ES2126521A1 - Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples - Google Patents
Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samplesInfo
- Publication number
- ES2126521A1 ES2126521A1 ES09701232A ES9701232A ES2126521A1 ES 2126521 A1 ES2126521 A1 ES 2126521A1 ES 09701232 A ES09701232 A ES 09701232A ES 9701232 A ES9701232 A ES 9701232A ES 2126521 A1 ES2126521 A1 ES 2126521A1
- Authority
- ES
- Spain
- Prior art keywords
- detection
- serum samples
- antibodies
- membrane
- red
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled and abnormal antibodies in serum samples. This method is divided into three stages, namely seeding, in which the corpuscles to be typed are seeded on an ink detector pad, detection buffer by centrifuging twice, at low and high revolutions, and detection: if the red corpuscles are negative, and the two layers are united only by the pad, the upper layer forms a clearly visible button if they are positive, and are well united by IgM antibodies or by human antiglobulin, it maintains its double-layer structure. The ink pad is a dense and viscous compound, with low ionic force permitting the red corpuscles to adhere to the base of the dish and is preferably composed of glycerine, citrate, polyethylene glycol and dextran. For application to the detection of antigens in the membranes of red corpuscles and antibodies in serum samples.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701232A ES2126521B1 (en) | 1997-06-05 | 1997-06-05 | METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701232A ES2126521B1 (en) | 1997-06-05 | 1997-06-05 | METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2126521A1 true ES2126521A1 (en) | 1999-03-16 |
ES2126521B1 ES2126521B1 (en) | 1999-11-16 |
Family
ID=8299589
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9701232A Expired - Fee Related ES2126521B1 (en) | 1997-06-05 | 1997-06-05 | METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2126521B1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004019038A3 (en) * | 2002-08-23 | 2004-04-22 | Deutsches Rotes Kreuz Blutspen | Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type |
ES2264403A1 (en) * | 2006-06-22 | 2006-12-16 | Grifols S.A. | Suspension medium for red blood cells |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4275053A (en) * | 1975-08-14 | 1981-06-23 | Mt. Sinai School Of Medicine Of The City University Of New York | Blood cell typing and compatibility test procedure |
EP0039195A2 (en) * | 1980-04-28 | 1981-11-04 | Montefiore Hospital and Medical Center | Antibody detection process |
EP0194212A1 (en) * | 1985-02-08 | 1986-09-10 | Fondation pour la recherche de diagnostiques de laboratoire | Method for detecting agglutinated erythrocytes |
EP0454509A2 (en) * | 1990-03-27 | 1991-10-30 | Centre Regional De Transfusion Sanguine De Lille | Method to reveal erythrocyte agglutination for the determination of blood compatybility |
-
1997
- 1997-06-05 ES ES9701232A patent/ES2126521B1/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4275053A (en) * | 1975-08-14 | 1981-06-23 | Mt. Sinai School Of Medicine Of The City University Of New York | Blood cell typing and compatibility test procedure |
EP0039195A2 (en) * | 1980-04-28 | 1981-11-04 | Montefiore Hospital and Medical Center | Antibody detection process |
EP0194212A1 (en) * | 1985-02-08 | 1986-09-10 | Fondation pour la recherche de diagnostiques de laboratoire | Method for detecting agglutinated erythrocytes |
EP0454509A2 (en) * | 1990-03-27 | 1991-10-30 | Centre Regional De Transfusion Sanguine De Lille | Method to reveal erythrocyte agglutination for the determination of blood compatybility |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004019038A3 (en) * | 2002-08-23 | 2004-04-22 | Deutsches Rotes Kreuz Blutspen | Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type |
ES2264403A1 (en) * | 2006-06-22 | 2006-12-16 | Grifols S.A. | Suspension medium for red blood cells |
US8802385B2 (en) | 2006-06-22 | 2014-08-12 | Grifols, S.A. | Suspension medium for red blood cells comprising amino acids |
Also Published As
Publication number | Publication date |
---|---|
ES2126521B1 (en) | 1999-11-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wahl et al. | Isolation of human mononuclear cell subsets by counterflow centrifugal elutriation (CCE): I. Characterization of B-lymphocyte-, T-lymphocyte-, and monocyte-enriched fractions by flow cytometric analysis | |
US5500375A (en) | Integrated packaging-holder device for immunochromatographic assays in flow-through or dipstick formats | |
EP0312394A3 (en) | Membrane-supported immunoassays | |
TW442660B (en) | Immunochromatographic assay device | |
DE3853588T2 (en) | MONOCLONAL ANTIBODY, SPECIFIC TO THE FUNCTIONAL ADHESIVE AREA OF THE SURFACE PROTEIN OF A PHAGOCYTE CELL. | |
AU6859696A (en) | Efficient enrichment and detection of disseminated tumor cells | |
AU7165987A (en) | In vitro assay for detecting cell-mediated immune responses | |
BR9103342A (en) | DEVICE FOR SEPARATING BY CENTRIFUGATION TWO PHASES OF A SAMPLE OF A HETEROGENEOUS LIQUID, USEFULLY FOR THE SEPARATION OF THE PLASMA FROM THE WHOLE BLOOD | |
ES2009788B3 (en) | METHOD OF IMPROVING THE DEMARCATION AND SEPARATION OF CELLS IN CENTRIFUGATED BLOOD SAMPLES. | |
JPH10132800A (en) | Specimen-protecting container integral with humor-separating sheet | |
ES2126521A1 (en) | Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples | |
CA2375448A1 (en) | Method for detecting syphilis using synthetic antigens | |
CN109342736A (en) | A kind of microtrabeculae agglutination antihuman globulin detection card | |
DK426588D0 (en) | PROCEDURE FOR DETECTING CELL SURFACE ANTIGEN | |
ATE140083T1 (en) | DEVICE FOR SEPARATING AND DETERMINING ERYTHROCYTE AGGLUTINATES IN ONE STEP | |
CN205015346U (en) | Be applied to infectiousness sample test's device | |
WO2003021255A1 (en) | Blood examination instrument | |
EP0299299A3 (en) | Diagnostic system employing a unitary substrate to immobilize microspheres | |
Cunningham et al. | Maternal alloantibody responses during early pregnancy detected by a cellular enzyme-linked immunospecific assay | |
CN2914090Y (en) | Colloidal gold test paper for rapidly detecting amphetamine | |
DE3670307D1 (en) | SPECIFIC MYCOPLASMA MEMBRANE ANTIGENS AND ANTIBODIES AND THEIR CLINICAL USE. | |
Tamura et al. | Heterophile antibodies in sera of patients with Chediak-Higashi syndrome | |
EP0328372A3 (en) | Method for determining antigen | |
Kang et al. | A study of Corelation between Rosette Positivity and Plasma Level of Immunoglobulins in the Pulmonary Tuberculosis Patients | |
Næss et al. | Infectious mononucleosis: increase of high-affinity E-receptor-bearing lymphocytes |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19990316 Kind code of ref document: A1 Effective date: 19990316 |
|
GC2A | Exploitation certificate registered application with search report |
Effective date: 20010604 |
|
FD1A | Patent lapsed |
Effective date: 20050606 |