ES2085767T3 - Aumento de la actividad catalitica de las ribozimas por un oligonucleotido facilitador proximo. - Google Patents

Aumento de la actividad catalitica de las ribozimas por un oligonucleotido facilitador proximo.

Info

Publication number
ES2085767T3
ES2085767T3 ES93904711T ES93904711T ES2085767T3 ES 2085767 T3 ES2085767 T3 ES 2085767T3 ES 93904711 T ES93904711 T ES 93904711T ES 93904711 T ES93904711 T ES 93904711T ES 2085767 T3 ES2085767 T3 ES 2085767T3
Authority
ES
Spain
Prior art keywords
catalytic activity
ribozyme
facilitator oligonucleotide
ribozymes
proximate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93904711T
Other languages
English (en)
Inventor
John Goodchild
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Worcester Foundation for Biomedical Research
Original Assignee
Worcester Foundation for Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Foundation for Biomedical Research filed Critical Worcester Foundation for Biomedical Research
Application granted granted Critical
Publication of ES2085767T3 publication Critical patent/ES2085767T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catalysts (AREA)

Abstract

SE DESCRIBEN METODOS PARA INCREMENTAR LA ACTIVIDAD CATALITICA DE LA RIBOZIMA SIN REDUCIR SU ESPECIFICIDAD, QUE COMPRENDEN LA PUESTA EN CONTACTO DE UNA MOLECULA DE ARN CON UNA RIBOZIMA Y UN OLIGONUCLEOTIDO FACILITADOR. EL PRESENTE INVENTO ADEMAS PROPORCIONA COMPOSICIONES QUE CONTIENEN UNA RIBOZIMA Y UNA CANTIDAD EFECTIVA DE OLIGONUCLEOTIDO FACILITADOR. TAMBIEN SE DESCUBREN METODOS PARA REDUCIR LA CONCENTRACION DE MG2+ Y MN2+ REQUERIDA POR UNA RIBOZIMA PARA ADHERIRSE CATALITICAMENTE AL ARN OBJETIVO.
ES93904711T 1992-02-04 1993-02-04 Aumento de la actividad catalitica de las ribozimas por un oligonucleotido facilitador proximo. Expired - Lifetime ES2085767T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83071392A 1992-02-04 1992-02-04
CA002106015A CA2106015A1 (en) 1992-02-04 1993-09-13 Ribozymes having 2'-0 substituted nucleotides in the flanking sequences

Publications (1)

Publication Number Publication Date
ES2085767T3 true ES2085767T3 (es) 1996-06-01

Family

ID=25676634

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93904711T Expired - Lifetime ES2085767T3 (es) 1992-02-04 1993-02-04 Aumento de la actividad catalitica de las ribozimas por un oligonucleotido facilitador proximo.

Country Status (10)

Country Link
EP (1) EP0625194B1 (es)
JP (1) JPH07508638A (es)
AT (1) ATE137263T1 (es)
AU (1) AU661124B2 (es)
CA (2) CA2117428A1 (es)
DE (1) DE69302369T2 (es)
DK (1) DK0625194T3 (es)
ES (1) ES2085767T3 (es)
GR (1) GR3019911T3 (es)
WO (1) WO1993015194A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3933384A1 (de) * 1989-10-06 1991-04-18 Hoechst Ag Multifunktionelle rna mit selbstprozessierungsaktivitaet, ihre herstellung und ihre verwendung
WO1995003406A2 (en) * 1993-07-19 1995-02-02 Gen-Probe Incorporated Enhancement of oligonucleotide inhibition of protein production, cell proliferation, and/or multiplication of infectious disease pathogens
US5739309A (en) * 1993-07-19 1998-04-14 Gen-Probe Incorporated Enhancement of oligonucleotide inhibition of protein production, cell proliferation and / or multiplication of infectious disease pathogens
US5998193A (en) * 1994-06-24 1999-12-07 Gene Shears Pty., Ltd. Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof
US5700923A (en) * 1994-09-29 1997-12-23 Hybridon, Inc. Finderons and methods of their preparation and use
US5650502A (en) * 1994-11-09 1997-07-22 Hybridon, Inc. Ribozyme analogs having rigid non-nucleotidic linkers
US5545729A (en) * 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3933384A1 (de) * 1989-10-06 1991-04-18 Hoechst Ag Multifunktionelle rna mit selbstprozessierungsaktivitaet, ihre herstellung und ihre verwendung

Also Published As

Publication number Publication date
WO1993015194A1 (en) 1993-08-05
AU661124B2 (en) 1995-07-13
DK0625194T3 (da) 1996-05-28
CA2117428A1 (en) 1993-08-05
GR3019911T3 (en) 1996-08-31
AU3597793A (en) 1993-09-01
ATE137263T1 (de) 1996-05-15
EP0625194A1 (en) 1994-11-23
JPH07508638A (ja) 1995-09-28
DE69302369T2 (de) 1996-09-05
CA2106015A1 (en) 1995-03-14
EP0625194B1 (en) 1996-04-24
DE69302369D1 (de) 1996-05-30

Similar Documents

Publication Publication Date Title
ES2065919T3 (es) Ribozimas.
ES2061416T3 (es) Ribozimas modificadas.
DE69628864D1 (de) Invertierte chimäre und hybrid-oligonukleotide
DE69403642T2 (de) 7-deazapurin modifizierte oligonukleotide
DE60030970D1 (de) Verfahren und zusammensetzungen für nichtvirale gentherapie zur behandlung von hyperproliferativen krankheiten
CY1110633T1 (el) Τροποποιηση αντιθετης φορας της εκφρασης σουρβιβινης
FR2715847B1 (fr) Composition contenant des acides nucléiques, préparation et utilisations.
PT1053313E (pt) Oligonucleotidos ''antisense'' optimizados complementares de sequencias de dna metiltransferase
ATE217904T1 (de) Nukleozyme
ES2163034T3 (es) Composiciones micelares de copolimeros de bloque de polieter, para la objetivizacion de agentes biologicos como diana.
RU92016244A (ru) Производные сахарина-ингибиторы протеолитических ферментов
ATE244506T1 (de) Oligonukleotidmodulation der expression von multiwirkstoff-resistenz -assoziiertem protein
ATE294854T1 (de) Gewebespezifische und ziel-rna-spezifische ribozyme
EP1123414A4 (en) ANTISENSE MODULATION OF THE EXPRESSION OF INTEGRIN ALPHA 4
ES2085767T3 (es) Aumento de la actividad catalitica de las ribozimas por un oligonucleotido facilitador proximo.
DK1175489T3 (da) Oligonukleotider indeholdende en antisense-sekvens stabiliseret af en sekundær struktur og farmaceutiske præparater indeholdende disse oligonukleotider
WO1998032846A3 (en) Enzymatic nucleic acid treatment of diseases or conditions related to levels of c-fos
ES2088128T3 (es) Aminosulfonilureas inhibidoras de acat.
FI953363A (fi) Dimeeriblokkien synteesi ja niiden käyttö oligonukleotidien kokoamisessa
EA199901061A1 (ru) Новые соли врс-пептидов с органопротективной активностью, способ их получения и их использование в терапии
EP0902836A4 (en) HAMMER HEAD ASYMMETRIC RIBOZYMES
FR2765589B1 (fr) Fragment d'adn comprenant le promoteur de la ribulose reductase nadph specifique de yamadazyma ohmeri, vecteur le contenant et procede de preparation de proteines utilisant ce vecteur
MX9709596A (es) Adn catal ??tico.
Cieśla et al. Purification and properties of Hymenolepis diminuta and regenerating rat liver thymidylate synthases

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 625194

Country of ref document: ES