ES2156510B1 - A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. - Google Patents

A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED.

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Publication number
ES2156510B1
ES2156510B1 ES9802352A ES9802352A ES2156510B1 ES 2156510 B1 ES2156510 B1 ES 2156510B1 ES 9802352 A ES9802352 A ES 9802352A ES 9802352 A ES9802352 A ES 9802352A ES 2156510 B1 ES2156510 B1 ES 2156510B1
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Spain
Prior art keywords
pseudomonas
mutants
pseudomonas putida
coli
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9802352A
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Spanish (es)
Other versions
ES2156510A1 (en
Inventor
Rodriguez Baltasar Minambres
Olivera Elias Rodriguez
Alonso Belen Garcia
Lopez Jose Luis Garcia
Barca Abel Ferrandez
Fernandez Eduardo Diaz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Leon
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Leon
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Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Universidad de Leon filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9802352A priority Critical patent/ES2156510B1/en
Publication of ES2156510A1 publication Critical patent/ES2156510A1/en
Application granted granted Critical
Publication of ES2156510B1 publication Critical patent/ES2156510B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento para la producción de ácido 2- hidroxifenilacético utilizando mutantes de la bacteria Pseudomonas putida U así como una cepa recombinante de E.coli en la que se han introducido genes aislados de Pseudomonas putida. La invención que se presenta, describe un procedimiento para la biotransformación del ácido fenilacético (PA) en ácido 2- hidroxifenilacético (abreviadamente indicado como 2-HPA), utilizando mutantes de la bacteria Pseudomonas putida U interrumpidos en uno de los genes (phaL) que compone la ruta catabólica de ácido fenilacético, así como el empleo de una cepa de E.coli recombinante en la que se han introducido, y se expresan, los.genes aislados de Pseudomonas putida que codifican las enzimas responsables de esa biotransformación.A process for the production of 2-hydroxyphenylacetic acid using mutants of the bacterium Pseudomonas putida U as well as a recombinant strain of E.coli into which isolated genes of Pseudomonas putida have been introduced. The invention presented describes a process for the biotransformation of phenylacetic acid (PA) in 2-hydroxyphenylacetic acid (abbreviated as 2-HPA), using mutants of the bacterium Pseudomonas putida U interrupted in one of the genes (phaL) that composes the catabolic pathway of phenylacetic acid, as well as the use of a recombinant E.coli strain into which the isolated Pseudomonas putida genes that encode the enzymes responsible for that biotransformation have been introduced and expressed.

ES9802352A 1998-11-11 1998-11-11 A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED. Expired - Fee Related ES2156510B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9802352A ES2156510B1 (en) 1998-11-11 1998-11-11 A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9802352A ES2156510B1 (en) 1998-11-11 1998-11-11 A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED.

Publications (2)

Publication Number Publication Date
ES2156510A1 ES2156510A1 (en) 2001-06-16
ES2156510B1 true ES2156510B1 (en) 2002-01-16

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Application Number Title Priority Date Filing Date
ES9802352A Expired - Fee Related ES2156510B1 (en) 1998-11-11 1998-11-11 A PROCEDURE FOR THE PRODUCTION OF 2-HYDROXYPHENYLACETIC ACID USING MUTANTS OF THE BATTERY PSEUDOMONAS PUTIDA OR ASI AS A RECOMBINATING CEPA OF E.COLI IN WHICH ISOLATED GENES OF PSEUDOMONAS HAVE BEEN INTRODUCED.

Country Status (1)

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ES (1) ES2156510B1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4232522A1 (en) * 1992-09-29 1994-03-31 Basf Ag Process for the fermentative production of 2-hydroxyphenylacetic acid

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Publication number Publication date
ES2156510A1 (en) 2001-06-16

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