ES2120888A1 - Quantitative immunoenzymatic method for detecting the adulteration of ewe's milk with milk of another species. - Google Patents
Quantitative immunoenzymatic method for detecting the adulteration of ewe's milk with milk of another species.Info
- Publication number
- ES2120888A1 ES2120888A1 ES09601384A ES9601384A ES2120888A1 ES 2120888 A1 ES2120888 A1 ES 2120888A1 ES 09601384 A ES09601384 A ES 09601384A ES 9601384 A ES9601384 A ES 9601384A ES 2120888 A1 ES2120888 A1 ES 2120888A1
- Authority
- ES
- Spain
- Prior art keywords
- milk
- ewe
- washing
- well
- plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Quantitative immunoenzymatic method for detecting the presence of milk of a different species in ewe's milk, which involves: a) depositing a specific antibody to milk protein on the wells of a plate of polystyrene or other plastic material capable of fixing proteins b) washing to eliminate the antibodies not fixed to the plate c) incubating with a solution of ovalbumin and then washing d) applying to each well of the plate the sample of the milk to be analysed, or the standard e) washing to eliminate the proteins which have not reacted with the antibodies f) incubating each well of the plate with anti-milk-protein immunoglobulins, directed against the same protein as used in stage "a", linked to peroxidase or alkaline phosphatase, and diluted in physiological serum at ambient temperature g) washing to eliminate the labelled antibodies which have not reacted h) incubating each well with a substrate of the enzyme to develop it i) measuring the absorbance of each well. It has applications because it allows determination of the presence and quantification of milk of a different species in ewe's milk and ewe's milk cheese.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601384A ES2120888B1 (en) | 1996-06-06 | 1996-06-06 | QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601384A ES2120888B1 (en) | 1996-06-06 | 1996-06-06 | QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2120888A1 true ES2120888A1 (en) | 1998-11-01 |
ES2120888B1 ES2120888B1 (en) | 1999-05-16 |
Family
ID=8295236
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9601384A Expired - Fee Related ES2120888B1 (en) | 1996-06-06 | 1996-06-06 | QUANTITATIVE IMMUNOENZYMATIC METHOD TO DETECT THE ADULTERATION OF SHEEP'S MILK WITH MILK OF ANOTHER SPECIES. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2120888B1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2195772A1 (en) * | 2002-02-21 | 2003-12-01 | Univ De Zaragoza Y En Su Nombr | Quantitative immuno enzymatic detection of e.g. milk adulteration, consists of deposition of vegetable proteins for incubation and absorbency measurement |
CN109374879A (en) * | 2018-09-18 | 2019-02-22 | 北京纳百生物科技有限公司 | Mixed with the detection kit and its detection method of milk elements in a kind of sheep cream and sheep milk powder |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GR1009998B (en) * | 2011-07-21 | 2021-05-12 | Αντωνιος Νικολαου Νταντασιος | A sandwich immunoessay device using sheep anti-goat igg to immunologically detect goat's milk in sheep's milk |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2005218A6 (en) * | 1987-05-11 | 1989-03-01 | Univ Zaragoza | Detection of milk from other species in sheep's milk or cheese |
ES2017031A6 (en) * | 1989-07-28 | 1990-12-16 | Univ Zaragoza | Rapid immunoenzymatic method for detecting the presence of milk of a different type in ewe's milk |
-
1996
- 1996-06-06 ES ES9601384A patent/ES2120888B1/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2005218A6 (en) * | 1987-05-11 | 1989-03-01 | Univ Zaragoza | Detection of milk from other species in sheep's milk or cheese |
ES2017031A6 (en) * | 1989-07-28 | 1990-12-16 | Univ Zaragoza | Rapid immunoenzymatic method for detecting the presence of milk of a different type in ewe's milk |
Non-Patent Citations (1)
Title |
---|
FOOD CONTROL, Vol. 4, N‘ 2, 1993, P. ARANDA et al. "Rapid inmunoenzymatic method for detecting adulteration in ewe's milk", pßginas 101-104 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2195772A1 (en) * | 2002-02-21 | 2003-12-01 | Univ De Zaragoza Y En Su Nombr | Quantitative immuno enzymatic detection of e.g. milk adulteration, consists of deposition of vegetable proteins for incubation and absorbency measurement |
CN109374879A (en) * | 2018-09-18 | 2019-02-22 | 北京纳百生物科技有限公司 | Mixed with the detection kit and its detection method of milk elements in a kind of sheep cream and sheep milk powder |
CN109374879B (en) * | 2018-09-18 | 2021-07-06 | 北京纳百生物科技有限公司 | Detection kit for cow milk component doped in goat milk and goat milk powder and detection method thereof |
Also Published As
Publication number | Publication date |
---|---|
ES2120888B1 (en) | 1999-05-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19981101 Kind code of ref document: A1 Effective date: 19981101 |
|
FD2A | Announcement of lapse in spain |
Effective date: 20160926 |