ES2170637A1 - Metodo y kit para la seleccion de formas de resistencia viables de parasitos enteropatogenos. - Google Patents
Metodo y kit para la seleccion de formas de resistencia viables de parasitos enteropatogenos.Info
- Publication number
- ES2170637A1 ES2170637A1 ES200000370A ES200000370A ES2170637A1 ES 2170637 A1 ES2170637 A1 ES 2170637A1 ES 200000370 A ES200000370 A ES 200000370A ES 200000370 A ES200000370 A ES 200000370A ES 2170637 A1 ES2170637 A1 ES 2170637A1
- Authority
- ES
- Spain
- Prior art keywords
- enteropathogenic
- selection
- biological samples
- parasites
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Consiste en el desarrollo de un método y kit para la aplicación del mismo, que permite la selección de quistes viables de protozoos y parásitos enteropatógenos a partir de muestras biológicas complejas, como muestras fecales, agua, lodos, fertilizantes orgánicos, tierra, alimentos o cualquier otra muestra que pueda contener quistes de dichos parásitos enteropatógenos. El método consiste en la separación de los componentes de la muestra en función de su densidad relativa en un gradiente de densidad hiperosmótico, compuesto por una sal disuelta en un tampón. La centrifugación de la muestra permite la migración de las partículas a través del gradiente, separándose sus componentes en función de su densidad relativa en dicho gradiente. Los organismos viables forman una banda que puede ser extraída para su caracterización o diagnóstico.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000370A ES2170637B1 (es) | 2000-02-17 | 2000-02-17 | Metodo y kit para la seleccion de formas de resistencia viables de parasitos enteropatogenos. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000370A ES2170637B1 (es) | 2000-02-17 | 2000-02-17 | Metodo y kit para la seleccion de formas de resistencia viables de parasitos enteropatogenos. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2170637A1 true ES2170637A1 (es) | 2002-08-01 |
| ES2170637B1 ES2170637B1 (es) | 2004-01-01 |
Family
ID=8492348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200000370A Expired - Fee Related ES2170637B1 (es) | 2000-02-17 | 2000-02-17 | Metodo y kit para la seleccion de formas de resistencia viables de parasitos enteropatogenos. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2170637B1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2382516A1 (es) * | 2010-11-15 | 2012-06-11 | Consejo Superior De Investigaciones Científicas (Csic) | Método y sistema asociado para la detección y el análisis de agentes patógenos y/o capaces de causar el deterioro en alimentos vegetales. |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5334509A (en) * | 1992-10-22 | 1994-08-02 | Riordan Neil H | Method for detecting intestinal pathogen dientamoeba fragilis |
| US5789190A (en) * | 1996-10-09 | 1998-08-04 | Immucell Corporation | Methods and kit for the detection of cryptosporidium oocysts and giardia cysts |
-
2000
- 2000-02-17 ES ES200000370A patent/ES2170637B1/es not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5334509A (en) * | 1992-10-22 | 1994-08-02 | Riordan Neil H | Method for detecting intestinal pathogen dientamoeba fragilis |
| US5789190A (en) * | 1996-10-09 | 1998-08-04 | Immucell Corporation | Methods and kit for the detection of cryptosporidium oocysts and giardia cysts |
Non-Patent Citations (1)
| Title |
|---|
| EMILIO ENTRALA et al. Cryptosporidium Parvum: Oocysts Purification Using Potassium Bromide Discontinuous Gradient. Veterinary Parasitology, 92 (200), 223-226. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2382516A1 (es) * | 2010-11-15 | 2012-06-11 | Consejo Superior De Investigaciones Científicas (Csic) | Método y sistema asociado para la detección y el análisis de agentes patógenos y/o capaces de causar el deterioro en alimentos vegetales. |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2170637B1 (es) | 2004-01-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
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