ES2527534A1 - Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. - Google Patents
Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. Download PDFInfo
- Publication number
- ES2527534A1 ES2527534A1 ES201230001A ES201230001A ES2527534A1 ES 2527534 A1 ES2527534 A1 ES 2527534A1 ES 201230001 A ES201230001 A ES 201230001A ES 201230001 A ES201230001 A ES 201230001A ES 2527534 A1 ES2527534 A1 ES 2527534A1
- Authority
- ES
- Spain
- Prior art keywords
- phosphate
- ion exchanger
- determining
- kit
- kit form
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 6
- 229910019142 PO4 Inorganic materials 0.000 title abstract 3
- -1 inositol phosphates Chemical class 0.000 title abstract 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 title 1
- 229960000367 inositol Drugs 0.000 title 1
- 235000021317 phosphate Nutrition 0.000 title 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 title 1
- INAPMGSXUVUWAF-GCVPSNMTSA-N [(2r,3s,5r,6r)-2,3,4,5,6-pentahydroxycyclohexyl] dihydrogen phosphate Chemical compound OC1[C@H](O)[C@@H](O)C(OP(O)(O)=O)[C@H](O)[C@@H]1O INAPMGSXUVUWAF-GCVPSNMTSA-N 0.000 abstract 3
- 150000002500 ions Chemical class 0.000 abstract 3
- 239000010452 phosphate Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 230000006978 adaptation Effects 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 230000003068 static effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. La presente invención se refiere a un procedimiento para la determinación de inositoles fosfato en muestras biológicas y que comprende las siguientes etapas: poner en contacto la muestra con un intercambiador iónico sólido usando una técnica estática de baño; aislar el intercambiador iónico sólido del resto de muestra sin reaccionar; extraer el inositol fosfato retenido sobre el intercambiador iónico; y detectar el inositol fosfato extraído. Además comprende el procedimiento de detección del inositol fosfato y un kit para llevar a cabo cualquiera de dichos procedimientos.
Description
Claims (1)
-
imagen1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201230001A ES2527534B1 (es) | 2012-01-02 | 2012-01-02 | Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. |
| PCT/ES2012/070873 WO2013102688A1 (es) | 2012-01-02 | 2012-12-18 | Procedimiento para la determinación de inositoles fosfato y su adaptación como kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201230001A ES2527534B1 (es) | 2012-01-02 | 2012-01-02 | Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2527534A1 true ES2527534A1 (es) | 2015-01-26 |
| ES2527534B1 ES2527534B1 (es) | 2017-01-17 |
Family
ID=48744994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201230001A Active ES2527534B1 (es) | 2012-01-02 | 2012-01-02 | Procedimiento para la determinación de inositoles fosfato y su adaptación como kit. |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2527534B1 (es) |
| WO (1) | WO2013102688A1 (es) |
-
2012
- 2012-01-02 ES ES201230001A patent/ES2527534B1/es active Active
- 2012-12-18 WO PCT/ES2012/070873 patent/WO2013102688A1/es not_active Ceased
Non-Patent Citations (3)
| Title |
|---|
| BARTLETT G. R. ¿Isolation and assay of red-cell inositol polyphosphates.¿ Analytical Biochemiestry (1982) Vol. 124, pages 425-431.Resumen, página 425-427. * |
| GRASES F., MARCH J.G., PRIETO R. M., SIMONET B. M., COSTA-BAUZÁ A., GARCÍA-RAJA A., and CONTE A. ¿Urinary phytate in calcium oxalate Stone formers and healthy people.¿ Scandinavian Journal of Urology and Nephrology (2000) Vol. 34, pages 162-164. Todo el documento. * |
| WANG N., HATCHER D. W., TYLER R. T., TOEWS R., GAWALKO E. J. ¿Effect of cooking on the composition of beans (Phaseolus vulgaris L.) and chickpeas (Cicer arietinum L.) Food Research International (2010) Vol. 43, pages 589-594.Página 590, columna 2. * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013102688A1 (es) | 2013-07-11 |
| ES2527534B1 (es) | 2017-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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