ES2158961T3 - Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. - Google Patents

Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.

Info

Publication number
ES2158961T3
ES2158961T3 ES95943256T ES95943256T ES2158961T3 ES 2158961 T3 ES2158961 T3 ES 2158961T3 ES 95943256 T ES95943256 T ES 95943256T ES 95943256 T ES95943256 T ES 95943256T ES 2158961 T3 ES2158961 T3 ES 2158961T3
Authority
ES
Spain
Prior art keywords
hapten
antibodies
pct
recognizing
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95943256T
Other languages
English (en)
Inventor
Philippe Cros
Nicole Battail
Nadia Piga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2158961T3 publication Critical patent/ES2158961T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA DOSIFICACION DE UN HAPTENO EN UNA MUESTRA, EN LA QUE SE PONE EN CONTACTO LA MUESTRA CON ANTICUERPOS CAPACES DE RECONOCER EL HAPTENO Y CON UNA CANTIDAD PREDETERMINADA DE UN REACTIVO CAPAZ DE ENTRAR EN COMPETICION CON DICHO HAPTENO PARA LA FIJACION SOBRE LOS CITADOS ANTICUERPOS. EL REACTIVO ES UN CONJUGADO FORMADO POR UN FRAGMENTO DE ACIDO NUCLEICO DE CORDON UNICO CON UN COMPUESTO CAPAZ DE SER RECONOCIDO POR LOS ANTICUERPOS QUE RECONOCEN EL HAPTENO. SE DETECTA DICHO REACTIVO, FIJADO O NO, MEDIANTE ANTICUERPOS CAPACES DE RECONOCER DICHO FRAGMENTO DE ACIDO NUCLEICO DEL CONJUGADO. LA DETECCION DE DICHO REACTIVO MEDIANTE ANTICUERPOS ANTIFRAGMENTO DE ACIDO NUCLEICO APORTA PARTICULARMENTE UNA MEJORA DE LA SENSIBILIDAD DE LA DOSIFICACION.
ES95943256T 1994-12-21 1995-12-21 Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. Expired - Lifetime ES2158961T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9415418A FR2728687A1 (fr) 1994-12-21 1994-12-21 Procede de dosage d'un haptene selon une technique de competition

Publications (1)

Publication Number Publication Date
ES2158961T3 true ES2158961T3 (es) 2001-09-16

Family

ID=9470068

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95943256T Expired - Lifetime ES2158961T3 (es) 1994-12-21 1995-12-21 Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.

Country Status (8)

Country Link
US (1) US5989831A (es)
EP (1) EP0799420B1 (es)
AT (1) ATE201769T1 (es)
CA (1) CA2208562A1 (es)
DE (1) DE69521145T2 (es)
ES (1) ES2158961T3 (es)
FR (1) FR2728687A1 (es)
WO (1) WO1996019729A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US20040038331A1 (en) * 2002-08-23 2004-02-26 Reddy M. Parameswara Solid phase synthesis of biomolecule conjugates
US20060035288A1 (en) * 2004-07-30 2006-02-16 Green Lawrence R Methods for detection of counterfeit liquids and foods
ES2717940T3 (es) * 2007-07-23 2019-06-26 Alere Tech Gmbh Ensayos
CN112946040B (zh) * 2021-02-04 2023-01-17 苏州大学 电化学发光免疫传感器及其在检测氟苯尼考中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824941A (en) * 1983-03-10 1989-04-25 Julian Gordon Specific antibody to the native form of 2'5'-oligonucleotides, the method of preparation and the use as reagents in immunoassays or for binding 2'5'-oligonucleotides in biological systems
US5656731A (en) * 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
US5429952A (en) * 1988-02-02 1995-07-04 Biocode, Inc. Marking of products to establish identity and source
US4921788A (en) * 1988-04-12 1990-05-01 The Research Foundation Of State University Of New York Competitive nucleic acid immunoassay for the detection of analytes
FR2663040B1 (fr) * 1990-06-11 1995-09-15 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
US5223393A (en) * 1990-06-12 1993-06-29 Microgenics Corporation Detection of analytes having binding sites for at least two binding moieties
AU3938893A (en) * 1992-03-27 1993-11-08 Abbott Laboratories Haptens, tracers, immunogens and antibodies for quinoline
WO1993020074A1 (en) * 1992-03-27 1993-10-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for acridine-9-carboxylic acids
DE4312399A1 (de) * 1993-04-16 1994-10-20 Boehringer Mannheim Gmbh Verfahren zur Bestimmung der Bindung eines Transkriptionsfaktors an eine Nukleinsäure
FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
US5648213A (en) * 1994-08-30 1997-07-15 Beckman Instruments, Inc. Compositions and methods for use in detection of analytes

Also Published As

Publication number Publication date
US5989831A (en) 1999-11-23
FR2728687A1 (fr) 1996-06-28
ATE201769T1 (de) 2001-06-15
FR2728687B1 (es) 1997-02-14
DE69521145T2 (de) 2002-03-07
EP0799420A1 (fr) 1997-10-08
WO1996019729A1 (fr) 1996-06-27
CA2208562A1 (fr) 1996-06-27
DE69521145D1 (de) 2001-07-05
EP0799420B1 (fr) 2001-05-30

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