ES2129365A1 - Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. - Google Patents

Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.

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Publication number
ES2129365A1
ES2129365A1 ES09701600A ES9701600A ES2129365A1 ES 2129365 A1 ES2129365 A1 ES 2129365A1 ES 09701600 A ES09701600 A ES 09701600A ES 9701600 A ES9701600 A ES 9701600A ES 2129365 A1 ES2129365 A1 ES 2129365A1
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Spain
Prior art keywords
nucleic acid
acid sequences
internal control
specific nucleic
positive internal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09701600A
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English (en)
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ES2129365B1 (es
Inventor
Antonio Martinez
Juan Carlos Tercero
Emilio Valverde
Carmen Cabrero
Ana Diez
Amor Guerra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmagen S A
Original Assignee
Pharmagen S A
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Priority to ES009701600A priority Critical patent/ES2129365B1/es
Publication of ES2129365A1 publication Critical patent/ES2129365A1/es
Application granted granted Critical
Publication of ES2129365B1 publication Critical patent/ES2129365B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

Método para la detección de secuencias especificas de ácidos nucleidos en presencia de un vector útil como control positivo interno. El método comprende amplificar una secuencia específica presente en una muestra por medio de la reacción en cadena de la polimerasa en presencia de un vector control positivo interno que comprende la secuencia nucleótidica de al menos uno de los iniciadores utilizados en la PCR. El método es útil para la detección de secuencias específicas de ácido nucleico derivadas de microorganismos, células normales o cancerosas, virus RNA o DNA de cadena sencilla o doble o transcritos virales.
ES009701600A 1997-07-18 1997-07-18 Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. Expired - Fee Related ES2129365B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES009701600A ES2129365B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009701600A ES2129365B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.

Publications (2)

Publication Number Publication Date
ES2129365A1 true ES2129365A1 (es) 1999-06-01
ES2129365B1 ES2129365B1 (es) 2000-04-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES009701600A Expired - Fee Related ES2129365B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.

Country Status (1)

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ES (1) ES2129365B1 (es)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002817A1 (en) * 1989-08-21 1991-03-07 Cetus Corporation Quantitation of nucleic acids using the polymerase chain reaction
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
EP0528306A2 (en) * 1991-08-15 1993-02-24 F. Hoffmann-La Roche Ag Mycobacterium primers and probes
EP0586112A2 (en) * 1992-08-14 1994-03-09 Pharma Gen, S.A. Control of PCR mediated detection of micro-organisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002817A1 (en) * 1989-08-21 1991-03-07 Cetus Corporation Quantitation of nucleic acids using the polymerase chain reaction
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
EP0528306A2 (en) * 1991-08-15 1993-02-24 F. Hoffmann-La Roche Ag Mycobacterium primers and probes
EP0586112A2 (en) * 1992-08-14 1994-03-09 Pharma Gen, S.A. Control of PCR mediated detection of micro-organisms

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BLOCH, W. "A biochemical perspective of the polymerase chain reaction", BIOCHEMISTRY, 1991, Vol. 30, No 11, paginas 2735-2747, pagina 2742, columna derecha. *
PLIKATIS, B.B. et al.: "Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain assay". MOLECULAR AND CELLULAR PROBES, 1991, Vol. 5, paginas 215-219 *
URSI, J-P. et al. "Utility of an internal control for the polymerase chain reaction. Application to detection of Mycoplasma pneumoniae in clinical specimens", ACTA PATHOL. MICROBIOL. IMMUNOL. SCAND (APMIS), 1992, Vol. 100, paginas 635-639, *

Also Published As

Publication number Publication date
ES2129365B1 (es) 2000-04-01

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