ES2421459A1 - Cebadores especiales para la reaccion en cadena de la polimerasa - Google Patents

Cebadores especiales para la reaccion en cadena de la polimerasa

Info

Publication number
ES2421459A1
ES2421459A1 ES201230120A ES201230120A ES2421459A1 ES 2421459 A1 ES2421459 A1 ES 2421459A1 ES 201230120 A ES201230120 A ES 201230120A ES 201230120 A ES201230120 A ES 201230120A ES 2421459 A1 ES2421459 A1 ES 2421459A1
Authority
ES
Spain
Prior art keywords
chain reaction
polymerase chain
primers
reaction primers
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201230120A
Other languages
English (en)
Other versions
ES2421459B1 (es
Inventor
Ciara O'sullivan
Ioanis Katakis
Valerio Beni
Andreas Willems
Rainer Frank
Thomas Hansen-Hagge
Hamdi Abdelazim Osman Joda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INNOTRAIN DIAGNOSTIK GmbH
Institut fuer Mikrotechnik Mainz GmbH
Institucio Catalana de Recerca i Estudis Avancats ICREA
Universitat Rovira i Virgili URV
Original Assignee
INNOTRAIN DIAGNOSTIK GmbH
Institut fuer Mikrotechnik Mainz GmbH
Institucio Catalana de Recerca i Estudis Avancats ICREA
Universitat Rovira i Virgili URV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOTRAIN DIAGNOSTIK GmbH, Institut fuer Mikrotechnik Mainz GmbH, Institucio Catalana de Recerca i Estudis Avancats ICREA, Universitat Rovira i Virgili URV filed Critical INNOTRAIN DIAGNOSTIK GmbH
Priority to ES201230120A priority Critical patent/ES2421459B1/es
Priority to PCT/ES2013/070045 priority patent/WO2013113965A1/es
Publication of ES2421459A1 publication Critical patent/ES2421459A1/es
Application granted granted Critical
Publication of ES2421459B1 publication Critical patent/ES2421459B1/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cebadores especiales para la reacción en cadena de la polimerasa. La presente invención pertenece al campo de las técnicas de biología molecular, en particular al campo de la técnica de la reacción en cadena de la polimerasa (PCR). Más específicamente, se refiere a cebadores diseñados especialmente para la PCR y a la detección directa de una secuencia diana. Esto se consigue debido a su diseño especial y, más específicamente, a la presencia en el producto de amplificación obtenido, con el uso de dichos cebadores, de colas de cadena sencilla. Estas colas permiten la captura del producto de amplificación a un sustrato funcionalizado con sondas de captura y/o su detección sencilla con sondas informadoras.
ES201230120A 2012-01-30 2012-01-30 Cebadores especiales para la reacción en cadena de la polimerasa Expired - Fee Related ES2421459B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201230120A ES2421459B1 (es) 2012-01-30 2012-01-30 Cebadores especiales para la reacción en cadena de la polimerasa
PCT/ES2013/070045 WO2013113965A1 (es) 2012-01-30 2013-01-30 Cebadores especiales para la reacción en cadena de la polimerasa

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201230120A ES2421459B1 (es) 2012-01-30 2012-01-30 Cebadores especiales para la reacción en cadena de la polimerasa

Publications (2)

Publication Number Publication Date
ES2421459A1 true ES2421459A1 (es) 2013-09-02
ES2421459B1 ES2421459B1 (es) 2015-01-22

Family

ID=48904446

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201230120A Expired - Fee Related ES2421459B1 (es) 2012-01-30 2012-01-30 Cebadores especiales para la reacción en cadena de la polimerasa

Country Status (2)

Country Link
ES (1) ES2421459B1 (es)
WO (1) WO2013113965A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021224359A1 (en) * 2020-05-08 2021-11-11 F. Hoffmann-La Roche Ag Removal of excess oligonucleotides from a reaction mixture

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180237824A1 (en) * 2014-11-10 2018-08-23 Gna Biosolutions Gmbh Method for multiplying nucleic acids
WO2018005284A1 (en) * 2016-06-27 2018-01-04 The United State Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and compositions for influenza a virus subtyping

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998014610A2 (en) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
WO2003093509A1 (en) * 2002-05-01 2003-11-13 Seegene, Inc. Methods and compositions for improving specificity of pcr amplication
US20040038194A1 (en) * 2000-10-06 2004-02-26 Hofmann Scott Daniel Diagnostic polymerase chain reaction process utilizing simultaneous capture and detection of amplicons
WO2006095550A1 (ja) * 2005-03-04 2006-09-14 Kyoto University Ｐｃｒプライマー、それを利用したｐｃｒ法及びｐｃｒ増幅産物、並びにｐｃｒ増幅産物を利用するデバイス及びｄｎａ-タンパク複合体

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998014610A2 (en) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US20040038194A1 (en) * 2000-10-06 2004-02-26 Hofmann Scott Daniel Diagnostic polymerase chain reaction process utilizing simultaneous capture and detection of amplicons
WO2003093509A1 (en) * 2002-05-01 2003-11-13 Seegene, Inc. Methods and compositions for improving specificity of pcr amplication
WO2006095550A1 (ja) * 2005-03-04 2006-09-14 Kyoto University Ｐｃｒプライマー、それを利用したｐｃｒ法及びｐｃｒ増幅産物、並びにｐｃｒ増幅産物を利用するデバイス及びｄｎａ-タンパク複合体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Application of L-DNA as a molecular tag, Hayashi, G. et al; 09/2005; Nucleic Acids Symp Ser. Vol 49 (1); págs. 261-262; doi: 10.1093/nass/49.1.261figura 1, párrafo [1]; *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021224359A1 (en) * 2020-05-08 2021-11-11 F. Hoffmann-La Roche Ag Removal of excess oligonucleotides from a reaction mixture

Also Published As

Publication number Publication date
ES2421459B1 (es) 2015-01-22
WO2013113965A1 (es) 2013-08-08

Similar Documents

Publication Publication Date Title
WO2015123430A3 (en) Single molecule electronic multiplex snp assay and pcr analysis
MX367666B (es) Composiciones y métodos para cuantificar una secuencia de ácido nucleico en una muestra.
MX2017005159A (es) Composiciones y metodos para detectar un virus de rna.
EP4303314A3 (en) Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection
MX2017001405A (es) Deteccion de acidos nucleicos diana mediante el uso de hibridacion.
WO2015081229A3 (en) Selective amplification of nucleic acid sequences
EP3115457A4 (en) Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
EP2746395A4 (en) PRIMER SET, METHOD FOR AMPLIFYING TARGET NUCLEIC ACID SEQUENCE USING THE SAME, AND METHOD FOR DETECTING NUCLEIC ACID MUTRED USING THE SAME
WO2012095639A3 (en) Methods, compositions, and kits for determing the presence/absence of a varian nucleic acid sequence
IN2012DN06299A (es)
WO2012151560A3 (en) Methods for detecting nucleic acid sequence variants
WO2015114469A3 (en) Covered sequence conversion dna and detection methods
EP3056567A4 (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
WO2014005125A3 (en) Fluorescent compounds and uses thereof
WO2014005969A9 (fr) Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees
EA038921B9 (ru) Обнаружение целевой нуклеиновой кислоты и ее вариантов
WO2012177695A3 (en) Hybrid polymerases having the ability to produce long amplicons
MX2017000391A (es) Tecnologia de amplificacion de adn.
GB201017978D0 (en) Multiplex amplification and detection
WO2016059473A3 (en) Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same
WO2012021493A3 (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
MX2020002826A (es) Detección de la amplificación de la recombinasa polimerasa usando la sonda de hapteno doble.
EP4282961A3 (en) Compositions and methods for the detection and analysis of mycobacterium tuberculosis
MX2016013877A (es) Composiciones y metodos para mejorar y/o predecir la amplificacion de adn.
ES2421459A1 (es) Cebadores especiales para la reaccion en cadena de la polimerasa

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 2421459

Country of ref document: ES

Kind code of ref document: B1

Effective date: 20150122

FD2A Announcement of lapse in spain

Effective date: 20210915