ES2188335B1 - Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial. - Google Patents

Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial.

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Publication number
ES2188335B1
ES2188335B1 ES200002425A ES200002425A ES2188335B1 ES 2188335 B1 ES2188335 B1 ES 2188335B1 ES 200002425 A ES200002425 A ES 200002425A ES 200002425 A ES200002425 A ES 200002425A ES 2188335 B1 ES2188335 B1 ES 2188335B1
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ES
Spain
Prior art keywords
procedure
differentiation
dna
pcr amplification
euchariot
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200002425A
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English (en)
Other versions
ES2188335A1 (es
Inventor
Amparo Mercedes Querol Simon
M. Victoria Lopez Alonso
Eladio Barrio
M. Teresa Fernandez-Espinar Garcia
Daniel Ramon Vidal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
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Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200002425A priority Critical patent/ES2188335B1/es
Publication of ES2188335A1 publication Critical patent/ES2188335A1/es
Application granted granted Critical
Publication of ES2188335B1 publication Critical patent/ES2188335B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procedimiento para la diferenciación de microorganismos eucariotas basado en la amplificación por PCR de zonas variables del DNA mitocondrial. Esta invención propone un procedimiento para la identificación y caracterización de cepas de microorganismos eucariotas (levaduras y hongos filamentosos) de relevancia clínica o industrial. El método empleado se basa en la amplificación por la técnica de PCR de zonas variables del DNA mitocondrial (mtDNA) utilizando como material de partida una preparación de DNA total, una colonia proveniente de una placa de cultivo o una muestra biológica (alimento, bebida, aislado clínico, etc). Los resultados obtenidos mediante su uso permiten diferenciar cepas de la misma especie.
ES200002425A 2000-10-06 2000-10-06 Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial. Expired - Fee Related ES2188335B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200002425A ES2188335B1 (es) 2000-10-06 2000-10-06 Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200002425A ES2188335B1 (es) 2000-10-06 2000-10-06 Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial.

Publications (2)

Publication Number Publication Date
ES2188335A1 ES2188335A1 (es) 2003-06-16
ES2188335B1 true ES2188335B1 (es) 2004-12-01

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ES200002425A Expired - Fee Related ES2188335B1 (es) 2000-10-06 2000-10-06 Procedimiento para la diferenciacion de microorganismos eucariotas basado en la amplificacion por pcr de zonas variables del dna mitocondrial.

Country Status (1)

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ES (1) ES2188335B1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW388772B (en) * 1996-09-09 2000-05-01 Ss Pharmaceutical Co Materials and methods for detecting fungi

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAKAGAWA, Y. et al. "Amplification and sequencing of mitochondrial cytochrano C oxidase subunit II gene for phylogenetic analysis of yeast". INSTITUTE FOR FERMENTATION RESEARCH COMMUNICATIONS, 1997, Nº 18, páginas 52-56. *

Also Published As

Publication number Publication date
ES2188335A1 (es) 2003-06-16

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