ES2053766T3 - Metodo para aumentar la expresion de un gen. - Google Patents
Metodo para aumentar la expresion de un gen.Info
- Publication number
- ES2053766T3 ES2053766T3 ES88310665T ES88310665T ES2053766T3 ES 2053766 T3 ES2053766 T3 ES 2053766T3 ES 88310665 T ES88310665 T ES 88310665T ES 88310665 T ES88310665 T ES 88310665T ES 2053766 T3 ES2053766 T3 ES 2053766T3
- Authority
- ES
- Spain
- Prior art keywords
- yeast
- protein
- respiratory
- gen
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 7
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 230000002950 deficient Effects 0.000 abstract 3
- 230000000241 respiratory effect Effects 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/94—Saccharomyces
- Y10S435/942—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE DESCUBREN LEVADURAS DEFICIENTES RESPIRATORIAS EXCEPTO SACCHAROMYCES CEREVISIAL AH22R-, SIENDO TRANSFORMASA DICHA LEVADURA DEFIECIENTE RESPIRATORIA CON UN ADN CONTENIENDO UN GEN QUE CODIFICA UNA PROTEINA EXTRAÑA LEVADURA Y UN METODO DE PREPARACION DE UNA PROTEINA EXTRAÑA LEVADURA DESCRITA MAS ARRIBA, QUE COMPRENDE EL CULTIVO DE LA LEVADURA Y PRODUCCION Y ACUMULACION DE LA PROTEINA EN UN CULTIVO. LA LEVADURA DEFICIENTE RESPIRATORIA DE LA PRESENTE INVENCION PUEDE PRODUCIR UNA CANTIDAD SUPERIOR DE PROTEINA QUE LA DE LA CEPA PADRE.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP28517587 | 1987-11-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2053766T3 true ES2053766T3 (es) | 1994-08-01 |
Family
ID=17688070
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88310665T Expired - Lifetime ES2053766T3 (es) | 1987-11-13 | 1988-11-11 | Metodo para aumentar la expresion de un gen. |
Country Status (6)
Country | Link |
---|---|
US (1) | US5182195A (es) |
EP (1) | EP0317209B1 (es) |
AT (1) | ATE85644T1 (es) |
CA (1) | CA1312029C (es) |
DE (1) | DE3878410T2 (es) |
ES (1) | ES2053766T3 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0358325A1 (en) * | 1988-08-10 | 1990-03-14 | Takeda Chemical Industries, Ltd. | Production of protein kinase C by yeast |
HU214698B (hu) * | 1992-04-09 | 1998-06-29 | Gyógyszerkutató Intézet Kft. | Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására |
US5342763A (en) * | 1992-11-23 | 1994-08-30 | Genentech, Inc. | Method for producing polypeptide via bacterial fermentation |
US5891669A (en) * | 1997-03-17 | 1999-04-06 | Novo Nordisk A/S, Novoalle, | Methods for producing polypeptides in respiratory-deficient cells |
WO1999049028A1 (en) | 1998-03-23 | 1999-09-30 | Genentech, Inc. | Method of selection for genes encoding secreted and transmembrane proteins |
EP1548108A4 (en) * | 2002-09-05 | 2006-05-17 | Epoch Medical Internat Inc | SCREENING METHOD FOR IDENTIFYING A SUBSTANCE HAVING PHYSIOLOGICAL ACTIVITY |
FR2857977B1 (fr) * | 2003-07-23 | 2007-10-05 | Centre Nat Rech Scient | Procede de production industrielle d'arn et systeme utile pour ladite production |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4752576A (en) * | 1984-06-14 | 1988-06-21 | Chiron Corporation | Expression of α-1 antitrypsin in yeast |
US4766068A (en) * | 1984-06-16 | 1988-08-23 | Agency Of Industrial Science And Technology | Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts |
EP0171908A3 (en) * | 1984-07-11 | 1987-07-15 | Takeda Chemical Industries, Ltd. | Hepatitis b virus surface antigen and production thereof |
JPS6170989A (ja) * | 1984-09-13 | 1986-04-11 | Takeda Chem Ind Ltd | 組み換えdnaおよびその用途 |
CA1263619A (en) * | 1984-10-09 | 1989-12-05 | Ryuji Marumoto | Dna, production and use thereof |
JPS61158793A (ja) * | 1984-11-14 | 1986-07-18 | Takeda Chem Ind Ltd | ヒト・リゾチ−ムの合成遺伝子 |
JPH0745514B2 (ja) * | 1985-08-20 | 1995-05-17 | 武田薬品工業株式会社 | 新規蛋白質およびその製造法 |
WO1987003298A1 (en) * | 1985-11-21 | 1987-06-04 | The Australian National University | Method for genetically marking yeast |
TW205070B (es) * | 1986-06-30 | 1993-05-01 | Takeda Pharm Industry Co Ltd |
-
1988
- 1988-11-09 US US07/269,140 patent/US5182195A/en not_active Expired - Fee Related
- 1988-11-10 CA CA000582701A patent/CA1312029C/en not_active Expired - Lifetime
- 1988-11-11 AT AT88310665T patent/ATE85644T1/de not_active IP Right Cessation
- 1988-11-11 ES ES88310665T patent/ES2053766T3/es not_active Expired - Lifetime
- 1988-11-11 DE DE8888310665T patent/DE3878410T2/de not_active Expired - Fee Related
- 1988-11-11 EP EP88310665A patent/EP0317209B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5182195A (en) | 1993-01-26 |
ATE85644T1 (de) | 1993-02-15 |
EP0317209A3 (en) | 1989-10-18 |
EP0317209A2 (en) | 1989-05-24 |
CA1312029C (en) | 1992-12-29 |
EP0317209B1 (en) | 1993-02-10 |
DE3878410T2 (de) | 1993-06-03 |
DE3878410D1 (de) | 1993-03-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
PT88479A (pt) | Metodo para cultura de celulas recombinantes | |
BR8200191A (pt) | Celula viva renina preprorrenina prorrenina processo para obter expressao de preporrenina prorrenina e renina gene de prorrenina preprorrenina e renina e coli material dna recombinador saccharomyces cerevisiae polipetidio processo para exprimir um gene | |
ATE77104T1 (de) | Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute. | |
ES2122955T3 (es) | Adn que codifica proteinas que se unen a la il-1 humana. | |
EP0537173A4 (en) | Cloning by complementation and related processes | |
ES2109238T3 (es) | Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. | |
DE68929115D1 (de) | Verfahren zur Herstellung von menschlichem Serumalbumin in Hefe | |
PT83761A (en) | Method for producing mammalian interleukin-4 | |
ATE70308T1 (de) | Hybridpartikel-immunogene. | |
ES8202362A1 (es) | Procedimiento para preparar un acido desoxi-ribonucleico (adn) recombinante | |
ATE123065T1 (de) | Proteine und deren herstellung. | |
ATE48643T1 (de) | Verfahren zur herstellung von glucagon oder dessen derivaten in einem transformierten hefestamm. | |
ATE353953T1 (de) | Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion | |
ES2053766T3 (es) | Metodo para aumentar la expresion de un gen. | |
EP0269388A3 (en) | Method for the evaluation of oral microbes | |
ATE155170T1 (de) | Fermentationsprozess zur herstellung von pyrimidin | |
ATE103989T1 (de) | Monoklonale antikoerper gegen e7-protein des humanen papillomvirus typ 16, verfahren zu ihrer herstellung sowie ihre verwendung. | |
EP0311456A3 (en) | Monoclonal antibody to okadaic acids, process for producing the monoclonal antibody, assay reagent for assaying okadaic acids using the monoclonal antibody, and assay method | |
SE9301583D0 (sv) | Yeast strain and methods for expressing heterologous proteins in yeast | |
NZ515762A (en) | Method of producing a heparin-binding protein by expressing it in a mammalian cell that can be cultured in anaerobic conditions | |
PT83368A (en) | Process for propagating microorganisms in vivo by using cell lines which lack human class ii histocompatibility antigens | |
Del Giudice et al. | Synthesis of mitochondrial DNA during the cell cycle of the petite negative yeast Schizosaccharomyces pombe | |
FI871584A (fi) | Uppbyggande av nya -galaktosidas producerande jaeststammar och foerfaranden foer industriellt utnyttjande av dylika jaeststammar. | |
Barsomian et al. | Endo F-free PNGase | |
AU2278392A (en) | Method for producing recombinant proteins and host cells used therein |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 317209 Country of ref document: ES |