ES2065274A1 - Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. - Google Patents

Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa.

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Publication number
ES2065274A1
ES2065274A1 ES09301289A ES9301289A ES2065274A1 ES 2065274 A1 ES2065274 A1 ES 2065274A1 ES 09301289 A ES09301289 A ES 09301289A ES 9301289 A ES9301289 A ES 9301289A ES 2065274 A1 ES2065274 A1 ES 2065274A1
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Spain
Prior art keywords
expression
production
melanin
procedure
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09301289A
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English (en)
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ES2065274B1 (es
Inventor
Blanco Jesus Mercado
Pascual Jose Olivares
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
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Consejo Superior de Investigaciones Cientificas CSIC
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Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9301289A priority Critical patent/ES2065274B1/es
Publication of ES2065274A1 publication Critical patent/ES2065274A1/es
Application granted granted Critical
Publication of ES2065274B1 publication Critical patent/ES2065274B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

PROCEDIMIENTO PARA LA PRODUCCION DE MELANINA EN CELULAS DE LA CEPA COMERCIAL DH5A DE ESCHERICHIA COLI A PARTIR DE EXPRESION EN LAS MISMAS DEL GEN ESTRUCTURAL DE LA ENZIMA TIROSINASA SE HA CONSTRUIDO UN PLASMIDO RECOMBINANTE (PME100) QUE PORTA EL GEN ESTRUCTURAL DE LA ENZIMA TIROSINASA DE LA CEPA GR4 DE RHIZOBIUM MELILOTI. ESTE PLASMIDO ES CAPAZ DE INDUCIR LA PRODUCCION DE MELANINA EN CELULAS DE E. COLI, COMO CONSECUENCIA DE LA EXPRESION DESDE EL PROMOTOR DEL GEN LACZ PRESENTE EN LA CONSTRUCCION Y APORTADO POR EL PLASMIDO PUC18. LA EXPRESION DEL GEN ES CONSTITUTIVA A PARTIR DE DICHO PROMOTOR SIN NECESIDAD DE INDUCCION PREVIA. MEDIANTE EL PROCEDIMIENTO DE LA INVENCION SE CONSIGUE LA PRODUCCION, DE UNA MANERA SENCILLA, DE CANTIDADES SUFICIENTES DE PIGMENTOS MELANICOS DE INDUDABLE APLICABILIDAD INDUSTRIAL DADA LA ENORME VARIEDAD DE PROPIEDADES QUE POSEEN.
ES9301289A 1993-06-11 1993-06-11 Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa. Expired - Fee Related ES2065274B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9301289A ES2065274B1 (es) 1993-06-11 1993-06-11 Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9301289A ES2065274B1 (es) 1993-06-11 1993-06-11 Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa.

Publications (2)

Publication Number Publication Date
ES2065274A1 true ES2065274A1 (es) 1995-02-01
ES2065274B1 ES2065274B1 (es) 1995-09-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES9301289A Expired - Fee Related ES2065274B1 (es) 1993-06-11 1993-06-11 Procedimiento para la produccion de melanina en celulas de la cepa comercial dh5a de escherichia coli a partir de expresion en las mismas del gen estructural de la enzima tirosinasa.

Country Status (1)

Country Link
ES (1) ES2065274B1 (es)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363792A1 (en) * 1988-10-03 1990-04-18 Biosource Genetics Corporation Melanin production
WO1992000373A1 (en) * 1990-06-29 1992-01-09 Biosource Genetics Corporation Melanin production by transformed microorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363792A1 (en) * 1988-10-03 1990-04-18 Biosource Genetics Corporation Melanin production
WO1992000373A1 (en) * 1990-06-29 1992-01-09 Biosource Genetics Corporation Melanin production by transformed microorganisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
APPLIED AND ENVIRONMEMTAL MICROBIOLOGY vol.54, no.7, Julio 1988, Washington, USA M.T. CUBO et al.: "Melanin production by Rhizobium strains" pßgs. 1812-1817. *Todo el documento* *

Also Published As

Publication number Publication date
ES2065274B1 (es) 1995-09-16

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Effective date: 20000707