ES2066732B1 - PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS. - Google Patents

PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS.

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Publication number
ES2066732B1
ES2066732B1 ES09301444A ES9301444A ES2066732B1 ES 2066732 B1 ES2066732 B1 ES 2066732B1 ES 09301444 A ES09301444 A ES 09301444A ES 9301444 A ES9301444 A ES 9301444A ES 2066732 B1 ES2066732 B1 ES 2066732B1
Authority
ES
Spain
Prior art keywords
procedure
plasmid
citrate
overexpression
citp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09301444A
Other languages
Spanish (es)
Other versions
ES2066732A1 (en
Inventor
Garcia Paloma Lopez
De Felipe Felix Lopez
Christian Magni
Diego Mendoza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Nacional de Rosario UNR
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Universidad Nacional de Rosario UNR
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Nacional de Rosario UNR, Consejo Superior de Investigaciones Cientificas CSIC filed Critical Universidad Nacional de Rosario UNR
Priority to ES09301444A priority Critical patent/ES2066732B1/en
Publication of ES2066732A1 publication Critical patent/ES2066732A1/en
Application granted granted Critical
Publication of ES2066732B1 publication Critical patent/ES2066732B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

PROCEDIMIENTO DE OBTENCION Y SOBREEXPRESION CONSTITUTIVA DE LA CITRATO PERMEASA P DE LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS METODO PARA SUBCLONAR EL GEN CITP DEL PLASMIDO LACTOCOCCAL PCIT264 EN EL PLASMIDO VECTOR DE AMPLIO ESPECTRO DE HUESPED PLS1. PARA CONSEGUIR UNA SOBREEXPRESION CONSTITUTIVA DE LA CITRATO PERMEASA SE COLOCO EL GEN CITP BAJO EL PROMOTOR DE TRANSCRIPCION DEL GEN POLA DE STREPTOCOCCUS PNEUMONIAE. MEDIANTE ESTE PROCEDIMIENTO SE HAN OBTENIDO LOS PLASMIDOS PFU1 CAPAZ DE REPLICAR EN ESCHERICHIA COLI, Y EL PLASMIDO PFL3 CAPAZ DE REPLICAR EN S. PNEUMONIAE Y L. LACTIS. LAS ESTIRPES BACTERIANAS PORTADORAS DE DICHOS PLASMIDOS ADQUIEREN LA CAPACIDAD DE TRANSPORTAR CITRATO. L. LACTIS CONTENIENDO EL PLASMIDO PFL3 SINTETIZA EL RNA MENSAJERO DE CITP A PARTIR DEL PROMOTOR POLA DE UNA FORMA CONSTITUTIVA DURANTE LAS FASES EXPONENCIAL Y ESTACIONARIA DE CRECIMIENTO BACTERIANO.PROCEDURE FOR OBTAINING AND CONSTITUTIVE OVEREXPRESSION OF CITRATE PERMEASE P FROM LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS METHOD FOR SUBCLONING THE CITP GENE OF THE LACTOCOCCAL PLASMID PCIT264 IN THE VAST PLASMID OF HUESPED SPECTRUM OF HUESP. TO OBTAIN A CONSTITUTIVE OVEREXPRESSION OF CITRATE PERMEASE, THE CITP GENE WAS PLACED UNDER THE TRANSCRIPTION PROMOTER OF THE POLA GENE OF STREPTOCOCCUS PNEUMONIAE. THROUGH THIS PROCEDURE, PLASMIDS PFU1 CAPABLE OF REPLICATION IN ESCHERICHIA COLI, AND PLASMID PFL3 CAPABLE OF REPLICATION IN S. PNEUMONIAE AND L. LACTIS. BACTERIAL STRAINS CARRYING SUCH PLASMIDS ACQUIRE THE ABILITY TO TRANSPORT CITRATE. L. LACTIS CONTAINING PLASMID PFL3 SYNTHESIZES CITP MESSENGER RNA FROM THE POLA PROMOTER IN A CONSTITUTIVE FORM DURING THE EXPONENTIAL AND STATIONARY PHASES OF BACTERIAL GROWTH.

ES09301444A 1993-06-25 1993-06-25 PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS. Expired - Fee Related ES2066732B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09301444A ES2066732B1 (en) 1993-06-25 1993-06-25 PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09301444A ES2066732B1 (en) 1993-06-25 1993-06-25 PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS.

Publications (2)

Publication Number Publication Date
ES2066732A1 ES2066732A1 (en) 1995-03-01
ES2066732B1 true ES2066732B1 (en) 1995-11-01

Family

ID=8282361

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09301444A Expired - Fee Related ES2066732B1 (en) 1993-06-25 1993-06-25 PROCEDURE FOR OBTAINING AND OVEREXPRESSION OF THE CITRATE PERMEASE P OF LACTOCOCCUS LACTIS BIOVAR DIACETILACTIS.

Country Status (1)

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ES (1) ES2066732B1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria

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Publication number Publication date
ES2066732A1 (en) 1995-03-01

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