ES2120888A1 - Quantitative immunoenzymatic method for detecting the adulteration of ewe's milk with milk of another species. - Google Patents

Quantitative immunoenzymatic method for detecting the adulteration of ewe's milk with milk of another species.

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Publication number
ES2120888A1
ES2120888A1 ES09601384A ES9601384A ES2120888A1 ES 2120888 A1 ES2120888 A1 ES 2120888A1 ES 09601384 A ES09601384 A ES 09601384A ES 9601384 A ES9601384 A ES 9601384A ES 2120888 A1 ES2120888 A1 ES 2120888A1
Authority
ES
Spain
Prior art keywords
milk
ewe
washing
well
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09601384A
Other languages
Spanish (es)
Other versions
ES2120888B1 (en
Inventor
Rebollar Miguel Calvo
Cabrejas M Dolores Perez
Casquero Pedro Razquin
Aramendia Luis Angel Inda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Zaragoza
Original Assignee
Universidad de Zaragoza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Zaragoza filed Critical Universidad de Zaragoza
Priority to ES9601384A priority Critical patent/ES2120888B1/en
Publication of ES2120888A1 publication Critical patent/ES2120888A1/en
Application granted granted Critical
Publication of ES2120888B1 publication Critical patent/ES2120888B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Quantitative immunoenzymatic method for detecting the presence of milk of a different species in ewe's milk, which involves: a) depositing a specific antibody to milk protein on the wells of a plate of polystyrene or other plastic material capable of fixing proteins b) washing to eliminate the antibodies not fixed to the plate c) incubating with a solution of ovalbumin and then washing d) applying to each well of the plate the sample of the milk to be analysed, or the standard e) washing to eliminate the proteins which have not reacted with the antibodies f) incubating each well of the plate with anti-milk-protein immunoglobulins, directed against the same protein as used in stage "a", linked to peroxidase or alkaline phosphatase, and diluted in physiological serum at ambient temperature g) washing to eliminate the labelled antibodies which have not reacted h) incubating each well with a substrate of the enzyme to develop it i) measuring the absorbance of each well. It has applications because it allows determination of the presence and quantification of milk of a different species in ewe's milk and ewe's milk cheese.
ES9601384A 1996-06-06 1996-06-06 QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES. Expired - Fee Related ES2120888B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9601384A ES2120888B1 (en) 1996-06-06 1996-06-06 QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9601384A ES2120888B1 (en) 1996-06-06 1996-06-06 QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES.

Publications (2)

Publication Number Publication Date
ES2120888A1 true ES2120888A1 (en) 1998-11-01
ES2120888B1 ES2120888B1 (en) 1999-05-16

Family

ID=8295236

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9601384A Expired - Fee Related ES2120888B1 (en) 1996-06-06 1996-06-06 QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES.

Country Status (1)

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ES (1) ES2120888B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2195772A1 (en) * 2002-02-21 2003-12-01 Univ De Zaragoza Y En Su Nombr Quantitative immuno enzymatic detection of e.g. milk adulteration, consists of deposition of vegetable proteins for incubation and absorbency measurement
CN109374879A (en) * 2018-09-18 2019-02-22 北京纳百生物科技有限公司 Mixed with the detection kit and its detection method of milk elements in a kind of sheep cream and sheep milk powder

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR1009998B (en) * 2011-07-21 2021-05-12 Αντωνιος Νικολαου Νταντασιος A sandwich immunoessay device using sheep anti-goat igg to immunologically detect goat's milk in sheep's milk

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2005218A6 (en) * 1987-05-11 1989-03-01 Univ Zaragoza Detection of milk from other species in sheep's milk or cheese
ES2017031A6 (en) * 1989-07-28 1990-12-16 Univ Zaragoza Rapid immunoenzymatic method for detecting the presence of milk of a different type in ewe's milk

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2005218A6 (en) * 1987-05-11 1989-03-01 Univ Zaragoza Detection of milk from other species in sheep's milk or cheese
ES2017031A6 (en) * 1989-07-28 1990-12-16 Univ Zaragoza Rapid immunoenzymatic method for detecting the presence of milk of a different type in ewe's milk

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FOOD CONTROL, Vol. 4, N‘ 2, 1993, P. ARANDA et al. "Rapid inmunoenzymatic method for detecting adulteration in ewe's milk", pßginas 101-104 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2195772A1 (en) * 2002-02-21 2003-12-01 Univ De Zaragoza Y En Su Nombr Quantitative immuno enzymatic detection of e.g. milk adulteration, consists of deposition of vegetable proteins for incubation and absorbency measurement
CN109374879A (en) * 2018-09-18 2019-02-22 北京纳百生物科技有限公司 Mixed with the detection kit and its detection method of milk elements in a kind of sheep cream and sheep milk powder
CN109374879B (en) * 2018-09-18 2021-07-06 北京纳百生物科技有限公司 Detection kit for cow milk component doped in goat milk and goat milk powder and detection method thereof

Also Published As

Publication number Publication date
ES2120888B1 (en) 1999-05-16

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