ES2155379B1 - PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. - Google Patents

PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

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Publication number
ES2155379B1
ES2155379B1 ES9900666A ES9900666A ES2155379B1 ES 2155379 B1 ES2155379 B1 ES 2155379B1 ES 9900666 A ES9900666 A ES 9900666A ES 9900666 A ES9900666 A ES 9900666A ES 2155379 B1 ES2155379 B1 ES 2155379B1
Authority
ES
Spain
Prior art keywords
cells
procedure
platelets
culture
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9900666A
Other languages
Spanish (es)
Other versions
ES2155379A1 (en
Inventor
Serrano Javier Leon
Flamarique Ana Lerga
Espiga Carlos Richard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Cantabria
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Universidad de Cantabria
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Cantabria filed Critical Universidad de Cantabria
Priority to ES9900666A priority Critical patent/ES2155379B1/en
Publication of ES2155379A1 publication Critical patent/ES2155379A1/en
Application granted granted Critical
Publication of ES2155379B1 publication Critical patent/ES2155379B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procedimiento para producir plaquetas humanas a partir de células en cultivo. Se describe un procedimiento para obtener cantidades ilimitadas de plaquetas humanas a partir de las líneas celulares K562 y KU812, derivadas de leucemias mieloides. El procedimiento se basa en el tratamiento con estaurosporina a concentraciones 50 a 100 nM durante 3 a 6 días. Las células pueden crecerse en suspensión y las plaquetas pueden ser separadas de las células por métodos estándar. Las plaquetas son reconocibles morfológicamente y comparten características bioquímicas y ultraestructurales con las plaquetas aisladas de sangre. Se puede obtener así una preparación de plaquetas humanas a partir de precursores celulares genéticamente idénticos y libre de otros productos biológicos de origen humano. Pueden ser usadas para fines analíticos como servir de estándar en ensayos plaquetarios biológicos o bioquímicos.Procedure to produce human platelets from cells in culture. A procedure is described for obtaining unlimited amounts of human platelets from cell lines K562 and KU812, derived from myeloid leukemias. The procedure is based on treatment with staurosporine at concentrations 50 to 100 nM for 3 to 6 days. Cells can be grown in suspension and platelets can be separated from cells by standard methods. Platelets are morphologically recognizable and share biochemical and ultrastructural characteristics with platelets isolated from blood. A preparation of human platelets can thus be obtained from genetically identical cell precursors and free from other biological products of human origin. They can be used for analytical purposes as a standard in biological or biochemical platelet assays.

ES9900666A 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. Expired - Fee Related ES2155379B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Publications (2)

Publication Number Publication Date
ES2155379A1 ES2155379A1 (en) 2001-05-01
ES2155379B1 true ES2155379B1 (en) 2001-12-01

Family

ID=8307875

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9900666A Expired - Fee Related ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Country Status (1)

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ES (1) ES2155379B1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69326388T2 (en) * 1992-06-22 1999-12-30 Kyowa Hakko Kogyo Co., Ltd. Process for the preparation of staurosporine derivatives

Also Published As

Publication number Publication date
ES2155379A1 (en) 2001-05-01

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