ES2155379B1 - PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. - Google Patents
PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.Info
- Publication number
- ES2155379B1 ES2155379B1 ES9900666A ES9900666A ES2155379B1 ES 2155379 B1 ES2155379 B1 ES 2155379B1 ES 9900666 A ES9900666 A ES 9900666A ES 9900666 A ES9900666 A ES 9900666A ES 2155379 B1 ES2155379 B1 ES 2155379B1
- Authority
- ES
- Spain
- Prior art keywords
- cells
- procedure
- platelets
- culture
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Procedimiento para producir plaquetas humanas a partir de células en cultivo. Se describe un procedimiento para obtener cantidades ilimitadas de plaquetas humanas a partir de las líneas celulares K562 y KU812, derivadas de leucemias mieloides. El procedimiento se basa en el tratamiento con estaurosporina a concentraciones 50 a 100 nM durante 3 a 6 días. Las células pueden crecerse en suspensión y las plaquetas pueden ser separadas de las células por métodos estándar. Las plaquetas son reconocibles morfológicamente y comparten características bioquímicas y ultraestructurales con las plaquetas aisladas de sangre. Se puede obtener así una preparación de plaquetas humanas a partir de precursores celulares genéticamente idénticos y libre de otros productos biológicos de origen humano. Pueden ser usadas para fines analíticos como servir de estándar en ensayos plaquetarios biológicos o bioquímicos.Procedure to produce human platelets from cells in culture. A procedure is described for obtaining unlimited amounts of human platelets from cell lines K562 and KU812, derived from myeloid leukemias. The procedure is based on treatment with staurosporine at concentrations 50 to 100 nM for 3 to 6 days. Cells can be grown in suspension and platelets can be separated from cells by standard methods. Platelets are morphologically recognizable and share biochemical and ultrastructural characteristics with platelets isolated from blood. A preparation of human platelets can thus be obtained from genetically identical cell precursors and free from other biological products of human origin. They can be used for analytical purposes as a standard in biological or biochemical platelet assays.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9900666A ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9900666A ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2155379A1 ES2155379A1 (en) | 2001-05-01 |
ES2155379B1 true ES2155379B1 (en) | 2001-12-01 |
Family
ID=8307875
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9900666A Expired - Fee Related ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2155379B1 (en) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69326388T2 (en) * | 1992-06-22 | 1999-12-30 | Kyowa Hakko Kogyo Co., Ltd. | Process for the preparation of staurosporine derivatives |
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1999
- 1999-04-05 ES ES9900666A patent/ES2155379B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2155379A1 (en) | 2001-05-01 |
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Legal Events
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EC2A | Search report published |
Date of ref document: 20010501 Kind code of ref document: A1 Effective date: 20010501 |
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FD2A | Announcement of lapse in spain |
Effective date: 20190726 |