ES2025654T3 - Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando. - Google Patents

Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando.

Info

Publication number
ES2025654T3
ES2025654T3 ES198787303661T ES87303661T ES2025654T3 ES 2025654 T3 ES2025654 T3 ES 2025654T3 ES 198787303661 T ES198787303661 T ES 198787303661T ES 87303661 T ES87303661 T ES 87303661T ES 2025654 T3 ES2025654 T3 ES 2025654T3
Authority
ES
Spain
Prior art keywords
league
fluid
catalyst
modulator
indicator reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787303661T
Other languages
English (en)
Inventor
Michael E. Perlman
Susan A. Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2025654T3 publication Critical patent/ES2025654T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/821—Chemistry: analytical and immunological testing involving complement factors or complement systems

Abstract

UN METODO DE INMUNOENSAYO PARA UN LIGANDO SOSPECHOSO DE ESTAR PRESENTE EN UN FLUIDO INCLUYE EL USO DE UN ENZIMA, UN CATALIZADOR ION METALICO PARA UNA REACCION DE INDICADOR Y UN MODULADOR DE BLOQUEO PARA EL CATALIZADOR. EL LIGANDO PRESENTE EN EL FLUIDO SE UNE A UN ANTILIGANDO. LA FRACCION DE ENLACE RESULTANTE ACTIVA EL ENZIMA PARA DESBLOQUEAR EL MODULADOR. EL MODULADOR LIBRE ACTIVA O INHIBE EL CATALIZADOR, MODULANDO ASI LAS VELOCIDADES DE UNA REACCION INDICADOR ENTRE UN SUBSTRATO Y UN REACTIVO REDOX. LA PRESENCIA O AUSENCIA DEL LIGANDO EN EL FLUIDO SE INDICA POR UNA SEÑAL, TAL COMO UN CAMBIO DE COLOR O UNA VELOCIDAD DE CAMBIO DE COLOR, CONSECUENTE A LA REACCION INDICADOR. LA INVENCION TAMBIEN INCLUYE UN JUEGO DE MATERIALES UTILES PARA LLEVAR A CABO EL METODO DE LA INVENCION.
ES198787303661T 1986-05-12 1987-04-24 Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando. Expired - Lifetime ES2025654T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/861,818 US4810631A (en) 1986-05-12 1986-05-12 Signal enhancement in immunoassay by modulation of chemical catalysis

Publications (1)

Publication Number Publication Date
ES2025654T3 true ES2025654T3 (es) 1992-04-01

Family

ID=25336850

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787303661T Expired - Lifetime ES2025654T3 (es) 1986-05-12 1987-04-24 Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando.

Country Status (12)

Country Link
US (1) US4810631A (es)
EP (1) EP0245981B1 (es)
JP (1) JPH063447B2 (es)
AU (1) AU596832B2 (es)
BR (1) BR8701435A (es)
CA (1) CA1271419A (es)
DE (1) DE3772840D1 (es)
DK (1) DK233987A (es)
ES (1) ES2025654T3 (es)
MX (1) MX167488B (es)
NZ (1) NZ219442A (es)
ZA (1) ZA871750B (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962024A (en) * 1988-08-11 1990-10-09 Becton, Dickinson And Company Signal enhancement in assay for an enzyme
CA2000678A1 (en) * 1988-11-17 1990-05-17 John E. Reardon Chromogenic substrate for esterase and immunoassay using same
IE900688A1 (en) * 1989-03-08 1991-02-13 Becton Dickinson Co Signal enhancement in magnetic immunoassay by inhibition of¹enzymatic catalysis
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
GB8927503D0 (en) * 1989-12-04 1990-02-07 Kronem Systems Inc Enzyme-amplified lanthanide chelate luminescence
US5972630A (en) * 1991-08-19 1999-10-26 Dade Behring Marburg Gmbh Homogeneous immunoassays using enzyme inhibitors
US5279937A (en) * 1992-04-30 1994-01-18 Detechnology Canada Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays
US5843666A (en) * 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners
WO2001056456A2 (en) * 2000-02-02 2001-08-09 Cygene, Inc. Methods and compositions for detection of disease
WO2002033396A1 (en) * 2000-10-14 2002-04-25 Saunders Alex M Ligand based solution assay for low concentration analytes
GB0310270D0 (en) * 2003-05-03 2003-06-11 Univ Edinburgh Biomolecular devices
WO2007064300A1 (en) * 2005-11-30 2007-06-07 Agency For Science, Technology And Research Electrochemical method of detecting an analyte
US9945133B2 (en) * 2011-11-15 2018-04-17 New Standards Manufacturing Co. System and method for aligning and leveling tile
EP2981618B1 (en) 2013-04-01 2019-08-28 Becton, Dickinson and Company Methods and kits for the diagnosis of influenza

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160645A (en) * 1977-07-14 1979-07-10 Syva Company Catalyst mediated competitive protein binding assay
US4233144A (en) * 1979-04-16 1980-11-11 Technicon Instruments Corporation Electrode for voltammetric immunoassay
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
US4375972A (en) * 1979-10-17 1983-03-08 Allied Corporation Heterogeneous chemiluminescent immunoassays utilizing metallo porphyrin tag
US4463090A (en) * 1981-09-30 1984-07-31 Harris Curtis C Cascade amplification enzyme immunoassay
GB8432069D0 (en) * 1984-12-19 1985-01-30 Iq Bio Ltd Apparatus for immunoassay
US4865972A (en) * 1986-08-22 1989-09-12 Westinghouse Electric Corp. Antibody-based bioassay for enzyme-inducing chemicals

Also Published As

Publication number Publication date
US4810631A (en) 1989-03-07
DK233987A (da) 1987-11-13
JPH063447B2 (ja) 1994-01-12
ZA871750B (en) 1987-08-31
EP0245981B1 (en) 1991-09-11
EP0245981A2 (en) 1987-11-19
CA1271419A (en) 1990-07-10
BR8701435A (pt) 1988-01-26
DE3772840D1 (de) 1991-10-17
AU7050087A (en) 1987-11-19
EP0245981A3 (en) 1988-05-25
DK233987D0 (da) 1987-05-07
AU596832B2 (en) 1990-05-17
MX167488B (es) 1993-03-25
JPS62274261A (ja) 1987-11-28
NZ219442A (en) 1989-03-29

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