ES2156510B1 - A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. - Google Patents
A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED.Info
- Publication number
- ES2156510B1 ES2156510B1 ES9802352A ES9802352A ES2156510B1 ES 2156510 B1 ES2156510 B1 ES 2156510B1 ES 9802352 A ES9802352 A ES 9802352A ES 9802352 A ES9802352 A ES 9802352A ES 2156510 B1 ES2156510 B1 ES 2156510B1
- Authority
- ES
- Spain
- Prior art keywords
- pseudomonas
- mutants
- pseudomonas putida
- coli
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Un procedimiento para la producción de ácido 2- hidroxifenilacético utilizando mutantes de la bacteria Pseudomonas putida U así como una cepa recombinante de E.coli en la que se han introducido genes aislados de Pseudomonas putida. La invención que se presenta, describe un procedimiento para la biotransformación del ácido fenilacético (PA) en ácido 2- hidroxifenilacético (abreviadamente indicado como 2-HPA), utilizando mutantes de la bacteria Pseudomonas putida U interrumpidos en uno de los genes (phaL) que compone la ruta catabólica de ácido fenilacético, así como el empleo de una cepa de E.coli recombinante en la que se han introducido, y se expresan, los.genes aislados de Pseudomonas putida que codifican las enzimas responsables de esa biotransformación.A process for the production of 2-hydroxyphenylacetic acid using mutants of the bacterium Pseudomonas putida U as well as a recombinant strain of E.coli into which isolated genes of Pseudomonas putida have been introduced. The invention presented describes a process for the biotransformation of phenylacetic acid (PA) in 2-hydroxyphenylacetic acid (abbreviated as 2-HPA), using mutants of the bacterium Pseudomonas putida U interrupted in one of the genes (phaL) that composes the catabolic pathway of phenylacetic acid, as well as the use of a recombinant E.coli strain into which the isolated Pseudomonas putida genes that encode the enzymes responsible for that biotransformation have been introduced and expressed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9802352A ES2156510B1 (en) | 1998-11-11 | 1998-11-11 | A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9802352A ES2156510B1 (en) | 1998-11-11 | 1998-11-11 | A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2156510A1 ES2156510A1 (en) | 2001-06-16 |
ES2156510B1 true ES2156510B1 (en) | 2002-01-16 |
Family
ID=8305732
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9802352A Expired - Fee Related ES2156510B1 (en) | 1998-11-11 | 1998-11-11 | A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. |
Country Status (1)
Country | Link |
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ES (1) | ES2156510B1 (en) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4232522A1 (en) * | 1992-09-29 | 1994-03-31 | Basf Ag | Process for the fermentative production of 2-hydroxyphenylacetic acid |
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1998
- 1998-11-11 ES ES9802352A patent/ES2156510B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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ES2156510A1 (en) | 2001-06-16 |
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