ES2158961T3 - Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. - Google Patents
Procedimiento de dosificacion de un hapteno segun una tecnica de competicion.Info
- Publication number
- ES2158961T3 ES2158961T3 ES95943256T ES95943256T ES2158961T3 ES 2158961 T3 ES2158961 T3 ES 2158961T3 ES 95943256 T ES95943256 T ES 95943256T ES 95943256 T ES95943256 T ES 95943256T ES 2158961 T3 ES2158961 T3 ES 2158961T3
- Authority
- ES
- Spain
- Prior art keywords
- hapten
- antibodies
- pct
- recognizing
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Endocrinology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PROCEDIMIENTO PARA LA DOSIFICACION DE UN HAPTENO EN UNA MUESTRA, EN LA QUE SE PONE EN CONTACTO LA MUESTRA CON ANTICUERPOS CAPACES DE RECONOCER EL HAPTENO Y CON UNA CANTIDAD PREDETERMINADA DE UN REACTIVO CAPAZ DE ENTRAR EN COMPETICION CON DICHO HAPTENO PARA LA FIJACION SOBRE LOS CITADOS ANTICUERPOS. EL REACTIVO ES UN CONJUGADO FORMADO POR UN FRAGMENTO DE ACIDO NUCLEICO DE CORDON UNICO CON UN COMPUESTO CAPAZ DE SER RECONOCIDO POR LOS ANTICUERPOS QUE RECONOCEN EL HAPTENO. SE DETECTA DICHO REACTIVO, FIJADO O NO, MEDIANTE ANTICUERPOS CAPACES DE RECONOCER DICHO FRAGMENTO DE ACIDO NUCLEICO DEL CONJUGADO. LA DETECCION DE DICHO REACTIVO MEDIANTE ANTICUERPOS ANTIFRAGMENTO DE ACIDO NUCLEICO APORTA PARTICULARMENTE UNA MEJORA DE LA SENSIBILIDAD DE LA DOSIFICACION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9415418A FR2728687A1 (fr) | 1994-12-21 | 1994-12-21 | Procede de dosage d'un haptene selon une technique de competition |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2158961T3 true ES2158961T3 (es) | 2001-09-16 |
Family
ID=9470068
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95943256T Expired - Lifetime ES2158961T3 (es) | 1994-12-21 | 1995-12-21 | Procedimiento de dosificacion de un hapteno segun una tecnica de competicion. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5989831A (es) |
EP (1) | EP0799420B1 (es) |
AT (1) | ATE201769T1 (es) |
CA (1) | CA2208562A1 (es) |
DE (1) | DE69521145T2 (es) |
ES (1) | ES2158961T3 (es) |
FR (1) | FR2728687A1 (es) |
WO (1) | WO1996019729A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6902891B2 (en) | 1999-12-17 | 2005-06-07 | Bio Merieux | Process for labeling a nucleic acid |
US6489114B2 (en) | 1999-12-17 | 2002-12-03 | Bio Merieux | Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby |
US20040038331A1 (en) * | 2002-08-23 | 2004-02-26 | Reddy M. Parameswara | Solid phase synthesis of biomolecule conjugates |
US20060035288A1 (en) * | 2004-07-30 | 2006-02-16 | Green Lawrence R | Methods for detection of counterfeit liquids and foods |
ES2717940T3 (es) * | 2007-07-23 | 2019-06-26 | Alere Tech Gmbh | Ensayos |
CN112946040B (zh) * | 2021-02-04 | 2023-01-17 | 苏州大学 | 电化学发光免疫传感器及其在检测氟苯尼考中的应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4824941A (en) * | 1983-03-10 | 1989-04-25 | Julian Gordon | Specific antibody to the native form of 2'5'-oligonucleotides, the method of preparation and the use as reagents in immunoassays or for binding 2'5'-oligonucleotides in biological systems |
US5656731A (en) * | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
US5429952A (en) * | 1988-02-02 | 1995-07-04 | Biocode, Inc. | Marking of products to establish identity and source |
US4921788A (en) * | 1988-04-12 | 1990-05-01 | The Research Foundation Of State University Of New York | Competitive nucleic acid immunoassay for the detection of analytes |
FR2663040B1 (fr) * | 1990-06-11 | 1995-09-15 | Bio Merieux | Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich. |
US5223393A (en) * | 1990-06-12 | 1993-06-29 | Microgenics Corporation | Detection of analytes having binding sites for at least two binding moieties |
AU3938893A (en) * | 1992-03-27 | 1993-11-08 | Abbott Laboratories | Haptens, tracers, immunogens and antibodies for quinoline |
WO1993020074A1 (en) * | 1992-03-27 | 1993-10-14 | Abbott Laboratories | Haptens, tracers, immunogens and antibodies for acridine-9-carboxylic acids |
DE4312399A1 (de) * | 1993-04-16 | 1994-10-20 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung der Bindung eines Transkriptionsfaktors an eine Nukleinsäure |
FR2706618B1 (fr) * | 1993-06-11 | 1995-09-01 | Bio Merieux | Dispositif pour le dosage d'haptènes et son utilisation. |
US5648213A (en) * | 1994-08-30 | 1997-07-15 | Beckman Instruments, Inc. | Compositions and methods for use in detection of analytes |
-
1994
- 1994-12-21 FR FR9415418A patent/FR2728687A1/fr active Granted
-
1995
- 1995-12-21 US US08/860,298 patent/US5989831A/en not_active Expired - Fee Related
- 1995-12-21 WO PCT/FR1995/001709 patent/WO1996019729A1/fr active IP Right Grant
- 1995-12-21 DE DE69521145T patent/DE69521145T2/de not_active Expired - Fee Related
- 1995-12-21 EP EP95943256A patent/EP0799420B1/fr not_active Expired - Lifetime
- 1995-12-21 CA CA002208562A patent/CA2208562A1/fr not_active Abandoned
- 1995-12-21 ES ES95943256T patent/ES2158961T3/es not_active Expired - Lifetime
- 1995-12-21 AT AT95943256T patent/ATE201769T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
US5989831A (en) | 1999-11-23 |
FR2728687A1 (fr) | 1996-06-28 |
ATE201769T1 (de) | 2001-06-15 |
FR2728687B1 (es) | 1997-02-14 |
DE69521145T2 (de) | 2002-03-07 |
EP0799420A1 (fr) | 1997-10-08 |
WO1996019729A1 (fr) | 1996-06-27 |
CA2208562A1 (fr) | 1996-06-27 |
DE69521145D1 (de) | 2001-07-05 |
EP0799420B1 (fr) | 2001-05-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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