ES2049618A1 - Diagnostic and classification method for trypanosome cruzi species - uses synthetic oligo-nucleotide(s) for amplification of DNA fragments of the parasite - Google Patents

Diagnostic and classification method for trypanosome cruzi species - uses synthetic oligo-nucleotide(s) for amplification of DNA fragments of the parasite

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Publication number
ES2049618A1
ES2049618A1 ES9102521A ES9102521A ES2049618A1 ES 2049618 A1 ES2049618 A1 ES 2049618A1 ES 9102521 A ES9102521 A ES 9102521A ES 9102521 A ES9102521 A ES 9102521A ES 2049618 A1 ES2049618 A1 ES 2049618A1
Authority
ES
Spain
Prior art keywords
parasite
amplification
detection
fragments
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9102521A
Other languages
Spanish (es)
Other versions
ES2049618B1 (en
Inventor
Alonso
Requena
Lopez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES09102521A priority Critical patent/ES2049618B1/en
Publication of ES2049618A1 publication Critical patent/ES2049618A1/en
Application granted granted Critical
Publication of ES2049618B1 publication Critical patent/ES2049618B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Abstract

By means of a nucleotide sequence, it is possible to produce by chemical synthesis fragments of oligonucleotides of identical partial structure. These fragments constitute the probes used as starters for the amplification of a larger fragment of DNA of the parasite included among the synthesised oligonucleotides. The detection of amplified DNA is direct evidence of the presence of the parasite in the sample being analysed. The sequence is used for the detection of Trypanosome cruzi by PCR (polymerisation chain reaction) and for the classification of species and varieties of Trypanosome cruzi.< IMAGE >Using a sequence of nucleotides fragments of oligonucleotides with structure partially identical to those in the parasite can be synthesised. These fragments form probes which are used as bait for amplification of a larger DNA fragment of the parasite included between the synthetic oligonucleotides. Detection of the amplified DNA is direct evidence of the presence of the parasite in a sample. The sequence is used for detection of trypanosome cruzi by chain polymerisation reaction and for classification of the species and varieties. The nucleotide sequence is stated.
ES09102521A 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. Expired - Lifetime ES2049618B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09102521A ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09102521A ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Publications (2)

Publication Number Publication Date
ES2049618A1 true ES2049618A1 (en) 1994-04-16
ES2049618B1 ES2049618B1 (en) 1994-11-01

Family

ID=8274151

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09102521A Expired - Lifetime ES2049618B1 (en) 1991-11-13 1991-11-13 DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES.

Country Status (1)

Country Link
ES (1) ES2049618B1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001459A1 (en) * 1981-10-16 1983-04-28 Ranki, Tuula, Marjut A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids
EP0139489A2 (en) * 1983-09-26 1985-05-02 Ortho Diagnostic Systems Inc. Sandwich hybridization method for nucleic acid detection
WO1990007582A1 (en) * 1989-01-05 1990-07-12 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
WO1990013668A1 (en) * 1989-05-05 1990-11-15 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
GB2242904A (en) * 1990-03-30 1991-10-16 Astra Ab Detection of pathogens using DNA probes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001459A1 (en) * 1981-10-16 1983-04-28 Ranki, Tuula, Marjut A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids
EP0139489A2 (en) * 1983-09-26 1985-05-02 Ortho Diagnostic Systems Inc. Sandwich hybridization method for nucleic acid detection
WO1990007582A1 (en) * 1989-01-05 1990-07-12 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
WO1990013668A1 (en) * 1989-05-05 1990-11-15 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
GB2242904A (en) * 1990-03-30 1991-10-16 Astra Ab Detection of pathogens using DNA probes

Also Published As

Publication number Publication date
ES2049618B1 (en) 1994-11-01

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