ES2088823A1 - Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. - Google Patents
Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones.Info
- Publication number
- ES2088823A1 ES2088823A1 ES09401094A ES9401094A ES2088823A1 ES 2088823 A1 ES2088823 A1 ES 2088823A1 ES 09401094 A ES09401094 A ES 09401094A ES 9401094 A ES9401094 A ES 9401094A ES 2088823 A1 ES2088823 A1 ES 2088823A1
- Authority
- ES
- Spain
- Prior art keywords
- identification
- determination
- products obtained
- oligonucleotides
- taxonomic groups
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA LA OBTENCION DE OLIGONUCLEOTIDOS Y SONDAS DESTINADOS A LA DETERMINACION E IDENTIFICACION DE GRUPOS TAXONOMICOS, PRODUCTOS OBTENIDOS Y APLICACIONES. EL METODO COMPRENDE: (A) SELECCIONAR LAS CEPAS; (B) EXTRAER EL DNA; (C) SELECCIONAR OLIGONUCLEOTIDOS CAPACES DE HIBRIDAR CON UNA DE LAS HEBRAS DEL DNA Y QUE POSEAN UN EXTREMO 3''-HIDROXILO LIBRE, COMO CEBADORES; (D) EFECTUAR LA REACCION EN CADENA DE LA POLIMERASA; (E) ANALIZAR LOS FRAGMENTOS DE DNA QUE HAN SIDO AMPLIFICADOS; (F) SELECCIONAR LAS BANDAS COMUNES A TODOS LOS ORGANISMOS DEL TAXON, ENTRE LOS PATRONES DE AP-PCR; (G) SECUENCIAR LOS EXTREMOS DE LOS FRAGMENTOS DE AP-PCR COMUNES; (H) DISEÑAR OLIGONUCLEOTIDOS ESPECIFICOS COMPLEMENTARIOS DE LAS SECUENCIAS OBTENIDAS; (I) DETECTAR LA PRESENCIA DE FRAGMENTOS ESPECIFICOS EN EL GENOMA DE UN INDIVIDUO. LOS PRODUCTOS OBTENIDOS POR ESTE METODO TIENEN APLICACION PARA LA DETERMINACION E IDENTIFICACION DE GRUPOS TAXONOMICOS, POR EJEMPLO, BACTERIAS, ENTRE LAS QUE DESTACA LA ESPECIE CVAHALOFERAX MEDITERRANEI.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09401094A ES2088823B1 (es) | 1994-05-18 | 1994-05-18 | Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09401094A ES2088823B1 (es) | 1994-05-18 | 1994-05-18 | Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2088823A1 true ES2088823A1 (es) | 1996-09-16 |
ES2088823B1 ES2088823B1 (es) | 1997-05-01 |
Family
ID=8286309
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09401094A Expired - Fee Related ES2088823B1 (es) | 1994-05-18 | 1994-05-18 | Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2088823B1 (es) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0395292A2 (en) * | 1989-04-20 | 1990-10-31 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
-
1994
- 1994-05-18 ES ES09401094A patent/ES2088823B1/es not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0395292A2 (en) * | 1989-04-20 | 1990-10-31 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
Non-Patent Citations (6)
Title |
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CANCILLA, M.R., et al., Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with p32 and fluorescent labels, Appl. Environ. Microbiol., Mayo 1992, Vol.58, No.5, pßgs. 1772-1775. * Ver el documento completo * * |
CROWHURST, R.N., et al., Differentiation of Fusarium solari f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA, Curr. Genet., 1991, Vol.20, pßgs. 391-396. * Ver el documento completo * * |
FANI, R. et al., A general method for generating specific DNA probes by arbitrarily-primed PCR (AP-PCR), ECB6: Proceedings of the 6th Eupopean Congress on Biotechnology, part 1, 1993, Amsterdam: Elsevier (Alberghina, L. et al.), 1994, pßgs. 363-366. * Ver el documento completo * * |
HAMELIN, R.C., et al., Identification of Gremmeniella abietina races with random apmlified polymorphic DNA markers, Appl. Environ. Microbiol., Junio 1993, Vol.59, No.6, pßgs. 1752-1755. * Ver el documento completo * * |
WELSH, J. et al., Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Research, Diciembre 1990, Vol.18, No.24, pßgs. 7213-7218. * Ver el documento completo * * |
WILLIAMS, J.G., et al., DNA polymorphisms amplified by arbritary primers are useful as genetic markers, Nucleic Acids Research, Noviembre 1990, Vol.18, No.22, pßgs. 6531-6535. * Ver el documento completo * * |
Also Published As
Publication number | Publication date |
---|---|
ES2088823B1 (es) | 1997-05-01 |
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