ES2133104A1 - Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. - Google Patents

Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2.

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Publication number
ES2133104A1
ES2133104A1 ES09701343A ES9701343A ES2133104A1 ES 2133104 A1 ES2133104 A1 ES 2133104A1 ES 09701343 A ES09701343 A ES 09701343A ES 9701343 A ES9701343 A ES 9701343A ES 2133104 A1 ES2133104 A1 ES 2133104A1
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Spain
Prior art keywords
gene
lys2
penicillin
chrysogenum
inactivation
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09701343A
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English (en)
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ES2133104B1 (es
Inventor
Javier Casqueiro
Martin Santiago Gutierrez
Oscar Banuelos
Fierro Francisco Fierro
Garcia Bruno Diez
De La Vieja Alfonso Collados
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Antibioticos SA
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Antibioticos SA
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Priority to ES9701343A priority Critical patent/ES2133104B1/es
Publication of ES2133104A1 publication Critical patent/ES2133104A1/es
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Publication of ES2133104B1 publication Critical patent/ES2133104B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un procedimiento para Incrementar la producción de penicilina en Penicillium chrysogenum mediante la inactivación del gen lys2. El gen lys2 de P. chrysogenum se ha identificado y aislado mediante hibridación con una sonda correspondiente al homólogo LYS2 de Saccharomyces cerevisiae. El gen de P. chrysogenum codifica una proteína de 1.296 aminoácidos y 142.421 Da con una identidad elevada con las reductasas de ácido a -aminoadípico de S. cerevisiae, Schizosaccharomyces pombe y Candida albicans. La disrupción del gen lys2 se ha realizado mediante (1) integración por recombinación simple y (II) sustitución génica por doble recombinación, empleando en ambos casos el gen pyrG como marcador de transformación. Los clones disrupcionados se han seleccionado como auxótrofos de lisina y confirmado por hibridación de Southern. Estos mutantes disrupcionados superproducen penicilina (40%-100% de Incremento con respecto a la cepa parental no disrupcionada) en un medio suplementado con L-lisinau4 mM. Figura 1.
ES9701343A 1997-06-19 1997-06-19 Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. Expired - Fee Related ES2133104B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9701343A ES2133104B1 (es) 1997-06-19 1997-06-19 Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9701343A ES2133104B1 (es) 1997-06-19 1997-06-19 Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2.

Publications (2)

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ES2133104A1 true ES2133104A1 (es) 1999-08-16
ES2133104B1 ES2133104B1 (es) 2000-04-01

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ES9701343A Expired - Fee Related ES2133104B1 (es) 1997-06-19 1997-06-19 Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2.

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1391510A1 (en) * 2001-05-29 2004-02-25 Asahi Glass Company Ltd. Method of constructing host and process for producing foreign protein

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARNES, D.A. et al. "Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene". MOLECULAR AND CELLULAR BIOLOGY, 1986, Vol. 6, No 8, paginas 2828-2838, *
EIBEL, H. et al. "Identification of the cloned S. cerevisiae LYS2 gene by an integrative transformation approach". MOL. GEN. GENET., 1983, Vol. 191, No 1, paginas 66-73 *
HöNLINGER, C. et al. "Differential effects of general amino acid control of lysine biosynthesis on penicillin formation in strains of Penicillium chrysogenum". THE JOURNAL OF ANTIBIOTICS, 1988, Vol. 41, No 2, paginas 255-257 *
LU, Y. et al. "Regulation of alpha-aminoadipate reductase from Penicillium chrysogenum in relation to the flux from alpha-aminoadipate into penicillin biosynthesis". CAN. J. MICROBIOL., 1992, Vol. 38, paginas 758-763 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1391510A1 (en) * 2001-05-29 2004-02-25 Asahi Glass Company Ltd. Method of constructing host and process for producing foreign protein
EP1391510A4 (en) * 2001-05-29 2005-06-01 Asahi Glass Co Ltd METHOD FOR CONSTRUCTING HOST AND METHOD FOR PRODUCING FOREIGN PROTEIN
US7723098B2 (en) 2001-05-29 2010-05-25 Asahi Glass Company, Limited Method of constructing host and method of producing heterologous protein

Also Published As

Publication number Publication date
ES2133104B1 (es) 2000-04-01

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