ES2088827A1 - Culture medium for Streptococcus agalactiae which activates the formation of pigment by amylases and/or [G(&alpha1-4)G] (n>2) glucooligosaccharides added as chemical compounds, as polysaccharide hydrolysates or generated in the same medium using enzymes and polysaccharides. - Google Patents
Culture medium for Streptococcus agalactiae which activates the formation of pigment by amylases and/or [G(&alpha1-4)G] (n>2) glucooligosaccharides added as chemical compounds, as polysaccharide hydrolysates or generated in the same medium using enzymes and polysaccharides.Info
- Publication number
- ES2088827A1 ES2088827A1 ES09500365A ES9500365A ES2088827A1 ES 2088827 A1 ES2088827 A1 ES 2088827A1 ES 09500365 A ES09500365 A ES 09500365A ES 9500365 A ES9500365 A ES 9500365A ES 2088827 A1 ES2088827 A1 ES 2088827A1
- Authority
- ES
- Spain
- Prior art keywords
- pigment
- amylases
- formation
- added
- chemical compounds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Culture medium for the detection of S. agalactiae in clinical samples characterized by incorporation in media containing starch or its amylase and/or maltooligosaccharide fractions (with 3 or more glucopyranosyl units linked by [alpha]1-4 bonds) to activate the formation of pigment. The maltooligosaccharides can be added as chemical compounds, as glucopolysaccharide hydrolysates or as glucopolysaccharides and hydrolytic enzymes (amylases, glucoamylases or phosphorylases). This avoids the addition of animal serum, and these media can be used similarly to those which employ serum as media for detection and transport/incubation, for direct inoculation of samples, their incubation beginning immediately, assisting the rapid diagnosis of S. agalactiae through the detection of its orange pigment.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9500365A ES2088827B1 (en) | 1995-02-23 | 1995-02-23 | CULTIVATION MEDIUM FOR STREPTOCOCCUS AGALACTIAE WHICH ACTIVATES PIGMENT FORMATION BY AMYLASES AND / OR GLUCOOLIGOSACCHARIDES (G (81-4) G) (N 2), ADDED AS CHEMICAL COMPOUNDS, AS HYDROLYZES OF POLYSACCHARIDES OR GENERATED IN MISTAZES POLYSACCHARIDES. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9500365A ES2088827B1 (en) | 1995-02-23 | 1995-02-23 | CULTIVATION MEDIUM FOR STREPTOCOCCUS AGALACTIAE WHICH ACTIVATES PIGMENT FORMATION BY AMYLASES AND / OR GLUCOOLIGOSACCHARIDES (G (81-4) G) (N 2), ADDED AS CHEMICAL COMPOUNDS, AS HYDROLYZES OF POLYSACCHARIDES OR GENERATED IN MISTAZES POLYSACCHARIDES. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2088827A1 true ES2088827A1 (en) | 1996-09-16 |
ES2088827B1 ES2088827B1 (en) | 1997-05-16 |
Family
ID=8289570
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9500365A Expired - Fee Related ES2088827B1 (en) | 1995-02-23 | 1995-02-23 | CULTIVATION MEDIUM FOR STREPTOCOCCUS AGALACTIAE WHICH ACTIVATES PIGMENT FORMATION BY AMYLASES AND / OR GLUCOOLIGOSACCHARIDES (G (81-4) G) (N 2), ADDED AS CHEMICAL COMPOUNDS, AS HYDROLYZES OF POLYSACCHARIDES OR GENERATED IN MISTAZES POLYSACCHARIDES. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2088827B1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000068417A1 (en) * | 1999-05-11 | 2000-11-16 | Rosa Fraile Manuel De | Dehydrated immediate reconstruction culture medium to identify group b streptococci streptococcus agalactiae by the detection of their pigment |
ES2153317A1 (en) * | 1999-03-04 | 2001-02-16 | Rosa Fraile Manuel De | Diagnosis of Streptococcus via pigment detection comprises avoidance of anaerobic incubation, for detection of Streptococcus agalactiae |
WO2004063392A2 (en) | 2003-01-10 | 2004-07-29 | Centro Nacional De Biopreparados | Selective culture medium for the isolation and/or detection of species in the streptococcus genus |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8778657B1 (en) | 2004-04-26 | 2014-07-15 | Hardy Diagnostics | Culture medium for cultivation of microorganisms |
US8313938B1 (en) | 2004-04-26 | 2012-11-20 | Hardy Diagnostics | Culture medium for cultivation of microorganisms |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2048639A1 (en) * | 1991-12-19 | 1994-03-16 | Rosa Fraile | Diagnostic culture medium for S. agalactiae - contains folate inhibitor, glucose, phosphate buffer, magnesium and pyruvate |
-
1995
- 1995-02-23 ES ES9500365A patent/ES2088827B1/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2048639A1 (en) * | 1991-12-19 | 1994-03-16 | Rosa Fraile | Diagnostic culture medium for S. agalactiae - contains folate inhibitor, glucose, phosphate buffer, magnesium and pyruvate |
Non-Patent Citations (2)
Title |
---|
BASE DE DATOS WPIL en Questel, n‘ acceso 88-012417, 1988, Derwent Publications Ltd., London, GB & SE 452893 B (DERMACI AB) 21.12.87 * Resumen * * |
DAVIS et al., "Polysaccharase Activity in Streptococcus agalactiae (Group B Streptococci)" Journal of General Microbiology. 1982. Vol. 128, pßginas 1381-1384 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2153317A1 (en) * | 1999-03-04 | 2001-02-16 | Rosa Fraile Manuel De | Diagnosis of Streptococcus via pigment detection comprises avoidance of anaerobic incubation, for detection of Streptococcus agalactiae |
WO2000068417A1 (en) * | 1999-05-11 | 2000-11-16 | Rosa Fraile Manuel De | Dehydrated immediate reconstruction culture medium to identify group b streptococci streptococcus agalactiae by the detection of their pigment |
WO2004063392A2 (en) | 2003-01-10 | 2004-07-29 | Centro Nacional De Biopreparados | Selective culture medium for the isolation and/or detection of species in the streptococcus genus |
Also Published As
Publication number | Publication date |
---|---|
ES2088827B1 (en) | 1997-05-16 |
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