ES2386414T8 - Procedimiento para la desparafinación de muestras biológicas - Google Patents
Procedimiento para la desparafinación de muestras biológicas Download PDFInfo
- Publication number
- ES2386414T8 ES2386414T8 ES10165393.9T ES10165393T ES2386414T8 ES 2386414 T8 ES2386414 T8 ES 2386414T8 ES 10165393 T ES10165393 T ES 10165393T ES 2386414 T8 ES2386414 T8 ES 2386414T8
- Authority
- ES
- Spain
- Prior art keywords
- dewaxing
- procedure
- biological samples
- biological
- samples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012472 biological sample Substances 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47G—HOUSEHOLD OR TABLE EQUIPMENT
- A47G1/00—Mirrors; Picture frames or the like, e.g. provided with heating, lighting or ventilating means
- A47G1/02—Mirrors used as equipment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/08—Mirrors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102009031434.2A DE102009031434B4 (de) | 2009-07-01 | 2009-07-01 | Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit hierfür |
DE102009031434 | 2009-07-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2386414T3 ES2386414T3 (es) | 2012-08-20 |
ES2386414T8 true ES2386414T8 (es) | 2018-07-16 |
Family
ID=42668068
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES10165393T Active ES2386414T3 (es) | 2009-07-01 | 2010-06-09 | Procedimiento para la desparafinación de muestras biológicas |
Country Status (4)
Country | Link |
---|---|
US (1) | US9845488B2 (es) |
EP (1) | EP2270151B1 (es) |
DE (2) | DE102009031434B4 (es) |
ES (1) | ES2386414T3 (es) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102812118B (zh) | 2010-04-08 | 2016-01-20 | 恰根有限公司 | 分离和纯化核酸的方法 |
EP2556158B1 (en) | 2010-04-08 | 2019-12-18 | Qiagen GmbH | Chromatographic device and method for isolating and purifying nucleic acids |
EP2395082A1 (en) | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
EP2686662B1 (en) * | 2011-02-28 | 2022-01-19 | Agilent Technologies, Inc. | Two phase immiscible system for the pretreatment of embedded biological samples |
US8852919B2 (en) * | 2011-11-17 | 2014-10-07 | Rheonix, Inc. | Microfluidic apparatus, method, and applications |
JP6060567B2 (ja) * | 2012-08-30 | 2017-01-18 | 凸版印刷株式会社 | 包埋組織からの核酸抽出方法 |
EP2730653A1 (en) * | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
DE102014118532B4 (de) | 2014-12-12 | 2017-03-23 | Axagarius Gmbh & Co. Kg | Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben |
KR101728096B1 (ko) * | 2015-03-12 | 2017-04-20 | 재단법인 아산사회복지재단 | Ffpe 조직에서 핵산의 분리 방법 |
WO2017184984A1 (en) * | 2016-04-21 | 2017-10-26 | Cell Data Sciences, Inc. | Biomolecule processing from fixed biological samples |
US10274405B2 (en) | 2016-11-25 | 2019-04-30 | Exscale Biospecimen Solutions Ab | Deparaffinization of FFPE tissue samples |
CN113755486A (zh) * | 2021-09-23 | 2021-12-07 | 翌圣生物科技(上海)股份有限公司 | 用于处理石蜡包埋样本的脱蜡剂及从石蜡包埋样本中提取核酸的方法 |
WO2024194332A1 (en) | 2023-03-20 | 2024-09-26 | Bioecho Life Sciences Gmbh | Method for isolating nucleic acid from a biological sample |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
SE9603599D0 (sv) | 1996-10-02 | 1996-10-02 | Pharmacia Biotech Ab | A method of separating a two phase system |
US6248535B1 (en) * | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
WO2002023156A1 (en) | 2000-09-15 | 2002-03-21 | Biogenex Laboratories | Enhancement of in situ hybridization |
JP4579234B2 (ja) | 2003-03-10 | 2010-11-10 | エクスプレッション、パソロジー、インコーポレイテッド | 組織病理学的に加工処理された生物サンプル、組織および細胞からの液体組織調製物 |
US8679745B2 (en) | 2004-09-30 | 2014-03-25 | Epigenomics Ag | Method for providing DNA fragments derived from an archived sample |
CA2591868A1 (en) * | 2004-12-30 | 2006-07-13 | Ventana Medical Systems, Inc. | Low temperature deparaffinization |
US9243241B2 (en) | 2005-05-31 | 2016-01-26 | Life Technologies Corporation | Separation and purification of nucleic acid from paraffin-containing samples |
US7544471B2 (en) * | 2005-07-30 | 2009-06-09 | Agilent Technologies, Inc. | Preparing RNA from a wax-embedded tissue specimen |
EP1793218A1 (en) * | 2005-11-30 | 2007-06-06 | Dako Denmark A/S | Removal of embedding medium |
DE102005060738A1 (de) * | 2005-12-16 | 2007-06-21 | Qiagen Gmbh | Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben |
JP2008043332A (ja) * | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
-
2009
- 2009-07-01 DE DE102009031434.2A patent/DE102009031434B4/de active Active
- 2009-07-01 DE DE202009018981.3U patent/DE202009018981U1/de not_active Expired - Lifetime
-
2010
- 2010-06-09 ES ES10165393T patent/ES2386414T3/es active Active
- 2010-06-09 EP EP10165393A patent/EP2270151B1/de active Active
- 2010-06-30 US US12/827,210 patent/US9845488B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
DE102009031434B4 (de) | 2015-03-05 |
EP2270151B1 (de) | 2012-07-04 |
DE202009018981U1 (de) | 2015-03-11 |
EP2270151A1 (de) | 2011-01-05 |
US9845488B2 (en) | 2017-12-19 |
DE102009031434A1 (de) | 2011-01-05 |
ES2386414T3 (es) | 2012-08-20 |
US20110003369A1 (en) | 2011-01-06 |
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