ES2139761T3 - Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union. - Google Patents

Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union.

Info

Publication number
ES2139761T3
ES2139761T3 ES94931655T ES94931655T ES2139761T3 ES 2139761 T3 ES2139761 T3 ES 2139761T3 ES 94931655 T ES94931655 T ES 94931655T ES 94931655 T ES94931655 T ES 94931655T ES 2139761 T3 ES2139761 T3 ES 2139761T3
Authority
ES
Spain
Prior art keywords
polypeptide
binding
ligand
molecules
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94931655T
Other languages
English (en)
Inventor
Dario Neri
Gregory Paul Winter
Lalla Claudia Dibit De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939322772A external-priority patent/GB9322772D0/en
Application filed by Medical Research Council filed Critical Medical Research Council
Application granted granted Critical
Publication of ES2139761T3 publication Critical patent/ES2139761T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43568—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from wasps
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Insects & Arthropods (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LAS MOLECULAS COMPRENDEN: I) UN POLIPEPTIDO (TAL COMO CALMODULINA) QUE TIENE UNA AFINIDAD DE UNION DEPENDIENTE DEL CALCIO PARA UN LIGANTE Y II) OTRO POLIPEPTIDO, LOS POLIPEPTIDOS ESTAN PREFERIBLEMENTE UNIDOS POR UN ENLACE DE PEPTIDO Y SE PRODUCEN MEDIANTE EXPRESION RECOMBINANTE A PARTIR DE UNA FUSION DE GENES. LAS MOLECULAS SON UTILES EN LA DETECCION, INMOVILIZACION, OBJETIVACION Y PURIFICACION, ETIQUETADO DE CELULAS, Y ENSAYOS DE DESPLAZAMIENTO DE BANDA PARA DETERMINAR LA UNION DE UN MIEMBRO DE UN PAR DE UNION ESPECIFICO (SBP) PARA UN MIEMBRO SBP COMPLEMENTARIO. PARA PROPOSITOS DE ENSAYOS DE DESPLAZAMIENTO DE BANDA EL POLIPEPTIDO I) NO NECESITA TENER AFINIDAD DE UNION DEPENDIENTE DEL CALCIO PARA UN LIGANTE, PERO DEBERIA TENER UNA DISOCIACION CONSTANTE PARA UN LIGANTE DE 10 NM O MENOR, MEDIDA A UN PH DE ENTRE 6 Y 9, A 20 (GRADOS) C. EN UNA CONFORMACION ALTERNATIVA, UN POLIPEPTIDO DE UNION DE LA CALMODULINA QUE ES, O SE DERIVA DEL METASPORAN, SE UNE, COMO POLIPEPTIDO I) EN VEZ DE UN POLIPEPTIDO DE UNION, AL OTRO POLIPEPTIDO.
ES94931655T 1993-11-04 1994-11-04 Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union. Expired - Lifetime ES2139761T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939322772A GB9322772D0 (en) 1993-11-04 1993-11-04 Tagging
GB9405927A GB9405927D0 (en) 1993-11-04 1994-03-25 Tagging

Publications (1)

Publication Number Publication Date
ES2139761T3 true ES2139761T3 (es) 2000-02-16

Family

ID=26303796

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94931655T Expired - Lifetime ES2139761T3 (es) 1993-11-04 1994-11-04 Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union.

Country Status (10)

Country Link
EP (1) EP0726951B1 (es)
JP (1) JPH09504694A (es)
AT (1) ATE185373T1 (es)
AU (1) AU695107B2 (es)
CA (1) CA2175209A1 (es)
DE (1) DE69421086T2 (es)
DK (1) DK0726951T3 (es)
ES (1) ES2139761T3 (es)
GR (1) GR3031943T3 (es)
WO (1) WO1995012672A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2667197A (en) * 1996-04-16 1997-11-07 University Of Miami Stabilized preparations of human troponins and modifications thereof, diagnostic assay methods and assay kits
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747334A (en) * 1990-02-15 1998-05-05 The University Of North Carolina At Chapel Hill Random peptide library

Also Published As

Publication number Publication date
WO1995012672A1 (en) 1995-05-11
EP0726951A1 (en) 1996-08-21
JPH09504694A (ja) 1997-05-13
DE69421086T2 (de) 2000-03-02
AU8065494A (en) 1995-05-23
EP0726951B1 (en) 1999-10-06
DK0726951T3 (da) 2000-03-27
GR3031943T3 (en) 2000-03-31
AU695107B2 (en) 1998-08-06
ATE185373T1 (de) 1999-10-15
DE69421086D1 (de) 1999-11-11
CA2175209A1 (en) 1995-05-11

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