ES2137115B1 - Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. - Google Patents
Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico.Info
- Publication number
- ES2137115B1 ES2137115B1 ES9701759A ES9701759A ES2137115B1 ES 2137115 B1 ES2137115 B1 ES 2137115B1 ES 9701759 A ES9701759 A ES 9701759A ES 9701759 A ES9701759 A ES 9701759A ES 2137115 B1 ES2137115 B1 ES 2137115B1
- Authority
- ES
- Spain
- Prior art keywords
- galactosidase
- chromatographic step
- single chromatographic
- thermus
- purify
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Un procedimiento para producir {b -Galactosidasa recombinante de Themus SP. (Cepa T2) en células hospedantes, y purificarla en un solo paso cromatográfico. Secuencia de nucleótidos inédita que corresponde a un gen de Thermus sp. (Cepa T2: ATCC 27737) y que codifica para una bota-galactosidasa termoestable. Obtención de construcciones genéticas para la expresión a alto nivel de dicha secuencia de nucleótidos en células de Eacherichia coli mediante su clonación en vectores de expresión. Purificación de una proteína híbrida con actividad beta-galactosidasa termoestable producida en E. coli en un sólo paso cromatográfico. Esta proteína quimérica termoestable tiene aplicación en hidrólisis de leche y productos lácteos, reducción del contenido en lactosa en los vertidos de sueros de lechería, marcaje frío de sondas de ácidos nucléicos por conjugación con los mismos, síntesis de oligosacáridos por transglicosilación y evaluación rápida de tratamientos térmicos moderados.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701759A ES2137115B1 (es) | 1997-08-07 | 1997-08-07 | Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701759A ES2137115B1 (es) | 1997-08-07 | 1997-08-07 | Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2137115A1 ES2137115A1 (es) | 1999-12-01 |
ES2137115B1 true ES2137115B1 (es) | 2000-08-16 |
Family
ID=8300355
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9701759A Expired - Fee Related ES2137115B1 (es) | 1997-08-07 | 1997-08-07 | Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2137115B1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2172380B1 (es) * | 2000-03-03 | 2003-12-01 | Consejo Superior Investigacion | Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes. |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0761265B2 (ja) * | 1987-02-09 | 1995-07-05 | 新技術事業団 | サ−マス属に属する新菌株、新規なβ−ガラクトシダ−ゼ及びその製造法 |
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1997
- 1997-08-07 ES ES9701759A patent/ES2137115B1/es not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2137115A1 (es) | 1999-12-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19991201 Kind code of ref document: A1 Effective date: 19991201 |
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FD2A | Announcement of lapse in spain |
Effective date: 20180807 |