ES2192426B1 - Anticuerpo monoclonal humano sm50/20 que reconoce leucocitos humanos, y su uso en terapia. - Google Patents
Anticuerpo monoclonal humano sm50/20 que reconoce leucocitos humanos, y su uso en terapia.Info
- Publication number
- ES2192426B1 ES2192426B1 ES200001194A ES200001194A ES2192426B1 ES 2192426 B1 ES2192426 B1 ES 2192426B1 ES 200001194 A ES200001194 A ES 200001194A ES 200001194 A ES200001194 A ES 200001194A ES 2192426 B1 ES2192426 B1 ES 2192426B1
- Authority
- ES
- Spain
- Prior art keywords
- human
- lymphocytes
- present
- monoclonal antibody
- leukocytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000265 leukocyte Anatomy 0.000 title abstract 2
- 101100257123 Strongylocentrotus purpuratus SM50 gene Proteins 0.000 title 1
- 238000002560 therapeutic procedure Methods 0.000 title 1
- 241001529936 Murinae Species 0.000 abstract 2
- 241000699670 Mus sp. Species 0.000 abstract 2
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 2
- 210000004408 hybridoma Anatomy 0.000 abstract 2
- 230000007170 pathology Effects 0.000 abstract 2
- 238000002965 ELISA Methods 0.000 abstract 1
- 108700005091 Immunoglobulin Genes Proteins 0.000 abstract 1
- 206010025323 Lymphomas Diseases 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 206010035226 Plasma cell myeloma Diseases 0.000 abstract 1
- 210000001744 T-lymphocyte Anatomy 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000005859 cell recognition Effects 0.000 abstract 1
- 230000001684 chronic effect Effects 0.000 abstract 1
- 208000032852 chronic lymphocytic leukemia Diseases 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 210000003714 granulocyte Anatomy 0.000 abstract 1
- 210000005096 hematological system Anatomy 0.000 abstract 1
- 238000010166 immunofluorescence Methods 0.000 abstract 1
- 208000032839 leukemia Diseases 0.000 abstract 1
- 210000001616 monocyte Anatomy 0.000 abstract 1
- 201000000050 myeloid neoplasm Diseases 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención corresponde a Hibridoma productor de un anticuerpo monoclonal humano SM 50/20 (isotipo IgM / kappa) con cadenas pesadas y ligeras humanas. El anticuerpo reconoce un antígeno específico (peso molecular 150-180 Kd.) presente en linfocitos T y linfocitos B, monocitos, granulocitos, líneas tumorales humanas tipo B y T, y leucemias humanas linfoides crónicas, y algunas mieloides. El hibridoma se obtuvo de linfocitos B de ratones inmunizados con leucocitos humanos fusionados con células de mieloma murino SP2/0, posterior clonación y seleccionado en base a su secreción de anticuerpos por ELISA y a su patrón de reconocimiento celular por inmunofluorescencia indirecta. Los ratones inmunizados son transgénicos, portadores de genes de inmunoglobulinas humanas (m y k )y carecen de los genes murinos de cadenas pesadas y ligera kappa. La presente invención podría utilizarse para detección y/o tratamiento de patología linfoide humana (leucemia linfoide crónica, linfomas y otras patologías del sistema hematológico).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200001194A ES2192426B1 (es) | 2000-05-11 | 2000-05-11 | Anticuerpo monoclonal humano sm50/20 que reconoce leucocitos humanos, y su uso en terapia. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200001194A ES2192426B1 (es) | 2000-05-11 | 2000-05-11 | Anticuerpo monoclonal humano sm50/20 que reconoce leucocitos humanos, y su uso en terapia. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2192426A1 ES2192426A1 (es) | 2003-10-01 |
| ES2192426B1 true ES2192426B1 (es) | 2005-02-16 |
Family
ID=29762849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200001194A Expired - Fee Related ES2192426B1 (es) | 2000-05-11 | 2000-05-11 | Anticuerpo monoclonal humano sm50/20 que reconoce leucocitos humanos, y su uso en terapia. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2192426B1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201419094D0 (en) | 2014-10-27 | 2014-12-10 | Agency Science Tech & Res | Anti-TIM-3-antibodies |
| CN110294807B (zh) | 2014-10-27 | 2023-05-12 | 新加坡科技研究局 | 抗tim-3抗体 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6255458B1 (en) * | 1990-08-29 | 2001-07-03 | Genpharm International | High affinity human antibodies and human antibodies against digoxin |
| US5877397A (en) * | 1990-08-29 | 1999-03-02 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| CA2072249C (en) * | 1991-06-28 | 2003-06-17 | Saiko Hosokawa | Human monoclonal antibody specifically binding to surface antigen of cancer cell membrane |
| EP1978033A3 (en) * | 1995-04-27 | 2008-12-24 | Amgen Fremont Inc. | Human antibodies derived from immunized xenomice |
-
2000
- 2000-05-11 ES ES200001194A patent/ES2192426B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2192426A1 (es) | 2003-10-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20031001 Kind code of ref document: A1 |
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| FG2A | Definitive protection |
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| FD2A | Announcement of lapse in spain |
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