ES2522765A1 - Método para dectectar inserciones de espaciadores en estructuras CRISPR - Google Patents
Método para dectectar inserciones de espaciadores en estructuras CRISPR Download PDFInfo
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- ES2522765A1 ES2522765A1 ES201300203A ES201300203A ES2522765A1 ES 2522765 A1 ES2522765 A1 ES 2522765A1 ES 201300203 A ES201300203 A ES 201300203A ES 201300203 A ES201300203 A ES 201300203A ES 2522765 A1 ES2522765 A1 ES 2522765A1
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- spacers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
La presente invención se refiere a un método para detectar inserciones de espaciadores mediante selección independiente de la interferencia a partir de estructuras artificiales basadas en repeticiones palindrómicas cortas agrupadas regularmente espaciadas (CRISPR) que comprende la inserción de al menos una unidad CRISPR-espaciador, en la estructura artificial dentro de la secuencia que codifica un gen testigo, que restaura la pauta de lectura de traducción, donde la expresión del gen testigo es indicativo de la inserción del espaciador. También se refiera a la estructura artificial utilizada.
Description
Claims (1)
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imagen1 imagen2
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300203A ES2522765B2 (es) | 2013-02-22 | 2013-02-22 | Método para dectectar inserciones de espaciadores en estructuras CRISPR |
PCT/ES2014/070093 WO2014128324A1 (es) | 2013-02-22 | 2014-02-11 | Método para detectar inserciones de espaciadores en estructuras crispr |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300203A ES2522765B2 (es) | 2013-02-22 | 2013-02-22 | Método para dectectar inserciones de espaciadores en estructuras CRISPR |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2522765A1 true ES2522765A1 (es) | 2014-11-17 |
ES2522765B2 ES2522765B2 (es) | 2015-03-18 |
Family
ID=51390532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201300203A Active ES2522765B2 (es) | 2013-02-22 | 2013-02-22 | Método para dectectar inserciones de espaciadores en estructuras CRISPR |
Country Status (2)
Country | Link |
---|---|
ES (1) | ES2522765B2 (es) |
WO (1) | WO2014128324A1 (es) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9834791B2 (en) | 2013-11-07 | 2017-12-05 | Editas Medicine, Inc. | CRISPR-related methods and compositions with governing gRNAS |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
CN104504304B (zh) * | 2014-11-03 | 2017-08-25 | 深圳先进技术研究院 | 一种成簇的规律间隔的短回文重复序列识别方法及装置 |
CN108513575A (zh) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | 核碱基编辑器及其用途 |
WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
SG11201908658TA (en) | 2017-03-23 | 2019-10-30 | Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
EP4146804A1 (en) | 2020-05-08 | 2023-03-15 | The Broad Institute Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2341149A1 (en) * | 2005-08-26 | 2011-07-06 | Danisco A/S | Use of CRISPR associated genes (CAS) |
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2013
- 2013-02-22 ES ES201300203A patent/ES2522765B2/es active Active
-
2014
- 2014-02-11 WO PCT/ES2014/070093 patent/WO2014128324A1/es active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2341149A1 (en) * | 2005-08-26 | 2011-07-06 | Danisco A/S | Use of CRISPR associated genes (CAS) |
Non-Patent Citations (6)
Title |
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ALMENDROS, C. et al., `Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli¿, PLOS ONE, 2012, No.7, No.11, Página e50797, ISSN: 1932-6203 (electronic), doi: 10.1371/journal.pone.0050797, Epub: 26/11/2012, todo el documento. * |
DÍEZ-VILLASEÑOR, C. et al., `Diversity of CRISPR loci in Escherichia coli¿, MICROBIOLOGY, 2010, Vol. 156, No. Pt 5, Páginas 1351-1361, ISSN: 1350-0872, doi: 10.1099/mic.0.036046-0, todo el documento. * |
MOJICA, F.J. et al., `Short motif sequences determine the targets of the prokaryotic CRISPR defence system¿, MICROBIOLOGY, 2009, Vol. 155, No. Pt 3, Páginas 733-740, ISSN: 1350-0872 (Print), doi: 10.1099/mic.0.023960-0, todo el documento. * |
POUGACH, K. et al., `Transcription, processing and function of CRISPR cassettes in Escherichia coli¿, MOLECULAR MICROBIOLOGY, 2010, Vol. 77, No. 6, Páginas 1367-1379, ISSN. 0950-382X (print), ISSN: 1365-2958 (electronic), doi: 10.1111/j.1365-2958.2010.07265.x, todo el documento. * |
SEMENOVA, E. et al., `Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence¿, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, 2011, Vol.108, No. 25, Páginas 10098-10103, ISSN: 0027-8424 (print), ISSN: 1091-6490 (electronic), doi: 10.1073/pnas.1104144108, todo el documento. * |
SWARTS, D.C. et al., `CRISPR interference directs strand specific spacer acquisition¿, PLOS ONE, 2012, Vol.7, No. 4, Página e35888, ISSN: 1932-6203(print), ISSN: 1932-6203(electronic), doi: 10.1371/journal.pone.0035888, Epub: 27/04/2012, todo el documento. * |
Also Published As
Publication number | Publication date |
---|---|
WO2014128324A1 (es) | 2014-08-28 |
ES2522765B2 (es) | 2015-03-18 |
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