ES2048639A1 - Diagnostic culture medium for S. agalactiae - contains folate inhibitor, glucose, phosphate buffer, magnesium and pyruvate - Google Patents

Diagnostic culture medium for S. agalactiae - contains folate inhibitor, glucose, phosphate buffer, magnesium and pyruvate

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Publication number
ES2048639A1
ES2048639A1 ES9102833A ES9102833A ES2048639A1 ES 2048639 A1 ES2048639 A1 ES 2048639A1 ES 9102833 A ES9102833 A ES 9102833A ES 9102833 A ES9102833 A ES 9102833A ES 2048639 A1 ES2048639 A1 ES 2048639A1
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Prior art keywords
agalactiae
glucose
pyruvate
culture medium
medium
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ES9102833A
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Spanish (es)
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ES2048639B1 (en
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Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

New culture medium for detecting S. agalactiae in human and animal clinical samples characterised by the inclusion of methotrexate (folate inhibitor), glucose (as mono or oligosaccharide), a phosphate-MOPS, Mg<++> and pyruvate buffer.Tubes of this medium for use in tubes with or without agar as a transport-incubation medium, for direct inoculation of the samples from samplings, with incubation starting immediately to accelerate and facilitate rapid diagnosis of S. agalactiae by visual detection, spectrophotometric detection or any other form of physical or chemical detection of its orange or red pigment.Incorporated in the culture medium are metrotrexate (folate inhibitor), glucose (as monooligo-saccharide), MOPS phosphate buffer, Mg and pyruvate. The medium is used on tubes with or without agar, and detection of streptococcus agalactiae is by visual or spectro-photometric examination for the orange or red pigment produced.
ES9102833A 1991-12-19 1991-12-19 DIAGNOSTIC CULTURE MEDIA OF STREPTOCOCCUS AGALACTIAE BY DETECTION OF ITS PIGMENT AND WHICH ACTIVATES THE FORMATION OF THIS PIGMENT BY METROTREXATE AND / OR OTHER INHIBITORS OF THE FOLATE PATH. Expired - Fee Related ES2048639B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9102833A ES2048639B1 (en) 1991-12-19 1991-12-19 DIAGNOSTIC CULTURE MEDIA OF STREPTOCOCCUS AGALACTIAE BY DETECTION OF ITS PIGMENT AND WHICH ACTIVATES THE FORMATION OF THIS PIGMENT BY METROTREXATE AND / OR OTHER INHIBITORS OF THE FOLATE PATH.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9102833A ES2048639B1 (en) 1991-12-19 1991-12-19 DIAGNOSTIC CULTURE MEDIA OF STREPTOCOCCUS AGALACTIAE BY DETECTION OF ITS PIGMENT AND WHICH ACTIVATES THE FORMATION OF THIS PIGMENT BY METROTREXATE AND / OR OTHER INHIBITORS OF THE FOLATE PATH.

Publications (2)

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ES2048639A1 true ES2048639A1 (en) 1994-03-16
ES2048639B1 ES2048639B1 (en) 1994-11-16

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ES9102833A Expired - Fee Related ES2048639B1 (en) 1991-12-19 1991-12-19 DIAGNOSTIC CULTURE MEDIA OF STREPTOCOCCUS AGALACTIAE BY DETECTION OF ITS PIGMENT AND WHICH ACTIVATES THE FORMATION OF THIS PIGMENT BY METROTREXATE AND / OR OTHER INHIBITORS OF THE FOLATE PATH.

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2088827A1 (en) * 1995-02-23 1996-09-16 Fraile Manuel De La Rosa Culture medium for Streptococcus agalactiae which activates the formation of pigment by amylases and/or [G(&alpha1-4)G] (n>2) glucooligosaccharides added as chemical compounds, as polysaccharide hydrolysates or generated in the same medium using enzymes and polysaccharides.

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778657B1 (en) 2004-04-26 2014-07-15 Hardy Diagnostics Culture medium for cultivation of microorganisms
US8313938B1 (en) 2004-04-26 2012-11-20 Hardy Diagnostics Culture medium for cultivation of microorganisms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J. CLIN. MICROBIOL., Vol. 4, 1976, K. MERRIT and N.J. JACOBS: "Improved medium for detecting pigment production by group B streptococci", pßg. 379-380 *
J. CLIN. PATHOL., Vol. 36, 1983, M.A. NOBLE, J.M. BERT and A.B. WEST: "Detection and identification of group B streptococci by use of pigment formation", pßg. 350-352 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2088827A1 (en) * 1995-02-23 1996-09-16 Fraile Manuel De La Rosa Culture medium for Streptococcus agalactiae which activates the formation of pigment by amylases and/or [G(&alpha1-4)G] (n>2) glucooligosaccharides added as chemical compounds, as polysaccharide hydrolysates or generated in the same medium using enzymes and polysaccharides.

Also Published As

Publication number Publication date
ES2048639B1 (en) 1994-11-16

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