ES2038614T3 - 7-(fenilacetamido)-4-alquilcumarina, procedimiento para su preparacion asi como su empleo en procedimiento para la determinacion de la actividad de hidrolasas, especialmente de penicilina-g-acilasa. - Google Patents
7-(fenilacetamido)-4-alquilcumarina, procedimiento para su preparacion asi como su empleo en procedimiento para la determinacion de la actividad de hidrolasas, especialmente de penicilina-g-acilasa.Info
- Publication number
- ES2038614T3 ES2038614T3 ES198787106175T ES87106175T ES2038614T3 ES 2038614 T3 ES2038614 T3 ES 2038614T3 ES 198787106175 T ES198787106175 T ES 198787106175T ES 87106175 T ES87106175 T ES 87106175T ES 2038614 T3 ES2038614 T3 ES 2038614T3
- Authority
- ES
- Spain
- Prior art keywords
- procedure
- hydrolases
- penicillin
- activity
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/06—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2
- C07D311/08—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring
- C07D311/16—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 7
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/20—Coumarin derivatives
- C12Q2337/22—7-Amino-4-methylcoumarin, i.e. AMC, MCA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/20—Coumarin derivatives
- C12Q2337/24—7-Amino-4-trifluoromethylcoumarin, i.e. AFC
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Pyrane Compounds (AREA)
Abstract
LAS 4-ALQUIL-CUMARINILAMIDAS DEL ACIDO 7-FENILACETICO DE LA FORMULA (I) EN LA QUE R SIGNIFICA ALQUILO DE 1 A 4 CARBONOS, ESPECIALMENTE, DE 1 A 2 CARBONOS, EL CUAL, EN SU CASO, ESTA SUSTITUIDO CON UNO O MAS ATOMOS DE HALOGENO, ESPECIALMENTE ATOMOS DE FLUOR, ESPECIALMENTE METILO O TRIFLUOROMETILO, REPRESENTAN SUSTRATOA ADECUADOS PARA LA DETERMINACION FLUOROMETRICA DE LA ACTIVIDAD DE LAS HIDROLASAS, ESPECIALMENTE DE LA PENICILINA-G-ACILASA, PORQUE EN LA REACCION ENZIMATICA PROPORCIONAN PRODUCTOS DE ESCISION QUE SE DIFERENCIAN MUCHO DE LOS DEL SUSTRATO POR SUS PROPIEDADES DE FLUORESCENCIA. PUEDEN EMPLEARSE EN EL PROCEDIMIENTO PARA LA DETERMINACION FLUOROMETRICA DE LA ACTIVIDAD DE LAS HIDROLASAS, ESPECIALMENTE, DE LA PENICILINA-G-ACILASA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19863614647 DE3614647A1 (de) | 1986-04-30 | 1986-04-30 | 7-phenylessigsaeure-4-alkyl-coumarinylamide, verfahren zu ihrer herstellung sowie ihre verwendung in verfahren zur fluorometrischen bestimmung der aktivitaet von hydrolasen, insbesondere von penicillin-g-acylase |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2038614T3 true ES2038614T3 (es) | 1993-08-01 |
Family
ID=6299870
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES198787106175T Expired - Lifetime ES2038614T3 (es) | 1986-04-30 | 1987-04-28 | 7-(fenilacetamido)-4-alquilcumarina, procedimiento para su preparacion asi como su empleo en procedimiento para la determinacion de la actividad de hidrolasas, especialmente de penicilina-g-acilasa. |
Country Status (7)
Country | Link |
---|---|
US (1) | US4777269A (es) |
EP (1) | EP0247373B1 (es) |
JP (1) | JPS62267281A (es) |
AT (1) | ATE60330T1 (es) |
DE (2) | DE3614647A1 (es) |
ES (1) | ES2038614T3 (es) |
GR (1) | GR3001701T3 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3834597A1 (de) * | 1988-10-11 | 1990-04-12 | Hoechst Ag | Neues cumarinderivat, verfahren zu seiner herstellung und seine verwendung |
US5055594A (en) * | 1990-07-19 | 1991-10-08 | Becton, Dickinson And Company | Fluorogenic trypotophanase substrates |
US5444042A (en) * | 1990-12-28 | 1995-08-22 | Cortex Pharmaceuticals | Method of treatment of neurodegeneration with calpain inhibitors |
FR2677023B1 (fr) * | 1991-05-30 | 1994-02-25 | Eurobio Laboratoires | Derives de coumarine hydrosolubles, leur preparation et leur utilisation comme substrat d'enzyme. |
US5576424A (en) * | 1991-08-23 | 1996-11-19 | Molecular Probes, Inc. | Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
DE69229174T2 (de) * | 1991-08-23 | 1999-09-16 | Molecular Probes Inc | Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen |
USRE38723E1 (en) * | 1991-08-23 | 2005-04-12 | Molecular Probes, Inc. | Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
FR2703683B1 (fr) * | 1993-04-08 | 1995-07-07 | Seractec | Dérivés d'aminocoumarines hydrophobes, et leur utilisation comme substrats d'enzymes protéolytiques ou pour la préparation de tels substrats. |
CA2514564A1 (en) * | 2003-01-31 | 2005-07-26 | Promega Corporation | Covalent tethering of functional groups to proteins |
CN101586146B (zh) * | 2008-05-23 | 2012-02-08 | 中国科学院沈阳应用生态研究所 | 一种检测土壤纤维素酶活性的分析方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2881186A (en) * | 1954-11-12 | 1959-04-07 | Geigy Ag J R | New derivatives of 7-aminocoumarin |
DE1293160B (de) * | 1964-03-07 | 1969-04-24 | Bayer Ag | Verfahren zur Herstellung von Cumarinverbindungen |
US3741876A (en) * | 1970-08-06 | 1973-06-26 | Mason Res Inst Inc | Method of measuring enzyme reaction rates |
LU75688A1 (es) * | 1976-08-31 | 1978-04-13 | ||
JPS5524147A (en) * | 1978-08-10 | 1980-02-21 | Ajinomoto Co Inc | Peptide derivative |
US4294923A (en) * | 1979-04-23 | 1981-10-13 | Smith Robert E | Substrates and method for determining enzymes |
US4409140A (en) * | 1979-04-23 | 1983-10-11 | Smith Robert E | Substrates and method for determining enzymes |
FI63064C (fi) * | 1980-04-09 | 1983-04-11 | Ksv Chemicals Oy | Foerfarande foer fluorometrisk bestaemning av aktiviteten av fettspjaelkande enzymer och medel foer att genomfoera foerfarandet |
US4388233A (en) * | 1981-05-15 | 1983-06-14 | The Regents Of The University Of California | Synthetic substrates for enzyme analysis |
JPH108974A (ja) * | 1996-06-25 | 1998-01-13 | Yamaha Motor Co Ltd | 内燃エンジンのコンロッド過給装置 |
-
1986
- 1986-04-30 DE DE19863614647 patent/DE3614647A1/de active Granted
-
1987
- 1987-04-27 JP JP62102136A patent/JPS62267281A/ja active Pending
- 1987-04-28 DE DE8787106175T patent/DE3767564D1/de not_active Expired - Fee Related
- 1987-04-28 EP EP87106175A patent/EP0247373B1/de not_active Expired - Lifetime
- 1987-04-28 ES ES198787106175T patent/ES2038614T3/es not_active Expired - Lifetime
- 1987-04-28 AT AT87106175T patent/ATE60330T1/de not_active IP Right Cessation
- 1987-04-30 US US07/044,260 patent/US4777269A/en not_active Expired - Fee Related
-
1991
- 1991-04-01 GR GR91400413T patent/GR3001701T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
GR3001701T3 (en) | 1992-11-23 |
EP0247373B1 (de) | 1991-01-23 |
US4777269A (en) | 1988-10-11 |
DE3767564D1 (de) | 1991-02-28 |
ATE60330T1 (de) | 1991-02-15 |
EP0247373A3 (en) | 1989-05-31 |
JPS62267281A (ja) | 1987-11-19 |
EP0247373A2 (de) | 1987-12-02 |
DE3614647C2 (es) | 1988-07-07 |
DE3614647A1 (de) | 1987-11-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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