Estas desaturasas que se han ensayado, por lo tanto, tienen actividad ∆15-desaturasa.
Ejemplo 5: Producción de Plantas transgénicas para la producción de ácidos grasos poliinsaturados de cadena larga
Para producir ácidos grasos poliinsaturados de cadena larga en plantas, diversos genes de la ruta metabólica se combinan en un vector binario. Para producir el ácido graso ácido eicosapentaenoico (20:5∆5,8,11,14,17), se 10 combinan los genes como se describe en la Tabla 5. Análogamente, Los genes como se describe en la Tabla 6 se combinan para producir el ácido graso ácido docosahexaenoico (22:06∆4,7,10,13,16,19).
Tabla 5: Combinación de genes para la producción de ácido eicosapentaenoico
Gen
Actividad SEQ ID NO.
D6Des(Pir)
∆6-desaturasa 22
D6Elo(Pp)
∆6-elongasa 31
D5Des(Tc)
∆5-desaturasa 25
ω3-Des(Pi)
ω3-desaturasa 28
D15Des(Ch)
∆12-desaturasa 2
D15Des(Cy)
∆15-desaturasa 9
D12Des(Mac)
∆12-/∆15-desaturasa 16
ω 3Des(Hp)
ω3-desaturasa 51
35
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Claims (1)

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ES13180237.3T 2008-12-12 2009-12-08 Desaturasas y proceso para la producción de ácidos grasos poliinsaturados en organismos transgénicos Active ES2653727T3 (es)

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EP08171520 2008-12-12
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EP (2) EP2669380B1 (es)
AU (2) AU2009326129B2 (es)
CA (1) CA2744930C (es)
DE (1) DE112009003708T5 (es)
ES (2) ES2435572T3 (es)
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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011519552A (ja) 2008-04-25 2011-07-14 ビーエーエスエフ プラント サイエンス ゲーエムベーハー 植物種子油
US8809559B2 (en) 2008-11-18 2014-08-19 Commonwelath Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US9388436B2 (en) 2009-11-24 2016-07-12 Basf Plant Science Company Gmbh Fatty acid desaturase and uses thereof
US9347049B2 (en) 2009-11-24 2016-05-24 Basf Plant Science Company Gmbh Fatty acid elongase and uses thereof
BR122019026149A8 (pt) 2012-06-15 2022-06-28 Commw Scient Ind Res Org Célula hospedeira, processo de produzir um lipídio de planta extraído, método de produzir semente, farinha de semente, método para produzir um produto alimentício, uso e processo para produzir ésteres de etila
EP2970926B1 (en) * 2013-03-13 2018-01-31 DSM Nutritional Products AG Engineering microorganisms
PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
AU2015281791A1 (en) 2014-06-27 2017-01-12 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
WO2016075327A2 (en) 2014-11-14 2016-05-19 Basf Plant Science Company Gmbh Production of pufas in plants
US11690825B2 (en) 2016-03-09 2023-07-04 Board Of Regents, The University Of Texas System 20-HETE receptor (GPR75) antagonists and methods of use
EP3506881B1 (en) 2016-09-01 2025-10-22 Instituto De Biologia Molecular E Celular - IBMC Cyanobacterium extracellular polymer, compositions and uses thereof
CA3129494A1 (en) 2019-02-14 2020-08-20 Cargill, Incorporated Brassica plants producing elevated levels of polyunsaturated fatty acids
AU2021373697A1 (en) 2020-11-04 2023-06-08 Basf Plant Science Company Gmbh Harvest management
CN112592831A (zh) * 2021-01-06 2021-04-02 云南农业大学 一种快速分离三七圆斑病菌单个分生孢子的方法
EP4312517A1 (en) 2021-03-25 2024-02-07 BASF Plant Science Company GmbH Fertilizer management

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984002913A1 (en) 1983-01-17 1984-08-02 Monsanto Co Chimeric genes suitable for expression in plant cells
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
DK162399C (da) 1986-01-28 1992-03-23 Danisco Fremgangsmaade til ekspression af gener i baelgplanteceller, dna-fragment, rekombineret dna-fragment samt plasmid til brug ved udoevelsen af fremgangsmaaden
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US5110728A (en) 1986-07-31 1992-05-05 Calgene, Inc. Acyl carrier protein - DNA sequence and synthesis
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
NZ228320A (en) 1988-03-29 1991-06-25 Du Pont Nucleic acid promoter fragments of the promoter region homologous to the em gene of wheat, dna constructs therefrom and plants thereof
CA1341467C (en) 1988-07-29 2004-12-07 John C. Rogers Producing commercially valuable polypeptides with genetically transformed endosperm tissue
DE3843628A1 (de) 1988-12-21 1990-07-05 Inst Genbiologische Forschung Wundinduzierbare und kartoffelknollenspezifische transkriptionale regulation
EP0388186A1 (en) 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company External regulation of gene expression
ATE241007T1 (de) 1990-03-16 2003-06-15 Calgene Llc Dnas, die für pflanzliche desaturasen kodieren und deren anwendungen
ES2163392T3 (es) 1990-03-16 2002-02-01 Calgene Llc Nuevas secuencias expresadas preferentemente en el desarrollo de semillas precoces, y procedimientos relacionados con las mismas.
US5187267A (en) 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
WO1993020216A1 (en) 1991-02-22 1993-10-14 University Technologies International, Inc. Oil-body protein cis-elements as regulatory signals
ATE202144T1 (de) 1991-04-09 2001-06-15 Unilever Nv Pflanzen-promoter involviert in der kontrolle der lipid-biosynthese in samen
US5614393A (en) 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
PH31293A (en) 1991-10-10 1998-07-06 Rhone Poulenc Agrochimie Production of y-linolenic acid by a delta6-desaturage.
JPH07501701A (ja) * 1991-12-04 1995-02-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物由来の脂肪酸不飽和化酵素遺伝子
CA2084348A1 (en) 1991-12-31 1993-07-01 David F. Hildebrand Fatty acid alteration by a d9 desaturase in transgenic plant tissue
DE4208050A1 (de) 1992-03-13 1993-09-23 Bayer Ag Azolylmethyl-fluorcyclopropyl-derivate
CA2092069A1 (en) 1992-03-27 1993-09-28 Asako Iida An expression plasmid for seeds
AU3901993A (en) 1992-04-13 1993-11-18 Zeneca Limited Dna constructs and plants incorporating them
JPH0662870A (ja) 1992-08-18 1994-03-08 Mitsui Giyousai Shokubutsu Bio Kenkyusho:Kk 大豆ホスホエノールピルビン酸カルボキシラーゼ遺伝子のプロモーター領域及び５’非翻訳領域
DE69333025T2 (de) 1992-11-17 2004-04-29 E.I. Du Pont De Nemours And Co., Wilmington Für mikrosom delta 12 fettsäuredesaturase kodierende gene und verwandte enzyme von pflanzen
US7205457B1 (en) 1993-02-05 2007-04-17 Monsanto Technology Llc Altered linolenic and linoleic acid content in plants
GB9324707D0 (en) 1993-12-02 1994-01-19 Olsen Odd Arne Promoter
CN1048254C (zh) 1993-12-09 2000-01-12 托马斯杰弗逊大学 用于将预定的改变引入靶基因中的化合物
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
JP3313724B2 (ja) 1993-12-28 2002-08-12 麒麟麦酒株式会社 脂肪酸の不飽和化酸素遺伝子，当該遺伝子を含むベクター，当該遺伝子が導入された植物及びその作出方法
GB9403512D0 (en) 1994-02-24 1994-04-13 Olsen Odd Arne Promoter
US6310194B1 (en) 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
GB9421286D0 (en) 1994-10-21 1994-12-07 Danisco Promoter
US5689040A (en) 1995-02-23 1997-11-18 The Regents Of The University Of California Plant promoter sequences useful for gene expression in seeds and seedlings
CA2199158C (en) 1995-07-05 2011-01-25 Yukio Okada Seed-specific promoter from barley beta-amylase gene
ATE266093T1 (de) 1995-08-10 2004-05-15 Univ Rutgers Zellkern-codiertes transkriptionssystem plastiden höherer pflanzen
EP0880312B1 (en) 1995-12-14 2006-03-08 Cargill Incorporated Plants having mutant sequences that confer altered fatty acid profiles
EP0794250A1 (en) 1996-03-04 1997-09-10 Soremartec S.A. Isolation and sequencing of the hazel FAd2-N gene
US5731181A (en) 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
DE19626564A1 (de) 1996-07-03 1998-01-08 Hoechst Ag Genetische Transformation von Ciliatenzellen durch Microcarrier-Bombardement mit DNA beladenen Goldpartikeln
US5981841A (en) 1996-08-30 1999-11-09 Monsanto Company Early seed 5' regulatory sequence
US5977436A (en) 1997-04-09 1999-11-02 Rhone Poulenc Agrochimie Oleosin 5' regulatory region for the modification of plant seed lipid composition
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
AR013633A1 (es) 1997-04-11 2001-01-10 Calgene Llc METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
IL132148A0 (en) 1997-04-11 2001-03-19 Calgene Llc Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
CA2305628C (en) 1997-09-30 2008-08-26 The Regents Of The University Of California Production of proteins in plant seeds
GB9724783D0 (en) 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
BR9908624A (pt) 1998-03-11 2000-11-14 Novartis Ag Sequência de promotor de plastìdio de planta
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
US6004804A (en) 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
AU4564399A (en) 1998-06-12 1999-12-30 Abbott Laboratories Polyunsaturated fatty acids in plants
WO2000021557A1 (en) 1998-10-09 2000-04-20 Merck & Co., Inc. Delta 6 fatty acid desaturase
US6271360B1 (en) 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
EP1235842A4 (en) 1999-10-15 2003-04-23 Univ Massachusetts Rna interference pathway genes as tools for targeted genetic interference
DE10102338A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten
DE10102337A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
DE10219203A1 (de) 2002-04-29 2003-11-13 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in Pflanzen
AU2003296638B2 (en) 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
EP1682566B1 (en) * 2003-11-12 2013-06-05 E.I. Du Pont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
DE102005013779A1 (de) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
DE102006008030A1 (de) 2006-02-21 2007-08-23 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren
AR059376A1 (es) * 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
US7465793B2 (en) * 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
WO2008104559A1 (de) * 2007-02-27 2008-09-04 Norddeutsche Pflanzenzucht Verfahren zur herstellung von mehrfach ungesättigten fettsäuren in transgenen organismen

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