ES2065274A1 - Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. - Google Patents
Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa.Info
- Publication number
- ES2065274A1 ES2065274A1 ES09301289A ES9301289A ES2065274A1 ES 2065274 A1 ES2065274 A1 ES 2065274A1 ES 09301289 A ES09301289 A ES 09301289A ES 9301289 A ES9301289 A ES 9301289A ES 2065274 A1 ES2065274 A1 ES 2065274A1
- Authority
- ES
- Spain
- Prior art keywords
- expression
- production
- melanin
- procedure
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PROCEDIMIENTO PARA LA PRODUCCION DE MELANINA EN CELULAS DE LA CEPA COMERCIAL DH5A DE ESCHERICHIA COLI A PARTIR DE EXPRESION EN LAS MISMAS DEL GEN ESTRUCTURAL DE LA ENZIMA TIROSINASA SE HA CONSTRUIDO UN PLASMIDO RECOMBINANTE (PME100) QUE PORTA EL GEN ESTRUCTURAL DE LA ENZIMA TIROSINASA DE LA CEPA GR4 DE RHIZOBIUM MELILOTI. ESTE PLASMIDO ES CAPAZ DE INDUCIR LA PRODUCCION DE MELANINA EN CELULAS DE E. COLI, COMO CONSECUENCIA DE LA EXPRESION DESDE EL PROMOTOR DEL GEN LACZ PRESENTE EN LA CONSTRUCCION Y APORTADO POR EL PLASMIDO PUC18. LA EXPRESION DEL GEN ES CONSTITUTIVA A PARTIR DE DICHO PROMOTOR SIN NECESIDAD DE INDUCCION PREVIA. MEDIANTE EL PROCEDIMIENTO DE LA INVENCION SE CONSIGUE LA PRODUCCION, DE UNA MANERA SENCILLA, DE CANTIDADES SUFICIENTES DE PIGMENTOS MELANICOS DE INDUDABLE APLICABILIDAD INDUSTRIAL DADA LA ENORME VARIEDAD DE PROPIEDADES QUE POSEEN.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9301289A ES2065274B1 (es) | 1993-06-11 | 1993-06-11 | Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9301289A ES2065274B1 (es) | 1993-06-11 | 1993-06-11 | Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2065274A1 true ES2065274A1 (es) | 1995-02-01 |
ES2065274B1 ES2065274B1 (es) | 1995-09-16 |
Family
ID=8282152
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9301289A Expired - Fee Related ES2065274B1 (es) | 1993-06-11 | 1993-06-11 | Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2065274B1 (es) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0363792A1 (en) * | 1988-10-03 | 1990-04-18 | Biosource Genetics Corporation | Melanin production |
WO1992000373A1 (en) * | 1990-06-29 | 1992-01-09 | Biosource Genetics Corporation | Melanin production by transformed microorganisms |
-
1993
- 1993-06-11 ES ES9301289A patent/ES2065274B1/es not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0363792A1 (en) * | 1988-10-03 | 1990-04-18 | Biosource Genetics Corporation | Melanin production |
WO1992000373A1 (en) * | 1990-06-29 | 1992-01-09 | Biosource Genetics Corporation | Melanin production by transformed microorganisms |
Non-Patent Citations (1)
Title |
---|
APPLIED AND ENVIRONMEMTAL MICROBIOLOGY vol.54, no.7, Julio 1988, Washington, USA M.T. CUBO et al.: "Melanin production by Rhizobium strains" pßgs. 1812-1817. *Todo el documento* * |
Also Published As
Publication number | Publication date |
---|---|
ES2065274B1 (es) | 1995-09-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20000707 |