ES2111495A1 - Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia. - Google Patents

Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia.

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Publication number
ES2111495A1
ES2111495A1 ES09601614A ES9601614A ES2111495A1 ES 2111495 A1 ES2111495 A1 ES 2111495A1 ES 09601614 A ES09601614 A ES 09601614A ES 9601614 A ES9601614 A ES 9601614A ES 2111495 A1 ES2111495 A1 ES 2111495A1
Authority
ES
Spain
Prior art keywords
baking
strain
production
yeast
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09601614A
Other languages
English (en)
Other versions
ES2111495B1 (es
Inventor
Bigorra Pascual Sanz
Alaman Jose Antonio Priego
Gonzalez Jose Antonio Perez
Vidal Daniel Ramon
Roig Aurelia Monfort
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9601614A priority Critical patent/ES2111495B1/es
Priority to AU35440/97A priority patent/AU3544097A/en
Priority to PCT/ES1997/000177 priority patent/WO1998003630A1/es
Publication of ES2111495A1 publication Critical patent/ES2111495A1/es
Application granted granted Critical
Publication of ES2111495B1 publication Critical patent/ES2111495B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/047—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

CEPA DE LEVADURA DE PANADERIA CECT10868 Y CEPA DE PANADERIA CECT10869. SU METODO DE OBTENCION POR TECNICAS DE ADN RECOMBINANTE Y SU APLICACION COMO LEVADURAS DE PANADERIA. LA PRESENTE INVENCION CONSISTE EN LA OBTENCION DE UNA CEPA INDUSTRIAL DE LEVADURA DE PANADERIA CAPAZ DE EXPRESAR Y, EXPORTAR AL EXTERIOR CELULAR LA ENZIMA ENDOXILANASA X24 DE ASPERGILLUS NIDULANS Y EN LA OBTENCION DE UNA CEPA INDUSTRIAL DE PANADERIA CAPAZ DE EXPRESAR Y EXPORTAR AL EXTERIOR CELULAR. CONJUNTAMENTE, LA ENZIMA ENDOXILANASA X24 DE ASPERGILLUS NIDULANS Y LA ENZIMA AL-AMILASA DE ASPERGILLUS ORYZAE, CON EL FIN DE AUMENTAR EL PROCESO DE DEGRADACION DE LAS HEMICELULOSAS Y, ALMIDON PRESENTES EN LA HARINA Y, CONSECUENTEMENTE, MEJORAR EL VOLUMEN, TEXTURA Y, VIDA MEDIA DEL PAN ELABORADO. PARA ELLO SE HAN INTRODUCIDO EN LA LEVADURA LOS GENES CORRESPONDIENTES A ESTAS ENZIMAS Y SU EXPRESION SE HA REGULADO POR UN PROMOTOR TIPICO DE LEVADURA. COMO ES EL DEL GEN DE LA ACTINA (PACT1), QUE ES, ADEMAS, FUNCIONAL EN LAS CONDICIONES INDUSTRIALES DE CRECIMIENTO EN MELAZAS. SU APLICACION EN TECNICAS DE INGENIERIA GENETICA Y LEVADURA DE PANADERIA.
ES9601614A 1996-07-19 1996-07-19 Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia. Expired - Fee Related ES2111495B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9601614A ES2111495B1 (es) 1996-07-19 1996-07-19 Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia.
AU35440/97A AU3544097A (en) 1996-07-19 1997-07-15 Baking yeast strain cect10868 and baking strain cect10869; method for the production thereof by means of recombinant dna techniques and their application as baking yeasts
PCT/ES1997/000177 WO1998003630A1 (es) 1996-07-19 1997-07-15 Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869, su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9601614A ES2111495B1 (es) 1996-07-19 1996-07-19 Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia.

Publications (2)

Publication Number Publication Date
ES2111495A1 true ES2111495A1 (es) 1998-03-01
ES2111495B1 ES2111495B1 (es) 1998-11-01

Family

ID=8295553

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9601614A Expired - Fee Related ES2111495B1 (es) 1996-07-19 1996-07-19 Cepa de levadura de panaderia cect10868 y cepa de panaderia cect10869. su metodo de obtencion por tecnicas de adn recombinante y su aplicacion como levaduras de panaderia.

Country Status (3)

Country Link
AU (1) AU3544097A (es)
ES (1) ES2111495B1 (es)
WO (1) WO1998003630A1 (es)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019782A1 (en) * 1990-06-19 1991-12-26 Unilever N.V. Xylanase production
EP0463706A1 (en) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Cloning and expression of xylanase genes from fungal origin
WO1994014965A1 (en) * 1992-12-24 1994-07-07 Gist-Brocades N.V. Cloning and expression of xylanase b
WO1994021785A1 (en) * 1993-03-10 1994-09-29 Novo Nordisk A/S Enzymes with xylanase activity from aspergillus aculeatus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019782A1 (en) * 1990-06-19 1991-12-26 Unilever N.V. Xylanase production
EP0463706A1 (en) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Cloning and expression of xylanase genes from fungal origin
WO1994014965A1 (en) * 1992-12-24 1994-07-07 Gist-Brocades N.V. Cloning and expression of xylanase b
WO1994021785A1 (en) * 1993-03-10 1994-09-29 Novo Nordisk A/S Enzymes with xylanase activity from aspergillus aculeatus

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FERNANDEZ-ESPINAR, M.T. et al. "Construction of an Aspergillus nidulans multicopy transformant for the xlnB gene and its use in purifying the minor X24 xylanase", APPLIED AND ENVIROMENTAL MICROBIOLOGY, 1996, Vol. 45, pßginas 338-341 * Todo el documento * *
FERNANDEZ-ESPINAR, M.T. et al. "Xylanase production by Aspergillus nidulans", FEMS MICROBIOLOGY LETTERS, 1992, Vol. 91, pßginas 91-96 * Todo el documento * *
PEREZ-GONZALEZ, J.A. et al. "Molecular cloning and expression in Saccharomyces cerevisae of two Aspergillus nidulans xylanase genes", APPLIED AND ENVIROMENTAL MICROBIOLOGY, 1996, Vol. 62, N‘ 6, pßginas 2179-2182 * Todo el documento * *
PIÐAGA, F. et al. "Xylanase production in Aspergillus nidulans: Induction and carbon catabolite repression", FEMS MICROBIOLOGY LETTERS, 1994, Vol. 115, pßginas 319-324 * Todo el documento * *
RANDEZ-GIL, F. et al. "Construction of baker's yeast strains that secrete Aspergillus oryzae alpha-anylase and their use in bread making", JOURNAL OF CEREAL SCIENCE, 1995, Vol. 21, pßginas 185-193 * Todo el documento * *

Also Published As

Publication number Publication date
WO1998003630A1 (es) 1998-01-29
AU3544097A (en) 1998-02-10
ES2111495B1 (es) 1998-11-01

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