ES2159333T3 - Metodos de ensayo analitico mejorados en quimica de capsulas utilizando quimiluminiscencia de dioxetano. - Google Patents

Metodos de ensayo analitico mejorados en quimica de capsulas utilizando quimiluminiscencia de dioxetano.

Info

Publication number
ES2159333T3
ES2159333T3 ES96112385T ES96112385T ES2159333T3 ES 2159333 T3 ES2159333 T3 ES 2159333T3 ES 96112385 T ES96112385 T ES 96112385T ES 96112385 T ES96112385 T ES 96112385T ES 2159333 T3 ES2159333 T3 ES 2159333T3
Authority
ES
Spain
Prior art keywords
dioxetane
capsules
improved
dioxethane
chemiluminiscence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96112385T
Other languages
English (en)
Inventor
Robert H Adolfsen
Hashem Akhavan-Tafti
Silva Renuka De
Paul A Schaap
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Wayne State University
Bayer Corp
Original Assignee
Bayer AG
Wayne State University
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Wayne State University, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of ES2159333T3 publication Critical patent/ES2159333T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y10T436/118339—Automated chemical analysis with a continuously flowing sample or carrier stream with formation of a segmented stream

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

SE PROPORCIONAN METODOS DE ENSAYO MEJORADOS QUE UTILIZAN 1,2DIOXETANOS ESTABLES, ENCIMATICAMENTE ACCIONADOS QUIMILUMINISCENTES CON SOLUBILIDAD EN AGUA MEJORADA. LOS ENSAYOS SE REALIZAN MEDIANTE UN METODO DE ENSAYO ANALITICO QUIMICO DE UNA CAPSULA EN DONDE LAS CAPSULAS DE FLUIDO QUE CONSTAN DE SEGMENTOS ACUOSOS DISCRETOS QUE CONTIENEN UN REACTIVO DE DIOXETANO QUIMILUMINISCENTE O UN AGENTE ACTIVADOR SEPARADOS MEDIANTE UN FLUIDO AISLANTE BASADO EN ACEITE FLUYEN A TRAVES DE UN CONDUCTO, Y POSTERIORMENTE SON ESTIMULADOS PARA PRODUCIR LUZ. LAS MEJORAS CONSISTEN EN EL USO DE UN DIOXETANO SUBSTITUIDO CON DOS O MAS GRUPOS SOLUBLES EN AGUA DISPUESTOS SOBRE LA ESTRUCTURA DE DIOXETANO PARA PROPORCIONAR LA QUIMILUMINISCENCIA. LAS COMPOSICIONES CONSTAN DE TAL DIOXETANO, UN REALZADOR SURFACTANTE NO POLIMERICO Y OPCIONALMENTE UN FLUORECEDOR, QUE PROPORCIONA QUIMILUMINISCENCIA MEJORADA Y ELIMINA EL PROBLEMA DEL REACTIVO EN EXCESO CUANDO SE USA EN ENSAYOS REALIZADOS SOBRE SISTEMAS ANALITICOS QUIMICOS DE CAPSULA.
ES96112385T 1995-07-31 1996-07-31 Metodos de ensayo analitico mejorados en quimica de capsulas utilizando quimiluminiscencia de dioxetano. Expired - Lifetime ES2159333T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/509,692 US5631167A (en) 1995-07-31 1995-07-31 Capsule chemistry analytical methods employing dioxetane chemiluminescence

Publications (1)

Publication Number Publication Date
ES2159333T3 true ES2159333T3 (es) 2001-10-01

Family

ID=24027708

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96112385T Expired - Lifetime ES2159333T3 (es) 1995-07-31 1996-07-31 Metodos de ensayo analitico mejorados en quimica de capsulas utilizando quimiluminiscencia de dioxetano.

Country Status (10)

Country Link
US (1) US5631167A (es)
EP (1) EP0757248B1 (es)
JP (1) JP3771642B2 (es)
AT (1) ATE201509T1 (es)
AU (1) AU719699B2 (es)
CA (1) CA2182326A1 (es)
DE (1) DE69612919T2 (es)
DK (1) DK0757248T3 (es)
ES (1) ES2159333T3 (es)
IL (1) IL118935A (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5721370A (en) * 1995-07-31 1998-02-24 Lumigen Inc. Water soluble tri-substituted 1,2-dioxetane compounds and assay compositions having increased storage stability
JP3182527B2 (ja) * 1996-09-03 2001-07-03 株式会社ヤトロン 化学発光の測定方法及びキット
US6623971B2 (en) * 1999-01-11 2003-09-23 Bayer Corporation Method and apparatus for conducting a stat immunoassay analysis in a capsule chemistry analysis system
WO2000073800A1 (fr) * 1999-06-01 2000-12-07 Kyokuto Pharmaceutical Industrial Co., Ltd. Methode et trousse de dosage d'anticorps anti-gad
US6767716B2 (en) * 2000-06-17 2004-07-27 Brij P. Giri Chemluminescent systems containing unsaturated 1,2-dioxetanes
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20060172425A1 (en) * 2005-02-01 2006-08-03 Bayer Healthcare, Llc Colored buffer solution for automated clinical analyzer

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4742010A (en) * 1986-05-23 1988-05-03 Syntex (U.S.A.) Inc. Assay method for the determination of lithium with novel substituted crown dyes
US5013827A (en) * 1986-07-17 1991-05-07 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US5068339A (en) * 1986-07-17 1991-11-26 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
US4962192A (en) * 1986-07-17 1990-10-09 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
US4959182A (en) * 1986-07-17 1990-09-25 Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
EP0275260B1 (en) * 1986-07-24 1993-11-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US5220005A (en) * 1986-07-24 1993-06-15 Tropix, Inc. Substituted adamantyl dioxetanes
CA1340590C (en) * 1986-07-24 1999-06-08 John C. Voyta Chemiluminescence enhancement
US5112960A (en) * 1989-07-17 1992-05-12 Bronstein Irena Y Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes
US5582980A (en) * 1989-07-17 1996-12-10 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
JPH0731201B2 (ja) * 1987-12-31 1995-04-10 トロピックス・インコーポレーテッド 化学発光による測定法
US4952707A (en) * 1988-06-30 1990-08-28 Tropix, Inc. Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
US5032381A (en) * 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
US5225584A (en) * 1989-09-06 1993-07-06 Tropix, Inc. Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor
US5547836A (en) * 1990-08-30 1996-08-20 Tropix, Inc. Enhancement of chemiluminescent assays
ES2038092T1 (es) * 1990-09-07 1993-07-16 The Board Of Governors Of Wayne State University Un compuesto de dioxetano.
US5248618A (en) * 1991-06-05 1993-09-28 Life Technologies, Inc. Methods for generating light with chemiluminescent dioxetanes activated by anchimeric assisted cleavage
US5268147A (en) * 1992-02-26 1993-12-07 Miles, Inc. Reversible direction capsule chemistry sample liquid analysis system and method
US5399497A (en) * 1992-02-26 1995-03-21 Miles, Inc. Capsule chemistry sample liquid analysis system and method
DE69229281T2 (de) * 1992-03-05 1999-09-23 Tropix Inc Synthese von stabilen, wasserlöslichen chemilumineszierenden 1,2-dioxetanen und ihre zwischenprodukte
US5393469A (en) * 1992-03-20 1995-02-28 Lumigen, Inc. Polymeric phosphonium salts providing enhanced chemiluminescence from 1,2-dioxetanes
US5603868A (en) * 1992-10-30 1997-02-18 Abbott Laboratories Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes
US5451347A (en) * 1993-06-24 1995-09-19 Lumigen, Inc. Methods and compositions providing enhanced chemiluminescence from chemiluminescent compounds using dicationic surfactants

Also Published As

Publication number Publication date
CA2182326A1 (en) 1997-02-01
JP3771642B2 (ja) 2006-04-26
AU6081796A (en) 1997-02-06
DE69612919D1 (de) 2001-06-28
EP0757248B1 (en) 2001-05-23
AU719699B2 (en) 2000-05-18
DE69612919T2 (de) 2002-04-11
DK0757248T3 (da) 2001-08-27
IL118935A0 (en) 1996-10-31
EP0757248A2 (en) 1997-02-05
EP0757248A3 (en) 1997-07-23
JPH09184810A (ja) 1997-07-15
IL118935A (en) 2000-11-21
ATE201509T1 (de) 2001-06-15
US5631167A (en) 1997-05-20

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