ES2049618A1 - Diagnostic and classification method for trypanosome cruzi species - uses synthetic oligo-nucleotide(s) for amplification of DNA fragments of the parasite - Google Patents
Diagnostic and classification method for trypanosome cruzi species - uses synthetic oligo-nucleotide(s) for amplification of DNA fragments of the parasiteInfo
- Publication number
- ES2049618A1 ES2049618A1 ES9102521A ES9102521A ES2049618A1 ES 2049618 A1 ES2049618 A1 ES 2049618A1 ES 9102521 A ES9102521 A ES 9102521A ES 9102521 A ES9102521 A ES 9102521A ES 2049618 A1 ES2049618 A1 ES 2049618A1
- Authority
- ES
- Spain
- Prior art keywords
- parasite
- amplification
- detection
- fragments
- oligonucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
By means of a nucleotide sequence, it is possible to produce by chemical synthesis fragments of oligonucleotides of identical partial structure. These fragments constitute the probes used as starters for the amplification of a larger fragment of DNA of the parasite included among the synthesised oligonucleotides. The detection of amplified DNA is direct evidence of the presence of the parasite in the sample being analysed. The sequence is used for the detection of Trypanosome cruzi by PCR (polymerisation chain reaction) and for the classification of species and varieties of Trypanosome cruzi.< IMAGE >Using a sequence of nucleotides fragments of oligonucleotides with structure partially identical to those in the parasite can be synthesised. These fragments form probes which are used as bait for amplification of a larger DNA fragment of the parasite included between the synthetic oligonucleotides. Detection of the amplified DNA is direct evidence of the presence of the parasite in a sample. The sequence is used for detection of trypanosome cruzi by chain polymerisation reaction and for classification of the species and varieties. The nucleotide sequence is stated.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09102521A ES2049618B1 (en) | 1991-11-13 | 1991-11-13 | DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09102521A ES2049618B1 (en) | 1991-11-13 | 1991-11-13 | DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2049618A1 true ES2049618A1 (en) | 1994-04-16 |
ES2049618B1 ES2049618B1 (en) | 1994-11-01 |
Family
ID=8274151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09102521A Expired - Lifetime ES2049618B1 (en) | 1991-11-13 | 1991-11-13 | DIAGNOSTIC METHOD AND CLASSIFICATION OF TRYPANOSOMA CRUZI SPECIES. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2049618B1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1983001459A1 (en) * | 1981-10-16 | 1983-04-28 | Ranki, Tuula, Marjut | A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids |
EP0139489A2 (en) * | 1983-09-26 | 1985-05-02 | Ortho Diagnostic Systems Inc. | Sandwich hybridization method for nucleic acid detection |
WO1990007582A1 (en) * | 1989-01-05 | 1990-07-12 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
WO1990013668A1 (en) * | 1989-05-05 | 1990-11-15 | Lifecodes Corporation | Method for genetic analysis of a nucleic acid sample |
GB2242904A (en) * | 1990-03-30 | 1991-10-16 | Astra Ab | Detection of pathogens using DNA probes |
-
1991
- 1991-11-13 ES ES09102521A patent/ES2049618B1/en not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1983001459A1 (en) * | 1981-10-16 | 1983-04-28 | Ranki, Tuula, Marjut | A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids |
EP0139489A2 (en) * | 1983-09-26 | 1985-05-02 | Ortho Diagnostic Systems Inc. | Sandwich hybridization method for nucleic acid detection |
WO1990007582A1 (en) * | 1989-01-05 | 1990-07-12 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
WO1990013668A1 (en) * | 1989-05-05 | 1990-11-15 | Lifecodes Corporation | Method for genetic analysis of a nucleic acid sample |
GB2242904A (en) * | 1990-03-30 | 1991-10-16 | Astra Ab | Detection of pathogens using DNA probes |
Also Published As
Publication number | Publication date |
---|---|
ES2049618B1 (en) | 1994-11-01 |
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