ES2190051T3 - Procedimiento de calibrado de un sistema de dosificacion de ligando-antiligando. - Google Patents

Procedimiento de calibrado de un sistema de dosificacion de ligando-antiligando.

Info

Publication number
ES2190051T3
ES2190051T3 ES98430022T ES98430022T ES2190051T3 ES 2190051 T3 ES2190051 T3 ES 2190051T3 ES 98430022 T ES98430022 T ES 98430022T ES 98430022 T ES98430022 T ES 98430022T ES 2190051 T3 ES2190051 T3 ES 2190051T3
Authority
ES
Spain
Prior art keywords
coupled
antiligand
specific
analyte
microspheres
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98430022T
Other languages
English (en)
Inventor
Vincent Fert
Michel Delaage
Christophe Camilla
Jean-Philippe Defoort
Sabine Prato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IMMUNOTECH SA
Immunotech SAS
Original Assignee
IMMUNOTECH SA
Immunotech SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IMMUNOTECH SA, Immunotech SAS filed Critical IMMUNOTECH SA
Application granted granted Critical
Publication of ES2190051T3 publication Critical patent/ES2190051T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Testing, Inspecting, Measuring Of Stereoscopic Televisions And Televisions (AREA)
  • Testing Or Calibration Of Command Recording Devices (AREA)
  • Steroid Compounds (AREA)
  • Measurement Of Radiation (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

PROCEDIMIENTO DE CALIBRACION DE UN SISTEMA DE DOSIFICACION DE UN ANALITO EN UNA MUESTRA BIOLOGICA, QUE EMPLEA UNA INTERACCION ESPECIFICA DE TIPO LIGANDO/ANTILIGANDO EN UN ESQUEMA DE REACCION DE TIPO "SANDWICH" O "COMPETICION", CARACTERIZADO PORQUE SE HACE REACCIONAR LA MUESTRA BIOLOGICA CON UNA MEZCLA DE MICROESFERAS QUE COMPRENDE: - UNA PRIMERA CATEGORIA DE MICROESFERAS CARACTERIZABLE POR SU DIMENSION, EL ESPECTRO DE FLUORESCENCIA (F1) O POR LA INTENSIDAD DE LA FLUORESCENCIA (F1), LLAMADAS MICROESFERAS DE CALIBRACION, INCLUYENDO ESTAS MICROESFERAS UN SITIO LIGANDO E1, Y CON - UN TRAZADOR FORMADO POR UN ANTILIGANDO ESPECIFICO DEL SITIO LIGANDO E 1 Y ACOPLADO A UN CO MPUESTO FLUOROFORO F2 DE UNA FLUORESCENCIA DIFERENTE A LA (F1) QUE PUEDE CARACTERIZAR LAS MICROESFERAS DE CALIBRACION Y CON UNA SEGUNDA CATEGORIA DE MICROESFERAS, LLAMADAS MICROESFERAS DE DOSIFICACIONES, DIFERENTE DE LA PRIMERA CATEGORIA DE MICROESFERAS EN EL PLANO O DE LA DIMENSION O DEL ESPECTRO DE FLUORESCENCIA (F1) O DE LA INTENSIDAD DE LA FLUORESCENCIA (F1), Y QUE CONSTITUYE EL SOPORTE DE LA REACCION ANALITICA, SEGUN 4 MODALIDADES DE PUESTA EN PRACTICA. KITS DE DOSIFICACION Y PROCEDIMIENTO DE DOSIFICACION DE UN ANALITO POR TECNICA LLAMADA "SANDWICH" O POR "COMPETICION" QUE UTILIZA UN PROCEDIMIENTO DE CALIBRACION ANTERIOR.
ES98430022T 1997-09-18 1998-09-17 Procedimiento de calibrado de un sistema de dosificacion de ligando-antiligando. Expired - Lifetime ES2190051T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9711848A FR2768519B1 (fr) 1997-09-18 1997-09-18 Procede d'etalonnage d'un systeme de dosage de type ligand - anti ligand

Publications (1)

Publication Number Publication Date
ES2190051T3 true ES2190051T3 (es) 2003-07-16

Family

ID=9511399

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98430022T Expired - Lifetime ES2190051T3 (es) 1997-09-18 1998-09-17 Procedimiento de calibrado de un sistema de dosificacion de ligando-antiligando.

Country Status (6)

Country Link
EP (1) EP0916951B1 (es)
AT (1) ATE230485T1 (es)
DE (1) DE69810429T2 (es)
DK (1) DK0916951T3 (es)
ES (1) ES2190051T3 (es)
FR (1) FR2768519B1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9005901B2 (en) 2013-03-15 2015-04-14 Abbott Laboratories Assay with internal calibration
US9157910B2 (en) 2013-03-15 2015-10-13 Abbott Laboratories Assay with increased dynamic range
CN111308064B (zh) * 2020-02-27 2024-04-09 四川新健康成生物股份有限公司 一种提升免疫层析标记物灵敏度的方法及在白介素6检测中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3322373C2 (de) * 1983-05-19 1986-12-04 Ioannis Dr. 3000 Hannover Tripatzis Testmittel und Verfahren zum Nachweis von Antigenen und/oder Antikörpern
US5093234A (en) * 1984-12-24 1992-03-03 Caribbean Microparticles Corporation Method of aligning, compensating, and calibrating a flow cytometer for analysis of samples, and microbead standards kit therefor
SE458968B (sv) * 1987-06-16 1989-05-22 Wallac Oy Biospecifikt analysfoerfarande foer flera analyter i vilket ingaar partikelraekning och maerkning med fluorescerande maerksubstanser

Also Published As

Publication number Publication date
DE69810429T2 (de) 2003-11-27
DE69810429D1 (de) 2003-02-06
ATE230485T1 (de) 2003-01-15
FR2768519A1 (fr) 1999-03-19
EP0916951A1 (fr) 1999-05-19
FR2768519B1 (fr) 1999-12-10
EP0916951B1 (fr) 2003-01-02
DK0916951T3 (da) 2003-05-05

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