EP0299299A3 - Diagnostic system employing a unitary substrate to immobilize microspheres - Google Patents

Diagnostic system employing a unitary substrate to immobilize microspheres Download PDF

Info

Publication number
EP0299299A3
EP0299299A3 EP19880110558 EP88110558A EP0299299A3 EP 0299299 A3 EP0299299 A3 EP 0299299A3 EP 19880110558 EP19880110558 EP 19880110558 EP 88110558 A EP88110558 A EP 88110558A EP 0299299 A3 EP0299299 A3 EP 0299299A3
Authority
EP
European Patent Office
Prior art keywords
substrate
microspheres
immobilize
diagnostic system
system employing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP19880110558
Other languages
German (de)
French (fr)
Other versions
EP0299299A2 (en
EP0299299B1 (en
Inventor
Michael W. Smith
Robert S. Pierce
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Porex Technologies Corp
Original Assignee
Porex Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Porex Technologies Corp filed Critical Porex Technologies Corp
Publication of EP0299299A2 publication Critical patent/EP0299299A2/en
Publication of EP0299299A3 publication Critical patent/EP0299299A3/en
Application granted granted Critical
Publication of EP0299299B1 publication Critical patent/EP0299299B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
    • C09J2203/00—Applications of adhesives in processes or use of adhesives in the form of films or foils
    • C09J2203/102—Applications of adhesives in processes or use of adhesives in the form of films or foils in the form of dowels, anchors or cartridges

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

In a diagnostic apparatus system, a one piece porous substrate (31) is provided in a container (33). The substrate serves both to extract an antigen in or on a top layer (36) thereof with the remainder of the substrate serving as a reservoir. The pores in the top surface (38) of the substrate are microscopic for entrapment of microspheres carrying antibodies. A target antigen in a test sample attaches to the antibodies when the test sample is poured through the top layer. The pores in all but the top layer of the substrate have a much greater pore size to comprise the reservoir portion of the substrate.
EP19880110558 1987-07-17 1988-07-01 Diagnostic system employing a unitary substrate to immobilize microspheres Expired - Lifetime EP0299299B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7496887A 1987-07-17 1987-07-17
US74968 1987-07-17

Publications (3)

Publication Number Publication Date
EP0299299A2 EP0299299A2 (en) 1989-01-18
EP0299299A3 true EP0299299A3 (en) 1991-03-13
EP0299299B1 EP0299299B1 (en) 1994-10-05

Family

ID=22122730

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19880110558 Expired - Lifetime EP0299299B1 (en) 1987-07-17 1988-07-01 Diagnostic system employing a unitary substrate to immobilize microspheres

Country Status (4)

Country Link
EP (1) EP0299299B1 (en)
JP (1) JPH01112160A (en)
CA (1) CA1309343C (en)
DE (1) DE3851725T2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393961A3 (en) * 1989-04-19 1991-10-30 Pall Corporation Porous, absorbent, multiple element diagnostic device
EP0394021A3 (en) * 1989-04-19 1991-11-21 Pall Corporation Diagnostic device with porous, absorbent single element
KR910014706A (en) * 1990-01-10 1991-08-31 원본미기재 Improved Immunoassay Device Without Cleaning
US5173260A (en) * 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
CA2038776A1 (en) * 1991-03-21 1992-09-22 Kevin Kearns Assay using an absorbent material
AU4925593A (en) * 1992-10-08 1994-05-09 Abbott Laboratories Assay devices using subsurface flow
EP0724909A1 (en) 1994-11-28 1996-08-07 Akzo Nobel N.V. Sample collection device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE711286A (en) * 1968-02-26 1968-07-01
FR2502508A1 (en) * 1981-03-30 1982-10-01 Geceral Grpt Etu Ceramiques Al Filter and membranes for ultrafiltration - employing coarse, and fine-grained sintered materials in combination to allow control of pore size
WO1985005451A1 (en) * 1984-05-11 1985-12-05 Hybritech Incorporated Method and apparatus for immunoassays
EP0207721A2 (en) * 1985-06-27 1987-01-07 A/G Technology Corporation Anisotropic membranes for gas separation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55132605A (en) * 1979-04-02 1980-10-15 Mitsubishi Rayon Co Ltd Preparation of semi-permeable membrane

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE711286A (en) * 1968-02-26 1968-07-01
FR2502508A1 (en) * 1981-03-30 1982-10-01 Geceral Grpt Etu Ceramiques Al Filter and membranes for ultrafiltration - employing coarse, and fine-grained sintered materials in combination to allow control of pore size
WO1985005451A1 (en) * 1984-05-11 1985-12-05 Hybritech Incorporated Method and apparatus for immunoassays
EP0207721A2 (en) * 1985-06-27 1987-01-07 A/G Technology Corporation Anisotropic membranes for gas separation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WPI/DERWENT, abstr.no. 80-85124C, & JP-A-55132605 (MITSUBISHI RAYON KK) *

Also Published As

Publication number Publication date
EP0299299A2 (en) 1989-01-18
DE3851725D1 (en) 1994-11-10
JPH01112160A (en) 1989-04-28
CA1309343C (en) 1992-10-27
DE3851725T2 (en) 1995-05-11
EP0299299B1 (en) 1994-10-05

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