ES2386414T8 - Procedimiento para la desparafinación de muestras biológicas - Google Patents

Procedimiento para la desparafinación de muestras biológicas Download PDF

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Publication number
ES2386414T8
ES2386414T8 ES10165393.9T ES10165393T ES2386414T8 ES 2386414 T8 ES2386414 T8 ES 2386414T8 ES 10165393 T ES10165393 T ES 10165393T ES 2386414 T8 ES2386414 T8 ES 2386414T8
Authority
ES
Spain
Prior art keywords
dewaxing
procedure
biological samples
biological
samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10165393.9T
Other languages
English (en)
Other versions
ES2386414T3 (es
Inventor
Christoph Kirsch
Claudia Beyard
Markus Meusel
Edmund Radmacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Axagarius GmbH and Co KG
Original Assignee
Axagarius GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axagarius GmbH and Co KG filed Critical Axagarius GmbH and Co KG
Application granted granted Critical
Publication of ES2386414T3 publication Critical patent/ES2386414T3/es
Publication of ES2386414T8 publication Critical patent/ES2386414T8/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • AHUMAN NECESSITIES
    • A47FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47GHOUSEHOLD OR TABLE EQUIPMENT
    • A47G1/00Mirrors; Picture frames or the like, e.g. provided with heating, lighting or ventilating means
    • A47G1/02Mirrors used as equipment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00Optical elements other than lenses
    • G02B5/08Mirrors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES10165393T 2009-07-01 2010-06-09 Procedimiento para la desparafinación de muestras biológicas Active ES2386414T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009031434.2A DE102009031434B4 (de) 2009-07-01 2009-07-01 Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit hierfür
DE102009031434 2009-07-01

Publications (2)

Publication Number Publication Date
ES2386414T3 ES2386414T3 (es) 2012-08-20
ES2386414T8 true ES2386414T8 (es) 2018-07-16

Family

ID=42668068

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10165393T Active ES2386414T3 (es) 2009-07-01 2010-06-09 Procedimiento para la desparafinación de muestras biológicas

Country Status (4)

Country Link
US (1) US9845488B2 (es)
EP (1) EP2270151B1 (es)
DE (2) DE102009031434B4 (es)
ES (1) ES2386414T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法
EP2556158B1 (en) 2010-04-08 2019-12-18 Qiagen GmbH Chromatographic device and method for isolating and purifying nucleic acids
EP2395082A1 (en) 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
EP2686662B1 (en) * 2011-02-28 2022-01-19 Agilent Technologies, Inc. Two phase immiscible system for the pretreatment of embedded biological samples
US8852919B2 (en) * 2011-11-17 2014-10-07 Rheonix, Inc. Microfluidic apparatus, method, and applications
JP6060567B2 (ja) * 2012-08-30 2017-01-18 凸版印刷株式会社 包埋組織からの核酸抽出方法
EP2730653A1 (en) * 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
DE102014118532B4 (de) 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
KR101728096B1 (ko) * 2015-03-12 2017-04-20 재단법인 아산사회복지재단 Ffpe 조직에서 핵산의 분리 방법
WO2017184984A1 (en) * 2016-04-21 2017-10-26 Cell Data Sciences, Inc. Biomolecule processing from fixed biological samples
US10274405B2 (en) 2016-11-25 2019-04-30 Exscale Biospecimen Solutions Ab Deparaffinization of FFPE tissue samples
CN113755486A (zh) * 2021-09-23 2021-12-07 翌圣生物科技(上海)股份有限公司 用于处理石蜡包埋样本的脱蜡剂及从石蜡包埋样本中提取核酸的方法
WO2024194332A1 (en) 2023-03-20 2024-09-26 Bioecho Life Sciences Gmbh Method for isolating nucleic acid from a biological sample

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
SE9603599D0 (sv) 1996-10-02 1996-10-02 Pharmacia Biotech Ab A method of separating a two phase system
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
WO2002023156A1 (en) 2000-09-15 2002-03-21 Biogenex Laboratories Enhancement of in situ hybridization
JP4579234B2 (ja) 2003-03-10 2010-11-10 エクスプレッション、パソロジー、インコーポレイテッド 組織病理学的に加工処理された生物サンプル、組織および細胞からの液体組織調製物
US8679745B2 (en) 2004-09-30 2014-03-25 Epigenomics Ag Method for providing DNA fragments derived from an archived sample
CA2591868A1 (en) * 2004-12-30 2006-07-13 Ventana Medical Systems, Inc. Low temperature deparaffinization
US9243241B2 (en) 2005-05-31 2016-01-26 Life Technologies Corporation Separation and purification of nucleic acid from paraffin-containing samples
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
EP1793218A1 (en) * 2005-11-30 2007-06-06 Dako Denmark A/S Removal of embedding medium
DE102005060738A1 (de) * 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量

Also Published As

Publication number Publication date
DE102009031434B4 (de) 2015-03-05
EP2270151B1 (de) 2012-07-04
DE202009018981U1 (de) 2015-03-11
EP2270151A1 (de) 2011-01-05
US9845488B2 (en) 2017-12-19
DE102009031434A1 (de) 2011-01-05
ES2386414T3 (es) 2012-08-20
US20110003369A1 (en) 2011-01-06

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