ES2056458T3 - Procedimiento de estabilizacion de la hibridacion de secuencias polinucleotidicas complementarias. - Google Patents

Procedimiento de estabilizacion de la hibridacion de secuencias polinucleotidicas complementarias.

Info

Publication number
ES2056458T3
ES2056458T3 ES90904868T ES90904868T ES2056458T3 ES 2056458 T3 ES2056458 T3 ES 2056458T3 ES 90904868 T ES90904868 T ES 90904868T ES 90904868 T ES90904868 T ES 90904868T ES 2056458 T3 ES2056458 T3 ES 2056458T3
Authority
ES
Spain
Prior art keywords
hybridization
complementary
sequence
stabilization procedure
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90904868T
Other languages
English (en)
Inventor
Beno T Prieur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OPALE BIOTECHNOLOGY
Original Assignee
OPALE BIOTECHNOLOGY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OPALE BIOTECHNOLOGY filed Critical OPALE BIOTECHNOLOGY
Application granted granted Critical
Publication of ES2056458T3 publication Critical patent/ES2056458T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA INVENCION SE REFIERE A UN PROCESO DE ESTABILIZACION DE LA HIBRIDACION DE UNA PRIMERA SECUENCIA POLINUCLEOTIDICA DE ADN O DE ARN MODIFICADAS O NO, DE CONFIGURACION ANOMERICA ALFA O BETA CON UNA SEGUNDA SECUENCIA BLANCO COMPLEMENTARIA DE LA PRIMERA SECUENCIA DE ADN O DE ARN, CARACTERIZADO EN QUE UN PEPTIDO FIJADO OPCIONALMENTE SOBRE DICHA PRIMERA SECUENCIA SE COMPLEJA SOBRE LA DOBLE HELICE DE HIBRIDACION QUE FORMAN DICHAS PRIMERA Y SEGUNDA SECUENCIAS NUCLEOTICAS. LA PRESENTE INVENCION SE REFIERE TAMBIEN A CONJUGADOS PEPTIDO-POLINUCLEOTIDO UTILES PARA EL PROCESO, ASI COMO A SU APLICACION COMO SONDAS DIAGNOSTICAS O COMO AGENTES TERAPEUTICOS, EN PARTICULAR.
ES90904868T 1989-03-14 1990-03-13 Procedimiento de estabilizacion de la hibridacion de secuencias polinucleotidicas complementarias. Expired - Lifetime ES2056458T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8903303A FR2644461B1 (fr) 1989-03-14 1989-03-14 Procede de stabilisation de l'hybridation de sequences polynucleotidiques complementaires, conjugues peptides-polynucleotides utiles pour la mise en oeuvre du procede

Publications (1)

Publication Number Publication Date
ES2056458T3 true ES2056458T3 (es) 1994-10-01

Family

ID=9379652

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90904868T Expired - Lifetime ES2056458T3 (es) 1989-03-14 1990-03-13 Procedimiento de estabilizacion de la hibridacion de secuencias polinucleotidicas complementarias.

Country Status (10)

Country Link
EP (1) EP0463043B1 (es)
JP (1) JPH04506450A (es)
AT (1) ATE105871T1 (es)
AU (1) AU5337690A (es)
CA (1) CA2050913A1 (es)
DE (1) DE69009050T2 (es)
DK (1) DK0463043T3 (es)
ES (1) ES2056458T3 (es)
FR (1) FR2644461B1 (es)
WO (1) WO1990010713A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3945493A (en) * 1992-04-06 1993-11-08 University Of Medicine And Dentistry Of New Jersey Paired-ion oligonucleotides and methods for preparing same
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA

Also Published As

Publication number Publication date
ATE105871T1 (de) 1994-06-15
DK0463043T3 (da) 1994-10-10
WO1990010713A1 (fr) 1990-09-20
DE69009050D1 (de) 1994-06-23
AU5337690A (en) 1990-10-09
EP0463043B1 (fr) 1994-05-18
FR2644461B1 (fr) 1993-12-17
CA2050913A1 (fr) 1990-09-15
FR2644461A1 (fr) 1990-09-21
EP0463043A1 (fr) 1992-01-02
JPH04506450A (ja) 1992-11-12
DE69009050T2 (de) 1995-01-05

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