ES2386264A1 - Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0 - Google Patents

Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0 Download PDF

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Publication number
ES2386264A1
ES2386264A1 ES201031864A ES201031864A ES2386264A1 ES 2386264 A1 ES2386264 A1 ES 2386264A1 ES 201031864 A ES201031864 A ES 201031864A ES 201031864 A ES201031864 A ES 201031864A ES 2386264 A1 ES2386264 A1 ES 2386264A1
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ES
Spain
Prior art keywords
reverse transcriptases
group
immunodeficiency virus
human immunodeficiency
virus type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201031864A
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English (en)
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ES2386264B1 (es
Inventor
Luis Menéndez Arias
Verónica Barrioluengo Fernández
Maria Del Mar Álvarez García
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
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Consejo Superior de Investigaciones Cientificas CSIC
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Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES201031864A priority Critical patent/ES2386264B1/es
Priority to PCT/ES2011/070801 priority patent/WO2012080541A1/es
Publication of ES2386264A1 publication Critical patent/ES2386264A1/es
Application granted granted Critical
Publication of ES2386264B1 publication Critical patent/ES2386264B1/es
Withdrawn - After Issue legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo O.La presente invención se refiere a retrotranscriptasas aisladas de un virus de la inmunodeficiencia humana de tipo 1 (VIH-1) de grupo O y modificadas en la posición 65 y/o en la posición 75, que presentan una elevada fidelidad de copia y termoestabilidad, así como a su uso para llevar a cabo la retrotranscripción, amplificación o secuenciación de un ácido nucleico molde. La invención se refiere, además, a un método para obtener dichas retrotranscriptasas. También se refiere a un método de retrotranscripción, a un método de amplificación, a un método de secuenciación utilizando las retrotranscriptasas de la presente invención y a un kit que comprende los elementos necesarios para llevar a cabo dichos métodos.
ES201031864A 2010-12-16 2010-12-16 Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0 Withdrawn - After Issue ES2386264B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201031864A ES2386264B1 (es) 2010-12-16 2010-12-16 Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0
PCT/ES2011/070801 WO2012080541A1 (es) 2010-12-16 2011-11-21 Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo o

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201031864A ES2386264B1 (es) 2010-12-16 2010-12-16 Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0

Publications (2)

Publication Number Publication Date
ES2386264A1 true ES2386264A1 (es) 2012-08-14
ES2386264B1 ES2386264B1 (es) 2013-07-10

Family

ID=46244147

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201031864A Withdrawn - After Issue ES2386264B1 (es) 2010-12-16 2010-12-16 Nuevas retrotranscriptasas del virus de la inmunodeficiencia humana tipo 1 grupo 0

Country Status (2)

Country Link
ES (1) ES2386264B1 (es)
WO (1) WO2012080541A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2525135B1 (es) 2013-05-17 2015-09-29 Consejo Superior De Investigaciones Científicas (Csic) Retrotranscriptasas del vih tipo 1 grupo o, activas a temperaturas elevadas
US9920305B2 (en) 2013-10-16 2018-03-20 New England Biolabs, Inc. Reverse transcriptase with enhanced properties

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010130864A1 (es) * 2009-05-13 2010-11-18 Consejo Superior De Investigaciones Científicas (Csic) Retrotranscriptasa del vih-1 de grupo o modificada

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010130864A1 (es) * 2009-05-13 2010-11-18 Consejo Superior De Investigaciones Científicas (Csic) Retrotranscriptasa del vih-1 de grupo o modificada

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ÁLVAREZ, M., MATAMOROS, T., MENÉNDEZ-ARIAS, L. Increased thermostability and fidelity of DNA synthesis of wild-type and mutant HIV-1 group O reverse transcriptases. Journal of Molecular Biology. Octubre 2009, Vol. 392, Nº 4, páginas 872 - 884. ISSN 1089-8638. *
DAS, K., BANDWAR, R. P., WHINE, K. L. et al. Structural basis for the role of the K65Rmutation in HIV-1 reverse transcriptase polymerization, excision antagonism, and tenofovir resistance. Journal of Biological Chemistry. Diciembre 2009, Vol. 284, Nº 50, páginas 35092-35100. ISSN 1083-351X. *
GARFORTH, S. J., DOMAOAL, R. A., LWATULA, C. et al. K65R and K65A Substitutions in HIV-1 reverse transcriptase enhance polymerase fidelity by decreasing both dNTP misinsertion and mispaired primer extension efficiencies. Journal of Molecular Biology. Agosto 2010, Vol 401, Nº 1, páginas 33-44. ISSN 0022-2836. *
MATAMOROS, T., KIM, B., MENÉNDEZ-ARIAS, L. Mechanistic insights into the role of Val75 of HIV-1 reverse transcriptase in misinsertion and mispair extension fidelity of DNA synthesis. Journal of Molecular Biology. Noviembre 2007, Vol. 375, Nº 5, páginas 1234-1248.ISSN 0022-2836. *
QUIÑONES-MATEU, M. E., SORIANO, V., DOMINGO, E., MENÉNDEZ-ARIAS, L. Characterization of the reverse transcriptase of a human immunodeficiency virus type 1 group O isolate. Virology. Septiembre 1997, Vol. 236, Nº 2, páginas 364-373. ISSN 0042-6822. *
SHAH, F. S., CURR, K. A., HAMBURGH, M. E. et al. Differential influence of nucleosideanalog-resistance mutations K65R and L74V on the overall mutation rate and error specificity of human immunodeficiency virus type 1 reverse transcriptase. The Journal of Biological Chemistry. Septiembre 2000, Vol. 275, Nº 35, páginas 27037-27044.ISSN 1359-6535. *

Also Published As

Publication number Publication date
ES2386264B1 (es) 2013-07-10
WO2012080541A8 (es) 2013-03-21
WO2012080541A1 (es) 2012-06-21

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