ES2155379A1 - Production of human plaquettes from culture cells consists of derivation of specific cell lines, for separation of plaquettes related to blood plaquettes - Google Patents

Production of human plaquettes from culture cells consists of derivation of specific cell lines, for separation of plaquettes related to blood plaquettes

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Publication number
ES2155379A1
ES2155379A1 ES9900666A ES9900666A ES2155379A1 ES 2155379 A1 ES2155379 A1 ES 2155379A1 ES 9900666 A ES9900666 A ES 9900666A ES 9900666 A ES9900666 A ES 9900666A ES 2155379 A1 ES2155379 A1 ES 2155379A1
Authority
ES
Spain
Prior art keywords
plaquettes
human
production
cell lines
derivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9900666A
Other languages
Spanish (es)
Other versions
ES2155379B1 (en
Inventor
Serrano Javier Leon
Flamarique Ana Lerga
Espiga Carlos Richard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Cantabria
Original Assignee
Universidad de Cantabria
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Cantabria filed Critical Universidad de Cantabria
Priority to ES9900666A priority Critical patent/ES2155379B1/en
Publication of ES2155379A1 publication Critical patent/ES2155379A1/en
Application granted granted Critical
Publication of ES2155379B1 publication Critical patent/ES2155379B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The production of human plaquettes from culture cells comprises derivation of cell lines K562 and KU812 from myeloid leukemias. Treatment with staurosporin at 50-100 nM concentrations for 3-6 days enables the cells to grow in suspension, and the plaquettes can be separated from them in the standard manner.
ES9900666A 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE. Expired - Fee Related ES2155379B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Publications (2)

Publication Number Publication Date
ES2155379A1 true ES2155379A1 (en) 2001-05-01
ES2155379B1 ES2155379B1 (en) 2001-12-01

Family

ID=8307875

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9900666A Expired - Fee Related ES2155379B1 (en) 1999-04-05 1999-04-05 PROCEDURE TO PRODUCE HUMAN PLATES PUT FROM CELLS IN CULTURE.

Country Status (1)

Country Link
ES (1) ES2155379B1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2136103T3 (en) * 1992-06-22 1999-11-16 Kyowa Hakko Kogyo Kk PROCEDURE FOR THE PREPARATION OF STAUROSPORIN DERIVATIVES.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2136103T3 (en) * 1992-06-22 1999-11-16 Kyowa Hakko Kogyo Kk PROCEDURE FOR THE PREPARATION OF STAUROSPORIN DERIVATIVES.

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ADACHI M. et al. "Induction of smg p21/rap1A p21/krev-1 p21 gen expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line". 1992. Oncogene. Vol. 7 (2), paginas 323-9. *
MURGO A. et al. "K562 leukemia cells express P2T (adenosine diphosphate) purinergic receptors". 1992. J. Pharmacol. Exp. Ther., Vol. 261 (2), paginas 580-5. *
YEN A. et al. "12-O-Tetradecanoylphorbol-13-acetate and staurosporine induce increased retinoblastoma tumor suppressor gene expression with megakaryocytic differentiation of leukemia cells". 1993. Cancer Res., Vol. 53 (13), paginas 3085-91. *

Also Published As

Publication number Publication date
ES2155379B1 (en) 2001-12-01

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