ES2126521A1 - Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples - Google Patents

Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples

Info

Publication number
ES2126521A1
ES2126521A1 ES09701232A ES9701232A ES2126521A1 ES 2126521 A1 ES2126521 A1 ES 2126521A1 ES 09701232 A ES09701232 A ES 09701232A ES 9701232 A ES9701232 A ES 9701232A ES 2126521 A1 ES2126521 A1 ES 2126521A1
Authority
ES
Spain
Prior art keywords
detection
serum samples
antibodies
membrane
red
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09701232A
Other languages
Spanish (es)
Other versions
ES2126521B1 (en
Inventor
Lopez Francisco Llopis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TRANSFUSION de la COMUNIDAD VA
Original Assignee
TRANSFUSION de la COMUNIDAD VA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TRANSFUSION de la COMUNIDAD VA filed Critical TRANSFUSION de la COMUNIDAD VA
Priority to ES9701232A priority Critical patent/ES2126521B1/en
Publication of ES2126521A1 publication Critical patent/ES2126521A1/en
Application granted granted Critical
Publication of ES2126521B1 publication Critical patent/ES2126521B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled and abnormal antibodies in serum samples. This method is divided into three stages, namely seeding, in which the corpuscles to be typed are seeded on an ink detector pad, detection buffer by centrifuging twice, at low and high revolutions, and detection: if the red corpuscles are negative, and the two layers are united only by the pad, the upper layer forms a clearly visible button if they are positive, and are well united by IgM antibodies or by human antiglobulin, it maintains its double-layer structure. The ink pad is a dense and viscous compound, with low ionic force permitting the red corpuscles to adhere to the base of the dish and is preferably composed of glycerine, citrate, polyethylene glycol and dextran. For application to the detection of antigens in the membranes of red corpuscles and antibodies in serum samples.
ES9701232A 1997-06-05 1997-06-05 METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES. Expired - Fee Related ES2126521B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9701232A ES2126521B1 (en) 1997-06-05 1997-06-05 METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9701232A ES2126521B1 (en) 1997-06-05 1997-06-05 METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES.

Publications (2)

Publication Number Publication Date
ES2126521A1 true ES2126521A1 (en) 1999-03-16
ES2126521B1 ES2126521B1 (en) 1999-11-16

Family

ID=8299589

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9701232A Expired - Fee Related ES2126521B1 (en) 1997-06-05 1997-06-05 METHOD FOR THE DETECTION OF ANTIGENS PRESENT IN THE MEMBRANE OF HEMATIES, OWN OR COUPLED AND OF IRREGULAR ANTIBODIES IN SERUM SAMPLES.

Country Status (1)

Country Link
ES (1) ES2126521B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004019038A3 (en) * 2002-08-23 2004-04-22 Deutsches Rotes Kreuz Blutspen Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type
ES2264403A1 (en) * 2006-06-22 2006-12-16 Grifols S.A. Suspension medium for red blood cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275053A (en) * 1975-08-14 1981-06-23 Mt. Sinai School Of Medicine Of The City University Of New York Blood cell typing and compatibility test procedure
EP0039195A2 (en) * 1980-04-28 1981-11-04 Montefiore Hospital and Medical Center Antibody detection process
EP0194212A1 (en) * 1985-02-08 1986-09-10 Fondation pour la recherche de diagnostiques de laboratoire Method for detecting agglutinated erythrocytes
EP0454509A2 (en) * 1990-03-27 1991-10-30 Centre Regional De Transfusion Sanguine De Lille Method to reveal erythrocyte agglutination for the determination of blood compatybility

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275053A (en) * 1975-08-14 1981-06-23 Mt. Sinai School Of Medicine Of The City University Of New York Blood cell typing and compatibility test procedure
EP0039195A2 (en) * 1980-04-28 1981-11-04 Montefiore Hospital and Medical Center Antibody detection process
EP0194212A1 (en) * 1985-02-08 1986-09-10 Fondation pour la recherche de diagnostiques de laboratoire Method for detecting agglutinated erythrocytes
EP0454509A2 (en) * 1990-03-27 1991-10-30 Centre Regional De Transfusion Sanguine De Lille Method to reveal erythrocyte agglutination for the determination of blood compatybility

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004019038A3 (en) * 2002-08-23 2004-04-22 Deutsches Rotes Kreuz Blutspen Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type
ES2264403A1 (en) * 2006-06-22 2006-12-16 Grifols S.A. Suspension medium for red blood cells
US8802385B2 (en) 2006-06-22 2014-08-12 Grifols, S.A. Suspension medium for red blood cells comprising amino acids

Also Published As

Publication number Publication date
ES2126521B1 (en) 1999-11-16

Similar Documents

Publication Publication Date Title
Wahl et al. Isolation of human mononuclear cell subsets by counterflow centrifugal elutriation (CCE): I. Characterization of B-lymphocyte-, T-lymphocyte-, and monocyte-enriched fractions by flow cytometric analysis
US5500375A (en) Integrated packaging-holder device for immunochromatographic assays in flow-through or dipstick formats
EP0312394A3 (en) Membrane-supported immunoassays
TW442660B (en) Immunochromatographic assay device
DE3853588T2 (en) MONOCLONAL ANTIBODY, SPECIFIC TO THE FUNCTIONAL ADHESIVE AREA OF THE SURFACE PROTEIN OF A PHAGOCYTE CELL.
AU6859696A (en) Efficient enrichment and detection of disseminated tumor cells
AU7165987A (en) In vitro assay for detecting cell-mediated immune responses
BR9103342A (en) DEVICE FOR SEPARATING BY CENTRIFUGATION TWO PHASES OF A SAMPLE OF A HETEROGENEOUS LIQUID, USEFULLY FOR THE SEPARATION OF THE PLASMA FROM THE WHOLE BLOOD
ES2009788B3 (en) METHOD OF IMPROVING THE DEMARCATION AND SEPARATION OF CELLS IN CENTRIFUGATED BLOOD SAMPLES.
JPH10132800A (en) Specimen-protecting container integral with humor-separating sheet
ES2126521A1 (en) Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples
CA2375448A1 (en) Method for detecting syphilis using synthetic antigens
CN109342736A (en) A kind of microtrabeculae agglutination antihuman globulin detection card
DK426588D0 (en) PROCEDURE FOR DETECTING CELL SURFACE ANTIGEN
ATE140083T1 (en) DEVICE FOR SEPARATING AND DETERMINING ERYTHROCYTE AGGLUTINATES IN ONE STEP
CN205015346U (en) Be applied to infectiousness sample test's device
WO2003021255A1 (en) Blood examination instrument
EP0299299A3 (en) Diagnostic system employing a unitary substrate to immobilize microspheres
Cunningham et al. Maternal alloantibody responses during early pregnancy detected by a cellular enzyme-linked immunospecific assay
CN2914090Y (en) Colloidal gold test paper for rapidly detecting amphetamine
DE3670307D1 (en) SPECIFIC MYCOPLASMA MEMBRANE ANTIGENS AND ANTIBODIES AND THEIR CLINICAL USE.
Tamura et al. Heterophile antibodies in sera of patients with Chediak-Higashi syndrome
EP0328372A3 (en) Method for determining antigen
Kang et al. A study of Corelation between Rosette Positivity and Plasma Level of Immunoglobulins in the Pulmonary Tuberculosis Patients
Næss et al. Infectious mononucleosis: increase of high-affinity E-receptor-bearing lymphocytes

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 19990316

Kind code of ref document: A1

Effective date: 19990316

GC2A Exploitation certificate registered application with search report

Effective date: 20010604

FD1A Patent lapsed

Effective date: 20050606