ES2134174B1 - PROCEDURE FOR THE PRODUCTION OF ENTOMOPATHOGEN FUNGI FROM THE USE OF THE ALMOND MESOCARD. - Google Patents
PROCEDURE FOR THE PRODUCTION OF ENTOMOPATHOGEN FUNGI FROM THE USE OF THE ALMOND MESOCARD.Info
- Publication number
- ES2134174B1 ES2134174B1 ES9800174A ES9800174A ES2134174B1 ES 2134174 B1 ES2134174 B1 ES 2134174B1 ES 9800174 A ES9800174 A ES 9800174A ES 9800174 A ES9800174 A ES 9800174A ES 2134174 B1 ES2134174 B1 ES 2134174B1
- Authority
- ES
- Spain
- Prior art keywords
- almond
- procedure
- production
- mesocarp
- conidia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
Procedimiento para la producción de hongos entomopatógenos a partir de la utilización del mesocarpo de la almendra, que consiste en la utilización de un hongo denominado Verticillium lecanii inoculado sobre agar extracto de maíz, dispuesto sobre una placa Petri estéril de 9 centímetros de diámetro, utilizándose colonias de hongo aproximadamente siete días de crecimiento, estando el mesocarpo de la almendra secado al aire y cortado en fragmentos de 5x5 milímetros, el cual se sumerge a temperatura ambiente durante una hora en al agua destilada hasta alcanzar la saturación, para posteriormente ser esterilizado por calor húmero, mediante la autoclave de vapor durante 15 minutos a 120 ºC de temperatura, inoculándose 100 conidios diluidos en 5 mililitros de agua destilada estéril, produciéndose la germinación de los conidios a partir de los seis días, realizándose la operación de inoculado sobre una placa Petri.Procedure for the production of entomopathogenic fungi from the use of the almond mesocarp, which consists of the use of a fungus called Verticillium lecanii inoculated on corn extract agar, placed on a sterile Petri dish 9 centimeters in diameter, using colonies of mushroom approximately seven days of growth, being the almond mesocarp air-dried and cut into 5x5 millimeter fragments, which is immersed at room temperature for one hour in distilled water until saturation is reached, to be subsequently sterilized by heat humerus, by means of the steam autoclave for 15 minutes at a temperature of 120 ºC, inoculating 100 conidia diluted in 5 milliliters of sterile distilled water, producing the germination of the conidia from six days, performing the inoculation operation on a Petri dish .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9800174A ES2134174B1 (en) | 1998-01-30 | 1998-01-30 | PROCEDURE FOR THE PRODUCTION OF ENTOMOPATHOGEN FUNGI FROM THE USE OF THE ALMOND MESOCARD. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9800174A ES2134174B1 (en) | 1998-01-30 | 1998-01-30 | PROCEDURE FOR THE PRODUCTION OF ENTOMOPATHOGEN FUNGI FROM THE USE OF THE ALMOND MESOCARD. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2134174A1 ES2134174A1 (en) | 1999-09-16 |
| ES2134174B1 true ES2134174B1 (en) | 2000-05-01 |
Family
ID=8302572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES9800174A Expired - Fee Related ES2134174B1 (en) | 1998-01-30 | 1998-01-30 | PROCEDURE FOR THE PRODUCTION OF ENTOMOPATHOGEN FUNGI FROM THE USE OF THE ALMOND MESOCARD. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2134174B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2310134B1 (en) * | 2007-06-05 | 2010-01-07 | Universidad De Alicante | PHYTOSANITARY COMPOSITION THAT INCLUDES AN ENTOMOPATOGEN FUNCTION BELONGING TO SPECIES B. BASSIANA AND CRUSHED OR FRAGMENTED OF DATILES, METHOD OF ELABORATION AND USE OF THE SAME. |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU810180A1 (en) * | 1979-08-06 | 1981-03-07 | Научно-Исследовательская Лабораториякормовых Ресурсов Леса Всесоюзнойакдемии Сельскохозяйственных Науким.B.И.Ленина | Nutrient medium for cultivating beauveria bassianu(bals) vuill |
| JP2851506B2 (en) * | 1993-03-16 | 1999-01-27 | 静岡県 | Method for producing entomopathogenic fungi and medium for growing entomopathogenic fungi |
| MX9407877A (en) * | 1993-10-12 | 1997-02-28 | Clifford A Bradley | Solid culture substrate including barley. |
-
1998
- 1998-01-30 ES ES9800174A patent/ES2134174B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2134174A1 (en) | 1999-09-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 19990916 Kind code of ref document: A1 Effective date: 19990916 |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20180508 |