ES2126513A1 - Process for detecting fungi and bacteria in air- conditioning ducts - Google Patents
Process for detecting fungi and bacteria in air- conditioning ductsInfo
- Publication number
- ES2126513A1 ES2126513A1 ES09700559A ES9700559A ES2126513A1 ES 2126513 A1 ES2126513 A1 ES 2126513A1 ES 09700559 A ES09700559 A ES 09700559A ES 9700559 A ES9700559 A ES 9700559A ES 2126513 A1 ES2126513 A1 ES 2126513A1
- Authority
- ES
- Spain
- Prior art keywords
- bacteria
- air
- conditioning ducts
- fungi
- detecting fungi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Process for detecting fungi and bacteria in air- conditioning ducts. Process for detecting fungi and bacteria used as sources for oligonucleotides with the following stages: a) sample collection using a swab and resuspension in distilled water, b) boiling of the sample, c) PCR reaction with the boiled sample in the presence of high concentrations of bovine serum albumin, d) analysis of the PCR reaction on agarose/etidium bromide gels using suitable controls. Through this method it is possible to detect the presence of fungi and/or bacteria in air-conditioning ducts rapidly, reliably and effectively.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES009700559A ES2126513B1 (en) | 1997-03-14 | 1997-03-14 | PROCEDURE FOR THE DETECTION OF FUNGI AND BACTERIA IN AIR CONDITIONING DUCTS. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES009700559A ES2126513B1 (en) | 1997-03-14 | 1997-03-14 | PROCEDURE FOR THE DETECTION OF FUNGI AND BACTERIA IN AIR CONDITIONING DUCTS. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2126513A1 true ES2126513A1 (en) | 1999-03-16 |
ES2126513B1 ES2126513B1 (en) | 1999-12-01 |
Family
ID=8298630
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES009700559A Expired - Fee Related ES2126513B1 (en) | 1997-03-14 | 1997-03-14 | PROCEDURE FOR THE DETECTION OF FUNGI AND BACTERIA IN AIR CONDITIONING DUCTS. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2126513B1 (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0428197A2 (en) * | 1989-10-18 | 1991-05-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods of extracting nucleic acids and PCR amplification without using a proteolytic enzyme |
WO1995013368A1 (en) * | 1993-11-11 | 1995-05-18 | Medinnova Sf | Isolation of nucleic acid |
-
1997
- 1997-03-14 ES ES009700559A patent/ES2126513B1/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0428197A2 (en) * | 1989-10-18 | 1991-05-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods of extracting nucleic acids and PCR amplification without using a proteolytic enzyme |
US5543305A (en) * | 1989-10-18 | 1996-08-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods of extracting deoxyribonucleic acids without using a proteolytic enzyme |
WO1995013368A1 (en) * | 1993-11-11 | 1995-05-18 | Medinnova Sf | Isolation of nucleic acid |
Non-Patent Citations (4)
Title |
---|
AKANE, A. et al. "Identification of the Heme Compound Copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification", JOURNAL OF FORENSIC SCIENCES, 1994, Vol. 39, No 2, paginas 362-372 * |
BASE DE DATOS WPI, semana 9515, Londres: Derwent Publications Ltd., AN 95-063357, Clase B04, C07, D16, JP 06-339399 A (YAMAGUCHI H) 01.06.1993, resumen. * |
FORBES, B.A. et al. "Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: Effects of Bovine Serum Albumin", JOURNAL OF CLINICAL MICROBIOLOGY, 1996, Vol. 34, No 9, paginas 2125-2128 * |
McGREGOR, D.P. et al. "Simultaneous detection of microorganism in soil suspension based on PCR amplification of bacterial 16S rRNA fragments", BIOTECHNIQUES, 1996, Vol. 21, No 3, paginas 463-471 * |
Also Published As
Publication number | Publication date |
---|---|
ES2126513B1 (en) | 1999-12-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20180807 |