ES2189878T3 - Deteccion y biodegradacion de explosivos. - Google Patents

Deteccion y biodegradacion de explosivos.

Info

Publication number
ES2189878T3
ES2189878T3 ES96922985T ES96922985T ES2189878T3 ES 2189878 T3 ES2189878 T3 ES 2189878T3 ES 96922985 T ES96922985 T ES 96922985T ES 96922985 T ES96922985 T ES 96922985T ES 2189878 T3 ES2189878 T3 ES 2189878T3
Authority
ES
Spain
Prior art keywords
enzyme
petn
procedure
produced
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96922985T
Other languages
English (en)
Inventor
Stephen Nicklin
Peter Roland Binks
Neil Charles Bruce
Christopher Edward French
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9514138A external-priority patent/GB2303136A/en
Priority claimed from GB9607220A external-priority patent/GB2311784A/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Application granted granted Critical
Publication of ES2189878T3 publication Critical patent/ES2189878T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C06—EXPLOSIVES; MATCHES
    • C06B—EXPLOSIVES OR THERMIC COMPOSITIONS; MANUFACTURE THEREOF; USE OF SINGLE SUBSTANCES AS EXPLOSIVES
    • C06B21/00—Apparatus or methods for working-up explosives, e.g. forming, cutting, drying
    • C06B21/0091—Elimination of undesirable or temporary components of an intermediate or finished product, e.g. making porous or low density products, purifying, stabilising, drying; Deactivating; Reclaiming
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/008—Preparation of nitrogen-containing organic compounds containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/06—Explosives, propellants or pyrotechnics, e.g. rocket fuel or napalm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Soil Sciences (AREA)
  • Mycology (AREA)
  • Hydrology & Water Resources (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Business, Economics & Management (AREA)
  • Emergency Management (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE UNA ENZIMA CAPAZ DE CATALIZAR LA ELIMINACION DE NITRITO DE TETRANITRATO DE PENTAERITRITOL (PETN). LA ENZIMA (CONOCIDA COMO ENZIMA PETN REDUCTASA) SE PRODUCE MEDIANTE EL CULTIVO DE UNA NUEVA CEPA DE LA BACTERIA ENTEROBACTER CLOACAE AISLADA DE LA NATURALEZA. LA CEPA DENOMINADA PB2 SE HA DEPOSITADO COMO NCIMB 40718. LA SECUENCIA DE AMINOACIDOS DE LA ENZIMA Y LA SECUENCIA GENETICA QUE CODIFICA PARA ESTA ENZIMA TAMBIEN HAN SIDO DETERMINADAS. SE DESCRIBE UNA ENZIMA PETN REDUCTASA CODIFICADA POR EL GEN ON. TAMBIEN SE DESCRIBE UN PROCEDIMIENTO PARA PRODUCIR LA ENZIMA PETN REDUCTASA EN GRANDES CANTIDADES, Y PROCEDIMIENTOS DE BIOREMEDIACION QUE UTILIZAN LA ENZIMA PRODUCIDA DE ESTA FORMA. TAMBIEN SE DESCRIBE UN PROCEDIMIENTO PARA DETECTAR LA PRESENCIA DE PETN EN UNA MUESTRA, JUNTO CON UN BIOSENSOR PARA SU UTILIZACION EN DICHO PROCEDMIENTO.
ES96922985T 1995-07-11 1996-07-08 Deteccion y biodegradacion de explosivos. Expired - Lifetime ES2189878T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9514138A GB2303136A (en) 1995-07-11 1995-07-11 Pentaerythritol tetranitrate reductase enzyme
GB9607220A GB2311784A (en) 1996-04-04 1996-04-04 Pentaerythritoltetranitrate(PETN) reductase and the expression thereof

Publications (1)

Publication Number Publication Date
ES2189878T3 true ES2189878T3 (es) 2003-07-16

Family

ID=26307371

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96922985T Expired - Lifetime ES2189878T3 (es) 1995-07-11 1996-07-08 Deteccion y biodegradacion de explosivos.

Country Status (12)

Country Link
US (1) US5928859A (es)
EP (1) EP0837941B1 (es)
AU (1) AU6365296A (es)
CA (1) CA2226729C (es)
CZ (1) CZ295656B6 (es)
DE (1) DE69626646T2 (es)
ES (1) ES2189878T3 (es)
HU (1) HU224000B1 (es)
IL (1) IL122801A (es)
NO (1) NO324210B1 (es)
PL (1) PL188502B1 (es)
WO (1) WO1997003201A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6334395B1 (en) * 1995-11-17 2002-01-01 The Ensign-Bickford Company Methods, apparatus, and systems for accelerated bioremediation of explosives
US6120627A (en) 1995-11-17 2000-09-19 The Ensign-Bickford Company Explosive with bioremediating capacity
GB2332432A (en) * 1997-12-19 1999-06-23 Secr Defence Biodegradation of Trinitrotoluene (TNT)
GB0328784D0 (en) * 2003-12-11 2004-01-14 Univ Wales Bangor Improvements in & relating to biosensors
DE102005007724A1 (de) 2005-02-18 2006-08-31 Heinrich Meurer Luftgestütztes Verfahren zur Exploration von Kohlenwasserstoffvorkommen
DE102005007723A1 (de) 2005-02-18 2006-08-24 Heinrich Meurer Luftgestütztes Verfahren zur Detektion von Landminen
US7673551B2 (en) 2007-08-15 2010-03-09 Heinrich Meurer Aerial-supported procedure for the detection of landmines
US7528949B2 (en) 2007-08-15 2009-05-05 Heinrich Meurer Aerial-supported procedure for exploration of hydrocarbon deposits
US8754284B2 (en) * 2008-02-01 2014-06-17 Orica Explosives Technology Pty Ltd Further improved blasting method
PL2305624T3 (pl) 2009-10-01 2018-05-30 Maxamcorp Holding, S.L. Samodegradowalne urządzenie wybuchowe
US8329455B2 (en) 2011-07-08 2012-12-11 Aikan North America, Inc. Systems and methods for digestion of solid waste
WO2019129506A1 (en) * 2017-12-31 2019-07-04 Dsm Ip Assets B.V. Denitrifying enzymes
CN113418940B (zh) * 2021-06-24 2023-03-14 电子科技大学 一种基于x射线示踪颗粒的检测方法及检测装置

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215919A (en) * 1991-02-25 1993-06-01 Takeda Chemical Industries, Ltd. Process for producing optically active 2-hydroxycycloalkanecarboxylic acid esters using microbially derived reductase
EP0511729B1 (en) * 1991-03-20 1996-05-15 Thermedics Detection Inc. Detection method for checking surfaces for specific compounds
AU6490494A (en) * 1993-03-22 1994-10-11 Utah State University Foundation Methods for the biodegradation of environmental pollutants
GB9410805D0 (en) * 1994-05-28 1994-07-20 British Nuclear Fuels Plc Biosensors

Also Published As

Publication number Publication date
EP0837941A1 (en) 1998-04-29
PL188502B1 (pl) 2005-02-28
NO980107L (no) 1998-03-11
CZ295656B6 (cs) 2005-09-14
PL324481A1 (en) 1998-05-25
NO324210B1 (no) 2007-09-10
NO980107D0 (no) 1998-01-09
AU6365296A (en) 1997-02-10
EP0837941B1 (en) 2003-03-12
HU224000B1 (hu) 2005-04-28
DE69626646T2 (de) 2003-12-18
IL122801A (en) 2002-08-14
DE69626646D1 (de) 2003-04-17
HUP9901395A3 (en) 2001-10-29
CA2226729A1 (en) 1997-01-30
WO1997003201A1 (en) 1997-01-30
US5928859A (en) 1999-07-27
CA2226729C (en) 2008-05-13
IL122801A0 (en) 1998-08-16
HUP9901395A2 (hu) 1999-08-30
CZ6698A3 (cs) 1998-08-12

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