ES2137115B1 - Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. - Google Patents

Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.

Info

Publication number
ES2137115B1
ES2137115B1 ES9701759A ES9701759A ES2137115B1 ES 2137115 B1 ES2137115 B1 ES 2137115B1 ES 9701759 A ES9701759 A ES 9701759A ES 9701759 A ES9701759 A ES 9701759A ES 2137115 B1 ES2137115 B1 ES 2137115B1
Authority
ES
Spain
Prior art keywords
galactosidase
chromatographic step
single chromatographic
thermus
purify
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9701759A
Other languages
English (en)
Other versions
ES2137115A1 (es
Inventor
Herrero Alejandro Vian
Santiago Alfonso Carrascosa
Lopez Jose Luis Garcia
Rubio Estrella Cortes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9701759A priority Critical patent/ES2137115B1/es
Publication of ES2137115A1 publication Critical patent/ES2137115A1/es
Application granted granted Critical
Publication of ES2137115B1 publication Critical patent/ES2137115B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento para producir {b -Galactosidasa recombinante de Themus SP. (Cepa T2) en células hospedantes, y purificarla en un solo paso cromatográfico. Secuencia de nucleótidos inédita que corresponde a un gen de Thermus sp. (Cepa T2: ATCC 27737) y que codifica para una bota-galactosidasa termoestable. Obtención de construcciones genéticas para la expresión a alto nivel de dicha secuencia de nucleótidos en células de Eacherichia coli mediante su clonación en vectores de expresión. Purificación de una proteína híbrida con actividad beta-galactosidasa termoestable producida en E. coli en un sólo paso cromatográfico. Esta proteína quimérica termoestable tiene aplicación en hidrólisis de leche y productos lácteos, reducción del contenido en lactosa en los vertidos de sueros de lechería, marcaje frío de sondas de ácidos nucléicos por conjugación con los mismos, síntesis de oligosacáridos por transglicosilación y evaluación rápida de tratamientos térmicos moderados.
ES9701759A 1997-08-07 1997-08-07 Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. Expired - Fee Related ES2137115B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9701759A ES2137115B1 (es) 1997-08-07 1997-08-07 Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9701759A ES2137115B1 (es) 1997-08-07 1997-08-07 Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.

Publications (2)

Publication Number Publication Date
ES2137115A1 ES2137115A1 (es) 1999-12-01
ES2137115B1 true ES2137115B1 (es) 2000-08-16

Family

ID=8300355

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9701759A Expired - Fee Related ES2137115B1 (es) 1997-08-07 1997-08-07 Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.

Country Status (1)

Country Link
ES (1) ES2137115B1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2172380B1 (es) * 2000-03-03 2003-12-01 Consejo Superior Investigacion Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0761265B2 (ja) * 1987-02-09 1995-07-05 新技術事業団 サ−マス属に属する新菌株、新規なβ−ガラクトシダ−ゼ及びその製造法

Also Published As

Publication number Publication date
ES2137115A1 (es) 1999-12-01

Similar Documents

Publication Publication Date Title
Lin et al. Precursor sequence, processing, and urothelium-specific expression of a major 15-kDa protein subunit of asymmetric unit membrane.
Clarke et al. Cloning and expression of the β-N-acetylglucosaminidase gene from Streptococcus pneumoniae: generation of truncated enzymes with modified aglycon specificity
Zhang et al. Isomaltulose synthase from Klebsiella sp. strain LX3: gene cloning and characterization and engineering of thermostability
Buneva et al. Catalytic DNA-and RNA-hydrolyzing antibodies from milk of healthy human mothers
ATE290068T1 (de) Beta 1-4 n-acetylglucosaminyltransferase sowie das für diese kodierende gen
GB2320716B (en) Improvements in or relating to starch content of plants
Holley Structure of an alanine transfer ribonucleic acid
NO20014716L (no) Ny klasse enzymer i den biosyntetiske vei for produksjon av triacylglycerol og rekombinante DNA-molekyler som koder for disse
FERRERO‐GARCÍA et al. The action of Trypanosoma cruzi trans‐sialidase on glycolipids and glycoproteins
Rahfeld et al. Prospecting for microbial α-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase
Jespersgaard et al. Functional and immunogenic characterization of two cloned regions of Streptococcus mutans glucosyltransferase I
ATE450603T1 (de) Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren
WO1995006735A3 (en) Nucleotide sequences for novel protein tyrosine phosphatases
DE69535696D1 (de) Tripeptidyl-aminopeptidase
Fincher et al. Evidence for a loop mechanism of protein transport by the thylakoid Delta pH pathway
WO2000052136A3 (en) Human glycosylation enzymes
JP4784224B2 (ja) 糖鎖転移方法および糖鎖転移酵素
ES2137115B1 (es) Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.
ATE386130T1 (de) Adenovirale vektoren für die gentherapie
ES2126262T3 (es) Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe.
Ciampi et al. Biosynthesis of pseudouridine in the in vitro transcribed tRNATyr precursor
Sanchez-Beato et al. Tracking the evolution of the bacterial choline-binding domain: molecular characterization of the Clostridium acetobutylicum NCIB 8052 cspA gene
Mauch et al. Characterization of a linear epitope within the human pancreatic 64‐kDa glutamic acid decarboxylase and its autoimmune recognition by sera from insulin‐dependent diabetes mellitus patients
Bradshaw Jr et al. Nucleotide sequence of the traI (helicase I) gene from the sex factor F
Zheleznaya et al. Regulatory C Protein of the Eco RV Modification–Restriction System

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 19991201

Kind code of ref document: A1

Effective date: 19991201

FD2A Announcement of lapse in spain

Effective date: 20180807