ES2232276B1 - SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL. - Google Patents
SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL.Info
- Publication number
- ES2232276B1 ES2232276B1 ES200300860A ES200300860A ES2232276B1 ES 2232276 B1 ES2232276 B1 ES 2232276B1 ES 200300860 A ES200300860 A ES 200300860A ES 200300860 A ES200300860 A ES 200300860A ES 2232276 B1 ES2232276 B1 ES 2232276B1
- Authority
- ES
- Spain
- Prior art keywords
- tocopherol
- beta
- seeds
- tocopherols
- sunflower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Aceite de semilla de girasol con alto contenido en beta- tocoferol. La presente invención se refiere a un aceite de semillas de girasol modificadas genéticamente a través de dos ciclos de inducción artificial de mutaciones seguidos en cada caso por procesos de identificación de individuos mutantes que poseen el carácter deseado. Dicho aceite se caracterizan por poseer entre el 51% y el 85% de los tocoferoles en forma de beta tocoferol. Esta elevada producción de beta tocoferol está determinada por el genotipo de las semillas, que ha sido modificado con este objetivo, y se produce siempre con independencia de las condiciones de cultivo, tratándose por tanto de un carácter heredable. No existen en la actualidad semillas de girasol que produzcan niveles de beta tocoferol tan elevados.Sunflower seed oil with high beta-tocopherol content. The present invention relates to an oil of sunflower seeds genetically modified through two cycles of artificial induction of mutations followed in each case by identification processes of mutant individuals possessing the desired character. Said oil is characterized by having between 51% and 85% of tocopherols in the form of beta tocopherol. This high production of beta tocopherol is determined by the genotype of the seeds, which has been modified for this purpose, and is always produced independently of the growing conditions, thus being an inheritable character. There are currently no sunflower seeds that produce such high levels of beta tocopherol.
Description
Aceite de semilla de girasol con alto contenido en beta-tocoferol.Sunflower seed oil with high content in beta-tocopherol.
La invención se enmarca en el sector de la agricultura, tratándose de semillas con elevado contenido en beta-tocoferol. El aceite extraído de las semillas posee una gran estabilidad oxidativa y es óptimo para usos alimentarios e industriales (biocombustibles y lubricantes). Este aceite y sus subproductos pueden emplearse para extracción de beta-tocoferol, con numerosas aplicaciones en las industrias alimentaria, cosmética y farmacéutica.The invention is part of the sector of agriculture, in the case of seeds with high content in beta-tocopherol The oil extracted from the seeds It has great oxidative stability and is optimal for uses food and industrial (biofuels and lubricants). This oil and its by-products can be used to extract beta-tocopherol, with numerous applications in Food, cosmetic and pharmaceutical industries.
Los tocoferoles son los principales compuestos naturales con propiedades antioxidantes presentes en los aceites de semillas. Al ser liposolubles, pasan al aceite durante el proceso de extracción y ejercen una importante actividad antioxidante, tanto en el aceite envasado como en los alimentos que contienen aceite vegetal (F.B. Padley y col., 1994; Occurrence and characteristics of oils and fats. En The Lipid Handbook, ed. F.D. Gunstone, J.L. Harwood y F.B. Padley, London: Chapman & Hall, pp 47-223). De los cuatro tipos de tocoferoles que existen (alfa-, beta-, gamma-, y delta-tocoferol), la máxima actividad antioxidante in vitro, es decir, fuera del organismo humano, corresponde a beta-, gamma-, y delta-tocoferol, mientras que alfa-tocoferol presenta una actividad in vitro notablemente inferior a los otros tres tocoferoles (G. Pongracz y col., Tocopherole, Antioxidanten der Natur. Fat Science and Technology 97: 90-104, 1995).Tocopherols are the main natural compounds with antioxidant properties present in seed oils. Being fat-soluble, they pass into the oil during the extraction process and exert an important antioxidant activity, both in packaged oil and in foods that contain vegetable oil (FB Padley et al., 1994; Occurrence and characteristics of oils and fats. The Lipid Handbook , ed. FD Gunstone, JL Harwood and FB Padley, London: Chapman & Hall, pp 47-223). Of the four types of tocopherols that exist (alpha-, beta-, gamma-, and delta-tocopherol), the maximum antioxidant activity in vitro , that is, outside the human organism, corresponds to beta-, gamma-, and delta- tocopherol, while alpha-tocopherol has significantly lower in vitro activity than the other three tocopherols (G. Pongracz et al., Tocopherole, Antioxidanten der Natur. Fat Science and Technology 97: 90-104, 1995).
Las semillas de girasol estándar presentan una fracción de tocoferoles dominada por alfa-tocoferol, que representa aproximadamente el 95% del total de tocoferoles, estando el resto constituido por beta-tocoferol y gamma-tocoferol, que se encuentran presentes en proporciones inferiores al 5% del total de tocoferoles (F.B. Padley y col., 1994, obra citada). Debido a la predominancia de alfa-tocoferol en las semillas de girasol, su aceite presenta una menor protección frente a la oxidación que otros aceites vegetales extraídos a partir de semillas que contienen mayores proporciones de beta-, gamma-, y/o delta-tocoferol, que ejercen una mayor actividad antioxidante in vitro. La Tabla 1 presenta la composición en tocoferoles de los principales aceites de semillas.Standard sunflower seeds have a fraction of tocopherols dominated by alpha-tocopherol, which represents approximately 95% of the total tocopherols, the rest being made up of beta-tocopherol and gamma-tocopherol, which are present in proportions below 5% of total tocopherols (FB Padley et al., 1994, cited work). Due to the predominance of alpha-tocopherol in sunflower seeds, its oil has less protection against oxidation than other vegetable oils extracted from seeds that contain higher proportions of beta-, gamma-, and / or delta-tocopherol , which exert a greater antioxidant activity in vitro . Table 1 presents the tocopherol composition of the main seed oils.
\vskip1.000000\baselineskip\ vskip1.000000 \ baselineskip
La predominancia de alfa-tocoferol en las semillas de girasol es prácticamente universal, habiéndose descrito únicamente cuatro líneas de girasol que presentan niveles modificados de tocoferoles, y que se pueden agrupar en dos clases:The predominance of alpha-tocopherol in sunflower seeds is virtually universal, having described only four sunflower lines that have modified levels of tocopherols, and that can be grouped into two classes:
Se trata de dos líneas que poseen más del 85% de los tocoferoles en forma de gamma-tocoferol, siendo el resto alfa-tocoferol. Una de ellas, denominada LG-17, fue desarrollada en Rusia (Y. Demurin, Genetic variability of tocopherol composition in sunflower seeds, Helia 16:59-62, 1993), mientras que la segunda fue desarrollada en España y se denominó T2100 (L. Velasco y col., Registration of T589 and T2100 sunflower germplasms with modified tocopherol profiles, Crop Science, en prensa).These are two lines that have more than 85% of tocopherols in the form of gamma-tocopherol, being the rest alpha-tocopherol. One of them, called LG-17, was developed in Russia (Y. Demurin, Genetic variability of tocopherol composition in sunflower seeds, Helia 16: 59-62, 1993), while the second was developed in Spain and named T2100 (L. Velasco et al., Registration of T589 and T2100 sunflower germplasms with modified tocopherol profiles, Crop Science, in press).
Se trata de dos líneas que poseen entre el 30% y el 50% de los tocoferoles de la semilla en forma de beta-tocoferol, siendo el resto alfa-T. Una de ellas, denominada LG-15, fue desarrollada en Rusia (Y. Demurin, 1993, obra citada), mientras que la segunda, denominada T589, fue desarrollada en España (L. Velasco y col., en prensa, obra citada).These are two lines that have between 30% and 50% of the tocopherols of the seed in the form of beta-tocopherol, the rest being Alpha-T One of them, called LG-15, was developed in Russia (Y. Demurin, 1993, cited work), while the second, called T589, was developed in Spain (L. Velasco et al., in press, work cited).
Mediante cruzamiento entre las líneas LG-15 y LG-17, investigadores rusos y yugoslavos obtuvieron recombinantes con niveles ligeramente elevados de delta- tocoferol, siendo el máximo nivel obtenido de este tocoferol del 25% de todos los tocoferoles presentes en la semilla (Y. Demurin y col. Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality. Plant Breeding 115:33-36, 1996). En resumen, los niveles máximos de tocoferoles individuales que existen hoy día en semillas de girasol son:By crossing between the lines LG-15 and LG-17, Russian researchers and Yugoslavs obtained recombinants with slightly levels high levels of deltatocopherol, being the highest level obtained from this 25% tocopherol of all tocopherols present in the seed (Y. Demurin et al. Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality Plant Breeding 115: 33-36, 1996). In summary, the maximum levels of individual tocopherols that They exist today in sunflower seeds are:
\sqbullet 95% alfa-tocoferol (composición natural)95% alpha-tocopherol (natural composition)
\sqbullet 50% beta-tocoferol\ sqbullet 50% beta-tocopherol
\sqbullet 95% gamma-tocoferol\ sqbullet 95% gamma-tocopherol
\sqbullet 25% delta-tocoferol\ sqbullet 25% delta-tocopherol
Uno de los objetos de la presente invención es el aceite de semillas de girasol con alto contenido en beta-tocoferol, que presentan entre el 51% y el 85% del total de tocoferoles en forma de beta-tocoferol, y los siguientes contenidos de otros tocoferoles: entre el 20% y el 49% del total de tocoferoles en forma de alfa-tocoferol; entre el 0% y el 2% del total de tocoferoles en forma de gamma- tocoferol; entre el 0% y el 25% del total de tocoferoles en forma de delta- tocoferol. Algunos de estos aceites presentan un contenido en beta-tocoferol siempre superior al 60%, 70% y 80% del total de tocoferoles en las semillas. El carácter de alto contenido en beta-tocoferol de las semillas de girasol, a partir de las cuales se obtiene el aceite, es heredable (al ser autofecundadas) y se expresan de forma estable, independientemente de las condiciones ambientales.One of the objects of the present invention is the sunflower seed oil with high content in beta-tocopherol, which have between 51% and 85% of total tocopherols in the form of beta-tocopherol, and the following contents of other tocopherols: between 20% and 49% of total tocopherols in alpha-tocopherol form; between 0% and 2% of total tocopherols in the form of gamma-tocopherol; between 0% and 25% of total tocopherols in the form of deltatocopherol. Some of these oils have a content in beta-tocopherol always exceeding 60%, 70% and 80% of total tocopherols in the seeds. High character beta-tocopherol content of the seeds of Sunflower, from which the oil is obtained, is inheritable (being self-fertilized) and expressing themselves stably, regardless of environmental conditions.
La presente invención se refiere a un germoplasma de girasol (Helianthus annuus L.) caracterizado por poseer un elevado contenido de beta-tocoferol en las semillas. Este tocoferol representa entre el 51% y el 85% del total de tocoferoles en las semillas. Estos elevados niveles de beta-tocoferol no son producidos en la naturaleza por plantas de girasol y han sido obtenidos mediante un proceso complejo consistente en dos ciclos de inducción artificial de mutaciones seguidos de identificación de plantas mutantes y fijación del carácter mutado. El elevado contenido en beta-tocoferol objeto de la presente invención es heredable y se produce siempre con independencia de las condiciones de cultivo.The present invention relates to a sunflower germplasm ( Helianthus annuus L.) characterized by having a high content of beta-tocopherol in the seeds. This tocopherol represents between 51% and 85% of the total tocopherols in the seeds. These high levels of beta-tocopherol are not produced in nature by sunflower plants and have been obtained through a complex process consisting of two cycles of artificial induction of mutations followed by identification of mutant plants and fixation of the mutated character. The high beta-tocopherol content object of the present invention is inheritable and is always produced independently of the culture conditions.
Para la obtención de las plantas modificadas genéticamente cuyas semillas poseen una elevada concentración de beta-tocoferol, se ha llevado a cabo un largo proceso de mejora genética dirigido a alterar genéticamente la ruta biosintética de tocoferoles. Este proceso ha consistido en cuatro etapas: (1) Inducción de mutaciones artificiales en semillas de una variedad estándar de girasol; (2) Identificación de individuos con alteraciones en la ruta biosintética de tocoferoles producto de las mutaciones inducidas y fijación de los caracteres mutados; (3) Nuevo proceso de inducción de mutaciones artificiales sobre individuos que ya presentaban un primer nivel de alteración en la ruta de biosíntesis de tocoferoles; (4) Identificación de individuos con alteraciones en la ruta biosintética de tocoferoles diferentes a las alteraciones que presentaban los individuos de partida, seguida de fijación del nuevo carácter mutante.To obtain the modified plants genetically whose seeds have a high concentration of beta-tocopherol, has been carried out a long genetic improvement process aimed at genetically altering the route Tocopherols biosynthetic. This process has consisted of four Stages: (1) Induction of artificial mutations in seeds of a standard sunflower variety; (2) Identification of individuals with alterations in the biosynthetic path of tocopherols product of the induced mutations and fixation of mutated characters; (3) New induction process of artificial mutations on individuals who already had a first level of alteration in the tocopherol biosynthesis path; (4) Identification of individuals with alterations in the biosynthetic path of tocopherols different from the alterations that the individuals of heading, followed by fixation of the new mutant character.
El primer proceso de mutagénesis o inducción artificial de mutaciones consistió en el tratamiento de semillas de una variedad estándar de girasol con un producto con propiedades mutagénicas, esto es, capaz de inducir mutaciones en el ADN de la planta. Debido a la baja frecuencia de mutaciones esperables en los genes responsables de la ruta biosintética de los tocoferoles tras el tratamiento mutagénico, éste fue seguido de un proceso de análisis no destructivo de la composición en tocoferoles en varios miles de semillas individuales.The first process of mutagenesis or induction artificial mutations consisted of the treatment of seeds of a standard variety of sunflower with a product with properties mutagenic, that is, capable of inducing mutations in the DNA of the plant. Due to the low frequency of expected mutations in the genes responsible for the biosynthetic pathway of tocopherols after the mutagenic treatment, this was followed by a process of non-destructive analysis of the composition in tocopherols in several thousands of individual seeds.
Para que las mutaciones detectadas resulten de utilidad comercial, deben ser heredables y deben expresarse con independencia de las condiciones ambientales en las que se cultivan las plantas. Por este motivo, se realizó un proceso de selección conducente a fijar los caracteres mutantes y a verificar su estabilidad bajo diferentes condiciones ambientales. Tras este proceso, varias de las mutaciones inicialmente detectadas fueron descartadas, mientras que un mutante con elevado contenido en gamma-tocoferol (95% del total de los tocoferoles presentes en las semillas) fue fijado. Este mutante, denominado IAST-1, demostró responder a una base genética diferente a la que poseen otras líneas con elevado contenido en gamma-tocoferol. Así, mientras que los cruzamientos de las líneas LG-17 y T2100 con líneas de composición estándar en tocoferoles producen progenies F_{2} que no segregan para niveles intermedios de gamma-tocoferol (Demurin y col., obra citada; L. Velasco y J.M. Fernández-Martínez, Identification and genetic characterization of new sources of beta- and gamma-tocopherol in sunflower germplasm, Helia, en prensa), las progenies F_{2} procedentes de cruces entre el mutante IAST-1 y líneas de composición estándar en tocoferoles segregaron ampliamente para niveles intermedios de gamma-tocoferol.So that the mutations detected result from commercial utility, must be inheritable and must be expressed with independence of the environmental conditions in which they are grown the plants. For this reason, a selection process was carried out conducive to setting mutant characters and verifying their stability under different environmental conditions. After this process, several of the initially detected mutations were discarded, while a mutant with high content in gamma-tocopherol (95% of total tocopherols present in the seeds) was fixed. This mutant, called IAST-1, demonstrated to respond to a genetic basis different from that of other lines with high content in gamma-tocopherol. So while the crosses of the LG-17 and T2100 lines with lines of standard composition in tocopherols produce F 2 progenies that do not segregate for intermediate levels of gamma-tocopherol (Demurin et al., cited work; L. Velasco and J.M. Fernández-Martínez, Identification and genetic characterization of new sources of beta- and gamma-tocopherol in sunflower germplasm, Helia, in press), the F2 progenies from crossings between the IAST-1 mutant and standard composition lines in tocopherols widely segregated for intermediate levels of gamma-tocopherol.
Tras el aislamiento genético del mutante IAST-1, se realizó un segundo proceso de mutagénesis sobre semillas de este mutante, con el objetivo de generar variación adicional para niveles elevados de otros tocoferoles. Este segundo ciclo de mutagénesis estuvo asimismo seguido de un proceso analítico a gran escala para identificación de mutantes, así como de un proceso de fijación de mutantes y confirmación de su expresión con independencia de las condiciones de cultivo. En este segundo ciclo de mutagénesis se identificó y fijó el mutante objeto de la presente invención, que se caracteriza porque sus semillas forman beta-tocoferol como tocoferol mayoritario, estando presente en una concentración comprendida entre el 51% y el 85% del total de tocoferoles en las semillas. Esta elevada proporción de beta-tocoferol en las semillas es un carácter heredable y se expresa de forma estable independientemente de las condiciones de cultivo de las plantas. El aceite extraído de estas semillas presenta el mismo perfil de tocoferoles (51-85% de beta-tocoferol del total de tocoferoles)After genetic isolation of the mutant IAST-1, a second process of seed mutagenesis of this mutant, with the aim of generate additional variation for high levels of others tocopherols. This second cycle of mutagenesis was also followed by a large-scale analytical process for identification of mutants, as well as a process of fixing mutants and confirmation of your expression regardless of the conditions of culture. In this second cycle of mutagenesis, it was identified and fixed the mutant object of the present invention, which is characterized because its seeds form beta-tocopherol as major tocopherol, being present in a concentration between 51% and 85% of the total tocopherols in the seeds. This high proportion of beta-tocopherol in the seeds it is an inheritable character and is expressed in a way stable regardless of the culture conditions of the plants. The oil extracted from these seeds has the same tocopherol profile (51-85% of total tocopherol beta-tocopherol)
Semillas de girasol de la variedad población Peredovik, con una composición en tocoferoles en las semillas consistente en 96% alfa-tocoferol, 3% beta- tocoferol, y 1% gamma-tocoferol, se embebieron durante 4 horas en agua destilada, a una temperatura de 20ºC. Pasado este tiempo, las semillas se transfirieron a una solución del agente mutagénico metilsulfonato de etilo (EMS) con una concentración 70 mM en tampón fosfato 0.1M a pH 7.0 durante 2 horas, manteniendo una agitación constante de 60 rpm. Tras el tratamiento mutagénico, las semillas (generación M_{1}) se lavaron durante 16 horas con agua corriente y posteriormente se sembraron en el campo.Sunflower seeds of the population variety Peredovik, with a tocopherol composition in the seeds consisting of 96% alpha-tocopherol, 3% beta- tocopherol, and 1% gamma-tocopherol, were embedded for 4 hours in distilled water, at a temperature of 20 ° C. After this time, the seeds were transferred to a solution of the ethyl methylsulfonate mutagenic agent (EMS) with a 70 mM concentration in 0.1M phosphate buffer at pH 7.0 for 2 hours, maintaining a constant stirring of 60 rpm. After treatment mutagenic, the seeds (M1 generation) were washed for 16 hours with running water and subsequently planted in the countryside.
Las plantas M_{1} se cosecharon individualmente
y sus semillas (generación M_{2}) se analizaron
individualizadamente para composición en tocoferoles mediante
cromatografia líquida de alta eficacia (HPLC), siguiendo el
protocolo desarrollado por F. Goffman y col. (Quantitative
determination of tocopherols in single seeds of rapeseed
[Brassica napus L.]. Fett/Lipid
101:142-145, 1999). De un total de 1080 plantas
M_{1} analizadas, una de ellas presentó segregación para niveles
elevados de gamma-tocoferol, con un máximo contenido
de 95% de los tocoferoles totales en forma de
gamma-tocoferol. Semillas con estos niveles de
gamma-tocoferol produjeron plantas que expresaron el
carácter uniformemente. Al cruzar plantas procedentes de semillas
con 95% de gamma- tocoferol con plantas de variedades estándar de
girasol, se observó un amplia segregación para contenido en
gamma-tocoferol en semillas F_{2}, que incluyó
niveles de gamma-tocoferol intermedios entre ambos
parentales. Esta segregación tan amplia fue completamente
inesperada, debido a que materiales con niveles similares de
gamma-tocoferol desarrollados previamente (Demurin y
col., obra citada; L. Velasco y J.M.
Fernández-Martínez, obra citada) no habían producido
segregación para niveles intermedios de
gamma-tocoferol tras ser cruzadas con variedades
estándar de girasol. El mutante así obtenido se denominó
IAST-1.The M 1 plants were harvested individually and their seeds (M 2 generation) were analyzed individually for tocopherol composition by high efficiency liquid chromatography (HPLC), following the protocol developed by F. Goffman et al. (Quantitative determination of tocopherols in single seeds of rapeseed [ Brassica napus L.]. Fett / Lipid 101: 142-145, 1999). Of a total of 1080 M1 plants analyzed, one of them presented segregation for high levels of gamma-tocopherol, with a maximum content of 95% of total tocopherols in the form of gamma-tocopherol. Seeds with these levels of gamma-tocopherol produced plants that expressed the character evenly. When crossing plants from seeds with 95% gamma-tocopherol with plants of standard sunflower varieties, a wide segregation was observed for gamma-tocopherol content in F2 seeds, which included intermediate gamma-tocopherol levels between both parents. . This wide segregation was completely unexpected, because materials with similar levels of gamma-tocopherol previously developed (Demurin et al., Cited work; L. Velasco and JM Fernández-Martínez, cited work) had not produced segregation for intermediate levels of Gamma-tocopherol after being crossed with standard sunflower varieties. The mutant thus obtained was named
IAST-1
Semillas de girasol del mutante IAST-1, con una composición en tocoferoles en las semillas consistente en 5% alfa-tocoferol y 95% gamma-tocoferol, se embebieron durante 4 horas en agua destilada, a una temperatura de 20ºC. Pasado este tiempo, las semillas se transfirieron a una solución del agente mutagénico azida sódica con una concentración 4 mM en tampón 0.1 M citrato sódico a pH 3.0 durante 2 horas, manteniendo una agitación constante de 60 rpm. Tras el tratamiento mutagénico, las semillas (generación M_{1}) se lavaron durante 16 horas con agua corriente y posteriormente se sembraron en el campo.Mutant sunflower seeds IAST-1, with a composition in tocopherols in the seeds consisting of 5% alpha-tocopherol and 95% gamma-tocopherol, were embedded for 4 hours in distilled water, at a temperature of 20 ° C. After this time, the seeds were transferred to a solution of the mutagenic agent sodium azide with a 4 mM concentration in 0.1 M citrate buffer sodium at pH 3.0 for 2 hours, while stirring 60 rpm constant. After the mutagenic treatment, the seeds (generation M1) were washed for 16 hours with running water and later they were sown in the field.
Las plantas M_{1} se cosecharon individualmente y sus semillas (generación M_{2}) se analizaron individualizadamente para composición en tocoferoles mediante cromatografia líquida de alta eficacia (HPLC), siguiendo el protocolo desarrollado por F. Goffman y col. (obra citada). De un total de 1240 plantas M_{1} analizadas, una de ellas presentó segregación para niveles elevados de beta-tocoferol, con un máximo contenido de 65% de los tocoferoles totales en forma de beta-tocoferol. Semillas con estos niveles de beta-tocoferol produjeron plantas que expresaron el carácter uniformemente, con concentraciones de beta-tocoferol comprendidas entre 51% y 85% del total de tocoferoles presentes en las semillas. Estos niveles se mantuvieron en sucesivas generaciones. La nueva línea mutante de girasol cuyas semillas producen niveles de beta-tocoferol comprendidos entre el 51% y el 85% del total de tocoferoles se denominó IAST-2.M1 plants were harvested individually and its seeds (generation M_ {2}) were analyzed individually for composition in tocopherols by high efficiency liquid chromatography (HPLC), following the protocol developed by F. Goffman et al. (cited work). Of a total of 1240 M1 plants analyzed, one of them presented segregation for high levels of beta-tocopherol, with a maximum content of 65% of Total tocopherols in the form of beta-tocopherol. Seeds with these levels of beta-tocopherol they produced plants that expressed the character evenly, with beta-tocopherol concentrations included between 51% and 85% of the total tocopherols present in the seeds. These levels were maintained in successive generations. The new one mutant sunflower line whose seeds produce levels of beta-tocopherol between 51% and 85% of the total tocopherols was named IAST-2.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200300860A ES2232276B1 (en) | 2003-04-10 | 2003-04-10 | SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200300860A ES2232276B1 (en) | 2003-04-10 | 2003-04-10 | SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2232276A1 ES2232276A1 (en) | 2005-05-16 |
| ES2232276B1 true ES2232276B1 (en) | 2006-10-01 |
Family
ID=34586107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200300860A Expired - Fee Related ES2232276B1 (en) | 2003-04-10 | 2003-04-10 | SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2232276B1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2082717B1 (en) * | 1994-01-31 | 1996-12-16 | Consejo Superior Investigacion | VERY HIGH SUNFLOWER SEED OIL CONTAINED IN STEARIC ACID. |
| FR2778527A1 (en) * | 1998-05-18 | 1999-11-19 | Rhone Poulenc Agrochimie | Producing tomato, cereal or oilseed plants, especially maize, oilseed rape, soya or sunflower plants, from which tocopherols can be extracted and purified |
| BR9913329A (en) * | 1998-08-25 | 2002-01-15 | Univ Nevada | Manipulation of tocopherol levels in transgenic plants |
-
2003
- 2003-04-10 ES ES200300860A patent/ES2232276B1/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| DEMURIN, Y. et al.: "Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality", Plant Breeding, 1996, Vol. 115, nº 1, páginas 33-36, ISSN: 0179-9541, todo el documento. * |
| VELASCO, L. et al.: "Genetic and environmental variation for tocopherol content and composition in sunflower commercial hybrids", J. Agric. Sci., 2002, Vol. 139, nº 4, páginas 425-429, ISSN: 0021-8596, todo el documento. * |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2232276A1 (en) | 2005-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| D’Antuono et al. | Glucosinolates in Diplotaxis and Eruca leaves: Diversity, taxonomic relations and applied aspects | |
| Vera et al. | Comparison of the lipid profile and tocopherol content of four Peruvian quinoa (Chenopodium quinoa Willd.) cultivars (‘Amarilla de Maranganí’,‘Blanca de Juli’, INIA 415 ‘Roja Pasankalla’, INIA 420 ‘Negra Collana’) during germination | |
| CA2692687C (en) | Brassica plant comprising mutant fatty acyl-acp thioesterase alleles | |
| JP2021120453A (en) | Sunflower oil with high heat stability | |
| Morcillo et al. | Natural diversity in the carotene, tocochromanol and fatty acid composition of crude palm oil | |
| Grüneberg et al. | Characterization of yam bean (Pachyrhizus spp.) seeds as potential sources of high palmitic acid oil | |
| Fernández-Cuesta et al. | Tocopherols and phytosterols in sunflower seeds for the human food market | |
| Vassilevska-Ivanova et al. | Utilization of related wild species (Echinacea purpurea) for genetic enhancement of cultivated sunflower (Helianthus annuus L.) | |
| BRPI0821333A2 (en) | SUNFLOWER SEED OIL WITH HIGH HEAT STABILITY, OIL MIXTURES, FLOUR, SUNFLOWER SEEDS, SUNFLOWER PLANTS, USE OF SUNFLOWER SEEDS, AND USE OF SUNFLOWER SEED OIL. | |
| ES2222908T5 (en) | SEED OIL WITH A MODIFIED FATTY ACIDS COMPOSITION. | |
| ES2233174B1 (en) | SUNFLOWER SEED OIL WITH HIGH CONTENT IN DELTA-TOCOFEROL. | |
| ES2232276B1 (en) | SUNFLOWER SEED OIL WITH HIGH CONTENT IN BETA-TOCOFEROL. | |
| KR20190089012A (en) | Arctic Bramble (Lubus Arcticus) cell culture, method for producing Arctic rubble cell culture, composition comprising Arctic rubble cell culture, and use of Arctic rubble cell culture | |
| Nabloussi et al. | Moroccan sesame: An overview of seed and oil quality | |
| Singh et al. | Antioxidant and phytochemical levels and their interrelation in stem and leaf extract of water spinach (Ipomea aquatica) | |
| Fahmy et al. | Genetic Improvement of Calendula officinal through Mutation Induction using Gamma Irradiation and Chemical Mutagens | |
| KR101557417B1 (en) | Brassica rapa having increased vitamin C content and breeding method thereof | |
| Cvejić et al. | Development and utilization of sunflower genotypes with altered oil quality | |
| Škorić et al. | Genetic variability of oil quality components in sunflower as a function of developing hybrids with novel oil | |
| EL-MOATY et al. | ASSESMENT OF PRIMARY METABOLITES OF HELICHRYSUM CONGLOBATUM (VIV.) STEUD. DURING DIFFERENT GROWTH SEASONS. | |
| Saeed et al. | A jaunt through sunflower: from its origin to the different breeding approaches for its improvement | |
| Trépanier | Impact of seeding date on the accumulation of tocopherols in soybeans | |
| Skoric et al. | Genetic variability of oil quality components in sunflower as a function of developing hybrids with novel oiL | |
| Mariod et al. | Organic foods from Annona squamosa (Gishta) and Catunaregam nilotica (Kirkir) | |
| Iqbal Ahmed et al. | Heterosis studies for seed yield and its components in experimental hybrids based on CMS lines in safflower (Carthamus tinctorius L.). |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20050516 Kind code of ref document: A1 |
|
| FG2A | Definitive protection |
Ref document number: 2232276B1 Country of ref document: ES |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20180808 |