ES2155379A1 - Production of human plaquettes from culture cells consists of derivation of specific cell lines, for separation of plaquettes related to blood plaquettes - Google Patents
Production of human plaquettes from culture cells consists of derivation of specific cell lines, for separation of plaquettes related to blood plaquettesInfo
- Publication number
- ES2155379A1 ES2155379A1 ES9900666A ES9900666A ES2155379A1 ES 2155379 A1 ES2155379 A1 ES 2155379A1 ES 9900666 A ES9900666 A ES 9900666A ES 9900666 A ES9900666 A ES 9900666A ES 2155379 A1 ES2155379 A1 ES 2155379A1
- Authority
- ES
- Spain
- Prior art keywords
- plaquettes
- human
- production
- cell lines
- derivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The production of human plaquettes from culture cells comprises derivation of cell lines K562 and KU812 from myeloid leukemias. Treatment with staurosporin at 50-100 nM concentrations for 3-6 days enables the cells to grow in suspension, and the plaquettes can be separated from them in the standard manner.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9900666A ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9900666A ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2155379A1 true ES2155379A1 (en) | 2001-05-01 |
ES2155379B1 ES2155379B1 (en) | 2001-12-01 |
Family
ID=8307875
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9900666A Expired - Fee Related ES2155379B1 (en) | 1999-04-05 | 1999-04-05 | PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2155379B1 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2136103T3 (en) * | 1992-06-22 | 1999-11-16 | Kyowa Hakko Kogyo Kk | PROCEDURE FOR THE PREPARATION OF STAUROSPORIN DERIVATIVES. |
-
1999
- 1999-04-05 ES ES9900666A patent/ES2155379B1/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2136103T3 (en) * | 1992-06-22 | 1999-11-16 | Kyowa Hakko Kogyo Kk | PROCEDURE FOR THE PREPARATION OF STAUROSPORIN DERIVATIVES. |
Non-Patent Citations (3)
Title |
---|
ADACHI M. et al. "Induction of smg p21/rap1A p21/krev-1 p21 gen expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line". 1992. Oncogene. Vol. 7 (2), paginas 323-9. * |
MURGO A. et al. "K562 leukemia cells express P2T (adenosine diphosphate) purinergic receptors". 1992. J. Pharmacol. Exp. Ther., Vol. 261 (2), paginas 580-5. * |
YEN A. et al. "12-O-Tetradecanoylphorbol-13-acetate and staurosporine induce increased retinoblastoma tumor suppressor gene expression with megakaryocytic differentiation of leukemia cells". 1993. Cancer Res., Vol. 53 (13), paginas 3085-91. * |
Also Published As
Publication number | Publication date |
---|---|
ES2155379B1 (en) | 2001-12-01 |
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