ES2049618B1 - DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. - Google Patents

DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

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Publication number
ES2049618B1
ES2049618B1 ES09102521A ES9102521A ES2049618B1 ES 2049618 B1 ES2049618 B1 ES 2049618B1 ES 09102521 A ES09102521 A ES 09102521A ES 9102521 A ES9102521 A ES 9102521A ES 2049618 B1 ES2049618 B1 ES 2049618B1
Authority
ES
Spain
Prior art keywords
trypanosoma cruzi
classification
diagnostic method
species
parasite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES09102521A
Other languages
Spanish (es)
Other versions
ES2049618A1 (en
Inventor
Carlos Alonso
Jose Maria Requena
Manuel Carlos Lopez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES09102521A priority Critical patent/ES2049618B1/en
Publication of ES2049618A1 publication Critical patent/ES2049618A1/en
Application granted granted Critical
Publication of ES2049618B1 publication Critical patent/ES2049618B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

METODO DE DIAGNOSTICO Y CLASIFICACION DE ESPECIES DE TRYPANOSOMA CRUZI. A TRAVES DE UNA SECUENCIA DE NUCLEOTIDOS ES POSIBLE PRODUCIR POR SINTESIS QUIMICA FRAGMENTOS DE OLIGONUCLEOTIDOS DE ESTRUCTURA PARCIAL IDENTICA. ESTOS FRAGMENTOS CONSTITUYEN LAS SONDAS QUE SE UTILIZAN COMO CEBADORES PARA LA AMPLIFICACION DE UN FRAGMENTO MAYOR DEL DNA DEL PARASITO COMPRENDIDO ENTRE LOS OLIGONUCLEOTIDOS SINTETIZADOS. LA DETECCION DE DNA AMPLIFICADO ES UNA DEMOSTRACION DIRECTA DE LA PRESENCIA DEL PARASITO EN LA MUESTRA QUE SE ANALIZA. LA SECUENCIA SE UTILIZA PARA LA DETECCION DE TRYPANOSOMA CRUZI POR PCR (REACCION DE LA POLIMERACION EN CADENA) Y PARA LA CLASIFICACION DE ESPECIES Y VARIEDADES DE TRYPANOSOMA CRUZI.DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. THROUGH A SEQUENCE OF NUCLEOTIDES IT IS POSSIBLE TO PRODUCE BY CHEMICAL SYNTHESIS FRAGMENTS OF OLIGONUCLEOTIDES OF IDENTIFIC PARTIAL STRUCTURE. THESE FRAGMENTS CONSTITUTE THE PROBES THAT ARE USED AS PRIMERS FOR THE AMPLIFICATION OF A MAJOR FRAGMENT OF THE DNA OF THE PARASITE INCLUDED AMONG THE SYNTHESIZED OLIGONUCLEOTIDES. THE DETECTION OF AMPLIFIED DNA IS A DIRECT DEMONSTRATION OF THE PRESENCE OF THE PARASITE IN THE SAMPLE ANALYZED. THE SEQUENCE IS USED FOR THE DETECTION OF TRYPANOSOMA CRUZI BY PCR (REACTION OF CHAIN POLYMERATION) AND FOR THE CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES AND VARIETIES.

ES09102521A 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. Expired - Lifetime ES2049618B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09102521A ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09102521A ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Publications (2)

Publication Number Publication Date
ES2049618A1 ES2049618A1 (en) 1994-04-16
ES2049618B1 true ES2049618B1 (en) 1994-11-01

Family

ID=8274151

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09102521A Expired - Lifetime ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Country Status (1)

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ES (1) ES2049618B1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (en) * 1981-10-16 1983-07-11 Orion Yhtymae Oy MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER
NO843838L (en) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc PROCEDURE FOR DETECTING NUCLEIC ACID
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
AU5645690A (en) * 1989-05-05 1990-11-29 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
AU640745B2 (en) * 1990-03-30 1993-09-02 Astra Aktiebolag A novel procedure for the detection of pathogens using dna probes

Also Published As

Publication number Publication date
ES2049618A1 (en) 1994-04-16

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