ES2012288A6 - Preparado de la enzima 5'-fosfodiesterasa y procedimiento para su produccion. - Google Patents

Preparado de la enzima 5'-fosfodiesterasa y procedimiento para su produccion.

Info

Publication number
ES2012288A6
ES2012288A6 ES8900919A ES8900919A ES2012288A6 ES 2012288 A6 ES2012288 A6 ES 2012288A6 ES 8900919 A ES8900919 A ES 8900919A ES 8900919 A ES8900919 A ES 8900919A ES 2012288 A6 ES2012288 A6 ES 2012288A6
Authority
ES
Spain
Prior art keywords
enzyme preparation
production
phosphodiesterase enzyme
phosphodiesterase
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8900919A
Other languages
English (en)
Inventor
Linda K Bowles
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genencor International Wisconsin Inc
Original Assignee
Enzyme Bio Systems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzyme Bio Systems Ltd filed Critical Enzyme Bio Systems Ltd
Publication of ES2012288A6 publication Critical patent/ES2012288A6/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)

Abstract

PREPARADO DE LA ENZIMA 5''-FOSFODIESTERASA Y PROCEDIMIENTO PARA SU PRODUCCION. EL PREPARADO CONTIENE 5''-FOSFODIESTERASA Y 5''-NUCLEOTIDASA, EXTRAIDAS DE BROTES DE MALTA DE CEBADA, EN UNA PROPORCION MINIMA DE 7:1. EL PROCEDIMIENTO CONSISTE EN MEZCLAR CON AGUA BROTES DE MALTA DE CEBADA MOLIDOS, SEPARAR LAS PARTICULAS MOLIDAS DE MAYOR TAMAÑO, DEJANDO UNA SUSPENSION ACUOSA DE BROTES FINAMENTE DIVIDIDOS, SEPARAR ESTOS ULTIMOS PARA PROPORCIONAR UN EXTRACTO ENZIMATICO ACUOSO CON MENOS DE 1% EN PESO DE SOLIDOS INSOLUBLES EN AGUA, CONCENTRAR DICHO EXTRACTO POR ULTRAFILTRACION Y CALENTARLO A 60-65GC, A PH 4,3-5,4, DURANTE 15-60 MINUTOS. EL PREPARADO RESULTANTE ES UTIL PARA CATALIZAR LA OBTENCION DE 5''-NUCLEOTIDOS HIDROLIZANDO ACIDO RIBONUCLEICO, MEJORAR LA FILTRABILIDAD DE CALDOS MICROBIANOS Y REDUCIR SU TENDENCIA A ENSUCIAR RESINAS DE INTERCAMBIO IONICO AL PASAR A SU TRAVES.
ES8900919A 1988-03-25 1989-03-14 Preparado de la enzima 5'-fosfodiesterasa y procedimiento para su produccion. Expired - Lifetime ES2012288A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17282488A 1988-03-25 1988-03-25

Publications (1)

Publication Number Publication Date
ES2012288A6 true ES2012288A6 (es) 1990-03-01

Family

ID=22629387

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8900919A Expired - Lifetime ES2012288A6 (es) 1988-03-25 1989-03-14 Preparado de la enzima 5'-fosfodiesterasa y procedimiento para su produccion.

Country Status (16)

Country Link
EP (1) EP0406319B1 (es)
JP (1) JPH04504649A (es)
KR (1) KR900700596A (es)
AT (1) ATE118248T1 (es)
AU (1) AU625555B2 (es)
CA (1) CA1313636C (es)
DE (1) DE68921070T2 (es)
DK (1) DK230290A (es)
ES (1) ES2012288A6 (es)
FI (1) FI904681A0 (es)
IE (1) IE890835L (es)
MX (1) MX171136B (es)
NZ (1) NZ228178A (es)
PH (1) PH25082A (es)
WO (1) WO1989009276A1 (es)
ZA (1) ZA891699B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100222638B1 (ko) * 1997-01-20 1999-10-01 배희동 종자를 이용한 효소산물 및 사료원료의 생산
AU2003902303A0 (en) * 2003-05-14 2003-05-29 Protech Research Pty Ltd Extracting and purifying enzymes
FR2905562B1 (fr) * 2006-09-12 2009-07-17 Lesaffre Et Compangie Sa Nouvelle preparation de phosphodiesterase d'orgine vegetale
US20200178580A1 (en) * 2016-12-16 2020-06-11 Malteurop Groupe Malt sprouts extracts and their uses

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB940660A (en) * 1961-02-04 1963-10-30 Waldhof Zellstoff Fab Improvements in and relating to the production of 5-nucleotides
FR1438228A (fr) * 1963-10-03 1966-05-13 Schwarz Biores Digestion enzymatique d'acide nucléique

Also Published As

Publication number Publication date
IE890835L (en) 1989-09-25
FI904681A7 (fi) 1990-09-24
WO1989009276A1 (en) 1989-10-05
NZ228178A (en) 1991-07-26
EP0406319A1 (en) 1991-01-09
DK230290D0 (da) 1990-09-24
PH25082A (en) 1991-02-19
CA1313636C (en) 1993-02-16
FI904681A0 (fi) 1990-09-24
EP0406319A4 (en) 1991-09-25
AU625555B2 (en) 1992-07-16
ATE118248T1 (de) 1995-02-15
DK230290A (da) 1990-09-24
AU3421389A (en) 1989-10-16
KR900700596A (ko) 1990-08-16
EP0406319B1 (en) 1995-02-08
JPH04504649A (ja) 1992-08-20
MX171136B (es) 1993-10-04
ZA891699B (en) 1989-11-29
DE68921070D1 (de) 1995-03-23
DE68921070T2 (de) 1995-06-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000201