ES2112215A1 - Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260". - Google Patents

Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260".

Info

Publication number
ES2112215A1
ES2112215A1 ES09601666A ES9601666A ES2112215A1 ES 2112215 A1 ES2112215 A1 ES 2112215A1 ES 09601666 A ES09601666 A ES 09601666A ES 9601666 A ES9601666 A ES 9601666A ES 2112215 A1 ES2112215 A1 ES 2112215A1
Authority
ES
Spain
Prior art keywords
compounds
synthesis
aromatic compounds
biodegradation
pigments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09601666A
Other languages
English (en)
Other versions
ES2112215B1 (es
Inventor
Fernandez Margarita Martin
Munoz Estrella Ferrer
Alvarez Jesus Blanco
Prieto Alicia Gibello
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Complutense de Madrid
Original Assignee
Universidad Complutense de Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Complutense de Madrid filed Critical Universidad Complutense de Madrid
Priority to ES09601666A priority Critical patent/ES2112215B1/es
Priority to AU36239/97A priority patent/AU3623997A/en
Priority to EP97932844A priority patent/EP0857779A1/en
Priority to PCT/ES1997/000189 priority patent/WO1998004679A1/es
Publication of ES2112215A1 publication Critical patent/ES2112215A1/es
Application granted granted Critical
Publication of ES2112215B1 publication Critical patent/ES2112215B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hydrology & Water Resources (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EL PROCEDIMIENTO MICROBIOLOGICO PARA LA BIODEGRADACION DE COMPUESTOS AROMATICOS Y SINTESIS DE PIGMENTOS Y COLORANTES, ALCALOIDES Y POLIMEROS, MEDIANTE LA UTILIZACION LA CEPA RECOMBINANTE E.COLI P-260, SE CARACTERIZA POR LA EXPRESION CONSTITUTIVA DE LA ENZIMA 4-HPA HIDROXILASA DE K.PNEUMONIAE. ESTA ENZIMA PARTICIPA EN LA DEGRADACION DEL ACIDO 4-HIDROXIFENILACETICO (4-HPA), UNO DE LOS COMPUESTOS PRESENTES EN EL "ALPECHIN". LA BAJA ESPECIFICIDAD DE SUSTRATO DE LA ENZIMA Y SU DOBLE ACTIVIDAD CATALITICA: A) HIDROXILACION DE COMPUESTOS MONO-HIDROXILADOS A INTEMMEDIARIOS DI-HIDROXILADOS Y B) OXIDACION DE COMPUESTOS DI-HIDROXILADOS A QUINONAS, PERMITE SU UTILIZACION EN UNA DOBLE VERTIENTE: 1) DEGRADACION DE COMPUESTOS AROMATICOS TOXICOS DEL MEDIO AMBIENTE Y 2) SINTESIS DE COMPUESTOS ORGANICOS DE INTERES INDUSTRIAL, CUYA BASE ESTRUCTURAL SEA UNA QUINONA.
ES09601666A 1996-07-26 1996-07-26 Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260". Expired - Fee Related ES2112215B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES09601666A ES2112215B1 (es) 1996-07-26 1996-07-26 Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260".
AU36239/97A AU3623997A (en) 1996-07-26 1997-07-24 Process for the biodegradation of aromatic compounds and synthesis of pigments and colorants, alcaloids and polymers, with the use of the recombinant strain escherichia coli p260
EP97932844A EP0857779A1 (en) 1996-07-26 1997-07-24 Process for the biodegradation of aromatic compounds and synthesis of pigments and colorants, alcaloids and polymers, with the use of the recombinant strain escherichia coli p260
PCT/ES1997/000189 WO1998004679A1 (es) 1996-07-26 1997-07-24 Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes, alcaloides y polimeros utilizando la cepa recombinante escherichia coli p260

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09601666A ES2112215B1 (es) 1996-07-26 1996-07-26 Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260".

Publications (2)

Publication Number Publication Date
ES2112215A1 true ES2112215A1 (es) 1998-03-16
ES2112215B1 ES2112215B1 (es) 1998-10-01

Family

ID=8295634

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09601666A Expired - Fee Related ES2112215B1 (es) 1996-07-26 1996-07-26 Procedimiento para la biodegradacion de compuestos aromaticos y sintesis de pigmentos y colorantes alcaloides y polimeros utilizando la cepa recombinante "escherichia coli p260".

Country Status (4)

Country Link
EP (1) EP0857779A1 (es)
AU (1) AU3623997A (es)
ES (1) ES2112215B1 (es)
WO (1) WO1998004679A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007026176A2 (en) * 2005-09-02 2007-03-08 Ringfort Patents Limited Condiment

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0718397A2 (en) * 1994-09-13 1996-06-26 Tratamiento Integral De Alpechines Baena, S.L. Procedure for the purification and development of liquid and solid waste product produced by oil mill

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0718397A2 (en) * 1994-09-13 1996-06-26 Tratamiento Integral De Alpechines Baena, S.L. Procedure for the purification and development of liquid and solid waste product produced by oil mill

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ARUNACHALAM, U. et al. "P-Hydroxyphenylacetate-3-hydroxylase". THE JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, Vol. 267, N‘ 36, pßginas 25848-25855 * Todo el documento * *
GIBELLO, A. et al. "Polymer production by Klebsiella pneumoniae 4-Hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli". APPLIED AND ENVIROMENTAL MICROBIOLOGY, 1995, Vol. 61, N‘ 12, pßginas 4167-4171 * Todo el documento * *
MARTIN, M. et al. "Catabolism of 3-and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae". JOURNAL OF GENERAL MICROBIOLOGY, 1991, Vol. 132, pßginas 621-628 * Todo el documento * *
PRIETO, M.A. et al. "Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range". JOURNAL OF BACTERIOLOGY, 1993, Vol. 175, N‘ 7, pßginas 2162-2167 * Todo el documento * *

Also Published As

Publication number Publication date
AU3623997A (en) 1998-02-20
EP0857779A1 (en) 1998-08-12
WO1998004679A1 (es) 1998-02-05
ES2112215B1 (es) 1998-10-01

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