ES2088823A1 - Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. - Google Patents

Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones.

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Publication number
ES2088823A1
ES2088823A1 ES09401094A ES9401094A ES2088823A1 ES 2088823 A1 ES2088823 A1 ES 2088823A1 ES 09401094 A ES09401094 A ES 09401094A ES 9401094 A ES9401094 A ES 9401094A ES 2088823 A1 ES2088823 A1 ES 2088823A1
Authority
ES
Spain
Prior art keywords
identification
determination
products obtained
oligonucleotides
taxonomic groups
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09401094A
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English (en)
Other versions
ES2088823B1 (es
Inventor
Murcia Antonio Martinez
Valera Francisco Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Alicante
Original Assignee
Universidad de Alicante
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Alicante filed Critical Universidad de Alicante
Priority to ES09401094A priority Critical patent/ES2088823B1/es
Publication of ES2088823A1 publication Critical patent/ES2088823A1/es
Application granted granted Critical
Publication of ES2088823B1 publication Critical patent/ES2088823B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

METODO PARA LA OBTENCION DE OLIGONUCLEOTIDOS Y SONDAS DESTINADOS A LA DETERMINACION E IDENTIFICACION DE GRUPOS TAXONOMICOS, PRODUCTOS OBTENIDOS Y APLICACIONES. EL METODO COMPRENDE: (A) SELECCIONAR LAS CEPAS; (B) EXTRAER EL DNA; (C) SELECCIONAR OLIGONUCLEOTIDOS CAPACES DE HIBRIDAR CON UNA DE LAS HEBRAS DEL DNA Y QUE POSEAN UN EXTREMO 3''-HIDROXILO LIBRE, COMO CEBADORES; (D) EFECTUAR LA REACCION EN CADENA DE LA POLIMERASA; (E) ANALIZAR LOS FRAGMENTOS DE DNA QUE HAN SIDO AMPLIFICADOS; (F) SELECCIONAR LAS BANDAS COMUNES A TODOS LOS ORGANISMOS DEL TAXON, ENTRE LOS PATRONES DE AP-PCR; (G) SECUENCIAR LOS EXTREMOS DE LOS FRAGMENTOS DE AP-PCR COMUNES; (H) DISEÑAR OLIGONUCLEOTIDOS ESPECIFICOS COMPLEMENTARIOS DE LAS SECUENCIAS OBTENIDAS; (I) DETECTAR LA PRESENCIA DE FRAGMENTOS ESPECIFICOS EN EL GENOMA DE UN INDIVIDUO. LOS PRODUCTOS OBTENIDOS POR ESTE METODO TIENEN APLICACION PARA LA DETERMINACION E IDENTIFICACION DE GRUPOS TAXONOMICOS, POR EJEMPLO, BACTERIAS, ENTRE LAS QUE DESTACA LA ESPECIE CVAHALOFERAX MEDITERRANEI.
ES09401094A 1994-05-18 1994-05-18 Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones. Expired - Fee Related ES2088823B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09401094A ES2088823B1 (es) 1994-05-18 1994-05-18 Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09401094A ES2088823B1 (es) 1994-05-18 1994-05-18 Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones.

Publications (2)

Publication Number Publication Date
ES2088823A1 true ES2088823A1 (es) 1996-09-16
ES2088823B1 ES2088823B1 (es) 1997-05-01

Family

ID=8286309

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09401094A Expired - Fee Related ES2088823B1 (es) 1994-05-18 1994-05-18 Metodo para la obtencion de oligonucleotidos y sondas destinados a la determinacion e identificacion de grupos taxonomicos, productos obtenidos y aplicaciones.

Country Status (1)

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ES (1) ES2088823B1 (es)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0395292A2 (en) * 1989-04-20 1990-10-31 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0395292A2 (en) * 1989-04-20 1990-10-31 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CANCILLA, M.R., et al., Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with p32 and fluorescent labels, Appl. Environ. Microbiol., Mayo 1992, Vol.58, No.5, pßgs. 1772-1775. * Ver el documento completo * *
CROWHURST, R.N., et al., Differentiation of Fusarium solari f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA, Curr. Genet., 1991, Vol.20, pßgs. 391-396. * Ver el documento completo * *
FANI, R. et al., A general method for generating specific DNA probes by arbitrarily-primed PCR (AP-PCR), ECB6: Proceedings of the 6th Eupopean Congress on Biotechnology, part 1, 1993, Amsterdam: Elsevier (Alberghina, L. et al.), 1994, pßgs. 363-366. * Ver el documento completo * *
HAMELIN, R.C., et al., Identification of Gremmeniella abietina races with random apmlified polymorphic DNA markers, Appl. Environ. Microbiol., Junio 1993, Vol.59, No.6, pßgs. 1752-1755. * Ver el documento completo * *
WELSH, J. et al., Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Research, Diciembre 1990, Vol.18, No.24, pßgs. 7213-7218. * Ver el documento completo * *
WILLIAMS, J.G., et al., DNA polymorphisms amplified by arbritary primers are useful as genetic markers, Nucleic Acids Research, Noviembre 1990, Vol.18, No.22, pßgs. 6531-6535. * Ver el documento completo * *

Also Published As

Publication number Publication date
ES2088823B1 (es) 1997-05-01

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