ES2068156A1 - Procedure for obtaining thin sections of cork from Quercus suber L. with a freezing microtome - Google Patents

Procedure for obtaining thin sections of cork from Quercus suber L. with a freezing microtome

Info

Publication number
ES2068156A1
ES2068156A1 ES09301689A ES9301689A ES2068156A1 ES 2068156 A1 ES2068156 A1 ES 2068156A1 ES 09301689 A ES09301689 A ES 09301689A ES 9301689 A ES9301689 A ES 9301689A ES 2068156 A1 ES2068156 A1 ES 2068156A1
Authority
ES
Spain
Prior art keywords
cork
procedure
sections
samples
thin sections
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09301689A
Other languages
Spanish (es)
Other versions
ES2068156B1 (en
Inventor
Rodriguez M Eva Barreno
Martin Simon Fos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat de Valencia
Original Assignee
Universitat de Valencia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat de Valencia filed Critical Universitat de Valencia
Priority to ES9301689A priority Critical patent/ES2068156B1/en
Publication of ES2068156A1 publication Critical patent/ES2068156A1/en
Application granted granted Critical
Publication of ES2068156B1 publication Critical patent/ES2068156B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

Procedure for obtaining thin sections of cork from Quercus suber L. with a freezing microtome which consists in a particular preparation of the cork samples and which is characterised in that sections are obtained which contain the radial growth accumulated over a reasonably long period. The methodology employed is characterised in that samples of cork are cut in small radial columns and are placed in test-tubes with distilled water together with a quantity of 4-5 drops of a wetting agent, they are kept in an oven for 15-20 days. Once fully-soaked the columns are cut with a microtome in the normal manner. The application of this procedure permits the determination of the parameters involved in the evaluation of the quality of the cork, sections being available for the 1st time which contain the radial growth accumulated over a given period, and the ascertainment of the geographical origin of the cork samples.
ES9301689A 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME. Expired - Fee Related ES2068156B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9301689A ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9301689A ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Publications (2)

Publication Number Publication Date
ES2068156A1 true ES2068156A1 (en) 1995-04-01
ES2068156B1 ES2068156B1 (en) 1995-11-01

Family

ID=8282700

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9301689A Expired - Fee Related ES2068156B1 (en) 1993-07-28 1993-07-28 PROCEDURE FOR OBTAINING THIN SECTIONS OF THE CORK FROM QUERCUS SUBER L. WITH FREEZING MICROTOME.

Country Status (1)

Country Link
ES (1) ES2068156B1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3257279A (en) * 1962-07-31 1966-06-21 Schain Philip Water-soluble medium for tissue infiltrating and embedding
US3624197A (en) * 1969-09-04 1971-11-30 Philip Schain Water-soluble tissue infiltrating and embedding compositions
US4404181A (en) * 1981-10-15 1983-09-13 Cambridge Chemical Products, Inc. Extended-life tissue fixative composition and method of using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3257279A (en) * 1962-07-31 1966-06-21 Schain Philip Water-soluble medium for tissue infiltrating and embedding
US3624197A (en) * 1969-09-04 1971-11-30 Philip Schain Water-soluble tissue infiltrating and embedding compositions
US4404181A (en) * 1981-10-15 1983-09-13 Cambridge Chemical Products, Inc. Extended-life tissue fixative composition and method of using the same

Also Published As

Publication number Publication date
ES2068156B1 (en) 1995-11-01

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ES2068156A1 (en) Procedure for obtaining thin sections of cork from Quercus suber L. with a freezing microtome
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