AU2001217380A1 - Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample - Google Patents

Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample

Info

Publication number
AU2001217380A1
AU2001217380A1 AU2001217380A AU1738001A AU2001217380A1 AU 2001217380 A1 AU2001217380 A1 AU 2001217380A1 AU 2001217380 A AU2001217380 A AU 2001217380A AU 1738001 A AU1738001 A AU 1738001A AU 2001217380 A1 AU2001217380 A1 AU 2001217380A1
Authority
AU
Australia
Prior art keywords
nucleic acid
sample
mutaiton
detecting
acid fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001217380A
Inventor
Gerrit Johannis De Vos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ingeny Holding BV
Original Assignee
Ingeny Holding BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeny Holding BV filed Critical Ingeny Holding BV
Publication of AU2001217380A1 publication Critical patent/AU2001217380A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients

Abstract

A method and apparatus for the detection of polymorphisms in a nucleic acid sample (e.g. blood, sperm, saliva, cells, . . . ). To enhance the efficiency and the reliability of the known methods (e.g. DGGE, SSCP and TGGE) the amplification process (e.g. PCR) preceding the actual detection step is performed in or on the polyacrylamide gel. Multiple gradients (of chemical denaturants, thermal denaturants and of porosity of the gel matrix) are used for the separation of DNA fragments, by zone electrophoresis on gel slabs or by capillary electrophoresis.
AU2001217380A 2000-10-23 2000-10-23 Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample Abandoned AU2001217380A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/NL2000/000769 WO2002038809A1 (en) 2000-10-23 2000-10-23 Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample

Publications (1)

Publication Number Publication Date
AU2001217380A1 true AU2001217380A1 (en) 2002-05-21

Family

ID=19760714

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001217380A Abandoned AU2001217380A1 (en) 2000-10-23 2000-10-23 Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample

Country Status (9)

Country Link
US (1) US7659056B1 (en)
EP (1) EP1330541B1 (en)
JP (1) JP2004512850A (en)
CN (1) CN100357450C (en)
AT (1) ATE403748T1 (en)
AU (1) AU2001217380A1 (en)
CA (1) CA2426509A1 (en)
DE (1) DE60039792D1 (en)
WO (1) WO2002038809A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2842827B1 (en) * 2002-07-26 2004-10-22 Biocortech NOVEL METHOD OF NUCLEIC ACID ANALYSIS AND ITS USE FOR EVALUATING THE DEGREE OF EDITING OF THE 5-HT2c RECEPTOR mRNA
ES2315196B1 (en) * 2007-09-11 2010-01-12 Instituto Bernabeu, S.L. STUDY OF GENETIC INFORMATIVITY.
GB0818609D0 (en) 2008-10-10 2008-11-19 Univ Hull apparatus and method
DE112010002222B4 (en) * 2009-06-04 2024-01-25 Leidos Innovations Technology, Inc. (n.d.Ges.d. Staates Delaware) Multi-sample microfluidic chip for DNA analysis
CN101619355B (en) * 2009-07-02 2012-02-08 黑龙江大学 Detection method of germs in traditional zymotic soybean paste
US8961764B2 (en) 2010-10-15 2015-02-24 Lockheed Martin Corporation Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5795720A (en) * 1989-08-19 1998-08-18 Qiagen Gmbh Process and device for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis
JP3124969B2 (en) * 1989-08-19 2001-01-15 キアゲン・ゲーエムベーハー Method and apparatus for separating and detecting mixture components by temperature gradient gel electrophoresis
DE4234086A1 (en) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio METHOD FOR DETERMINING NUCLEIC ACID SEQUENCES AMPLIFIED IN VITRO
RU2048522C1 (en) * 1992-10-14 1995-11-20 Институт белка РАН Method of nucleic acid copying, method of their expression and a medium for their realization
IT1282607B1 (en) * 1996-02-13 1998-03-31 Pier Giorgio Righetti USE OF MULTIPLE GRADIENTS FOR THE ANALYSIS OF MUTATIONS IN HUMAN GENOMIC DNA
US5795748A (en) * 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
US6007990A (en) * 1997-04-29 1999-12-28 Levine; Robert A. Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6261431B1 (en) * 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
WO2001016372A1 (en) * 1999-08-27 2001-03-08 Matrix Technologies Corporation Methods of immobilizing ligands on solid supports and apparatus and methods of use therefor
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides

Also Published As

Publication number Publication date
JP2004512850A (en) 2004-04-30
EP1330541B1 (en) 2008-08-06
US7659056B1 (en) 2010-02-09
EP1330541A1 (en) 2003-07-30
ATE403748T1 (en) 2008-08-15
WO2002038809A1 (en) 2002-05-16
CN100357450C (en) 2007-12-26
CN1461348A (en) 2003-12-10
CA2426509A1 (en) 2002-05-16
DE60039792D1 (en) 2008-09-18

Similar Documents

Publication Publication Date Title
França et al. A review of DNA sequencing techniques
Karger et al. DNA sequencing by CE
Slatko et al. “First generation” automated DNA sequencing technology
US5415758A (en) Method and apparatus for electro-elution of biological molecules
CA2696971A1 (en) Micro-channel chip for electrophoresis and method for electrophoresis
Huge et al. Capillary electrophoresis coupled with automated fraction collection
AU2001217380A1 (en) Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample
NO20011607L (en) Method for analyzing polynucleotides
Mitchelson et al. Capillary electrophoresis of nucleic acids
EP0843019A3 (en) Method for determination of specific nucleic acid sequence, and a reagent therefor
Chen et al. High-throughput DNA analysis by microchip electrophoresis
Hadd et al. Sub-microliter DNA sequencing for capillary array electrophoresis
EP1423499B1 (en) Concentration and cleanup of nucleic acid samples
DE60332468D1 (en) analyte
CN111575386B (en) Fluorescent composite amplification kit for detecting human Y-SNP locus and application thereof
IT1282607B1 (en) USE OF MULTIPLE GRADIENTS FOR THE ANALYSIS OF MUTATIONS IN HUMAN GENOMIC DNA
CN109839417B (en) Human autosomal STR (short tandem repeat) gene locus typing method based on micro-fluidic chip
Suenaga et al. Prestaining Method as a Useful Tool for the Agarose Gel Electrophoretic Detection of Polymerase Chain Reaction Products with a Fluorescent Dye SYBR® Gold Nucleic Acid Gel Stain
Li et al. On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels
WO1999058971A3 (en) Methods for quantitating low level modifications of nucleotide sequences
DE60321449D1 (en) METHOD FOR THE TWO-DIMENSIONAL CONFORMATION-DEPENDENT SEPARATION OF NONCIRCULAR NUCLEIC ACIDS
Liu et al. Automated DNA mutation analysis by single-strand conformation polymorphism using capillary electrophoresis with laser-induced fluorescence detection
EP1004676A4 (en) Methods for dna amplification and kits therefor
CN108118088B (en) Human Y-STR gene locus typing method based on micro-fluidic chip
Liu et al. Rapid analysis of amplified double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection