ES2540588T3 - Método para mejorar la eficiencia del uso de agua en plantas - Google Patents

Método para mejorar la eficiencia del uso de agua en plantas Download PDF

Info

Publication number
ES2540588T3
ES2540588T3 ES11155855.7T ES11155855T ES2540588T3 ES 2540588 T3 ES2540588 T3 ES 2540588T3 ES 11155855 T ES11155855 T ES 11155855T ES 2540588 T3 ES2540588 T3 ES 2540588T3
Authority
ES
Spain
Prior art keywords
plants
efficiency
improve
water use
transgenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11155855.7T
Other languages
English (en)
Inventor
Shimon Gepstein
Amira Gepstein
Eduardo Blumwald
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technion Research and Development Foundation Ltd
University of California
Original Assignee
Technion Research and Development Foundation Ltd
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technion Research and Development Foundation Ltd, University of California filed Critical Technion Research and Development Foundation Ltd
Application granted granted Critical
Publication of ES2540588T3 publication Critical patent/ES2540588T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método de aumento de la eficiencia de uso del agua por una planta, comprendiendo el método: (a) transformar una población de plantas con una casete de expresión recombinante que comprende un promotor SARK inducible por senescencia enlazado operativamente a una secuencia de ácido nucleico que codifica isopentenil-transferasa; y (b) seleccionar una planta que tiene una eficiencia de uso del agua incrementada, en donde el promotor SARK es (i) al menos 95% idéntico al promotor de SEQ ID NO: 1 o (ii) al menos 95% idéntico a 800 pb del extremo 5' de SEQ ID NO: 1.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
E11155855
24-06-2015
completo ambos linajes transgénicos (Figs. 2K y 2L). Las medidas del peso fresco de las plantas de tipo salvaje y transgénicas a finales del periodo de rehidratación demostraron que los linajes transgénicos alcanzaban un peso fresco de ~250 gramos/planta, mientras que el tipo salvaje seguía seco, con un peso que no excedía del 20% del correspondiente a los linajes transgénicos (Fig. 2). La Figura 3 muestra el peso fresco
5 de las plantas representadas en Fig. 2 después de 14 días de rehidratación. Los valores son la media ± SD (n = 40).
La expresión del gen IPT bajo la regulación del promotor del gen SARK confiere resistencia a la sequía a las
plantas transgénicas de Arabidopsis
Se cultivaron plantas de Arabidopsis thaliana bajo un régimen de día largo (16/8 horas) a 23 ºC. No pudieron
10 distinguirse diferencias morfológicas y de desarrollo de ningún tipo entre las plantas WT y las plantas transgénicas (pSARK:IPT) cultivadas en condiciones normales. En cambio, cuando se sometieron plantas de dos meses (en la etapa de floración avanzada) a estrés de sequía (sin adición alguna de agua a los tiestos) exhibían resistencia diferencial al estrés. Las plantas WT sufrieron marchitamiento severo irreversible y amarilleo de las hojas después de 12 días de deshidratación, mientras que 10 linajes independientes diferentes
15 de las plantas transgénicas T1 (pSARK:IPT) exhibían amarilleo moderado y se recuperaban del estrés de sequía después de 5 días de rehidratación (Fig. 4).
8
imagen7
imagen8

Claims (1)

  1. imagen1
ES11155855.7T 2005-03-21 2006-03-21 Método para mejorar la eficiencia del uso de agua en plantas Active ES2540588T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66403505P 2005-03-21 2005-03-21
US664035P 2005-03-21

Publications (1)

Publication Number Publication Date
ES2540588T3 true ES2540588T3 (es) 2015-07-10

Family

ID=37024661

Family Applications (2)

Application Number Title Priority Date Filing Date
ES11155855.7T Active ES2540588T3 (es) 2005-03-21 2006-03-21 Método para mejorar la eficiencia del uso de agua en plantas
ES06739467T Active ES2376924T3 (es) 2005-03-21 2006-03-21 Métodos para generar plantas resistentes a la sequ�?a.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES06739467T Active ES2376924T3 (es) 2005-03-21 2006-03-21 Métodos para generar plantas resistentes a la sequ�?a.

Country Status (13)

Country Link
US (1) US9624503B2 (es)
EP (2) EP2324703B1 (es)
CN (1) CN101146439B (es)
AT (1) ATE531807T1 (es)
AU (2) AU2006226863B2 (es)
BR (1) BRPI0607732B1 (es)
CA (1) CA2601605C (es)
ES (2) ES2540588T3 (es)
HU (1) HUE025741T2 (es)
MX (1) MX2007011612A (es)
PL (2) PL1863334T3 (es)
WO (1) WO2006102559A2 (es)
ZA (1) ZA200707905B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008068498A2 (en) * 2006-12-08 2008-06-12 The University Of York Regulation of plant metabolism
CA2804728A1 (en) 2010-07-15 2012-01-19 Technion Research & Development Foundation Ltd. Nucleic acid construct for increasing abiotic stress tolerance in plants
US10126760B2 (en) 2011-02-25 2018-11-13 Mks Instruments, Inc. System for and method of fast pulse gas delivery
US10031531B2 (en) 2011-02-25 2018-07-24 Mks Instruments, Inc. System for and method of multiple channel fast pulse gas delivery
WO2014101153A1 (zh) * 2012-12-31 2014-07-03 创世纪转基因技术有限公司 一种棉花异戊烯基转移酶ipt2及其编码基因与应用
WO2017083757A1 (en) 2015-11-13 2017-05-18 Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno Methods of engineered tissue succulence in plants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5763243A (en) 1994-12-08 1998-06-09 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5689042A (en) 1995-03-29 1997-11-18 Wisconsin Alumni Research Foundation Transgenic plants with altered senescence characteristics
WO1999029159A1 (en) 1997-12-08 1999-06-17 Vitality Biotechnologies, Inc. Plant senescence-associated genes
US20050086718A1 (en) * 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
AU2001259754A1 (en) * 2000-05-10 2001-11-20 Phycogen, Inc. Transgenic plants incorporating_genes of zostera marina
WO2003050287A2 (en) 2001-12-10 2003-06-19 Thomas Schmulling Method for modifying plant morphology, biochemistry and physiology comprising expression of plant cytokinin oxidase
AUPQ994600A0 (en) 2000-09-06 2000-09-28 Agriculture Victoria Services Pty Ltd Manipulation of plant senescene
EP1572927A4 (en) * 2002-04-08 2007-10-17 Pioneer Hi Bred Int REINFORCED EXTRACTION OF SILK UNDER STRESS
US7138566B2 (en) 2002-06-21 2006-11-21 Regents Of The University Of California Methods of modulating cytokinin related processes in a plant using B3 domain proteins

Also Published As

Publication number Publication date
HUE025741T2 (en) 2016-04-28
AU2011202138B2 (en) 2011-11-03
ATE531807T1 (de) 2011-11-15
CA2601605C (en) 2014-09-09
AU2006226863B2 (en) 2011-02-10
CN101146439B (zh) 2013-03-27
MX2007011612A (es) 2007-10-18
WO2006102559A3 (en) 2007-02-22
PL2324703T3 (pl) 2015-11-30
AU2006226863A1 (en) 2006-09-28
BRPI0607732A2 (pt) 2010-03-16
EP2324703B1 (en) 2015-05-06
US9624503B2 (en) 2017-04-18
EP2324703A1 (en) 2011-05-25
BRPI0607732B1 (pt) 2017-11-07
EP1863334A4 (en) 2008-11-26
ZA200707905B (en) 2008-12-31
AU2011202138A1 (en) 2011-05-26
PL1863334T3 (pl) 2012-04-30
US20080282365A1 (en) 2008-11-13
CA2601605A1 (en) 2006-09-28
WO2006102559A2 (en) 2006-09-28
ES2376924T3 (es) 2012-03-20
EP1863334A2 (en) 2007-12-12
CN101146439A (zh) 2008-03-19
EP1863334B1 (en) 2011-11-02

Similar Documents

Publication Publication Date Title
ES2540588T3 (es) Método para mejorar la eficiencia del uso de agua en plantas
Crespo et al. Can a rainbow consist of a single colour? A new comprehensive generic arrangement of the ‘Iris sensu latissimo’clade (Iridaceae), congruent with morphology and molecular data
BRPI0909953A2 (pt) Método para aumentar o rendimento no crescimento de uma planta sob condições que causam o estresse oxidativo periódico, método para aumentar a produção anual média de uma safra em uma área de produção, planta, parte de planta, uso de uma estufa para crescimento de plantas, método para otimizar a produção de um tipo específico de planta em uma área de cultivo selecionada e composição agrobiológica''
AR080975A1 (es) Polinucleotidos y polipeptidos aislados y metodos para utilizarlos para mejorar el rendimiento de la planta y/o las caracteristicas agricolas
BR122019015752B8 (pt) método para aumentar a tolerância ao estresse abiótico, o rendimento, a biomassa, taxa de crescimento e/ou vigor de uma planta, e, construto de ácido nucléico isolado
AR066451A1 (es) Combinaciones de moleculas de acido nucleico que codifican subunidades de piruvato quinasa y metodos que las utilizan en el mejoramiento de semillas
AR060435A1 (es) Polipeptidos relacionados con esteres y metodos para su uso en plantas
AR088897A1 (es) Plantas tolerantes a sequia
Matějka Disturbance-induced changes in the plant biomass in forests near Plešné and Čertovo Lakes.
UY36928A (es) Plantas modificadas genéticamente para mejorar el rendimiento de cultivos
Balapure et al. Avian diversity in Barna wetland of Narmada basin in central India
Jena Host plant specificity of Indian Tasar Silk Insect Antherae amylitta Drury (Saturniidae) during different seasons
Hruševar et al. Contribution to the knowledge of plant diversity of the planned Regional Park" Hrvatsko zagorje"
Ath-thar et al. Keragaan pertumbuhan hibridisasi empat strain ikan mas
Yumoto et al. Species composition of a middle altitude forest in Moukalaba-Doudou National Park, Gabon
Jasprica et al. Comparison of urban flora in Split, Dubrovnik, and Mostar
Czarna et al. New localities of Coleanthus subtilis (Tratt.) Seid.(Poaceae) in the" Milicz Ponds" Ornithological Reserve in Wielkopolska
Branson Further additions to the known spider fauna of Oklahoma
Žuna Pfeiffer et al. Comparative study of plant morphology and leaf anatomy of near threatened terrestrial species, Orchis morio L.
González Reyes et al. Development and reproduction of Raoiella indica Hirsts (Acari: Tenuipalpidae) in laboratory.
Chen et al. Effects of wild plants, cultivated season, and watering frequency on the antioxidant activity of Spiranthes sinensis (Pers.) Ames
Rathor et al. Couroupita guianensis Aubl.(Lecythidaceae) a recently introduced flowering plant of Marathwada.
Krasnevska et al. Genetic diversity of Dianthus arenarius assessed with retrotransposon-based molecular marker system (iPBS)
Fey et al. Interdimensional relations and yield of jatropha (Jatropha curcas L.) in silvopastoral system.
Sandev et al. Plant conservation Programs in the Botanical Garden of the Faculty of Science (Zagreb University, Croatia)