ES2158961T3 - Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. - Google Patents

Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.

Info

Publication number
ES2158961T3
ES2158961T3 ES95943256T ES95943256T ES2158961T3 ES 2158961 T3 ES2158961 T3 ES 2158961T3 ES 95943256 T ES95943256 T ES 95943256T ES 95943256 T ES95943256 T ES 95943256T ES 2158961 T3 ES2158961 T3 ES 2158961T3
Authority
ES
Spain
Prior art keywords
hapten
antibodies
pct
recognizing
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95943256T
Other languages
English (en)
Inventor
Philippe Cros
Nicole Battail
Nadia Piga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2158961T3 publication Critical patent/ES2158961T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA DOSIFICACION DE UN HAPTENO EN UNA MUESTRA, EN LA QUE SE PONE EN CONTACTO LA MUESTRA CON ANTICUERPOS CAPACES DE RECONOCER EL HAPTENO Y CON UNA CANTIDAD PREDETERMINADA DE UN REACTIVO CAPAZ DE ENTRAR EN COMPETICION CON DICHO HAPTENO PARA LA FIJACION SOBRE LOS CITADOS ANTICUERPOS. EL REACTIVO ES UN CONJUGADO FORMADO POR UN FRAGMENTO DE ACIDO NUCLEICO DE CORDON UNICO CON UN COMPUESTO CAPAZ DE SER RECONOCIDO POR LOS ANTICUERPOS QUE RECONOCEN EL HAPTENO. SE DETECTA DICHO REACTIVO, FIJADO O NO, MEDIANTE ANTICUERPOS CAPACES DE RECONOCER DICHO FRAGMENTO DE ACIDO NUCLEICO DEL CONJUGADO. LA DETECCION DE DICHO REACTIVO MEDIANTE ANTICUERPOS ANTIFRAGMENTO DE ACIDO NUCLEICO APORTA PARTICULARMENTE UNA MEJORA DE LA SENSIBILIDAD DE LA DOSIFICACION.
ES95943256T 1994-12-21 1995-12-21 Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. Expired - Lifetime ES2158961T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9415418A FR2728687A1 (fr) 1994-12-21 1994-12-21 Procede de dosage d'un haptene selon une technique de competition

Publications (1)

Publication Number Publication Date
ES2158961T3 true ES2158961T3 (es) 2001-09-16

Family

ID=9470068

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95943256T Expired - Lifetime ES2158961T3 (es) 1994-12-21 1995-12-21 Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.

Country Status (8)

Country Link
US (1) US5989831A (es)
EP (1) EP0799420B1 (es)
AT (1) ATE201769T1 (es)
CA (1) CA2208562A1 (es)
DE (1) DE69521145T2 (es)
ES (1) ES2158961T3 (es)
FR (1) FR2728687A1 (es)
WO (1) WO1996019729A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US20040038331A1 (en) * 2002-08-23 2004-02-26 Reddy M. Parameswara Solid phase synthesis of biomolecule conjugates
US20060035288A1 (en) * 2004-07-30 2006-02-16 Green Lawrence R Methods for detection of counterfeit liquids and foods
NZ582786A (en) * 2007-07-23 2012-09-28 Clondiag Gmbh Assays for nucleic acids
CN112946040B (zh) * 2021-02-04 2023-01-17 苏州大学 电化学发光免疫传感器及其在检测氟苯尼考中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824941A (en) * 1983-03-10 1989-04-25 Julian Gordon Specific antibody to the native form of 2'5'-oligonucleotides, the method of preparation and the use as reagents in immunoassays or for binding 2'5'-oligonucleotides in biological systems
US5656731A (en) * 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
US5429952A (en) * 1988-02-02 1995-07-04 Biocode, Inc. Marking of products to establish identity and source
US4921788A (en) * 1988-04-12 1990-05-01 The Research Foundation Of State University Of New York Competitive nucleic acid immunoassay for the detection of analytes
FR2663040B1 (fr) * 1990-06-11 1995-09-15 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
US5223393A (en) * 1990-06-12 1993-06-29 Microgenics Corporation Detection of analytes having binding sites for at least two binding moieties
WO1993020074A1 (en) * 1992-03-27 1993-10-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for acridine-9-carboxylic acids
AU3938893A (en) * 1992-03-27 1993-11-08 Abbott Laboratories Haptens, tracers, immunogens and antibodies for quinoline
DE4312399A1 (de) * 1993-04-16 1994-10-20 Boehringer Mannheim Gmbh Verfahren zur Bestimmung der Bindung eines Transkriptionsfaktors an eine Nukleinsäure
FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
US5648213A (en) * 1994-08-30 1997-07-15 Beckman Instruments, Inc. Compositions and methods for use in detection of analytes

Also Published As

Publication number Publication date
DE69521145T2 (de) 2002-03-07
US5989831A (en) 1999-11-23
EP0799420B1 (fr) 2001-05-30
WO1996019729A1 (fr) 1996-06-27
DE69521145D1 (de) 2001-07-05
CA2208562A1 (fr) 1996-06-27
FR2728687B1 (es) 1997-02-14
FR2728687A1 (fr) 1996-06-28
ATE201769T1 (de) 2001-06-15
EP0799420A1 (fr) 1997-10-08

Similar Documents

Publication Publication Date Title
CA2081559A1 (en) Determination of analytes in biological fluids in the presence of substances interfering with assays therefor
DE69623458T2 (de) Einstufige immunochromatographische vorrichtung und verfahren zu ihrer verwendung
ATE201771T1 (de) Spezifische bindungstests und reagenzien dafür
ATE226729T1 (de) Quantitative immunochromatographische assays
DE3485920D1 (de) Kolorimetrisches immunoessay auf fester oberflaeche, sowie dazu zu verwendender teststab und seine herstellung.
ES2009939A6 (es) Un inmunoensayo para la deteccion simultanea de antigenos yno anticuerpos en una muestra a ensayar de un fluido fisiologico.
ES2022166B3 (es) Ensayo de enlace especifico heterogeneo que emplea un reactivo enlazante agregable.
GB9804083D0 (en) Immunoassays
FR2662804B1 (fr) Anticorps polyclonaux anti-lh et leurs applications a la detection et/ou au dosage de la lh, chez de multiples especes animales.
WO2001084146B1 (en) A sensitive, multiplexed mass-spectrometry-based biosensor based on immuno-pcr
NZ526300A (en) Detecting the presence of toxins (PSTs) in marine organisms by biochemical, physiological or chemical assays
ES2158961T3 (es) Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.
DK0755517T3 (da) Fremgangsmåde til detektion af tilstedeværelse af en Mycobacterium-art og et kit og antistoffer til anvendelse i denne
FR2598514B1 (fr) Procede de dosage immunologique des hormones thyroidiennes t3 et/ou t4 utilisant la thyroglobuline
DE69606024T2 (de) Feststellung von antikörperproduktion
ATE180894T1 (de) Verwendung von polyklonalen humanen anti-htg- autoantikörpern als reagenz für die klinische diagnostik von schilddrüsen-autoimmunerkrankungen sowie reagenziensatz für eine bestimmung von anti-htg-autoantikörpern in patientenseren
EP0848251A3 (en) Homogeneous on-line assays using capillary electrophoresis
De Waal Distribution, transmission and serodiagnosis of Babesia equi and Babesia caballi in South Africa.
WO2002014869A3 (en) Assay method and device for detecting the presence and concentration of analytes
Kolesnikova et al. Use of the ELISA to detect antibodies against Streptococcus group A polysaccharide in human sera.
Paeng et al. Quartz microtiter plate in homogeneous assay development
ATE208041T1 (de) A-protein als diagnostikum für krebs
DE69518182T2 (de) Methode zur Bestimmung einer Substanz

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 799420

Country of ref document: ES