ES2068156B1 - PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME. - Google Patents

PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Info

Publication number
ES2068156B1
ES2068156B1 ES9301689A ES9301689A ES2068156B1 ES 2068156 B1 ES2068156 B1 ES 2068156B1 ES 9301689 A ES9301689 A ES 9301689A ES 9301689 A ES9301689 A ES 9301689A ES 2068156 B1 ES2068156 B1 ES 2068156B1
Authority
ES
Spain
Prior art keywords
cork
procedure
thin sections
obtaining
freezing microtome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9301689A
Other languages
Spanish (es)
Other versions
ES2068156A1 (en
Inventor
Rodriguez M Eva Barreno
Martin Simon Fos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat de Valencia
Original Assignee
Universitat de Valencia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat de Valencia filed Critical Universitat de Valencia
Priority to ES9301689A priority Critical patent/ES2068156B1/en
Publication of ES2068156A1 publication Critical patent/ES2068156A1/en
Application granted granted Critical
Publication of ES2068156B1 publication Critical patent/ES2068156B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE SECCIONES DELGADAS DEL CORCHO DE QUERCUS SUBER L. CON MICROTOMO DE CONGELACION QUE CONSISTE EN UNA PREPARACION PARTICULAR DE LAS MUESTRAS DE CORCHO Y QUE SE CARACTERIZA PORQUE SE OBTIENEN SECCIONES QUE CONTIENEN EL CRECIMIENTO RADIAL ACUMULADO EN UN PERIODO MAS O MENOS LARGO. LA METODOLOGIA EMPLEADA SE CARACTERIZA PORQUE SE CORTAN MUESTRAS DE CORCHO EN PEQUEÑAS COLUMNAS RADIALES Y SE METEN EN TUBOS DE ENSAYO CON AGUA DESTILADA CON UN HUMECTANTE EN CANTIDAD DE 4-5 GOTAS, SE MANTIENEN EN ESTUFA DURANTE 15-20 DIAS. UNA VEZ EMPAPADAS TOTALMENTE LAS COLUMNAS SE CORTAN CON EL MICROTO DE FORMA HABITUAL. LA APLICACION DE ESTE PROCEDIMIENTO PERMITE DETERMINAR LOS PARAMETROS IMPLICADOS EN LA VALORACION DE LA CALIDAD DEL CORCHO, PUDIENDO DISPONER POR PRIMERA VEZ DE CORTES QUE CONTIENEN EL CRECIMIENTO RADIAL ACUMULADO DURANTE UN PERIODO DETERMINADO, Y DETERMINAR EL ORIGEN GEOGRAFICO DE LAS MUESTRAS DEL CORCHO.PROCEDURE FOR THE OBTAINING OF THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH A FREEZING MICROTOME THAT CONSISTS OF A PARTICULAR PREPARATION OF THE CORK SAMPLES AND THAT IS CHARACTERIZED BY THE OBTAINING SECTIONS THAT CONTAIN LONGER RADIO GROWTH OR A LITTLE PERIOD . THE METHODOLOGY USED IS CHARACTERIZED BECAUSE CORK SAMPLES ARE CUT IN SMALL RADIAL COLUMNS AND PLACED IN TEST TUBES WITH DISTILLED WATER WITH A HUMIDANT IN A QUANTITY OF 4-5 DROPS, KEPT IN A STOVE FOR 15-20 DAYS. ONCE THE COLUMNS ARE FULLY SOAKED, THE CUTTINGS ARE CUT WITH THE HABITUAL WAY. THE APPLICATION OF THIS PROCEDURE ALLOWS TO DETERMINE THE PARAMETERS INVOLVED IN THE ASSESSMENT OF THE QUALITY OF THE CORK, BEING ABLE TO HAVE FOR THE FIRST TIME CUTS CONTAINING ACCUMULATED RADIAL GROWTH DURING A DETERMINED PERIOD OF THE SOURCE OF THE GEOGRAPHICAL SOURCE.

ES9301689A 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME. Expired - Fee Related ES2068156B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9301689A ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9301689A ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Publications (2)

Publication Number Publication Date
ES2068156A1 ES2068156A1 (en) 1995-04-01
ES2068156B1 true ES2068156B1 (en) 1995-11-01

Family

ID=8282700

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9301689A Expired - Fee Related ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Country Status (1)

Country Link
ES (1) ES2068156B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3257279A (en) * 1962-07-31 1966-06-21 Schain Philip Water-soluble medium for tissue infiltrating and embedding
US3624197A (en) * 1969-09-04 1971-11-30 Philip Schain Water-soluble tissue infiltrating and embedding compositions
US4404181A (en) * 1981-10-15 1983-09-13 Cambridge Chemical Products, Inc. Extended-life tissue fixative composition and method of using the same

Also Published As

Publication number Publication date
ES2068156A1 (en) 1995-04-01

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